Structured Review

Jackson Immuno streptavidin alexa fluor 647
(A) Schematic overview of the N -glycosylation site inside the putative CRD of DCIR. NES is the only predicted N -glycosylation site of DCIR. The EPS motif is the predicted glycan binding motif. Adapted from Bates et al . . (B) Increased binding to DCIR ligands in the presence of truncated or complete absence of glycans on the DCIR-Fc construct. Binding of wild-type and the glycosylation mutant DCIR-Fc to immobilized neoglycoconjugates was measured in a glycan binding assay. Average DCIR-Fc binding ± SEM was calculated from 4 independent experiments. (C) DCIR expression on DCIR transduced cell lines was determined by flow cytometry. Filled histogram represents isotype control and black line indicates the DCIR expression. (D) Binding of glycan beads to cellular DCIR could not be detected. Binding of fluorescent labeled glycan beads to the different DCIR expressing cell lines was measured by flow cytometry in the absence of serum. As a control binding of the fluorescent labeled glycan beads to CHO-DC-SIGN was measured. One representative experiment out of 3 is shown. (E) Glycan binding of PAA-glycans to cellular DCIR could not be detected. Binding of biotinylated PAA-glycans pre-incubated with streptavidin-Alexa <t>Fluor</t> <t>647</t> to the different DCIR and DC-SIGN expressing cell lines was measured by flow cytometry after 2 hours incubation at 37°C. Binding of biotinylated PAA-glycans to CHO-DC-SIGN served as a positive control. One representative experiment out of 3 is shown.
Streptavidin Alexa Fluor 647, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin alexa fluor 647/product/Jackson Immuno
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
streptavidin alexa fluor 647 - by Bioz Stars, 2023-02
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1) Product Images from "Ligand Binding and Signaling of Dendritic Cell Immunoreceptor (DCIR) Is Modulated by the Glycosylation of the Carbohydrate Recognition Domain"

Article Title: Ligand Binding and Signaling of Dendritic Cell Immunoreceptor (DCIR) Is Modulated by the Glycosylation of the Carbohydrate Recognition Domain

Journal: PLoS ONE

doi: 10.1371/journal.pone.0066266

(A) Schematic overview of the N -glycosylation site inside the putative CRD of DCIR. NES is the only predicted N -glycosylation site of DCIR. The EPS motif is the predicted glycan binding motif. Adapted from Bates et al . . (B) Increased binding to DCIR ligands in the presence of truncated or complete absence of glycans on the DCIR-Fc construct. Binding of wild-type and the glycosylation mutant DCIR-Fc to immobilized neoglycoconjugates was measured in a glycan binding assay. Average DCIR-Fc binding ± SEM was calculated from 4 independent experiments. (C) DCIR expression on DCIR transduced cell lines was determined by flow cytometry. Filled histogram represents isotype control and black line indicates the DCIR expression. (D) Binding of glycan beads to cellular DCIR could not be detected. Binding of fluorescent labeled glycan beads to the different DCIR expressing cell lines was measured by flow cytometry in the absence of serum. As a control binding of the fluorescent labeled glycan beads to CHO-DC-SIGN was measured. One representative experiment out of 3 is shown. (E) Glycan binding of PAA-glycans to cellular DCIR could not be detected. Binding of biotinylated PAA-glycans pre-incubated with streptavidin-Alexa Fluor 647 to the different DCIR and DC-SIGN expressing cell lines was measured by flow cytometry after 2 hours incubation at 37°C. Binding of biotinylated PAA-glycans to CHO-DC-SIGN served as a positive control. One representative experiment out of 3 is shown.
Figure Legend Snippet: (A) Schematic overview of the N -glycosylation site inside the putative CRD of DCIR. NES is the only predicted N -glycosylation site of DCIR. The EPS motif is the predicted glycan binding motif. Adapted from Bates et al . . (B) Increased binding to DCIR ligands in the presence of truncated or complete absence of glycans on the DCIR-Fc construct. Binding of wild-type and the glycosylation mutant DCIR-Fc to immobilized neoglycoconjugates was measured in a glycan binding assay. Average DCIR-Fc binding ± SEM was calculated from 4 independent experiments. (C) DCIR expression on DCIR transduced cell lines was determined by flow cytometry. Filled histogram represents isotype control and black line indicates the DCIR expression. (D) Binding of glycan beads to cellular DCIR could not be detected. Binding of fluorescent labeled glycan beads to the different DCIR expressing cell lines was measured by flow cytometry in the absence of serum. As a control binding of the fluorescent labeled glycan beads to CHO-DC-SIGN was measured. One representative experiment out of 3 is shown. (E) Glycan binding of PAA-glycans to cellular DCIR could not be detected. Binding of biotinylated PAA-glycans pre-incubated with streptavidin-Alexa Fluor 647 to the different DCIR and DC-SIGN expressing cell lines was measured by flow cytometry after 2 hours incubation at 37°C. Binding of biotinylated PAA-glycans to CHO-DC-SIGN served as a positive control. One representative experiment out of 3 is shown.

Techniques Used: Binding Assay, Construct, Mutagenesis, Expressing, Flow Cytometry, Labeling, Incubation, Positive Control


Structured Review

Jackson Immuno streptavidin alexa fluor 647
Streptavidin Alexa Fluor 647, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin alexa fluor 647/product/Jackson Immuno
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
streptavidin alexa fluor 647 - by Bioz Stars, 2023-02
86/100 stars

Images


Structured Review

Jackson Immuno streptavidin alexa 647
Streptavidin Alexa 647, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin alexa 647/product/Jackson Immuno
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
streptavidin alexa 647 - by Bioz Stars, 2023-02
86/100 stars

Images


Structured Review

Jackson Immuno streptavidin alexa fluor 647
Streptavidin Alexa Fluor 647, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin alexa fluor 647/product/Jackson Immuno
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
streptavidin alexa fluor 647 - by Bioz Stars, 2023-02
86/100 stars

Images


Structured Review

Jackson Immuno alexa fluor 647 streptavidin
Alexa Fluor 647 Streptavidin, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alexa fluor 647 streptavidin/product/Jackson Immuno
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
alexa fluor 647 streptavidin - by Bioz Stars, 2023-02
86/100 stars

Images


Structured Review

Jackson Immuno alexa fluor 647 streptavidin
Optimized working concentration and incubation time for antibodies used to label E14.5 FL cryo-sections
Alexa Fluor 647 Streptavidin, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alexa fluor 647 streptavidin/product/Jackson Immuno
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
alexa fluor 647 streptavidin - by Bioz Stars, 2023-02
86/100 stars

Images

1) Product Images from "Immuno-localization of definitive hematopoietic stem cells in the vascular niche of mouse fetal liver"

Article Title: Immuno-localization of definitive hematopoietic stem cells in the vascular niche of mouse fetal liver

Journal: STAR Protocols

doi: 10.1016/j.xpro.2022.101580

Optimized working concentration and incubation time for antibodies used to label E14.5 FL cryo-sections
Figure Legend Snippet: Optimized working concentration and incubation time for antibodies used to label E14.5 FL cryo-sections

Techniques Used: Concentration Assay, Incubation

Sequence of addition for performing HSC CD31 combination experiment in E14.5 FL tissue section
Figure Legend Snippet: Sequence of addition for performing HSC CD31 combination experiment in E14.5 FL tissue section

Techniques Used: Sequencing, Incubation, Purification


Figure Legend Snippet:

Techniques Used: Recombinant, Avidin-Biotin Assay, Cell Culture, Microscopy, Software


Structured Review

Jackson Immuno streptavidin alexa fluor 647
Streptavidin Alexa Fluor 647, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin alexa fluor 647/product/Jackson Immuno
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
streptavidin alexa fluor 647 - by Bioz Stars, 2023-02
86/100 stars

Images


Structured Review

Jackson Immuno streptavidin 647
Streptavidin 647, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin 647/product/Jackson Immuno
Average 98 stars, based on 1 article reviews
Price from $9.99 to $1999.99
streptavidin 647 - by Bioz Stars, 2023-02
98/100 stars

Images


Structured Review

Jackson Immuno streptavidin 647
Streptavidin 647, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin 647/product/Jackson Immuno
Average 98 stars, based on 1 article reviews
Price from $9.99 to $1999.99
streptavidin 647 - by Bioz Stars, 2023-02
98/100 stars

Images


Structured Review

Jackson Immuno streptavidin alexa fluor 647
Secondary antibodies and dilutions used in the study
Streptavidin Alexa Fluor 647, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin alexa fluor 647/product/Jackson Immuno
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
streptavidin alexa fluor 647 - by Bioz Stars, 2023-02
86/100 stars

Images

1) Product Images from "New Subregions of the Mouse Entopeduncular Nucleus Defined by the Complementary Immunoreactivities for Substance P and Cannabinoid Type-1 Receptor Combined with Distributions of Different Neuronal Types"

Article Title: New Subregions of the Mouse Entopeduncular Nucleus Defined by the Complementary Immunoreactivities for Substance P and Cannabinoid Type-1 Receptor Combined with Distributions of Different Neuronal Types

Journal: eNeuro

doi: 10.1523/ENEURO.0208-22.2022

Secondary antibodies and dilutions used in the study
Figure Legend Snippet: Secondary antibodies and dilutions used in the study

Techniques Used:

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    Jackson Immuno streptavidin alexa fluor 647
    (A) Schematic overview of the N -glycosylation site inside the putative CRD of DCIR. NES is the only predicted N -glycosylation site of DCIR. The EPS motif is the predicted glycan binding motif. Adapted from Bates et al . . (B) Increased binding to DCIR ligands in the presence of truncated or complete absence of glycans on the DCIR-Fc construct. Binding of wild-type and the glycosylation mutant DCIR-Fc to immobilized neoglycoconjugates was measured in a glycan binding assay. Average DCIR-Fc binding ± SEM was calculated from 4 independent experiments. (C) DCIR expression on DCIR transduced cell lines was determined by flow cytometry. Filled histogram represents isotype control and black line indicates the DCIR expression. (D) Binding of glycan beads to cellular DCIR could not be detected. Binding of fluorescent labeled glycan beads to the different DCIR expressing cell lines was measured by flow cytometry in the absence of serum. As a control binding of the fluorescent labeled glycan beads to CHO-DC-SIGN was measured. One representative experiment out of 3 is shown. (E) Glycan binding of PAA-glycans to cellular DCIR could not be detected. Binding of biotinylated PAA-glycans pre-incubated with streptavidin-Alexa <t>Fluor</t> <t>647</t> to the different DCIR and DC-SIGN expressing cell lines was measured by flow cytometry after 2 hours incubation at 37°C. Binding of biotinylated PAA-glycans to CHO-DC-SIGN served as a positive control. One representative experiment out of 3 is shown.
    Streptavidin Alexa Fluor 647, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin alexa fluor 647/product/Jackson Immuno
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavidin alexa fluor 647 - by Bioz Stars, 2023-02
    86/100 stars
      Buy from Supplier

    86
    Jackson Immuno streptavidin alexa 647
    (A) Schematic overview of the N -glycosylation site inside the putative CRD of DCIR. NES is the only predicted N -glycosylation site of DCIR. The EPS motif is the predicted glycan binding motif. Adapted from Bates et al . . (B) Increased binding to DCIR ligands in the presence of truncated or complete absence of glycans on the DCIR-Fc construct. Binding of wild-type and the glycosylation mutant DCIR-Fc to immobilized neoglycoconjugates was measured in a glycan binding assay. Average DCIR-Fc binding ± SEM was calculated from 4 independent experiments. (C) DCIR expression on DCIR transduced cell lines was determined by flow cytometry. Filled histogram represents isotype control and black line indicates the DCIR expression. (D) Binding of glycan beads to cellular DCIR could not be detected. Binding of fluorescent labeled glycan beads to the different DCIR expressing cell lines was measured by flow cytometry in the absence of serum. As a control binding of the fluorescent labeled glycan beads to CHO-DC-SIGN was measured. One representative experiment out of 3 is shown. (E) Glycan binding of PAA-glycans to cellular DCIR could not be detected. Binding of biotinylated PAA-glycans pre-incubated with streptavidin-Alexa <t>Fluor</t> <t>647</t> to the different DCIR and DC-SIGN expressing cell lines was measured by flow cytometry after 2 hours incubation at 37°C. Binding of biotinylated PAA-glycans to CHO-DC-SIGN served as a positive control. One representative experiment out of 3 is shown.
    Streptavidin Alexa 647, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin alexa 647/product/Jackson Immuno
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavidin alexa 647 - by Bioz Stars, 2023-02
    86/100 stars
      Buy from Supplier

    86
    Jackson Immuno alexa fluor 647 streptavidin
    (A) Schematic overview of the N -glycosylation site inside the putative CRD of DCIR. NES is the only predicted N -glycosylation site of DCIR. The EPS motif is the predicted glycan binding motif. Adapted from Bates et al . . (B) Increased binding to DCIR ligands in the presence of truncated or complete absence of glycans on the DCIR-Fc construct. Binding of wild-type and the glycosylation mutant DCIR-Fc to immobilized neoglycoconjugates was measured in a glycan binding assay. Average DCIR-Fc binding ± SEM was calculated from 4 independent experiments. (C) DCIR expression on DCIR transduced cell lines was determined by flow cytometry. Filled histogram represents isotype control and black line indicates the DCIR expression. (D) Binding of glycan beads to cellular DCIR could not be detected. Binding of fluorescent labeled glycan beads to the different DCIR expressing cell lines was measured by flow cytometry in the absence of serum. As a control binding of the fluorescent labeled glycan beads to CHO-DC-SIGN was measured. One representative experiment out of 3 is shown. (E) Glycan binding of PAA-glycans to cellular DCIR could not be detected. Binding of biotinylated PAA-glycans pre-incubated with streptavidin-Alexa <t>Fluor</t> <t>647</t> to the different DCIR and DC-SIGN expressing cell lines was measured by flow cytometry after 2 hours incubation at 37°C. Binding of biotinylated PAA-glycans to CHO-DC-SIGN served as a positive control. One representative experiment out of 3 is shown.
    Alexa Fluor 647 Streptavidin, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 647 streptavidin/product/Jackson Immuno
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 647 streptavidin - by Bioz Stars, 2023-02
    86/100 stars
      Buy from Supplier

    98
    Jackson Immuno streptavidin 647
    (A) Schematic overview of the N -glycosylation site inside the putative CRD of DCIR. NES is the only predicted N -glycosylation site of DCIR. The EPS motif is the predicted glycan binding motif. Adapted from Bates et al . . (B) Increased binding to DCIR ligands in the presence of truncated or complete absence of glycans on the DCIR-Fc construct. Binding of wild-type and the glycosylation mutant DCIR-Fc to immobilized neoglycoconjugates was measured in a glycan binding assay. Average DCIR-Fc binding ± SEM was calculated from 4 independent experiments. (C) DCIR expression on DCIR transduced cell lines was determined by flow cytometry. Filled histogram represents isotype control and black line indicates the DCIR expression. (D) Binding of glycan beads to cellular DCIR could not be detected. Binding of fluorescent labeled glycan beads to the different DCIR expressing cell lines was measured by flow cytometry in the absence of serum. As a control binding of the fluorescent labeled glycan beads to CHO-DC-SIGN was measured. One representative experiment out of 3 is shown. (E) Glycan binding of PAA-glycans to cellular DCIR could not be detected. Binding of biotinylated PAA-glycans pre-incubated with streptavidin-Alexa <t>Fluor</t> <t>647</t> to the different DCIR and DC-SIGN expressing cell lines was measured by flow cytometry after 2 hours incubation at 37°C. Binding of biotinylated PAA-glycans to CHO-DC-SIGN served as a positive control. One representative experiment out of 3 is shown.
    Streptavidin 647, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin 647/product/Jackson Immuno
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavidin 647 - by Bioz Stars, 2023-02
    98/100 stars
      Buy from Supplier

    Image Search Results


    (A) Schematic overview of the N -glycosylation site inside the putative CRD of DCIR. NES is the only predicted N -glycosylation site of DCIR. The EPS motif is the predicted glycan binding motif. Adapted from Bates et al . . (B) Increased binding to DCIR ligands in the presence of truncated or complete absence of glycans on the DCIR-Fc construct. Binding of wild-type and the glycosylation mutant DCIR-Fc to immobilized neoglycoconjugates was measured in a glycan binding assay. Average DCIR-Fc binding ± SEM was calculated from 4 independent experiments. (C) DCIR expression on DCIR transduced cell lines was determined by flow cytometry. Filled histogram represents isotype control and black line indicates the DCIR expression. (D) Binding of glycan beads to cellular DCIR could not be detected. Binding of fluorescent labeled glycan beads to the different DCIR expressing cell lines was measured by flow cytometry in the absence of serum. As a control binding of the fluorescent labeled glycan beads to CHO-DC-SIGN was measured. One representative experiment out of 3 is shown. (E) Glycan binding of PAA-glycans to cellular DCIR could not be detected. Binding of biotinylated PAA-glycans pre-incubated with streptavidin-Alexa Fluor 647 to the different DCIR and DC-SIGN expressing cell lines was measured by flow cytometry after 2 hours incubation at 37°C. Binding of biotinylated PAA-glycans to CHO-DC-SIGN served as a positive control. One representative experiment out of 3 is shown.

    Journal: PLoS ONE

    Article Title: Ligand Binding and Signaling of Dendritic Cell Immunoreceptor (DCIR) Is Modulated by the Glycosylation of the Carbohydrate Recognition Domain

    doi: 10.1371/journal.pone.0066266

    Figure Lengend Snippet: (A) Schematic overview of the N -glycosylation site inside the putative CRD of DCIR. NES is the only predicted N -glycosylation site of DCIR. The EPS motif is the predicted glycan binding motif. Adapted from Bates et al . . (B) Increased binding to DCIR ligands in the presence of truncated or complete absence of glycans on the DCIR-Fc construct. Binding of wild-type and the glycosylation mutant DCIR-Fc to immobilized neoglycoconjugates was measured in a glycan binding assay. Average DCIR-Fc binding ± SEM was calculated from 4 independent experiments. (C) DCIR expression on DCIR transduced cell lines was determined by flow cytometry. Filled histogram represents isotype control and black line indicates the DCIR expression. (D) Binding of glycan beads to cellular DCIR could not be detected. Binding of fluorescent labeled glycan beads to the different DCIR expressing cell lines was measured by flow cytometry in the absence of serum. As a control binding of the fluorescent labeled glycan beads to CHO-DC-SIGN was measured. One representative experiment out of 3 is shown. (E) Glycan binding of PAA-glycans to cellular DCIR could not be detected. Binding of biotinylated PAA-glycans pre-incubated with streptavidin-Alexa Fluor 647 to the different DCIR and DC-SIGN expressing cell lines was measured by flow cytometry after 2 hours incubation at 37°C. Binding of biotinylated PAA-glycans to CHO-DC-SIGN served as a positive control. One representative experiment out of 3 is shown.

    Article Snippet: Antibodies used are α-DCIR 111F8.04 (Dendritics), α-DC-SIGN AZN-D1 , α-Langerin 10E2 , α-phosporylated tyrosine PY20 (Millipore), goat anti-mouse IgG Alexa Fluor 488 F(ab′) 2 fragment (Invitrogen), goat-anti-human Fc-PO (Jackson Immunoresearch), strepatavidin-PO (Invitrogen), goat-anti-human Fc (Jackson), goat-anti-human Fc-biotin (Jackson), streptavidin-Alexa Fluor 647 and 488 (Invitrogen) and IRDye goat-α-mouse IgG 800CW (Li-Cor).

    Techniques: Binding Assay, Construct, Mutagenesis, Expressing, Flow Cytometry, Labeling, Incubation, Positive Control