streptavidin agarose conjugate  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88

    Structured Review

    Millipore streptavidin agarose conjugate
    Streptavidin Agarose Conjugate, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin agarose conjugate/product/Millipore
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavidin agarose conjugate - by Bioz Stars, 2020-04
    88/100 stars

    Images

    Related Articles

    Synthesized:

    Article Title: hnRNP A1 contacts exon 5 to promote exon 6 inclusion of apoptotic Fas gene
    Article Snippet: Wild type (CAAAGAGGAA) and mutant (CAAACUGG AA) of 5′ biotin tagged 10 nt RNA was chemically synthesized (Bioneer). .. Biotinylated RNA was covalently linked with Streptavidin agarose conjugate (Millipore) by mixing together for 1 h in buffer D (20 mM Tris–Cl, 100 mM KCl, 2 mM EDTA, 20 % glycerol, 0.5 mM DTT, 0.5 mM PMSF, pH 7.5).

    Article Title: Exon 9 skipping of apoptotic caspase-2 pre-mRNA is promoted by SRp20 through interaction with exon 8
    Article Snippet: .. Briefly, chemically synthesized 5’ biotin labeled wild type (UUCUUCAUCC) and mutant (UGAAGCGUCC) RNA were covalently linked with Streptavidin agarose conjugate (Millipore). .. Streptavidin agarose linked biotin-RNA was incubated with HeLa nuclear extract for 3.5 h at 4 °C in buffer D (20mM Tris-Cl, 100mM KCl, 2mM EDTA, 20% glycerol, 0.5mM DTT, 0.5mM PMSF, pH 7.5).

    Mutagenesis:

    Article Title: hnRNP A1 contacts exon 5 to promote exon 6 inclusion of apoptotic Fas gene
    Article Snippet: Wild type (CAAAGAGGAA) and mutant (CAAACUGG AA) of 5′ biotin tagged 10 nt RNA was chemically synthesized (Bioneer). .. Biotinylated RNA was covalently linked with Streptavidin agarose conjugate (Millipore) by mixing together for 1 h in buffer D (20 mM Tris–Cl, 100 mM KCl, 2 mM EDTA, 20 % glycerol, 0.5 mM DTT, 0.5 mM PMSF, pH 7.5).

    Article Title: Exon 9 skipping of apoptotic caspase-2 pre-mRNA is promoted by SRp20 through interaction with exon 8
    Article Snippet: .. Briefly, chemically synthesized 5’ biotin labeled wild type (UUCUUCAUCC) and mutant (UGAAGCGUCC) RNA were covalently linked with Streptavidin agarose conjugate (Millipore). .. Streptavidin agarose linked biotin-RNA was incubated with HeLa nuclear extract for 3.5 h at 4 °C in buffer D (20mM Tris-Cl, 100mM KCl, 2mM EDTA, 20% glycerol, 0.5mM DTT, 0.5mM PMSF, pH 7.5).

    Labeling:

    Article Title: Exon 9 skipping of apoptotic caspase-2 pre-mRNA is promoted by SRp20 through interaction with exon 8
    Article Snippet: .. Briefly, chemically synthesized 5’ biotin labeled wild type (UUCUUCAUCC) and mutant (UGAAGCGUCC) RNA were covalently linked with Streptavidin agarose conjugate (Millipore). .. Streptavidin agarose linked biotin-RNA was incubated with HeLa nuclear extract for 3.5 h at 4 °C in buffer D (20mM Tris-Cl, 100mM KCl, 2mM EDTA, 20% glycerol, 0.5mM DTT, 0.5mM PMSF, pH 7.5).

    Incubation:

    Article Title: hnRNP A1 contacts exon 5 to promote exon 6 inclusion of apoptotic Fas gene
    Article Snippet: Biotinylated RNA was covalently linked with Streptavidin agarose conjugate (Millipore) by mixing together for 1 h in buffer D (20 mM Tris–Cl, 100 mM KCl, 2 mM EDTA, 20 % glycerol, 0.5 mM DTT, 0.5 mM PMSF, pH 7.5). .. Streptavidin agarose linked biotin-RNA was incubated with HeLa nuclear extract for 3.5 h at 4 °C in buffer D. After washing with buffer for five times, we added SDS–PAGE loading dye and then boiled the sample.

    Article Title: Exon 9 skipping of apoptotic caspase-2 pre-mRNA is promoted by SRp20 through interaction with exon 8
    Article Snippet: Briefly, chemically synthesized 5’ biotin labeled wild type (UUCUUCAUCC) and mutant (UGAAGCGUCC) RNA were covalently linked with Streptavidin agarose conjugate (Millipore). .. Streptavidin agarose linked biotin-RNA was incubated with HeLa nuclear extract for 3.5 h at 4 °C in buffer D (20mM Tris-Cl, 100mM KCl, 2mM EDTA, 20% glycerol, 0.5mM DTT, 0.5mM PMSF, pH 7.5).

    RNA Binding Assay:

    Article Title: Exon 9 skipping of apoptotic caspase-2 pre-mRNA is promoted by SRp20 through interaction with exon 8
    Article Snippet: Paragraph title: 2.5. RNA binding assay of SRp20 ... Briefly, chemically synthesized 5’ biotin labeled wild type (UUCUUCAUCC) and mutant (UGAAGCGUCC) RNA were covalently linked with Streptavidin agarose conjugate (Millipore).

    Binding Assay:

    Article Title: hnRNP A1 contacts exon 5 to promote exon 6 inclusion of apoptotic Fas gene
    Article Snippet: Paragraph title: RNA-hnRNP A1 binding assay ... Biotinylated RNA was covalently linked with Streptavidin agarose conjugate (Millipore) by mixing together for 1 h in buffer D (20 mM Tris–Cl, 100 mM KCl, 2 mM EDTA, 20 % glycerol, 0.5 mM DTT, 0.5 mM PMSF, pH 7.5).

    SDS Page:

    Article Title: hnRNP A1 contacts exon 5 to promote exon 6 inclusion of apoptotic Fas gene
    Article Snippet: Biotinylated RNA was covalently linked with Streptavidin agarose conjugate (Millipore) by mixing together for 1 h in buffer D (20 mM Tris–Cl, 100 mM KCl, 2 mM EDTA, 20 % glycerol, 0.5 mM DTT, 0.5 mM PMSF, pH 7.5). .. Streptavidin agarose linked biotin-RNA was incubated with HeLa nuclear extract for 3.5 h at 4 °C in buffer D. After washing with buffer for five times, we added SDS–PAGE loading dye and then boiled the sample.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Millipore streptavidin conjugated agarose
    Cell surface localization of unglycosylated gp130 in NECs treated with tunicamycin. To confirm the cell surface localization of unglycosylated gp130, cell surface proteins expressed in NECs treated with or without tunicamycin (2 μg/ml) for 8 h were biotinylated by treating the cells with sulfo-NHS-LC-biotin. After lysis, biotinylated cell surface proteins were pulled down with <t>streptavidin-conjugated</t> agarose and then analyzed by Western-blot using anti-gp130 antibody ( A ) or anti-LIFR antibody ( B ).
    Streptavidin Conjugated Agarose, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin conjugated agarose/product/Millipore
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    streptavidin conjugated agarose - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    Image Search Results


    Cell surface localization of unglycosylated gp130 in NECs treated with tunicamycin. To confirm the cell surface localization of unglycosylated gp130, cell surface proteins expressed in NECs treated with or without tunicamycin (2 μg/ml) for 8 h were biotinylated by treating the cells with sulfo-NHS-LC-biotin. After lysis, biotinylated cell surface proteins were pulled down with streptavidin-conjugated agarose and then analyzed by Western-blot using anti-gp130 antibody ( A ) or anti-LIFR antibody ( B ).

    Journal: Biochemical and biophysical research communications

    Article Title: N-Glycans modulate the activation of gp130 in mouse embryonic neural precursor cells

    doi: 10.1016/j.bbrc.2009.05.132

    Figure Lengend Snippet: Cell surface localization of unglycosylated gp130 in NECs treated with tunicamycin. To confirm the cell surface localization of unglycosylated gp130, cell surface proteins expressed in NECs treated with or without tunicamycin (2 μg/ml) for 8 h were biotinylated by treating the cells with sulfo-NHS-LC-biotin. After lysis, biotinylated cell surface proteins were pulled down with streptavidin-conjugated agarose and then analyzed by Western-blot using anti-gp130 antibody ( A ) or anti-LIFR antibody ( B ).

    Article Snippet: Biotinylated cell surface proteins in the lysates were pulled down by gentle agitation in the presence of streptavidin-conjugated agarose (EMD Biosciences) at 4°C overnight, washed three times with the lysis buffer, denatured in Laemmli sample buffer by boiling and subjected to Western-blot analysis using an anti-gp130 antibody or an anti-LIFR antibody.

    Techniques: Lysis, Western Blot

    NEDD9 depletion does not affect internalization of MMP14 (a) Western blot (WB) analysis of immunoprecipitated (IP) biotinylated MMP14 from shCon-, shNEDD9 (N1, N2)-MDA-MB-231cells: no strip-0°C (lines 1-3), the rest incubated at 37°C for indicated times, striped with MESNA, lysed, IPed with streptavidin agarose and probed with anti-MMP14 antibody. (b) Quantification of WBs as in (a), three independent experiments, graphs are mean percent (%) of relative intensity units (RIU) to no strip conditions (100%) ±S.E.M; one-way ANOVA with Dunnett's post-hoc analysis *p= 0.0054, 0.0049, 0.0098, 0.0028 for 5, 15, 30 and 60 min respectively. (c) Representative confocal images of shCon-, shNEDD9 (N1, N2)-MDA-MB-231cells expressing PA-mCherry-MMP14 before photo-activation (pre-PA), after- (PA), and 5 min after PA in a defined area. Scale bar, 10μm; inserts are the enlarged areas of PA; BF-bright field image. (d) Quantification of relative fluorescence intensity units (RFU) of mCherry-MMP14 in cells as in (c) in perinuclear area (p); graph is mean RFU % of total RFU/cell ±S.E.M; 10 cells/per treatment (Con, N1, N2); F test performed for fitted lines, p is non-significant (ns) for shCon/shN1 or /N2. (e) Quantification of relative fluorescence intensity (RFU) of mCherry-MMP14 in cells as in (c) in PA area (black rectangle); graph is mean RFU (%) of max RFU/cell (100%) ±S.E.M; 10 cells/per treatment (Con, N1, N2), F test performed for fitted lines, p is (ns).

    Journal: Oncogene

    Article Title: NEDD9/Arf6-Dependent Endocytic Trafficking of Matrix Metalloproteinase 14: A Novel Mechanism for Blocking Mesenchymal Cell Invasion and Metastasis of Breast Cancer

    doi: 10.1038/onc.2014.297

    Figure Lengend Snippet: NEDD9 depletion does not affect internalization of MMP14 (a) Western blot (WB) analysis of immunoprecipitated (IP) biotinylated MMP14 from shCon-, shNEDD9 (N1, N2)-MDA-MB-231cells: no strip-0°C (lines 1-3), the rest incubated at 37°C for indicated times, striped with MESNA, lysed, IPed with streptavidin agarose and probed with anti-MMP14 antibody. (b) Quantification of WBs as in (a), three independent experiments, graphs are mean percent (%) of relative intensity units (RIU) to no strip conditions (100%) ±S.E.M; one-way ANOVA with Dunnett's post-hoc analysis *p= 0.0054, 0.0049, 0.0098, 0.0028 for 5, 15, 30 and 60 min respectively. (c) Representative confocal images of shCon-, shNEDD9 (N1, N2)-MDA-MB-231cells expressing PA-mCherry-MMP14 before photo-activation (pre-PA), after- (PA), and 5 min after PA in a defined area. Scale bar, 10μm; inserts are the enlarged areas of PA; BF-bright field image. (d) Quantification of relative fluorescence intensity units (RFU) of mCherry-MMP14 in cells as in (c) in perinuclear area (p); graph is mean RFU % of total RFU/cell ±S.E.M; 10 cells/per treatment (Con, N1, N2); F test performed for fitted lines, p is non-significant (ns) for shCon/shN1 or /N2. (e) Quantification of relative fluorescence intensity (RFU) of mCherry-MMP14 in cells as in (c) in PA area (black rectangle); graph is mean RFU (%) of max RFU/cell (100%) ±S.E.M; 10 cells/per treatment (Con, N1, N2), F test performed for fitted lines, p is (ns).

    Article Snippet: Cells were homogenized in PTY buffer , and biotinylated molecules were pulled down with streptavidin-conjugated agarose (Millipore) and used for western blotting.

    Techniques: Western Blot, Immunoprecipitation, Multiple Displacement Amplification, Stripping Membranes, Incubation, Expressing, Activation Assay, Fluorescence