streptavidin agarose column  (Millipore)


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    Structured Review

    Millipore streptavidin agarose column
    Streptavidin Agarose Column, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin agarose column/product/Millipore
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavidin agarose column - by Bioz Stars, 2020-04
    87/100 stars

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    Related Articles

    Transfection:

    Article Title: Extracellular complexes of the hematopoietic human and mouse CSF-1 receptor are driven by common assembly principles
    Article Snippet: Biotinylated thrombin was removed using a streptavidin-agarose column (Novagen). .. Recombinant glycosylated human and murine CSF-1R ectodomain variants were produced in transiently transfected HEK293T cells in the presence of kifunensine based on established protocols ( ; ).

    Positron Emission Tomography:

    Article Title: Dodine as a Protein Denaturant: The Best of Two Worlds?
    Article Snippet: Thrombin and protein were separated either by isolating biotinylated thrombin on a streptavidin agarose column (EMD Millipore, Billerica, MA) or via size exclusion filtration. .. For measurements, proteins were dissolved into 20 mM phosphate buffer, pH = 7.0–7.5 or into sterile MilliQ water (EMD Millipore, Billerica, MA). λ was expressed from a pET-15b (EMD Millipore, Billerica, MA) vector encoding an N-terminal hexahistidine tag and a thrombin recognition sequence.

    Filtration:

    Article Title: Dodine as a Protein Denaturant: The Best of Two Worlds?
    Article Snippet: .. Thrombin and protein were separated either by isolating biotinylated thrombin on a streptavidin agarose column (EMD Millipore, Billerica, MA) or via size exclusion filtration. ..

    Chromatography:

    Article Title: Extracellular complexes of the hematopoietic human and mouse CSF-1 receptor are driven by common assembly principles
    Article Snippet: Biotinylated thrombin was removed using a streptavidin-agarose column (Novagen). .. Thrombin-treated h/mCSF-1 were purified using a Source 30Q anion-exchange resin and followed by gel-filtration chromatography on a Superdex-75 column (GE Healthcare).

    Size-exclusion Chromatography:

    Article Title: Extracellular complexes of the hematopoietic human and mouse CSF-1 receptor are driven by common assembly principles
    Article Snippet: The clarified refolding mixture was loaded onto a HisTrap FF 5 mL affinity column, eluted and subsequently purified by size exclusion chromatography using a Prep-Grade HiLoad 16/60 Superdex 75 column (GE Healthcare). .. Biotinylated thrombin was removed using a streptavidin-agarose column (Novagen).

    Purification:

    Article Title: Extracellular complexes of the hematopoietic human and mouse CSF-1 receptor are driven by common assembly principles
    Article Snippet: The clarified refolding mixture was loaded onto a HisTrap FF 5 mL affinity column, eluted and subsequently purified by size exclusion chromatography using a Prep-Grade HiLoad 16/60 Superdex 75 column (GE Healthcare). .. Biotinylated thrombin was removed using a streptavidin-agarose column (Novagen).

    Article Title: Dodine as a Protein Denaturant: The Best of Two Worlds?
    Article Snippet: Paragraph title: Protein expression and purification ... Thrombin and protein were separated either by isolating biotinylated thrombin on a streptavidin agarose column (EMD Millipore, Billerica, MA) or via size exclusion filtration.

    Produced:

    Article Title: Extracellular complexes of the hematopoietic human and mouse CSF-1 receptor are driven by common assembly principles
    Article Snippet: Biotinylated thrombin was removed using a streptavidin-agarose column (Novagen). .. Recombinant glycosylated human and murine CSF-1R ectodomain variants were produced in transiently transfected HEK293T cells in the presence of kifunensine based on established protocols ( ; ).

    Incubation:

    Article Title: Extracellular complexes of the hematopoietic human and mouse CSF-1 receptor are driven by common assembly principles
    Article Snippet: To remove the N-terminal His-tag, h/mCSF-1 was subsequently incubated overnight at room temperature with one unit of biotinylated thrombin (Novagen) per milligram of h/mCSF-1. .. Biotinylated thrombin was removed using a streptavidin-agarose column (Novagen).

    Affinity Column:

    Article Title: Extracellular complexes of the hematopoietic human and mouse CSF-1 receptor are driven by common assembly principles
    Article Snippet: The clarified refolding mixture was loaded onto a HisTrap FF 5 mL affinity column, eluted and subsequently purified by size exclusion chromatography using a Prep-Grade HiLoad 16/60 Superdex 75 column (GE Healthcare). .. Biotinylated thrombin was removed using a streptavidin-agarose column (Novagen).

    Expressing:

    Article Title: Dodine as a Protein Denaturant: The Best of Two Worlds?
    Article Snippet: Paragraph title: Protein expression and purification ... Thrombin and protein were separated either by isolating biotinylated thrombin on a streptavidin agarose column (EMD Millipore, Billerica, MA) or via size exclusion filtration.

    Sonication:

    Article Title: Dodine as a Protein Denaturant: The Best of Two Worlds?
    Article Snippet: Cells were lysed via sonication and the soluble fraction was isolated by centrifugation at 10,000 rpm for 20 minutes. .. Thrombin and protein were separated either by isolating biotinylated thrombin on a streptavidin agarose column (EMD Millipore, Billerica, MA) or via size exclusion filtration.

    Sequencing:

    Article Title: Dodine as a Protein Denaturant: The Best of Two Worlds?
    Article Snippet: Thrombin and protein were separated either by isolating biotinylated thrombin on a streptavidin agarose column (EMD Millipore, Billerica, MA) or via size exclusion filtration. .. For measurements, proteins were dissolved into 20 mM phosphate buffer, pH = 7.0–7.5 or into sterile MilliQ water (EMD Millipore, Billerica, MA). λ was expressed from a pET-15b (EMD Millipore, Billerica, MA) vector encoding an N-terminal hexahistidine tag and a thrombin recognition sequence.

    Lysis:

    Article Title: Dodine as a Protein Denaturant: The Best of Two Worlds?
    Article Snippet: Cells were harvested by centrifugation at 5,000 rpm for 20 minutes and resuspended in lysis buffer. .. Thrombin and protein were separated either by isolating biotinylated thrombin on a streptavidin agarose column (EMD Millipore, Billerica, MA) or via size exclusion filtration.

    Centrifugation:

    Article Title: Dodine as a Protein Denaturant: The Best of Two Worlds?
    Article Snippet: Cells were lysed via sonication and the soluble fraction was isolated by centrifugation at 10,000 rpm for 20 minutes. .. Thrombin and protein were separated either by isolating biotinylated thrombin on a streptavidin agarose column (EMD Millipore, Billerica, MA) or via size exclusion filtration.

    Recombinant:

    Article Title: Extracellular complexes of the hematopoietic human and mouse CSF-1 receptor are driven by common assembly principles
    Article Snippet: Paragraph title: Production of recombinant human and mouse CSF-1, CSF-1R and CSF-1 ligand-receptor complexes ... Biotinylated thrombin was removed using a streptavidin-agarose column (Novagen).

    SDS Page:

    Article Title: Extracellular complexes of the hematopoietic human and mouse CSF-1 receptor are driven by common assembly principles
    Article Snippet: Proteolytic cleavage was monitored by SDS–PAGE. .. Biotinylated thrombin was removed using a streptavidin-agarose column (Novagen).

    Plasmid Preparation:

    Article Title: Dodine as a Protein Denaturant: The Best of Two Worlds?
    Article Snippet: Thrombin and protein were separated either by isolating biotinylated thrombin on a streptavidin agarose column (EMD Millipore, Billerica, MA) or via size exclusion filtration. .. For measurements, proteins were dissolved into 20 mM phosphate buffer, pH = 7.0–7.5 or into sterile MilliQ water (EMD Millipore, Billerica, MA). λ was expressed from a pET-15b (EMD Millipore, Billerica, MA) vector encoding an N-terminal hexahistidine tag and a thrombin recognition sequence.

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    Millipore tev protease
    Tev Protease, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tev protease/product/Millipore
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    Millipore streptavidin sepharose affinity columns
    <t>Streptavidin-affinity-enriched</t> PBI686-tagged protein extracts. Total protein extract, photo-cross-linked with PBI686 was enriched using <t>streptavidin—Sepharose</t> affinity chromatography. Eluted proteins were desalted, concentrated and analysed using far-Western blot analysis with a streptavidin—HRP conjugate (lane 1) and silver-staining (lane 2) techniques. Band regions that were excised are indicated with letters A-C. The molecular mass in kDa is indicated.
    Streptavidin Sepharose Affinity Columns, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin sepharose affinity columns/product/Millipore
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavidin sepharose affinity columns - by Bioz Stars, 2020-04
    88/100 stars
      Buy from Supplier

    87
    Millipore streptavidin agarose column
    <t>Streptavidin-affinity-enriched</t> PBI686-tagged protein extracts. Total protein extract, photo-cross-linked with PBI686 was enriched using <t>streptavidin—Sepharose</t> affinity chromatography. Eluted proteins were desalted, concentrated and analysed using far-Western blot analysis with a streptavidin—HRP conjugate (lane 1) and silver-staining (lane 2) techniques. Band regions that were excised are indicated with letters A-C. The molecular mass in kDa is indicated.
    Streptavidin Agarose Column, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin agarose column/product/Millipore
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavidin agarose column - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    Image Search Results


    Streptavidin-affinity-enriched PBI686-tagged protein extracts. Total protein extract, photo-cross-linked with PBI686 was enriched using streptavidin—Sepharose affinity chromatography. Eluted proteins were desalted, concentrated and analysed using far-Western blot analysis with a streptavidin—HRP conjugate (lane 1) and silver-staining (lane 2) techniques. Band regions that were excised are indicated with letters A-C. The molecular mass in kDa is indicated.

    Journal: PLoS ONE

    Article Title: Identification of Interactions between Abscisic Acid and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase

    doi: 10.1371/journal.pone.0133033

    Figure Lengend Snippet: Streptavidin-affinity-enriched PBI686-tagged protein extracts. Total protein extract, photo-cross-linked with PBI686 was enriched using streptavidin—Sepharose affinity chromatography. Eluted proteins were desalted, concentrated and analysed using far-Western blot analysis with a streptavidin—HRP conjugate (lane 1) and silver-staining (lane 2) techniques. Band regions that were excised are indicated with letters A-C. The molecular mass in kDa is indicated.

    Article Snippet: Protein fractions were eluted by streptavidin—Sepharose affinity columns, desalted, concentrated using AmiconTM Ultrafree centrifugal filters (Millipore), and visualized using a FOCUS-FAST silver-stain kit.

    Techniques: Affinity Chromatography, Far Western Blot, Silver Staining