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Boehringer Mannheim streptavidin conjugated alkaline phosphatase
Streptavidin Conjugated Alkaline Phosphatase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin conjugated alkaline phosphatase/product/Boehringer Mannheim
Average 92 stars, based on 9 article reviews
Price from $9.99 to $1999.99
streptavidin conjugated alkaline phosphatase - by Bioz Stars, 2020-09
92/100 stars

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Nucleic Acid Electrophoresis:

Article Title: The beta subunit of the high conductance calcium-activated potassium channel. Identification of residues involved in charybdotoxin binding.
Article Snippet: .. The immunoprecipitates were subjected to SDS-polyacrylamide gel electrophoresis, transferred to polyvinylidene difluoride membranes, and biotinylated proteins were detected by using streptavidin-conjugated alkaline phosphatase. ..

Incubation:

Article Title: Isolation and Characterization of a Phaseolus vulgaris Trypsin Inhibitor with Antiproliferative Activity on Leukemia and Lymphoma Cells
Article Snippet: .. After the integrase reaction, the biotinylated DNA immobilized on the wells was detected by incubation with streptavidin-conjugated alkaline phosphatase (Boehringer-Mannheim, Mannheim, Germany), followed by colorimetric detection with 1 mg/mL p -nitrophenyl phosphate in 10% diethanolamine buffer (pH 9.8) containing 0.5 mM MgCl2 . ..

Article Title: Role of Dendritic Cells in the Immune Response Induced by Mouse Mammary Tumor Virus Superantigen
Article Snippet: .. Finally, the plates were developed with 5-bromo-4-chloro-3-indolyl phosphate (Sigma) after incubation with a streptavidin-conjugated alkaline phosphatase (Boehringer Mannheim). ..

Article Title: Campesin, a thermostable antifungal peptide with highly potent antipathogenic activities.
Article Snippet: .. After the integrase reaction, the biotinylated DNA immobilized on the wells was detected by incubation with streptavidin-conjugated alkaline phosphatase (Boehringer Mannheim) followed by colorimetric detection with 1 mg/ml pnitrophenyl phosphate in 10% diethanolamine buffer, pH 9.8, containing 0.5 mM MgCl 2 . ..

Article Title: Campesin, a thermostable antifungal peptide with highly potent antipathogenic activities
Article Snippet: .. After the integrase reaction, the biotinylated DNA immobilized on the wells was detected by incubation with streptavidin-conjugated alkaline phosphatase (Boehringer Mannheim) followed by colorimetric detection with 1 mg/ml p -nitrophenyl phosphate in 10% diethanolamine buffer, pH 9.8, containing 0.5 mM MgCl2 . ..

Article Title: Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv "White Cloud Bean"
Article Snippet: .. After the reaction, biotinylated DNA immobilized on the wells was incubated with streptavidin-conjugated alkaline phosphatase (Boehringer-Mannheim, Mannheim, Germany), followed by colorimetric detection with 1 mg/ml p-nitrophenyl phosphate in 10% diethanolamine buffer (pH 9.8) containing 0.5 mM MgCl2 . .. The ribosome inactivating protein trichosanthin was employed as a positive control [ ].

Article Title: An antiproliferative ribonuclease from fruiting bodies of the wild mushroom Russula delica.
Article Snippet: .. After the integrase reaction, the biotinylated DNA immobilized on the wells was detected by incubation with streptavidin-conjugated alkaline phosphatase (BoehringerMannheim, Mannheim, Germany), followed by colorimetric detection with 1 mg/ml p-nitrophenyl phosphate in 10% diethanolamine buffer (pH 9.8) containing 0.5 mM MgCl2. .. The absorbance due to the alkaline phosphatase reaction was measured at 415 nm and the ribosome inactivating protein trichosanthin was used as a positive control [17].

Construct:

Article Title: The beta subunit of the high conductance calcium-activated potassium channel. Identification of residues involved in charybdotoxin binding.
Article Snippet: .. In marked contrast, all b subunit constructs in which one of the extracellular Cys residues had been changed to Ala gave a strong signal upon reaction with streptavidin-conjugated alkaline phosphatase (lanes 4–7). ..

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    Boehringer Mannheim streptavidin conjugated to alkaline phosphatase
    Cell surface expression of MS on elicited MPMs. Thioglycollate-elicited macrophages were harvested and subjected to cell-surface-specific biotinylation at 4°C followed by detergent extraction of cells and immunoadsorption with DS4 antiserum (lane 1). Alternatively, cell extracts were biotinylated and then processed for immunoadsorption (lane 2) to quantify total cellular expression of MS. The immunoprecipitates were subjected to SDS/PAGE and electroblot transfer, and the nitrocellulose strips were probed with <t>streptavidin-alkaline</t> phosphatase.
    Streptavidin Conjugated To Alkaline Phosphatase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 89/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin conjugated to alkaline phosphatase/product/Boehringer Mannheim
    Average 89 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    streptavidin conjugated to alkaline phosphatase - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

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    Cell surface expression of MS on elicited MPMs. Thioglycollate-elicited macrophages were harvested and subjected to cell-surface-specific biotinylation at 4°C followed by detergent extraction of cells and immunoadsorption with DS4 antiserum (lane 1). Alternatively, cell extracts were biotinylated and then processed for immunoadsorption (lane 2) to quantify total cellular expression of MS. The immunoprecipitates were subjected to SDS/PAGE and electroblot transfer, and the nitrocellulose strips were probed with streptavidin-alkaline phosphatase.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low density lipoprotein

    doi:

    Figure Lengend Snippet: Cell surface expression of MS on elicited MPMs. Thioglycollate-elicited macrophages were harvested and subjected to cell-surface-specific biotinylation at 4°C followed by detergent extraction of cells and immunoadsorption with DS4 antiserum (lane 1). Alternatively, cell extracts were biotinylated and then processed for immunoadsorption (lane 2) to quantify total cellular expression of MS. The immunoprecipitates were subjected to SDS/PAGE and electroblot transfer, and the nitrocellulose strips were probed with streptavidin-alkaline phosphatase.

    Article Snippet: Triton X-114, octyl glucoside, and streptavidin conjugated to alkaline phosphatase were from Boehringer Mannheim.

    Techniques: Expressing, Mass Spectrometry, SDS Page