streptavadin horseradish peroxidase  (Vector Laboratories)


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    VECTASTAIN Elite ABC HRP Reagent R T U Peroxidase Ready to Use
    Description:
    VECTASTAIN Elite ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes VECTASTAIN Elite ABC SystemAdvanced avidin biotin technology The Elite ABC complex is smaller very uniform and highly active allowing more accessibility for binding to a biotinylated targetHighest sensitivity low background The VECTASTAIN Elite ABC system is the most sensitive avidin biotin complex based peroxidase system The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background Cost effective Higher sensitivity leads to lower cost per slide Available without Standard kit or with biotinylated species specific or universal secondary antibodies Available in Ready To Use R T U formats Prediluted stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Staining Kit reagents VECTASTAIN Elite ABC Kit Component The R T U VECTASTAIN Elite ABC reagent consists of 50 ml stabilized prediluted and preformed Elite ABC reagent The R T U kits are ideal for manual or automated tissue staining systems The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    Catalog Number:
    PK-7100
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Reactivity:
    No antibody included
    Size:
    50 ml
    Buy from Supplier


    Structured Review

    Vector Laboratories streptavadin horseradish peroxidase
    VECTASTAIN Elite ABC HRP Reagent R T U Peroxidase Ready to Use
    VECTASTAIN Elite ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes VECTASTAIN Elite ABC SystemAdvanced avidin biotin technology The Elite ABC complex is smaller very uniform and highly active allowing more accessibility for binding to a biotinylated targetHighest sensitivity low background The VECTASTAIN Elite ABC system is the most sensitive avidin biotin complex based peroxidase system The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background Cost effective Higher sensitivity leads to lower cost per slide Available without Standard kit or with biotinylated species specific or universal secondary antibodies Available in Ready To Use R T U formats Prediluted stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Staining Kit reagents VECTASTAIN Elite ABC Kit Component The R T U VECTASTAIN Elite ABC reagent consists of 50 ml stabilized prediluted and preformed Elite ABC reagent The R T U kits are ideal for manual or automated tissue staining systems The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    https://www.bioz.com/result/streptavadin horseradish peroxidase/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavadin horseradish peroxidase - by Bioz Stars, 2021-07
    99/100 stars

    Images

    1) Product Images from "Antibodies Targeted to the Brain with Image-Guided Focused Ultrasound Reduces Amyloid-? Plaque Load in the TgCRND8 Mouse Model of Alzheimer's Disease"

    Article Title: Antibodies Targeted to the Brain with Image-Guided Focused Ultrasound Reduces Amyloid-? Plaque Load in the TgCRND8 Mouse Model of Alzheimer's Disease

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0010549

    Focused ultrasound delivery of BAM-10 to the brain reduces Aβ plaque pathology in TgCRND8 mice. Coronal sections were stained with streptavadin-Cy2 for ( A ) biotinylated BAM-10 and ( B ) anti-Aβ antibody 6F3D for plaques to demonstrate that 6F3D binds to plaques which are strongly (arrows) and weakly (arrowheads) positive for BAM-10 ( C ). Once it was confirmed that BAM-10 does not interfere with 6F3D plaque detection, sections for mice in each treatment group were stained with 6F3D and the stereology software was used to draw contours outlining the FUS-targeted region (determined from MRI post-treatment scans) on the right side of the brain and an equivalent region on the contralateral side ( D ). Plaques were counted and measured at high magnification ( E ), using stereological methods. In 4 days, the mean ( F ) count, ( G ) size and ( H ) surface area of Aβ plaques on the right, MRIgFUS-targeted side of the brain was consistently reduced in comparison to the left side of the brain only for the BAM-10/FUS-treated mice ( F–H ; n = 6, paired t-tests, p = 0.008, p = 0.048 and p = 0.003, respectively). This difference is unique to BAM-10/FUS treatment as there was no significant difference between right and left side of the brain in mice from other treatment groups ( F′–H′ : BAM-10 treated group, n = 6, paired t-tests, p = 0.294, p = 0.941 and p = 0.402; F″–H″ : Untreated group, n = 6, paired t-tests, p = 0.502, p = 0.690, p = 0.610). Scale bars: A–C = 100 µm (inset = 20 µm); D = 1 mm; E = 50 µm.
    Figure Legend Snippet: Focused ultrasound delivery of BAM-10 to the brain reduces Aβ plaque pathology in TgCRND8 mice. Coronal sections were stained with streptavadin-Cy2 for ( A ) biotinylated BAM-10 and ( B ) anti-Aβ antibody 6F3D for plaques to demonstrate that 6F3D binds to plaques which are strongly (arrows) and weakly (arrowheads) positive for BAM-10 ( C ). Once it was confirmed that BAM-10 does not interfere with 6F3D plaque detection, sections for mice in each treatment group were stained with 6F3D and the stereology software was used to draw contours outlining the FUS-targeted region (determined from MRI post-treatment scans) on the right side of the brain and an equivalent region on the contralateral side ( D ). Plaques were counted and measured at high magnification ( E ), using stereological methods. In 4 days, the mean ( F ) count, ( G ) size and ( H ) surface area of Aβ plaques on the right, MRIgFUS-targeted side of the brain was consistently reduced in comparison to the left side of the brain only for the BAM-10/FUS-treated mice ( F–H ; n = 6, paired t-tests, p = 0.008, p = 0.048 and p = 0.003, respectively). This difference is unique to BAM-10/FUS treatment as there was no significant difference between right and left side of the brain in mice from other treatment groups ( F′–H′ : BAM-10 treated group, n = 6, paired t-tests, p = 0.294, p = 0.941 and p = 0.402; F″–H″ : Untreated group, n = 6, paired t-tests, p = 0.502, p = 0.690, p = 0.610). Scale bars: A–C = 100 µm (inset = 20 µm); D = 1 mm; E = 50 µm.

    Techniques Used: Mouse Assay, Staining, Software, Magnetic Resonance Imaging

    2) Product Images from "Antibodies Targeted to the Brain with Image-Guided Focused Ultrasound Reduces Amyloid-? Plaque Load in the TgCRND8 Mouse Model of Alzheimer's Disease"

    Article Title: Antibodies Targeted to the Brain with Image-Guided Focused Ultrasound Reduces Amyloid-? Plaque Load in the TgCRND8 Mouse Model of Alzheimer's Disease

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0010549

    Focused ultrasound delivery of BAM-10 to the brain reduces Aβ plaque pathology in TgCRND8 mice. Coronal sections were stained with streptavadin-Cy2 for ( A ) biotinylated BAM-10 and ( B ) anti-Aβ antibody 6F3D for plaques to demonstrate that 6F3D binds to plaques which are strongly (arrows) and weakly (arrowheads) positive for BAM-10 ( C ). Once it was confirmed that BAM-10 does not interfere with 6F3D plaque detection, sections for mice in each treatment group were stained with 6F3D and the stereology software was used to draw contours outlining the FUS-targeted region (determined from MRI post-treatment scans) on the right side of the brain and an equivalent region on the contralateral side ( D ). Plaques were counted and measured at high magnification ( E ), using stereological methods. In 4 days, the mean ( F ) count, ( G ) size and ( H ) surface area of Aβ plaques on the right, MRIgFUS-targeted side of the brain was consistently reduced in comparison to the left side of the brain only for the BAM-10/FUS-treated mice ( F–H ; n = 6, paired t-tests, p = 0.008, p = 0.048 and p = 0.003, respectively). This difference is unique to BAM-10/FUS treatment as there was no significant difference between right and left side of the brain in mice from other treatment groups ( F′–H′ : BAM-10 treated group, n = 6, paired t-tests, p = 0.294, p = 0.941 and p = 0.402; F″–H″ : Untreated group, n = 6, paired t-tests, p = 0.502, p = 0.690, p = 0.610). Scale bars: A–C = 100 µm (inset = 20 µm); D = 1 mm; E = 50 µm.
    Figure Legend Snippet: Focused ultrasound delivery of BAM-10 to the brain reduces Aβ plaque pathology in TgCRND8 mice. Coronal sections were stained with streptavadin-Cy2 for ( A ) biotinylated BAM-10 and ( B ) anti-Aβ antibody 6F3D for plaques to demonstrate that 6F3D binds to plaques which are strongly (arrows) and weakly (arrowheads) positive for BAM-10 ( C ). Once it was confirmed that BAM-10 does not interfere with 6F3D plaque detection, sections for mice in each treatment group were stained with 6F3D and the stereology software was used to draw contours outlining the FUS-targeted region (determined from MRI post-treatment scans) on the right side of the brain and an equivalent region on the contralateral side ( D ). Plaques were counted and measured at high magnification ( E ), using stereological methods. In 4 days, the mean ( F ) count, ( G ) size and ( H ) surface area of Aβ plaques on the right, MRIgFUS-targeted side of the brain was consistently reduced in comparison to the left side of the brain only for the BAM-10/FUS-treated mice ( F–H ; n = 6, paired t-tests, p = 0.008, p = 0.048 and p = 0.003, respectively). This difference is unique to BAM-10/FUS treatment as there was no significant difference between right and left side of the brain in mice from other treatment groups ( F′–H′ : BAM-10 treated group, n = 6, paired t-tests, p = 0.294, p = 0.941 and p = 0.402; F″–H″ : Untreated group, n = 6, paired t-tests, p = 0.502, p = 0.690, p = 0.610). Scale bars: A–C = 100 µm (inset = 20 µm); D = 1 mm; E = 50 µm.

    Techniques Used: Mouse Assay, Staining, Software, Magnetic Resonance Imaging

    Related Articles

    Amplification:

    Article Title: Diversification of heart progenitor cells by EGF signaling and differential modulation of ETS protein activity
    Article Snippet: Embryo fixations, immunostainings for proteins and RNA in situ hybridizations were carried out essentially as described ( ; ), except for stainings with anti-dpMAPK, for which the formaldehyde concentration was doubled and embryos were rehydrated from methanol and stained immediately after fixation. .. VectaStain Elite ABC-HRP kit (Vector Laboratories) and tyramide signal amplification (TSA, PerkinElmer Inc.) were used for detection of RNA and certain antigens (as indicated). ..

    Incubation:

    Article Title: MUC16/CA125 in the Context of Modular Proteins with an Annotated Role in Adhesion-Related Processes: In Silico Analysis
    Article Snippet: After incubation for 3 h at room temperature (RT), the wells were washed three times with 0.1 M PBS, pH 7.2 and biotinylated goat anti-mouse IgG (Vector Laboratories, Burlinghame, CA, USA) was added. .. Subsequent to incubation for 1 h, the wells were rinsed and Vectastain Elite ABC reagent (Vector Laboratories, Burlinghame, CA, USA) was added followed by incubation for 30 min. After another washing step, addition of TMB substrate solution and incubation for 10 min, the reaction was stopped with 0.16 M H2 SO4 . ..

    Immunohistochemistry:

    Article Title: Versican V2 Assembles the Extracellular Matrix Surrounding the Nodes of Ranvier in the CNS
    Article Snippet: .. Immunohistochemistry followed the avidin-biotinylated peroxidase method (VECTASTAIN Elite ABC Reagent, Vector Laboratories) using biotinylated secondary antibodies from Jackson ImmunoResearch Laboratories [biotin-SP-conjugated donkey anti-goat, guinea pig, or rabbit IgG (H+L)]. ..

    Article Title: Caerulein-induced acute pancreatitis in mice that constitutively overexpress Reg/PAP genes
    Article Snippet: An ELISA for Reg3α was then established using antibodies generated in rabbits and guinea pigs as described previously [ , ]. .. Paraffin sections (5 μm) of pancreas were processed for immunohistochemistry of Reg3α using the Vectastain ABC technique (Vector Labs, Burlingame, CA, USA). ..

    Article Title: SAD-B modulates epileptic seizure by regulating AMPA receptors in patients with temporal lobe epilepsy and in the PTZ-induced epileptic model
    Article Snippet: Minor modifications included the sectioning of the tissue into 30-µm thick sections for double immunofluorescence labelling. .. Immunohistochemistry Avidin-biotin-peroxidase complex (Vectastain Elite ABC; Vector Laboratories International, USA) was used for immunohistochemistry as described in our previous study ( ). .. Minor modifications included the use of a polyclonal mouse SAD-B antibody (diluted 1:100, Cat. No. ab206298; Abcam, UK) as the primary antibody.

    Avidin-Biotin Assay:

    Article Title: Versican V2 Assembles the Extracellular Matrix Surrounding the Nodes of Ranvier in the CNS
    Article Snippet: .. Immunohistochemistry followed the avidin-biotinylated peroxidase method (VECTASTAIN Elite ABC Reagent, Vector Laboratories) using biotinylated secondary antibodies from Jackson ImmunoResearch Laboratories [biotin-SP-conjugated donkey anti-goat, guinea pig, or rabbit IgG (H+L)]. ..

    Article Title: Innervation of Ventricular and Periventricular Brain Compartments
    Article Snippet: This was followed by overnight primary antibody incubation with 1.5% normal donkey serum and 0.3% Triton-X 100 on a rotator at room temperature. .. The next day, biotinylated anti-goat secondary antibody (Jackson) was used at final dilutions of 1:1000 in the same diluent (90–120 minutes on rotator at 24° C) and was followed by Vectastain Elite avidin-biotin reagents (Vector Labs, Burlingame, CA) with 0.3% Triton-X (90–120 minutes on rotator at 24° C). ..

    Article Title: SAD-B modulates epileptic seizure by regulating AMPA receptors in patients with temporal lobe epilepsy and in the PTZ-induced epileptic model
    Article Snippet: Minor modifications included the sectioning of the tissue into 30-µm thick sections for double immunofluorescence labelling. .. Immunohistochemistry Avidin-biotin-peroxidase complex (Vectastain Elite ABC; Vector Laboratories International, USA) was used for immunohistochemistry as described in our previous study ( ). .. Minor modifications included the use of a polyclonal mouse SAD-B antibody (diluted 1:100, Cat. No. ab206298; Abcam, UK) as the primary antibody.

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  • 99
    Vector Laboratories streptavadin horseradish peroxidase
    Focused ultrasound delivery of BAM-10 to the brain reduces Aβ plaque pathology in TgCRND8 mice. Coronal sections were stained with <t>streptavadin-Cy2</t> for ( A ) biotinylated BAM-10 and ( B ) anti-Aβ antibody 6F3D for plaques to demonstrate that 6F3D binds to plaques which are strongly (arrows) and weakly (arrowheads) positive for BAM-10 ( C ). Once it was confirmed that BAM-10 does not interfere with 6F3D plaque detection, sections for mice in each treatment group were stained with 6F3D and the stereology software was used to draw contours outlining the FUS-targeted region (determined from MRI post-treatment scans) on the right side of the brain and an equivalent region on the contralateral side ( D ). Plaques were counted and measured at high magnification ( E ), using stereological methods. In 4 days, the mean ( F ) count, ( G ) size and ( H ) surface area of Aβ plaques on the right, MRIgFUS-targeted side of the brain was consistently reduced in comparison to the left side of the brain only for the BAM-10/FUS-treated mice ( F–H ; n = 6, paired t-tests, p = 0.008, p = 0.048 and p = 0.003, respectively). This difference is unique to BAM-10/FUS treatment as there was no significant difference between right and left side of the brain in mice from other treatment groups ( F′–H′ : BAM-10 treated group, n = 6, paired t-tests, p = 0.294, p = 0.941 and p = 0.402; F″–H″ : Untreated group, n = 6, paired t-tests, p = 0.502, p = 0.690, p = 0.610). Scale bars: A–C = 100 µm (inset = 20 µm); D = 1 mm; E = 50 µm.
    Streptavadin Horseradish Peroxidase, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavadin horseradish peroxidase/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavadin horseradish peroxidase - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier

    86
    Vector Laboratories streptavadin hrp
    Focused ultrasound delivery of BAM-10 to the brain reduces Aβ plaque pathology in TgCRND8 mice. Coronal sections were stained with <t>streptavadin-Cy2</t> for ( A ) biotinylated BAM-10 and ( B ) anti-Aβ antibody 6F3D for plaques to demonstrate that 6F3D binds to plaques which are strongly (arrows) and weakly (arrowheads) positive for BAM-10 ( C ). Once it was confirmed that BAM-10 does not interfere with 6F3D plaque detection, sections for mice in each treatment group were stained with 6F3D and the stereology software was used to draw contours outlining the FUS-targeted region (determined from MRI post-treatment scans) on the right side of the brain and an equivalent region on the contralateral side ( D ). Plaques were counted and measured at high magnification ( E ), using stereological methods. In 4 days, the mean ( F ) count, ( G ) size and ( H ) surface area of Aβ plaques on the right, MRIgFUS-targeted side of the brain was consistently reduced in comparison to the left side of the brain only for the BAM-10/FUS-treated mice ( F–H ; n = 6, paired t-tests, p = 0.008, p = 0.048 and p = 0.003, respectively). This difference is unique to BAM-10/FUS treatment as there was no significant difference between right and left side of the brain in mice from other treatment groups ( F′–H′ : BAM-10 treated group, n = 6, paired t-tests, p = 0.294, p = 0.941 and p = 0.402; F″–H″ : Untreated group, n = 6, paired t-tests, p = 0.502, p = 0.690, p = 0.610). Scale bars: A–C = 100 µm (inset = 20 µm); D = 1 mm; E = 50 µm.
    Streptavadin Hrp, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavadin hrp/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavadin hrp - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Focused ultrasound delivery of BAM-10 to the brain reduces Aβ plaque pathology in TgCRND8 mice. Coronal sections were stained with streptavadin-Cy2 for ( A ) biotinylated BAM-10 and ( B ) anti-Aβ antibody 6F3D for plaques to demonstrate that 6F3D binds to plaques which are strongly (arrows) and weakly (arrowheads) positive for BAM-10 ( C ). Once it was confirmed that BAM-10 does not interfere with 6F3D plaque detection, sections for mice in each treatment group were stained with 6F3D and the stereology software was used to draw contours outlining the FUS-targeted region (determined from MRI post-treatment scans) on the right side of the brain and an equivalent region on the contralateral side ( D ). Plaques were counted and measured at high magnification ( E ), using stereological methods. In 4 days, the mean ( F ) count, ( G ) size and ( H ) surface area of Aβ plaques on the right, MRIgFUS-targeted side of the brain was consistently reduced in comparison to the left side of the brain only for the BAM-10/FUS-treated mice ( F–H ; n = 6, paired t-tests, p = 0.008, p = 0.048 and p = 0.003, respectively). This difference is unique to BAM-10/FUS treatment as there was no significant difference between right and left side of the brain in mice from other treatment groups ( F′–H′ : BAM-10 treated group, n = 6, paired t-tests, p = 0.294, p = 0.941 and p = 0.402; F″–H″ : Untreated group, n = 6, paired t-tests, p = 0.502, p = 0.690, p = 0.610). Scale bars: A–C = 100 µm (inset = 20 µm); D = 1 mm; E = 50 µm.

    Journal: PLoS ONE

    Article Title: Antibodies Targeted to the Brain with Image-Guided Focused Ultrasound Reduces Amyloid-? Plaque Load in the TgCRND8 Mouse Model of Alzheimer's Disease

    doi: 10.1371/journal.pone.0010549

    Figure Lengend Snippet: Focused ultrasound delivery of BAM-10 to the brain reduces Aβ plaque pathology in TgCRND8 mice. Coronal sections were stained with streptavadin-Cy2 for ( A ) biotinylated BAM-10 and ( B ) anti-Aβ antibody 6F3D for plaques to demonstrate that 6F3D binds to plaques which are strongly (arrows) and weakly (arrowheads) positive for BAM-10 ( C ). Once it was confirmed that BAM-10 does not interfere with 6F3D plaque detection, sections for mice in each treatment group were stained with 6F3D and the stereology software was used to draw contours outlining the FUS-targeted region (determined from MRI post-treatment scans) on the right side of the brain and an equivalent region on the contralateral side ( D ). Plaques were counted and measured at high magnification ( E ), using stereological methods. In 4 days, the mean ( F ) count, ( G ) size and ( H ) surface area of Aβ plaques on the right, MRIgFUS-targeted side of the brain was consistently reduced in comparison to the left side of the brain only for the BAM-10/FUS-treated mice ( F–H ; n = 6, paired t-tests, p = 0.008, p = 0.048 and p = 0.003, respectively). This difference is unique to BAM-10/FUS treatment as there was no significant difference between right and left side of the brain in mice from other treatment groups ( F′–H′ : BAM-10 treated group, n = 6, paired t-tests, p = 0.294, p = 0.941 and p = 0.402; F″–H″ : Untreated group, n = 6, paired t-tests, p = 0.502, p = 0.690, p = 0.610). Scale bars: A–C = 100 µm (inset = 20 µm); D = 1 mm; E = 50 µm.

    Article Snippet: 6F3D-biotin complexes were revealed with streptavadin-horseradish peroxidase (Vectastain Elite, Vector Laboratories, Burlingame, CA, USA) and DAB.

    Techniques: Mouse Assay, Staining, Software, Magnetic Resonance Imaging