streptavidin conjugated to alkaline phosphatase  (Millipore)


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    Name:
    Streptavidin Alkaline
    Description:
    Suitable for use in enhanced chemiluminescence ECL
    Catalog Number:
    gerpn1234
    Price:
    None
    Applications:
    Streptavidin-alkaline phosphatase is prepared using a single-step glutaraldehyde coupling reaction to link calf intestinal alkaline phosphatase to streptavidin. Excess coupling reagent is removed using extensive dialysis. The final product concentration is optimized to a standard titer as determined by its binding to biotinylated Protein. As a result the final Protein concentration may vary minimally between batches.
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    Structured Review

    Millipore streptavidin conjugated to alkaline phosphatase
    Suitable for use in enhanced chemiluminescence ECL
    https://www.bioz.com/result/streptavidin conjugated to alkaline phosphatase/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    streptavidin conjugated to alkaline phosphatase - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Affinity Chromatography:

    Article Title: High-throughput Biotinylation of Proteins
    Article Snippet: .. Ampicillin (Sigma-Aldrich Chemical Company, St. Louis, MO) Anti-Fab-HRP antibody (Jackson ImmunoResearch Laboratories, West Grove, PA) Autoinducing medium (described in ( ); can be purchased from Novagen, Madison, WI) D-biotin (Sigma-Aldrich) BirA enzyme (Avidity, Boulder, CO) BugBuster detergent (Novagen) E. coli strain BL21 (DE3) (Novagen) EZ-Link® Sulfo-NHS-LC-biotin (Pierce Chemical Company, Rockford, IL; MW = 557n daltons) Immobilized metal affinity chromatography (IMAC) resin (Qiagen, Valencia, CA) LB+ampicillin: Luria Broth (10 gm yeast extract, 10 gm peptone, and 5 gm NaCl in one liter of water; autoclaved) plus 100 μg/ml ampicillin LB+ampicillin+chloramphenicol: LB+ampicillin plus 12.5 μg/mL chloramphenicol MagnaBind™ Streptavidin Beads (Pierce Chemical Company) pBirA Cmr biotinylation plasmid (Avidity) pMCSG16 and pMCSG17 vectors (described in ( ); available upon request) Phosphate Buffered Saline (PBS: 137 mM NaCl, 3 mM KCl, 8 mM Na2 HPO4 1.5 mM KH2 PO4 ) p-nitrophenyl phosphate (Sigma-Aldrich) Qiagen Gel Extraction Kit (Qiagen, Valencia, CA) Slide-A-Lyzer™ dialysis cassettes (Pierce) Sma I (New England Biolabs, Waverly, MA) Streptavidin (Sigma-Aldrich Chemical Company, St. Louis, MO) Streptavidin-alkaline phosphatase (Sigma-Aldrich) T4 DNA polymerase (Promega Corporation, Madison, WI) Zebra spin-columns (Pierce) .. To generate biotinylated proteins for affinity selection experiments, one can transfer the open reading frame (ORF) of a protein of interest into plasmids that contain both the AviTag biotinylation sequence and a six-histidine tag, at either the N- or C- terminus of the ORF.

    Gel Extraction:

    Article Title: High-throughput Biotinylation of Proteins
    Article Snippet: .. Ampicillin (Sigma-Aldrich Chemical Company, St. Louis, MO) Anti-Fab-HRP antibody (Jackson ImmunoResearch Laboratories, West Grove, PA) Autoinducing medium (described in ( ); can be purchased from Novagen, Madison, WI) D-biotin (Sigma-Aldrich) BirA enzyme (Avidity, Boulder, CO) BugBuster detergent (Novagen) E. coli strain BL21 (DE3) (Novagen) EZ-Link® Sulfo-NHS-LC-biotin (Pierce Chemical Company, Rockford, IL; MW = 557n daltons) Immobilized metal affinity chromatography (IMAC) resin (Qiagen, Valencia, CA) LB+ampicillin: Luria Broth (10 gm yeast extract, 10 gm peptone, and 5 gm NaCl in one liter of water; autoclaved) plus 100 μg/ml ampicillin LB+ampicillin+chloramphenicol: LB+ampicillin plus 12.5 μg/mL chloramphenicol MagnaBind™ Streptavidin Beads (Pierce Chemical Company) pBirA Cmr biotinylation plasmid (Avidity) pMCSG16 and pMCSG17 vectors (described in ( ); available upon request) Phosphate Buffered Saline (PBS: 137 mM NaCl, 3 mM KCl, 8 mM Na2 HPO4 1.5 mM KH2 PO4 ) p-nitrophenyl phosphate (Sigma-Aldrich) Qiagen Gel Extraction Kit (Qiagen, Valencia, CA) Slide-A-Lyzer™ dialysis cassettes (Pierce) Sma I (New England Biolabs, Waverly, MA) Streptavidin (Sigma-Aldrich Chemical Company, St. Louis, MO) Streptavidin-alkaline phosphatase (Sigma-Aldrich) T4 DNA polymerase (Promega Corporation, Madison, WI) Zebra spin-columns (Pierce) .. To generate biotinylated proteins for affinity selection experiments, one can transfer the open reading frame (ORF) of a protein of interest into plasmids that contain both the AviTag biotinylation sequence and a six-histidine tag, at either the N- or C- terminus of the ORF.

    Lysis:

    Article Title: Lipopolysaccharide Interaction with Cell Surface Toll-like Receptor 4-MD-2
    Article Snippet: .. Beads were washed in 5× diluted lysis buffer, and bound proteins were subjected to SDS-PAGE and Western blotting with streptavidin–alkaline phosphatase conjugate (Calbiochem). .. The primary reagents for immunoprobing were as follows: Sa2-8 for CD14; anti-LPS (Hycult Biotechnology) for LPS; anti–IkB-α (BD Biosciences); and anti-actin (Sigma-Aldrich).

    Western Blot:

    Article Title: Lipopolysaccharide Interaction with Cell Surface Toll-like Receptor 4-MD-2
    Article Snippet: .. Beads were washed in 5× diluted lysis buffer, and bound proteins were subjected to SDS-PAGE and Western blotting with streptavidin–alkaline phosphatase conjugate (Calbiochem). .. The primary reagents for immunoprobing were as follows: Sa2-8 for CD14; anti-LPS (Hycult Biotechnology) for LPS; anti–IkB-α (BD Biosciences); and anti-actin (Sigma-Aldrich).

    SDS Page:

    Article Title: Lipopolysaccharide Interaction with Cell Surface Toll-like Receptor 4-MD-2
    Article Snippet: .. Beads were washed in 5× diluted lysis buffer, and bound proteins were subjected to SDS-PAGE and Western blotting with streptavidin–alkaline phosphatase conjugate (Calbiochem). .. The primary reagents for immunoprobing were as follows: Sa2-8 for CD14; anti-LPS (Hycult Biotechnology) for LPS; anti–IkB-α (BD Biosciences); and anti-actin (Sigma-Aldrich).

    Plasmid Preparation:

    Article Title: High-throughput Biotinylation of Proteins
    Article Snippet: .. Ampicillin (Sigma-Aldrich Chemical Company, St. Louis, MO) Anti-Fab-HRP antibody (Jackson ImmunoResearch Laboratories, West Grove, PA) Autoinducing medium (described in ( ); can be purchased from Novagen, Madison, WI) D-biotin (Sigma-Aldrich) BirA enzyme (Avidity, Boulder, CO) BugBuster detergent (Novagen) E. coli strain BL21 (DE3) (Novagen) EZ-Link® Sulfo-NHS-LC-biotin (Pierce Chemical Company, Rockford, IL; MW = 557n daltons) Immobilized metal affinity chromatography (IMAC) resin (Qiagen, Valencia, CA) LB+ampicillin: Luria Broth (10 gm yeast extract, 10 gm peptone, and 5 gm NaCl in one liter of water; autoclaved) plus 100 μg/ml ampicillin LB+ampicillin+chloramphenicol: LB+ampicillin plus 12.5 μg/mL chloramphenicol MagnaBind™ Streptavidin Beads (Pierce Chemical Company) pBirA Cmr biotinylation plasmid (Avidity) pMCSG16 and pMCSG17 vectors (described in ( ); available upon request) Phosphate Buffered Saline (PBS: 137 mM NaCl, 3 mM KCl, 8 mM Na2 HPO4 1.5 mM KH2 PO4 ) p-nitrophenyl phosphate (Sigma-Aldrich) Qiagen Gel Extraction Kit (Qiagen, Valencia, CA) Slide-A-Lyzer™ dialysis cassettes (Pierce) Sma I (New England Biolabs, Waverly, MA) Streptavidin (Sigma-Aldrich Chemical Company, St. Louis, MO) Streptavidin-alkaline phosphatase (Sigma-Aldrich) T4 DNA polymerase (Promega Corporation, Madison, WI) Zebra spin-columns (Pierce) .. To generate biotinylated proteins for affinity selection experiments, one can transfer the open reading frame (ORF) of a protein of interest into plasmids that contain both the AviTag biotinylation sequence and a six-histidine tag, at either the N- or C- terminus of the ORF.

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  • 99
    Millipore fitc conjugated annexin v
    Effects of coptisine on cell viability and apoptosis in Hep3B cells. Hep3B cells were treated with the indicated concentrations of coptisine for 24 h. ( A ) After treated with coptisine, added the a 3-(4,5-dimethylthiazol-2-yl)-2-,5-diphenyltetrazolium bromide (MTT) solution, reacted for 2 h and then detected cell viability; ( B ) The morphology of Hep3B cells by coptisine were observed by phase-contrast microscopy (magnification, ×50); ( C ) The cells were harvested and stained with annexin V-fluorescein isothiocyanate <t>(FITC)</t> and propidium iodide (PI). After staining, cells were analyzed the apoptosis by flow cytometry. Annectin V-FITC results represent early apoptosis (lower right quadrant), and late apoptosis (upper right quadrant); ( D ) Statistical analysis of <t>annexin</t> V positive cells (** p
    Fitc Conjugated Annexin V, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated annexin v/product/Millipore
    Average 99 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    fitc conjugated annexin v - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore alkaline phosphatase conjugated streptavidin
    Effects of coptisine on cell viability and apoptosis in Hep3B cells. Hep3B cells were treated with the indicated concentrations of coptisine for 24 h. ( A ) After treated with coptisine, added the a 3-(4,5-dimethylthiazol-2-yl)-2-,5-diphenyltetrazolium bromide (MTT) solution, reacted for 2 h and then detected cell viability; ( B ) The morphology of Hep3B cells by coptisine were observed by phase-contrast microscopy (magnification, ×50); ( C ) The cells were harvested and stained with annexin V-fluorescein isothiocyanate <t>(FITC)</t> and propidium iodide (PI). After staining, cells were analyzed the apoptosis by flow cytometry. Annectin V-FITC results represent early apoptosis (lower right quadrant), and late apoptosis (upper right quadrant); ( D ) Statistical analysis of <t>annexin</t> V positive cells (** p
    Alkaline Phosphatase Conjugated Streptavidin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alkaline phosphatase conjugated streptavidin/product/Millipore
    Average 99 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    alkaline phosphatase conjugated streptavidin - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Effects of coptisine on cell viability and apoptosis in Hep3B cells. Hep3B cells were treated with the indicated concentrations of coptisine for 24 h. ( A ) After treated with coptisine, added the a 3-(4,5-dimethylthiazol-2-yl)-2-,5-diphenyltetrazolium bromide (MTT) solution, reacted for 2 h and then detected cell viability; ( B ) The morphology of Hep3B cells by coptisine were observed by phase-contrast microscopy (magnification, ×50); ( C ) The cells were harvested and stained with annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI). After staining, cells were analyzed the apoptosis by flow cytometry. Annectin V-FITC results represent early apoptosis (lower right quadrant), and late apoptosis (upper right quadrant); ( D ) Statistical analysis of annexin V positive cells (** p

    Journal: International Journal of Molecular Sciences

    Article Title: Induction of Apoptosis by Coptisine in Hep3B Hepatocellular Carcinoma Cells through Activation of the ROS-Mediated JNK Signaling Pathway

    doi: 10.3390/ijms21155502

    Figure Lengend Snippet: Effects of coptisine on cell viability and apoptosis in Hep3B cells. Hep3B cells were treated with the indicated concentrations of coptisine for 24 h. ( A ) After treated with coptisine, added the a 3-(4,5-dimethylthiazol-2-yl)-2-,5-diphenyltetrazolium bromide (MTT) solution, reacted for 2 h and then detected cell viability; ( B ) The morphology of Hep3B cells by coptisine were observed by phase-contrast microscopy (magnification, ×50); ( C ) The cells were harvested and stained with annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI). After staining, cells were analyzed the apoptosis by flow cytometry. Annectin V-FITC results represent early apoptosis (lower right quadrant), and late apoptosis (upper right quadrant); ( D ) Statistical analysis of annexin V positive cells (** p

    Article Snippet: The collected cells were re-suspended in binding buffer, and then stained with PI solution and FITC-conjugated annexin V, for 15 min, in dark, as previously described [ ].

    Techniques: MTT Assay, Microscopy, Staining, Flow Cytometry