streptavidin horseradish peroxidase complex  (Thermo Fisher)


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    Structured Review

    Thermo Fisher streptavidin horseradish peroxidase complex
    Streptavidin Horseradish Peroxidase Complex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin horseradish peroxidase complex/product/Thermo Fisher
    Average 97 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    streptavidin horseradish peroxidase complex - by Bioz Stars, 2020-04
    97/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Proteomic Analysis of Lung Tissue in a Rat Acute Lung Injury Model: Identification of PRDX1 as a Promoter of Inflammation
    Article Snippet: Paragraph title: 2.12. Immunohistochemistry Analysis (IHC) ... After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, South San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed, South San Francisco, CA).

    Article Title: NF-κB induces miR-148a to sustain TGF-β/Smad signaling activation in glioblastoma
    Article Snippet: Paragraph title: Immunohistochemistry ... After washing, the tissue sections were treated with biotinylated anti-rabbit/mouse secondary antibody (Zymed, San Francisco, CA, USA), followed by incubation with streptavidin–horseradish peroxidase complex (Zymed).

    Article Title: microRNA‐222 promotes colorectal cancer cell migration and invasion by targeting MST3
    Article Snippet: Paragraph title: Immunohistochemistry and assessment ... The tissue sections were treated with biotinylated anti‐rabbit secondary antibody (Thermo Fisher Scientific), followed by further incubation with a streptavidin–horseradish peroxidase complex (Thermo Fisher Scientific).

    Article Title: AEG-1 is involved in hypoxia-induced autophagy and decreases chemosensitivity in T-cell lymphoma
    Article Snippet: Paragraph title: Immunohistochemical assay ... After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed Laboratories Inc., South San Francisco, CA, USA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: The expression of AEG-1 and Cyclin D1 in human bladder urothelial carcinoma and their clinicopathological significance
    Article Snippet: Paragraph title: Immunohistochemical (IHC) analysis ... After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (1:250; Zymed Laboratories Inc; San Francisco, CA USA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed Laboratories Inc; San Francisco, CA USA).

    Article Title: Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy
    Article Snippet: Paragraph title: Immunohistochemistry ... After washing, tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed, San Francisco, CA).

    Article Title: Clinical significance of SOX9 in human non-small cell lung cancer progression and overall patient survival
    Article Snippet: Paragraph title: Immunohistochemistry (IHC) ... After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, USA) followed by a further incubation with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: Combined upregulation of matrix metalloproteinase-1 and proteinase-activated receptor-1 predicts unfavorable prognosis in human nasopharyngeal carcinoma
    Article Snippet: Paragraph title: Immunohistochemistry analysis ... After washing in tris(hydroxymethyl) aminomethane (Tris)-buffered saline (TBS) (Sigma Aldrich Corp, St Louis, MO, USA), the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Antibody; #sc-2775, Santa Cruz Biotechnology, Inc), followed by further incubation with streptavidin–horseradish peroxidase complex (Life Technologies Corp, Carlsbad, CA, USA).

    Article Title: Oncoprotein Bmi-1 Renders Apoptotic Resistance to Glioma Cells through Activation of the IKK-Nuclear Factor-?B Pathway
    Article Snippet: Paragraph title: Immunohistochemistry ... After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer
    Article Snippet: Paragraph title: Immunohistochemistry ... After washing, tissue sections were incubated with biotinylated anti-rabbit secondary antibody (Zymed), then with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: Utility of SAM68 in the progression and prognosis for bladder cancer
    Article Snippet: Paragraph title: Immunohistochemistry ... After washing, tissue pieces were treated with biotinylated anti-rabbit secondary antibody (Zymed), followed by further incubation with streptavidin -horseradish peroxidase complex (Zymed).

    Article Title: Wnt5a induces and maintains prostate cancer cells dormancy in bone
    Article Snippet: Paragraph title: IHC ... After washing, the tissue sections were treated with biotinylated anti-rabbit or anti-mouse secondary antibody (Zymed), followed by further incubation with streptavidin–horseradish peroxidase complex (Zymed).

    Binding Assay:

    Article Title: Proteomic Analysis of Lung Tissue in a Rat Acute Lung Injury Model: Identification of PRDX1 as a Promoter of Inflammation
    Article Snippet: The sections were treated with 3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity, followed by incubation with 1% bovine serum albumin to block nonspecific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, South San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed, South San Francisco, CA).

    Article Title: NF-κB induces miR-148a to sustain TGF-β/Smad signaling activation in glioblastoma
    Article Snippet: The sections were treated with 3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity, followed by incubation with 1% bovine serum albumin to block nonspecific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit/mouse secondary antibody (Zymed, San Francisco, CA, USA), followed by incubation with streptavidin–horseradish peroxidase complex (Zymed).

    Article Title: The expression of AEG-1 and Cyclin D1 in human bladder urothelial carcinoma and their clinicopathological significance
    Article Snippet: When the pressure reached 0.14 MP, time 1 min, and then cooled it 60 min. PBS buffer solution washed it 3 times × 5 min 1% bovine serum albumin (Gibco Life Technologies, Rockville, MD, USA) was used to block nonspecific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (1:250; Zymed Laboratories Inc; San Francisco, CA USA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed Laboratories Inc; San Francisco, CA USA).

    Article Title: Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy
    Article Snippet: The sections were submerged into EDTA antigenic retrieval buffer and microwaved for antigenic retrieval, after which they were treated with 3% hydrogen peroxide in methanol to quench endogenous peroxidase activity, followed by incubation with 1% bovine serum albumin to block nonspecific binding. .. After washing, tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed, San Francisco, CA).

    Article Title: Clinical significance of SOX9 in human non-small cell lung cancer progression and overall patient survival
    Article Snippet: The sections were treated with 3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity, followed by incubation in 1% bovine serum albumin to block non-specific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, USA) followed by a further incubation with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: Oncoprotein Bmi-1 Renders Apoptotic Resistance to Glioma Cells through Activation of the IKK-Nuclear Factor-?B Pathway
    Article Snippet: The sections were treated with 3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity, followed by incubation with 1% bovine serum albumin to block nonspecific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer
    Article Snippet: Sections were then submerged in EDTA antigenic retrieval buffer and microwaved for antigenic retrieval, treated with 3% hydrogen peroxide in methanol to quench endogenous peroxidase activity, and incubated with 1% bovine serum albumin to block nonspecific binding. .. After washing, tissue sections were incubated with biotinylated anti-rabbit secondary antibody (Zymed), then with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: Wnt5a induces and maintains prostate cancer cells dormancy in bone
    Article Snippet: The sections were treated with 3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity, followed by incubation with 1% BSA to block nonspecific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit or anti-mouse secondary antibody (Zymed), followed by further incubation with streptavidin–horseradish peroxidase complex (Zymed).

    Negative Control:

    Article Title: Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy
    Article Snippet: Normal goat serum was used as a negative control. .. After washing, tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed, San Francisco, CA).

    Article Title: Combined upregulation of matrix metalloproteinase-1 and proteinase-activated receptor-1 predicts unfavorable prognosis in human nasopharyngeal carcinoma
    Article Snippet: After washing in tris(hydroxymethyl) aminomethane (Tris)-buffered saline (TBS) (Sigma Aldrich Corp, St Louis, MO, USA), the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Antibody; #sc-2775, Santa Cruz Biotechnology, Inc), followed by further incubation with streptavidin–horseradish peroxidase complex (Life Technologies Corp, Carlsbad, CA, USA). .. In each immunohistochemistry run, noncancerous nasopharyngeal specimens were used as control tissues, and omission of the primary antibody served as a negative control.

    Article Title: GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer
    Article Snippet: Sections were then incubated with anti-GOLPH3 rabbit polyclonal antibody (1:100, Abcam, ab69179) at 37°C for 40 min. Normal goat serum was used as a negative control. .. After washing, tissue sections were incubated with biotinylated anti-rabbit secondary antibody (Zymed), then with streptavidin-horseradish peroxidase complex (Zymed).

    Blocking Assay:

    Article Title: Proteomic Analysis of Lung Tissue in a Rat Acute Lung Injury Model: Identification of PRDX1 as a Promoter of Inflammation
    Article Snippet: The sections were treated with 3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity, followed by incubation with 1% bovine serum albumin to block nonspecific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, South San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed, South San Francisco, CA).

    Article Title: NF-κB induces miR-148a to sustain TGF-β/Smad signaling activation in glioblastoma
    Article Snippet: The sections were treated with 3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity, followed by incubation with 1% bovine serum albumin to block nonspecific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit/mouse secondary antibody (Zymed, San Francisco, CA, USA), followed by incubation with streptavidin–horseradish peroxidase complex (Zymed).

    Article Title: The expression of AEG-1 and Cyclin D1 in human bladder urothelial carcinoma and their clinicopathological significance
    Article Snippet: When the pressure reached 0.14 MP, time 1 min, and then cooled it 60 min. PBS buffer solution washed it 3 times × 5 min 1% bovine serum albumin (Gibco Life Technologies, Rockville, MD, USA) was used to block nonspecific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (1:250; Zymed Laboratories Inc; San Francisco, CA USA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed Laboratories Inc; San Francisco, CA USA).

    Article Title: Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy
    Article Snippet: The sections were submerged into EDTA antigenic retrieval buffer and microwaved for antigenic retrieval, after which they were treated with 3% hydrogen peroxide in methanol to quench endogenous peroxidase activity, followed by incubation with 1% bovine serum albumin to block nonspecific binding. .. After washing, tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed, San Francisco, CA).

    Article Title: Clinical significance of SOX9 in human non-small cell lung cancer progression and overall patient survival
    Article Snippet: The sections were treated with 3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity, followed by incubation in 1% bovine serum albumin to block non-specific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, USA) followed by a further incubation with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: Oncoprotein Bmi-1 Renders Apoptotic Resistance to Glioma Cells through Activation of the IKK-Nuclear Factor-?B Pathway
    Article Snippet: The sections were treated with 3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity, followed by incubation with 1% bovine serum albumin to block nonspecific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer
    Article Snippet: Sections were then submerged in EDTA antigenic retrieval buffer and microwaved for antigenic retrieval, treated with 3% hydrogen peroxide in methanol to quench endogenous peroxidase activity, and incubated with 1% bovine serum albumin to block nonspecific binding. .. After washing, tissue sections were incubated with biotinylated anti-rabbit secondary antibody (Zymed), then with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: Wnt5a induces and maintains prostate cancer cells dormancy in bone
    Article Snippet: The sections were treated with 3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity, followed by incubation with 1% BSA to block nonspecific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit or anti-mouse secondary antibody (Zymed), followed by further incubation with streptavidin–horseradish peroxidase complex (Zymed).

    Incubation:

    Article Title: Proteomic Analysis of Lung Tissue in a Rat Acute Lung Injury Model: Identification of PRDX1 as a Promoter of Inflammation
    Article Snippet: .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, South San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed, South San Francisco, CA). ..

    Article Title: NF-κB induces miR-148a to sustain TGF-β/Smad signaling activation in glioblastoma
    Article Snippet: .. After washing, the tissue sections were treated with biotinylated anti-rabbit/mouse secondary antibody (Zymed, San Francisco, CA, USA), followed by incubation with streptavidin–horseradish peroxidase complex (Zymed). ..

    Article Title: microRNA‐222 promotes colorectal cancer cell migration and invasion by targeting MST3
    Article Snippet: .. The tissue sections were treated with biotinylated anti‐rabbit secondary antibody (Thermo Fisher Scientific), followed by further incubation with a streptavidin–horseradish peroxidase complex (Thermo Fisher Scientific). ..

    Article Title: AEG-1 is involved in hypoxia-induced autophagy and decreases chemosensitivity in T-cell lymphoma
    Article Snippet: .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed Laboratories Inc., South San Francisco, CA, USA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed). ..

    Article Title: The expression of AEG-1 and Cyclin D1 in human bladder urothelial carcinoma and their clinicopathological significance
    Article Snippet: .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (1:250; Zymed Laboratories Inc; San Francisco, CA USA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed Laboratories Inc; San Francisco, CA USA). ..

    Article Title: Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy
    Article Snippet: .. After washing, tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed, San Francisco, CA). ..

    Article Title: Clinical significance of SOX9 in human non-small cell lung cancer progression and overall patient survival
    Article Snippet: .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, USA) followed by a further incubation with streptavidin-horseradish peroxidase complex (Zymed). .. The tissue sections were immersed in 3-amino-9-ethyl carbazole and counterstained using 10% Mayer's hematoxylin, dehydrated, and mounted in Crystal Mount (Sigma).

    Article Title: Combined upregulation of matrix metalloproteinase-1 and proteinase-activated receptor-1 predicts unfavorable prognosis in human nasopharyngeal carcinoma
    Article Snippet: .. After washing in tris(hydroxymethyl) aminomethane (Tris)-buffered saline (TBS) (Sigma Aldrich Corp, St Louis, MO, USA), the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Antibody; #sc-2775, Santa Cruz Biotechnology, Inc), followed by further incubation with streptavidin–horseradish peroxidase complex (Life Technologies Corp, Carlsbad, CA, USA). .. The tissue sections were immersed in 3-amino-9-ethyl carbazole (Chemical Industry Co., Ltd.) and counterstained with 10% Mayer’s hematoxylin (Chemical Industry Co., Ltd.), dehydrated, and mounted in Crystal Mount™ Aqueous Mounting Medium (Chemical Industry Co., Ltd).

    Article Title: Oncoprotein Bmi-1 Renders Apoptotic Resistance to Glioma Cells through Activation of the IKK-Nuclear Factor-?B Pathway
    Article Snippet: .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed). ..

    Article Title: Utility of SAM68 in the progression and prognosis for bladder cancer
    Article Snippet: .. After washing, tissue pieces were treated with biotinylated anti-rabbit secondary antibody (Zymed), followed by further incubation with streptavidin -horseradish peroxidase complex (Zymed). ..

    Article Title: Wnt5a induces and maintains prostate cancer cells dormancy in bone
    Article Snippet: .. After washing, the tissue sections were treated with biotinylated anti-rabbit or anti-mouse secondary antibody (Zymed), followed by further incubation with streptavidin–horseradish peroxidase complex (Zymed). .. Staining of tissue sections was performed using 3,3′-diaminobenzidine.

    Formalin-fixed Paraffin-Embedded:

    Article Title: microRNA‐222 promotes colorectal cancer cell migration and invasion by targeting MST3
    Article Snippet: The tissue sections were treated with biotinylated anti‐rabbit secondary antibody (Thermo Fisher Scientific), followed by further incubation with a streptavidin–horseradish peroxidase complex (Thermo Fisher Scientific). .. The degree of immunostaining on the formalin‐fixed, paraffin‐embedded sections was reviewed and scored independently by two observers based on the proportion of positively stained cells and the intensity of the staining.

    Article Title: Clinical significance of SOX9 in human non-small cell lung cancer progression and overall patient survival
    Article Snippet: After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, USA) followed by a further incubation with streptavidin-horseradish peroxidase complex (Zymed). .. The degree of immunostaining of formalin-fixed, paraffin-embedded sections was viewed and scored separately by two independent investigators, who were blinded to the histopathological features and patient details of the samples.

    Article Title: Oncoprotein Bmi-1 Renders Apoptotic Resistance to Glioma Cells through Activation of the IKK-Nuclear Factor-?B Pathway
    Article Snippet: After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed). .. The degree of immunostaining of formalin-fixed, paraffin-embedded sections was reviewed and scored independently by three observers, based on both the proportion of positively stained tumor cells and the intensity of staining.

    Article Title: Wnt5a induces and maintains prostate cancer cells dormancy in bone
    Article Snippet: After washing, the tissue sections were treated with biotinylated anti-rabbit or anti-mouse secondary antibody (Zymed), followed by further incubation with streptavidin–horseradish peroxidase complex (Zymed). .. The degree of immunostaining of formalin-fixed, paraffin-embedded sections was reviewed and scored independently by two independent investigators, based on both the proportion of positively stained tumor cells and the intensity of staining.

    Activity Assay:

    Article Title: Proteomic Analysis of Lung Tissue in a Rat Acute Lung Injury Model: Identification of PRDX1 as a Promoter of Inflammation
    Article Snippet: The sections were treated with 3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity, followed by incubation with 1% bovine serum albumin to block nonspecific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, South San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed, South San Francisco, CA).

    Article Title: NF-κB induces miR-148a to sustain TGF-β/Smad signaling activation in glioblastoma
    Article Snippet: The sections were treated with 3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity, followed by incubation with 1% bovine serum albumin to block nonspecific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit/mouse secondary antibody (Zymed, San Francisco, CA, USA), followed by incubation with streptavidin–horseradish peroxidase complex (Zymed).

    Article Title: microRNA‐222 promotes colorectal cancer cell migration and invasion by targeting MST3
    Article Snippet: Endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 10 min. .. The tissue sections were treated with biotinylated anti‐rabbit secondary antibody (Thermo Fisher Scientific), followed by further incubation with a streptavidin–horseradish peroxidase complex (Thermo Fisher Scientific).

    Article Title: Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy
    Article Snippet: The sections were submerged into EDTA antigenic retrieval buffer and microwaved for antigenic retrieval, after which they were treated with 3% hydrogen peroxide in methanol to quench endogenous peroxidase activity, followed by incubation with 1% bovine serum albumin to block nonspecific binding. .. After washing, tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed, San Francisco, CA).

    Article Title: Clinical significance of SOX9 in human non-small cell lung cancer progression and overall patient survival
    Article Snippet: The sections were treated with 3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity, followed by incubation in 1% bovine serum albumin to block non-specific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, USA) followed by a further incubation with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: Oncoprotein Bmi-1 Renders Apoptotic Resistance to Glioma Cells through Activation of the IKK-Nuclear Factor-?B Pathway
    Article Snippet: The sections were treated with 3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity, followed by incubation with 1% bovine serum albumin to block nonspecific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer
    Article Snippet: Sections were then submerged in EDTA antigenic retrieval buffer and microwaved for antigenic retrieval, treated with 3% hydrogen peroxide in methanol to quench endogenous peroxidase activity, and incubated with 1% bovine serum albumin to block nonspecific binding. .. After washing, tissue sections were incubated with biotinylated anti-rabbit secondary antibody (Zymed), then with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: Wnt5a induces and maintains prostate cancer cells dormancy in bone
    Article Snippet: The sections were treated with 3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity, followed by incubation with 1% BSA to block nonspecific binding. .. After washing, the tissue sections were treated with biotinylated anti-rabbit or anti-mouse secondary antibody (Zymed), followed by further incubation with streptavidin–horseradish peroxidase complex (Zymed).

    Immunostaining:

    Article Title: microRNA‐222 promotes colorectal cancer cell migration and invasion by targeting MST3
    Article Snippet: The tissue sections were treated with biotinylated anti‐rabbit secondary antibody (Thermo Fisher Scientific), followed by further incubation with a streptavidin–horseradish peroxidase complex (Thermo Fisher Scientific). .. The degree of immunostaining on the formalin‐fixed, paraffin‐embedded sections was reviewed and scored independently by two observers based on the proportion of positively stained cells and the intensity of the staining.

    Article Title: Clinical significance of SOX9 in human non-small cell lung cancer progression and overall patient survival
    Article Snippet: After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, USA) followed by a further incubation with streptavidin-horseradish peroxidase complex (Zymed). .. The degree of immunostaining of formalin-fixed, paraffin-embedded sections was viewed and scored separately by two independent investigators, who were blinded to the histopathological features and patient details of the samples.

    Article Title: Combined upregulation of matrix metalloproteinase-1 and proteinase-activated receptor-1 predicts unfavorable prognosis in human nasopharyngeal carcinoma
    Article Snippet: After washing in tris(hydroxymethyl) aminomethane (Tris)-buffered saline (TBS) (Sigma Aldrich Corp, St Louis, MO, USA), the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Antibody; #sc-2775, Santa Cruz Biotechnology, Inc), followed by further incubation with streptavidin–horseradish peroxidase complex (Life Technologies Corp, Carlsbad, CA, USA). .. Following the hematoxylin counterstaining, immunostaining was scored by two independent observers who were blinded to the clinicopathological parameters and clinical outcomes of the patients.

    Article Title: Oncoprotein Bmi-1 Renders Apoptotic Resistance to Glioma Cells through Activation of the IKK-Nuclear Factor-?B Pathway
    Article Snippet: After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed). .. The degree of immunostaining of formalin-fixed, paraffin-embedded sections was reviewed and scored independently by three observers, based on both the proportion of positively stained tumor cells and the intensity of staining.

    Article Title: Utility of SAM68 in the progression and prognosis for bladder cancer
    Article Snippet: After washing, tissue pieces were treated with biotinylated anti-rabbit secondary antibody (Zymed), followed by further incubation with streptavidin -horseradish peroxidase complex (Zymed). .. The degree of immunostaining of paraffin-embedded sections was reviewed and scored independently by two observers based on the proportion of positively-stained tumor cells and the intensity of staining.

    Article Title: Wnt5a induces and maintains prostate cancer cells dormancy in bone
    Article Snippet: After washing, the tissue sections were treated with biotinylated anti-rabbit or anti-mouse secondary antibody (Zymed), followed by further incubation with streptavidin–horseradish peroxidase complex (Zymed). .. The degree of immunostaining of formalin-fixed, paraffin-embedded sections was reviewed and scored independently by two independent investigators, based on both the proportion of positively stained tumor cells and the intensity of staining.

    Expressing:

    Article Title: NF-κB induces miR-148a to sustain TGF-β/Smad signaling activation in glioblastoma
    Article Snippet: Immunohistochemistry Immunohistochemical (IHC) analysis was performed to study altered protein expression in 167 clinical glioma tissue sections. .. After washing, the tissue sections were treated with biotinylated anti-rabbit/mouse secondary antibody (Zymed, San Francisco, CA, USA), followed by incubation with streptavidin–horseradish peroxidase complex (Zymed).

    Article Title: AEG-1 is involved in hypoxia-induced autophagy and decreases chemosensitivity in T-cell lymphoma
    Article Snippet: Immunohistochemical assay Standard immunoperoxidase procedures were used to visualize AEG-1 and LC3-II expression, as previously described (Yan et al., ). .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed Laboratories Inc., South San Francisco, CA, USA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy
    Article Snippet: Immunohistochemistry Immunohistochemical analysis was performed to study altered protein expression in two human normal salivary gland tissues and 159 human salivary gland carcinomas tissues. .. After washing, tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed, San Francisco, CA).

    Article Title: GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer
    Article Snippet: Immunohistochemistry methods and scoring for GOLPH3 expression were done as previously described. .. After washing, tissue sections were incubated with biotinylated anti-rabbit secondary antibody (Zymed), then with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: Utility of SAM68 in the progression and prognosis for bladder cancer
    Article Snippet: Immunohistochemistry Immunohistochemistry (IHC) was performed to study altered SAM68 protein expression levels in 129 human MIBC tissues, as well as the ten pairs of MIBC tissue specimens and corresponding non-tumorous specimens, and eight paired of NMIBC and MIBC tissues. .. After washing, tissue pieces were treated with biotinylated anti-rabbit secondary antibody (Zymed), followed by further incubation with streptavidin -horseradish peroxidase complex (Zymed).

    Article Title: Wnt5a induces and maintains prostate cancer cells dormancy in bone
    Article Snippet: IHC IHC analysis was performed to study altered protein expression in human PCa tissues and bone tissues. .. After washing, the tissue sections were treated with biotinylated anti-rabbit or anti-mouse secondary antibody (Zymed), followed by further incubation with streptavidin–horseradish peroxidase complex (Zymed).

    Staining:

    Article Title: microRNA‐222 promotes colorectal cancer cell migration and invasion by targeting MST3
    Article Snippet: Immunohistochemistry and assessment Immunohistochemical staining was performed on 4 mm sections from 38 paraffin‐embedded specimens. .. The tissue sections were treated with biotinylated anti‐rabbit secondary antibody (Thermo Fisher Scientific), followed by further incubation with a streptavidin–horseradish peroxidase complex (Thermo Fisher Scientific).

    Article Title: Combined upregulation of matrix metalloproteinase-1 and proteinase-activated receptor-1 predicts unfavorable prognosis in human nasopharyngeal carcinoma
    Article Snippet: Immunohistochemistry analysis The expression patterns of the MMP-1 and PAR-1 proteins in the 266 NPC specimens and 100 noncancerous nasopharyngeal specimens were detected by immunohistochemical staining. .. After washing in tris(hydroxymethyl) aminomethane (Tris)-buffered saline (TBS) (Sigma Aldrich Corp, St Louis, MO, USA), the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Antibody; #sc-2775, Santa Cruz Biotechnology, Inc), followed by further incubation with streptavidin–horseradish peroxidase complex (Life Technologies Corp, Carlsbad, CA, USA).

    Article Title: Oncoprotein Bmi-1 Renders Apoptotic Resistance to Glioma Cells through Activation of the IKK-Nuclear Factor-?B Pathway
    Article Snippet: For negative controls, the rabbit anti-Bmi-1 antibody was replaced with normal goat serum, or the rabbit anti-Bmi-1 antibody was blocked with a recombinant Bmi-1 polypeptide by co-incubation at 4°C overnight preceding the immunohistochemical staining procedure. .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed).

    Article Title: GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer
    Article Snippet: After washing, tissue sections were incubated with biotinylated anti-rabbit secondary antibody (Zymed), then with streptavidin-horseradish peroxidase complex (Zymed). .. GOLPH3 staining was scored independently by two pathologists.

    Article Title: Utility of SAM68 in the progression and prognosis for bladder cancer
    Article Snippet: After washing, tissue pieces were treated with biotinylated anti-rabbit secondary antibody (Zymed), followed by further incubation with streptavidin -horseradish peroxidase complex (Zymed). .. The degree of immunostaining of paraffin-embedded sections was reviewed and scored independently by two observers based on the proportion of positively-stained tumor cells and the intensity of staining.

    Article Title: Wnt5a induces and maintains prostate cancer cells dormancy in bone
    Article Snippet: After washing, the tissue sections were treated with biotinylated anti-rabbit or anti-mouse secondary antibody (Zymed), followed by further incubation with streptavidin–horseradish peroxidase complex (Zymed). .. Staining of tissue sections was performed using 3,3′-diaminobenzidine.

    Recombinant:

    Article Title: Oncoprotein Bmi-1 Renders Apoptotic Resistance to Glioma Cells through Activation of the IKK-Nuclear Factor-?B Pathway
    Article Snippet: For negative controls, the rabbit anti-Bmi-1 antibody was replaced with normal goat serum, or the rabbit anti-Bmi-1 antibody was blocked with a recombinant Bmi-1 polypeptide by co-incubation at 4°C overnight preceding the immunohistochemical staining procedure. .. After washing, the tissue sections were treated with biotinylated anti-rabbit secondary antibody (Zymed, San Francisco, CA), followed by further incubation with streptavidin-horseradish peroxidase complex (Zymed).

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  • 87
    Thermo Fisher streptavidin horse radish peroxidase hrp
    Analysis of p53 binding to head-to-tail response element in cyclin B promoter using electrophoretic mobility shift assays. A , MCF10A, MCF10A/OD, and MCF10A/Δp53 cell lines were incubated with 10 ng/ml SN38 for 24 hours (+SN) or were left untreated (–SN). Binding reactions were prepared by incubating nuclear extracts or recombinant p53 with a biotin-labeled probe corresponding to the head-to-tail response element in the cyclin B promoter, in the presence (+) or absence (−) of a 200-fold molar excess of specific DNA (unlabeled probe). All binding reactions were carried out in the presence of a 100-fold molar excess of unlabeled non-specific DNA. Complexes were separated on 4% native polyacrylamide gel electrophoresis, transferred to positively charged nylon membrane, and visualized using a <t>streptavidin-horse</t> radish peroxidase conjugate. B , A parallel gel was transferred to nitrocellulose membrane and immunoblotted with p53-specific antibodies.
    Streptavidin Horse Radish Peroxidase Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin horse radish peroxidase hrp/product/Thermo Fisher
    Average 87 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    streptavidin horse radish peroxidase hrp - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    96
    Thermo Fisher avidin biotin complex horseradish peroxidase abc reagent
    Analysis of p53 binding to head-to-tail response element in cyclin B promoter using electrophoretic mobility shift assays. A , MCF10A, MCF10A/OD, and MCF10A/Δp53 cell lines were incubated with 10 ng/ml SN38 for 24 hours (+SN) or were left untreated (–SN). Binding reactions were prepared by incubating nuclear extracts or recombinant p53 with a biotin-labeled probe corresponding to the head-to-tail response element in the cyclin B promoter, in the presence (+) or absence (−) of a 200-fold molar excess of specific DNA (unlabeled probe). All binding reactions were carried out in the presence of a 100-fold molar excess of unlabeled non-specific DNA. Complexes were separated on 4% native polyacrylamide gel electrophoresis, transferred to positively charged nylon membrane, and visualized using a <t>streptavidin-horse</t> radish peroxidase conjugate. B , A parallel gel was transferred to nitrocellulose membrane and immunoblotted with p53-specific antibodies.
    Avidin Biotin Complex Horseradish Peroxidase Abc Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin complex horseradish peroxidase abc reagent/product/Thermo Fisher
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex horseradish peroxidase abc reagent - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    94
    Thermo Fisher avidin hrp
    Analysis of p53 binding to head-to-tail response element in cyclin B promoter using electrophoretic mobility shift assays. A , MCF10A, MCF10A/OD, and MCF10A/Δp53 cell lines were incubated with 10 ng/ml SN38 for 24 hours (+SN) or were left untreated (–SN). Binding reactions were prepared by incubating nuclear extracts or recombinant p53 with a biotin-labeled probe corresponding to the head-to-tail response element in the cyclin B promoter, in the presence (+) or absence (−) of a 200-fold molar excess of specific DNA (unlabeled probe). All binding reactions were carried out in the presence of a 100-fold molar excess of unlabeled non-specific DNA. Complexes were separated on 4% native polyacrylamide gel electrophoresis, transferred to positively charged nylon membrane, and visualized using a <t>streptavidin-horse</t> radish peroxidase conjugate. B , A parallel gel was transferred to nitrocellulose membrane and immunoblotted with p53-specific antibodies.
    Avidin Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin hrp/product/Thermo Fisher
    Average 94 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    avidin hrp - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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    Analysis of p53 binding to head-to-tail response element in cyclin B promoter using electrophoretic mobility shift assays. A , MCF10A, MCF10A/OD, and MCF10A/Δp53 cell lines were incubated with 10 ng/ml SN38 for 24 hours (+SN) or were left untreated (–SN). Binding reactions were prepared by incubating nuclear extracts or recombinant p53 with a biotin-labeled probe corresponding to the head-to-tail response element in the cyclin B promoter, in the presence (+) or absence (−) of a 200-fold molar excess of specific DNA (unlabeled probe). All binding reactions were carried out in the presence of a 100-fold molar excess of unlabeled non-specific DNA. Complexes were separated on 4% native polyacrylamide gel electrophoresis, transferred to positively charged nylon membrane, and visualized using a streptavidin-horse radish peroxidase conjugate. B , A parallel gel was transferred to nitrocellulose membrane and immunoblotted with p53-specific antibodies.

    Journal: PLoS ONE

    Article Title: p53 Dimers Associate with a Head-to-Tail Response Element to Repress Cyclin B Transcription

    doi: 10.1371/journal.pone.0042615

    Figure Lengend Snippet: Analysis of p53 binding to head-to-tail response element in cyclin B promoter using electrophoretic mobility shift assays. A , MCF10A, MCF10A/OD, and MCF10A/Δp53 cell lines were incubated with 10 ng/ml SN38 for 24 hours (+SN) or were left untreated (–SN). Binding reactions were prepared by incubating nuclear extracts or recombinant p53 with a biotin-labeled probe corresponding to the head-to-tail response element in the cyclin B promoter, in the presence (+) or absence (−) of a 200-fold molar excess of specific DNA (unlabeled probe). All binding reactions were carried out in the presence of a 100-fold molar excess of unlabeled non-specific DNA. Complexes were separated on 4% native polyacrylamide gel electrophoresis, transferred to positively charged nylon membrane, and visualized using a streptavidin-horse radish peroxidase conjugate. B , A parallel gel was transferred to nitrocellulose membrane and immunoblotted with p53-specific antibodies.

    Article Snippet: The electrophoretic mobility of the complexes was detected using streptavidin-horse radish peroxidase (HRP) and a chemiluminescent substrate (Thermo Scientific).

    Techniques: Binding Assay, Electrophoretic Mobility Shift Assay, Incubation, Recombinant, Labeling, Polyacrylamide Gel Electrophoresis

    Identification of genome-wide association of Klf9 in HT22 cell chromatin using chromatin-streptavidin precipitation sequencing. a HT22 [BirA/FLBIO-Klf9] cells express biotinylated Klf9. Whole cell extracts from the HT22 parent cell line (lane 1), HT22 [BirA] cells (lane 2) or HT22 [BirA/FLBIO-Klf9] (lane 3) were fractionated on 10% SDS-PAGE and analyzed by Western blotting using streptavidin-HRP. b Chromatin-streptavidin precipitation gives ~25-fold enrichment at the Klf13 promoter in HT22 [BirA/FLBIO-Klf9] cells compared with HT22 [BirA] cells. Precipitated DNA was analyzed by qPCR at the Klf13 promoter and intron (negative control region). The asterisk indicates a statistically significant difference by Student’s two-sample t -test ( p

    Journal: BMC Genomics

    Article Title: The Krüppel-like factor 9 cistrome in mouse hippocampal neurons reveals predominant transcriptional repression via proximal promoter binding

    doi: 10.1186/s12864-017-3640-7

    Figure Lengend Snippet: Identification of genome-wide association of Klf9 in HT22 cell chromatin using chromatin-streptavidin precipitation sequencing. a HT22 [BirA/FLBIO-Klf9] cells express biotinylated Klf9. Whole cell extracts from the HT22 parent cell line (lane 1), HT22 [BirA] cells (lane 2) or HT22 [BirA/FLBIO-Klf9] (lane 3) were fractionated on 10% SDS-PAGE and analyzed by Western blotting using streptavidin-HRP. b Chromatin-streptavidin precipitation gives ~25-fold enrichment at the Klf13 promoter in HT22 [BirA/FLBIO-Klf9] cells compared with HT22 [BirA] cells. Precipitated DNA was analyzed by qPCR at the Klf13 promoter and intron (negative control region). The asterisk indicates a statistically significant difference by Student’s two-sample t -test ( p

    Article Snippet: To detect FLBIO-Klf9 we incubated the membrane with Streptavidin-HRP (Thermo; diluted 1:1000) for 1 h. Immune or streptavidin-HRP complexes were revealed by chemiluminescence detection using Pierce ECL Substrate (ThermoFisher Scientific).

    Techniques: GWAS, Sequencing, SDS Page, Western Blot, Real-time Polymerase Chain Reaction, Negative Control