strepavidin magnetic beads  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Streptavidin Magnetic Beads
    Description:
    Streptavidin Magnetic Beads 5 ml
    Catalog Number:
    s1420s
    Price:
    318
    Size:
    5 ml
    Category:
    Magnetic Separation Equipment
    Buy from Supplier


    Structured Review

    New England Biolabs strepavidin magnetic beads
    Streptavidin Magnetic Beads
    Streptavidin Magnetic Beads 5 ml
    https://www.bioz.com/result/strepavidin magnetic beads/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    strepavidin magnetic beads - by Bioz Stars, 2020-04
    99/100 stars

    Images

    Related Articles

    Luciferase:

    Article Title: La-related protein 1 (LARP1) repression of TOP mRNA translation is mediated through its cap-binding domain and controlled by an adjacent regulatory region
    Article Snippet: .. Materials Reagents were obtained from the following sources: antibodies for S6K, phospho-T389-S6K, eIF2α, phospho-Ser51-eIF2α, Raptor, mTOR, 4EBP1, LARP1, NCBP1, eIF4E, eIF4G and PABP from Cell Signaling Technology; primary antibodies for eIF3b and HRP-labeled secondary antibodies from Santa Cruz Biotechnology; IRDye secondary antibodies from LI-COR; Dulbecco’s modified Eagle’s medium (DMEM) from Life Technologies; heat-inactivated Fetal Bovine Serum (IFS) and 7mGDP from Sigma Aldrich; DNase I, T4 DNA ligase 1, T4 RNA Ligase I, T7 RNA polymerase, polynucleotide kinase, Protoscript II reverse transcriptase, Vaccinia Capping System, Oligo d(T)25 Magnetic beads and streptavidin-coated magnetic beads from New England Biolabs; iTaq Universal SYBR Green Supermix and Bradford Protein Assay from Bio-rad; RNeasy Plus Mini Kit from Qiagen; Dual Luciferase Assay from Promega; and X-tremeGENE 9 transfection reagent from Roche. .. Cell culture and preparation of cell extracts Cells were grown in high-glucose DMEM supplemented with 10% (v/v) heat-inactivated fetal bovine serum and penicillin-streptomycin.

    Bradford Protein Assay:

    Article Title: La-related protein 1 (LARP1) repression of TOP mRNA translation is mediated through its cap-binding domain and controlled by an adjacent regulatory region
    Article Snippet: .. Materials Reagents were obtained from the following sources: antibodies for S6K, phospho-T389-S6K, eIF2α, phospho-Ser51-eIF2α, Raptor, mTOR, 4EBP1, LARP1, NCBP1, eIF4E, eIF4G and PABP from Cell Signaling Technology; primary antibodies for eIF3b and HRP-labeled secondary antibodies from Santa Cruz Biotechnology; IRDye secondary antibodies from LI-COR; Dulbecco’s modified Eagle’s medium (DMEM) from Life Technologies; heat-inactivated Fetal Bovine Serum (IFS) and 7mGDP from Sigma Aldrich; DNase I, T4 DNA ligase 1, T4 RNA Ligase I, T7 RNA polymerase, polynucleotide kinase, Protoscript II reverse transcriptase, Vaccinia Capping System, Oligo d(T)25 Magnetic beads and streptavidin-coated magnetic beads from New England Biolabs; iTaq Universal SYBR Green Supermix and Bradford Protein Assay from Bio-rad; RNeasy Plus Mini Kit from Qiagen; Dual Luciferase Assay from Promega; and X-tremeGENE 9 transfection reagent from Roche. .. Cell culture and preparation of cell extracts Cells were grown in high-glucose DMEM supplemented with 10% (v/v) heat-inactivated fetal bovine serum and penicillin-streptomycin.

    Synthesized:

    Article Title: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf
    Article Snippet: .. Streptavidin-coated magnetic beads (1.1 mg/ml) were applied again to purify synthesized double-stranded cDNAs with three times washes. .. The double-stranded cDNAs were released from the magnetic beads by Not I (75 U) restriction digestion at 37°C for 1 h followed by the same volume phenol/chloroform/isoamyl alcohol (25:24:1) extraction and precipitation using 7.5 M sodium acetate, glycogen (20 μg/μl), and ice-cold absolute ethanol at -20°C overnight.

    Article Title: Simultaneous and stoichiometric purification of hundreds of oligonucleotides
    Article Snippet: The probe is synthesized as a randomer with degenerate nucleotides “SWSWSW” using a split-pool approach ; through the course of a single synthesis, all instances of the of the random sequences are created in roughly equal concentrations. .. Subsequent solid-phase separation using streptavidin-coated magnetic beads removes unbound precursors, and applying USER enzyme mix (New England Biolabs) cleaves the oligo products from the tags at the dU site.

    Construct:

    Article Title: Src kinase phosphorylates Notch1 to inhibit MAML binding
    Article Snippet: 293 T cells were Trans IT®-LT1 (Mirus) lipid transfected with either 2058–2161::BirA or N1ICD::BirA fusion constructs. .. Streptavidin magnetic beads (10 µL, New England BioLabs) were used to precipitate biotinylated species on a magnetic tube rack.

    SYBR Green Assay:

    Article Title: La-related protein 1 (LARP1) repression of TOP mRNA translation is mediated through its cap-binding domain and controlled by an adjacent regulatory region
    Article Snippet: .. Materials Reagents were obtained from the following sources: antibodies for S6K, phospho-T389-S6K, eIF2α, phospho-Ser51-eIF2α, Raptor, mTOR, 4EBP1, LARP1, NCBP1, eIF4E, eIF4G and PABP from Cell Signaling Technology; primary antibodies for eIF3b and HRP-labeled secondary antibodies from Santa Cruz Biotechnology; IRDye secondary antibodies from LI-COR; Dulbecco’s modified Eagle’s medium (DMEM) from Life Technologies; heat-inactivated Fetal Bovine Serum (IFS) and 7mGDP from Sigma Aldrich; DNase I, T4 DNA ligase 1, T4 RNA Ligase I, T7 RNA polymerase, polynucleotide kinase, Protoscript II reverse transcriptase, Vaccinia Capping System, Oligo d(T)25 Magnetic beads and streptavidin-coated magnetic beads from New England Biolabs; iTaq Universal SYBR Green Supermix and Bradford Protein Assay from Bio-rad; RNeasy Plus Mini Kit from Qiagen; Dual Luciferase Assay from Promega; and X-tremeGENE 9 transfection reagent from Roche. .. Cell culture and preparation of cell extracts Cells were grown in high-glucose DMEM supplemented with 10% (v/v) heat-inactivated fetal bovine serum and penicillin-streptomycin.

    Incubation:

    Article Title: Kinetic mechanism for viral dsRNA length discrimination by MDA5 filaments
    Article Snippet: .. The binding reaction was quenched with 60 μg/mL heparin and 2 mM ADP⋅AlF4 and was incubated with streptavidin magnetic beads (New England BioLabs). ..

    Article Title: miR-106b-responsive gene landscape identifies regulation of Kruppel-like factor family
    Article Snippet: .. 90% of cell lysate was incubated with streptavidin magnetic beads (New England Biolabs) for 6 hours at 4°C and 10% of cell lysate was used for input RNA. ..

    Stripping Membranes:

    Article Title: Src kinase phosphorylates Notch1 to inhibit MAML binding
    Article Snippet: Streptavidin magnetic beads (10 µL, New England BioLabs) were used to precipitate biotinylated species on a magnetic tube rack. .. Specific protein targets were detected using primary antibodies followed by membrane stripping before detection of overall biotinylated proteins.

    Expressing:

    Article Title: miR-106b-responsive gene landscape identifies regulation of Kruppel-like factor family
    Article Snippet: 90% of cell lysate was incubated with streptavidin magnetic beads (New England Biolabs) for 6 hours at 4°C and 10% of cell lysate was used for input RNA. .. Relative expression of IL8, KLF2 and α Tubulin1A was analyzed by qRT-PCR.

    Modification:

    Article Title: La-related protein 1 (LARP1) repression of TOP mRNA translation is mediated through its cap-binding domain and controlled by an adjacent regulatory region
    Article Snippet: .. Materials Reagents were obtained from the following sources: antibodies for S6K, phospho-T389-S6K, eIF2α, phospho-Ser51-eIF2α, Raptor, mTOR, 4EBP1, LARP1, NCBP1, eIF4E, eIF4G and PABP from Cell Signaling Technology; primary antibodies for eIF3b and HRP-labeled secondary antibodies from Santa Cruz Biotechnology; IRDye secondary antibodies from LI-COR; Dulbecco’s modified Eagle’s medium (DMEM) from Life Technologies; heat-inactivated Fetal Bovine Serum (IFS) and 7mGDP from Sigma Aldrich; DNase I, T4 DNA ligase 1, T4 RNA Ligase I, T7 RNA polymerase, polynucleotide kinase, Protoscript II reverse transcriptase, Vaccinia Capping System, Oligo d(T)25 Magnetic beads and streptavidin-coated magnetic beads from New England Biolabs; iTaq Universal SYBR Green Supermix and Bradford Protein Assay from Bio-rad; RNeasy Plus Mini Kit from Qiagen; Dual Luciferase Assay from Promega; and X-tremeGENE 9 transfection reagent from Roche. .. Cell culture and preparation of cell extracts Cells were grown in high-glucose DMEM supplemented with 10% (v/v) heat-inactivated fetal bovine serum and penicillin-streptomycin.

    Hybridization:

    Article Title: Simultaneous and stoichiometric purification of hundreds of oligonucleotides
    Article Snippet: Subsequent solid-phase separation using streptavidin-coated magnetic beads removes unbound precursors, and applying USER enzyme mix (New England Biolabs) cleaves the oligo products from the tags at the dU site. .. The entire SNOP protocol takes roughly 3 h, with 30 min being hands-on time and the remainder being waiting time for hybridization and digestion.

    High Performance Liquid Chromatography:

    Article Title: Simultaneous and stoichiometric purification of hundreds of oligonucleotides
    Article Snippet: Subsequent solid-phase separation using streptavidin-coated magnetic beads removes unbound precursors, and applying USER enzyme mix (New England Biolabs) cleaves the oligo products from the tags at the dU site. .. This time is constant regardless of the number of different precursor and oligo product species, rendering SNOP significantly more scalable than traditional HPLC or PAGE post-synthesis purification methods.

    Transfection:

    Article Title: La-related protein 1 (LARP1) repression of TOP mRNA translation is mediated through its cap-binding domain and controlled by an adjacent regulatory region
    Article Snippet: .. Materials Reagents were obtained from the following sources: antibodies for S6K, phospho-T389-S6K, eIF2α, phospho-Ser51-eIF2α, Raptor, mTOR, 4EBP1, LARP1, NCBP1, eIF4E, eIF4G and PABP from Cell Signaling Technology; primary antibodies for eIF3b and HRP-labeled secondary antibodies from Santa Cruz Biotechnology; IRDye secondary antibodies from LI-COR; Dulbecco’s modified Eagle’s medium (DMEM) from Life Technologies; heat-inactivated Fetal Bovine Serum (IFS) and 7mGDP from Sigma Aldrich; DNase I, T4 DNA ligase 1, T4 RNA Ligase I, T7 RNA polymerase, polynucleotide kinase, Protoscript II reverse transcriptase, Vaccinia Capping System, Oligo d(T)25 Magnetic beads and streptavidin-coated magnetic beads from New England Biolabs; iTaq Universal SYBR Green Supermix and Bradford Protein Assay from Bio-rad; RNeasy Plus Mini Kit from Qiagen; Dual Luciferase Assay from Promega; and X-tremeGENE 9 transfection reagent from Roche. .. Cell culture and preparation of cell extracts Cells were grown in high-glucose DMEM supplemented with 10% (v/v) heat-inactivated fetal bovine serum and penicillin-streptomycin.

    Article Title: miR-106b-responsive gene landscape identifies regulation of Kruppel-like factor family
    Article Snippet: Pull down of biotinylated RNA : Mz-ChA-1 cells were transfected with 50 nM of biotinylated microRNA using Lipofectamine RNAiMAX in triplicate. .. 90% of cell lysate was incubated with streptavidin magnetic beads (New England Biolabs) for 6 hours at 4°C and 10% of cell lysate was used for input RNA.

    Ligation:

    Article Title: Src kinase phosphorylates Notch1 to inhibit MAML binding
    Article Snippet: Paragraph title: Proximity biotin ligation assays ... Streptavidin magnetic beads (10 µL, New England BioLabs) were used to precipitate biotinylated species on a magnetic tube rack.

    Protease Inhibitor:

    Article Title: miR-106b-responsive gene landscape identifies regulation of Kruppel-like factor family
    Article Snippet: After 24 hours, cells were lysed (20 mM Tris, pH 7.5, 100 mM KCl, 5 mM MgCl2, 0.3% NP40, 50 U of RNase OUT and complete protease inhibitor) and incubated on ice for 10 minutes. .. 90% of cell lysate was incubated with streptavidin magnetic beads (New England Biolabs) for 6 hours at 4°C and 10% of cell lysate was used for input RNA.

    Binding Assay:

    Article Title: Kinetic mechanism for viral dsRNA length discrimination by MDA5 filaments
    Article Snippet: .. The binding reaction was quenched with 60 μg/mL heparin and 2 mM ADP⋅AlF4 and was incubated with streptavidin magnetic beads (New England BioLabs). ..

    Pull Down Assay:

    Article Title: Lactose repressor hinge domain independently binds DNA
    Article Snippet: Paragraph title: Magnetic bead DNA pull‐down assay ... Streptavidin magnetic beads (New England BioLabs) were placed in a magnetic tube rack and the supernatant removed.

    Magnetic Beads:

    Article Title: Lactose repressor hinge domain independently binds DNA
    Article Snippet: .. Streptavidin magnetic beads (New England BioLabs) were placed in a magnetic tube rack and the supernatant removed. ..

    Article Title: The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell–cell adhesion
    Article Snippet: .. Recombinant protein pull downs 25–50 μg (tandem or single domain, respectively) of biotinylated His.TEV.Avi.PTPx domains were conjugated to 167 μl of pre-washed streptavidin-coated magnetic beads suspension (4 mg/ml; New England Biolabs) in 500 μl of ice-cold size exclusion buffer (50 mM HEPES pH 7.5 (50 mM Tris pH 7.4 for SH2 domains), 150 mM NaCl, 5% (v/v) glycerol, 5 mM DTT) at 4°C for 1–2 hr on a rotator. ..

    Article Title: Src kinase phosphorylates Notch1 to inhibit MAML binding
    Article Snippet: .. Streptavidin magnetic beads (10 µL, New England BioLabs) were used to precipitate biotinylated species on a magnetic tube rack. .. Specific protein targets were detected using primary antibodies followed by membrane stripping before detection of overall biotinylated proteins.

    Article Title: Kinetic mechanism for viral dsRNA length discrimination by MDA5 filaments
    Article Snippet: .. The binding reaction was quenched with 60 μg/mL heparin and 2 mM ADP⋅AlF4 and was incubated with streptavidin magnetic beads (New England BioLabs). ..

    Article Title: La-related protein 1 (LARP1) repression of TOP mRNA translation is mediated through its cap-binding domain and controlled by an adjacent regulatory region
    Article Snippet: .. Materials Reagents were obtained from the following sources: antibodies for S6K, phospho-T389-S6K, eIF2α, phospho-Ser51-eIF2α, Raptor, mTOR, 4EBP1, LARP1, NCBP1, eIF4E, eIF4G and PABP from Cell Signaling Technology; primary antibodies for eIF3b and HRP-labeled secondary antibodies from Santa Cruz Biotechnology; IRDye secondary antibodies from LI-COR; Dulbecco’s modified Eagle’s medium (DMEM) from Life Technologies; heat-inactivated Fetal Bovine Serum (IFS) and 7mGDP from Sigma Aldrich; DNase I, T4 DNA ligase 1, T4 RNA Ligase I, T7 RNA polymerase, polynucleotide kinase, Protoscript II reverse transcriptase, Vaccinia Capping System, Oligo d(T)25 Magnetic beads and streptavidin-coated magnetic beads from New England Biolabs; iTaq Universal SYBR Green Supermix and Bradford Protein Assay from Bio-rad; RNeasy Plus Mini Kit from Qiagen; Dual Luciferase Assay from Promega; and X-tremeGENE 9 transfection reagent from Roche. .. Cell culture and preparation of cell extracts Cells were grown in high-glucose DMEM supplemented with 10% (v/v) heat-inactivated fetal bovine serum and penicillin-streptomycin.

    Article Title: The Conserved C-Terminus of the PcrA/UvrD Helicase Interacts Directly with RNA Polymerase
    Article Snippet: .. Pull down experiments Pull down experiments were carried out using streptavidin magnetic beads (New England Biolabs). .. Each addition or washing step was performed at 4 °C by placing Eppendorf tubes containing beads on a rotator device, designed to continually but gently mix the beads with the sample.

    Article Title: miR-106b-responsive gene landscape identifies regulation of Kruppel-like factor family
    Article Snippet: .. 90% of cell lysate was incubated with streptavidin magnetic beads (New England Biolabs) for 6 hours at 4°C and 10% of cell lysate was used for input RNA. ..

    Article Title: Novel high-resolution characterization of ancient DNA reveals C > U-type base modification events as the sole cause of post mortem miscoding lesions
    Article Snippet: .. Bead washing Aliquots of 20 μl of Streptavidin magnetic beads (New England Biolabs, S1420S) were pre-washed three times with 2× BW buffer ( ); resuspended in 50 μl 2× BW; mixed with the 50 μl SPEX primer extension reaction and rotated at room temperature for 30 min to immobilize biotinylated molecules to the beads; then a series of washes with 2× BW, 0.15 M NaOH and 1× Tris/EDTA (TE, pH 7.5) were carried out as described by Chen et al. ( ) to remove everything but biotinylated molecules. .. The beads were resuspended to 14 μl with 0.1× Qiagen buffer EB (10 mM Tris·Cl, pH 8.5).

    Article Title: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf
    Article Snippet: .. Streptavidin-coated magnetic beads (1.1 mg/ml) were applied again to purify synthesized double-stranded cDNAs with three times washes. .. The double-stranded cDNAs were released from the magnetic beads by Not I (75 U) restriction digestion at 37°C for 1 h followed by the same volume phenol/chloroform/isoamyl alcohol (25:24:1) extraction and precipitation using 7.5 M sodium acetate, glycogen (20 μg/μl), and ice-cold absolute ethanol at -20°C overnight.

    Article Title: Aberrant Glycosylation in the Human Trabecular Meshwork
    Article Snippet: .. The precipitate was recovered with 25 μl of 4μg/μl streptavidin coupled magnetic beads (cat# S1420S, New England Biolabs, Ipswitch, MA). ..

    Article Title: Simultaneous and stoichiometric purification of hundreds of oligonucleotides
    Article Snippet: .. Subsequent solid-phase separation using streptavidin-coated magnetic beads removes unbound precursors, and applying USER enzyme mix (New England Biolabs) cleaves the oligo products from the tags at the dU site. .. SNOP, in essence, selects for precursors with perfectly synthesized tag sequences in a multiplexed fashion.

    Isolation:

    Article Title: miR-106b-responsive gene landscape identifies regulation of Kruppel-like factor family
    Article Snippet: 90% of cell lysate was incubated with streptavidin magnetic beads (New England Biolabs) for 6 hours at 4°C and 10% of cell lysate was used for input RNA. .. After pull down, RNA was isolated using mir Vana kit.

    Labeling:

    Article Title: Kinetic mechanism for viral dsRNA length discrimination by MDA5 filaments
    Article Snippet: For binding kinetics, labeled MDA5 (0.3 μM) was incubated with 3′-biotinylated dsRNA (0.6 μg/mL) at 37 °C in buffer A with 2 mM ADPCP for the indicated time periods. .. The binding reaction was quenched with 60 μg/mL heparin and 2 mM ADP⋅AlF4 and was incubated with streptavidin magnetic beads (New England BioLabs).

    Article Title: Aberrant Glycosylation in the Human Trabecular Meshwork
    Article Snippet: We utilized 12 glaucomatous and 12 normal samples for 8 plex ITRAQ labeling using a kit (cat# 4390811, Applied Biosystems, Carlsbad, CA) containing 113–119 and 121 isotopic tags following procedures routinely used in our laboratory [ ]. .. The precipitate was recovered with 25 μl of 4μg/μl streptavidin coupled magnetic beads (cat# S1420S, New England Biolabs, Ipswitch, MA).

    Purification:

    Article Title: Simultaneous and stoichiometric purification of hundreds of oligonucleotides
    Article Snippet: Subsequent solid-phase separation using streptavidin-coated magnetic beads removes unbound precursors, and applying USER enzyme mix (New England Biolabs) cleaves the oligo products from the tags at the dU site. .. This time is constant regardless of the number of different precursor and oligo product species, rendering SNOP significantly more scalable than traditional HPLC or PAGE post-synthesis purification methods.

    Dot Blot:

    Article Title: miR-106b-responsive gene landscape identifies regulation of Kruppel-like factor family
    Article Snippet: Biotinylated RNA was quantified by dot blot on hybond N+. .. 90% of cell lysate was incubated with streptavidin magnetic beads (New England Biolabs) for 6 hours at 4°C and 10% of cell lysate was used for input RNA.

    Quantitative RT-PCR:

    Article Title: miR-106b-responsive gene landscape identifies regulation of Kruppel-like factor family
    Article Snippet: 90% of cell lysate was incubated with streptavidin magnetic beads (New England Biolabs) for 6 hours at 4°C and 10% of cell lysate was used for input RNA. .. Relative expression of IL8, KLF2 and α Tubulin1A was analyzed by qRT-PCR.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Simultaneous and stoichiometric purification of hundreds of oligonucleotides
    Article Snippet: Subsequent solid-phase separation using streptavidin-coated magnetic beads removes unbound precursors, and applying USER enzyme mix (New England Biolabs) cleaves the oligo products from the tags at the dU site. .. This time is constant regardless of the number of different precursor and oligo product species, rendering SNOP significantly more scalable than traditional HPLC or PAGE post-synthesis purification methods.

    Concentration Assay:

    Article Title: Aberrant Glycosylation in the Human Trabecular Meshwork
    Article Snippet: The homogenized suspension was centrifuged at 10,000 rpm and precipitated using 10μl of a 9μg/μl working concentration of a biotinylated lectin, (for example, Caragana arborescens lectins; ). .. The precipitate was recovered with 25 μl of 4μg/μl streptavidin coupled magnetic beads (cat# S1420S, New England Biolabs, Ipswitch, MA).

    Recombinant:

    Article Title: The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell–cell adhesion
    Article Snippet: .. Recombinant protein pull downs 25–50 μg (tandem or single domain, respectively) of biotinylated His.TEV.Avi.PTPx domains were conjugated to 167 μl of pre-washed streptavidin-coated magnetic beads suspension (4 mg/ml; New England Biolabs) in 500 μl of ice-cold size exclusion buffer (50 mM HEPES pH 7.5 (50 mM Tris pH 7.4 for SH2 domains), 150 mM NaCl, 5% (v/v) glycerol, 5 mM DTT) at 4°C for 1–2 hr on a rotator. ..

    Immunoprecipitation:

    Article Title: Aberrant Glycosylation in the Human Trabecular Meshwork
    Article Snippet: Quantification of lectin immunoprecipitation products was done as follows: briefly, protein extracts were prepared from glaucomatous and normal donor TM using 100μl of the buffer 125 mM Tris-HCl (pH 7.0), 100 mM NaCl, 0.1% Triton X-100, and 0.1% Genapol C-100 per 25mg of tissue. .. The precipitate was recovered with 25 μl of 4μg/μl streptavidin coupled magnetic beads (cat# S1420S, New England Biolabs, Ipswitch, MA).

    Kinetic Assay:

    Article Title: Kinetic mechanism for viral dsRNA length discrimination by MDA5 filaments
    Article Snippet: Paragraph title: Pull-Down Kinetic Assay. ... The binding reaction was quenched with 60 μg/mL heparin and 2 mM ADP⋅AlF4 and was incubated with streptavidin magnetic beads (New England BioLabs).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs streptavidin coated magnetic beads
    General scheme applied for identifying peanut immature pod-specific genes (tracer mRNA (1)) after a single round subtraction . B biotin, S <t>streptavidin,</t> M magnetic bead.
    Streptavidin Coated Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin coated magnetic beads/product/New England Biolabs
    Average 99 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    streptavidin coated magnetic beads - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs magnetic hydrophilic streptavidin beads
    Overview of 3-day generation of cDNA libraries. ( A ) On the first day, total RNA is ligated to a 3′ adapter and cDNA is generated by reverse transcription by tandem reactions in a single tube, RNA is degraded and cDNAs are isolated by ethanol precipitation. ( B ) On the second day, cDNAs are circularized, size selected by gel fractionation and eluted overnight in the presence of <t>streptavidin</t> beads. ( C ) PCR is done on bead-bound purified cDNAs to generate templates ready for high-throughput sequencing.
    Magnetic Hydrophilic Streptavidin Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnetic hydrophilic streptavidin beads/product/New England Biolabs
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    magnetic hydrophilic streptavidin beads - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    General scheme applied for identifying peanut immature pod-specific genes (tracer mRNA (1)) after a single round subtraction . B biotin, S streptavidin, M magnetic bead.

    Journal: Biological Procedures Online

    Article Title: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf

    doi: 10.1007/s12575-009-9022-z

    Figure Lengend Snippet: General scheme applied for identifying peanut immature pod-specific genes (tracer mRNA (1)) after a single round subtraction . B biotin, S streptavidin, M magnetic bead.

    Article Snippet: Streptavidin-coated magnetic beads (1.1 mg/ml) were applied again to purify synthesized double-stranded cDNAs with three times washes.

    Techniques:

    Phosphorylation of tyrosine residues on the N1ICD are SFK dependent. ( A ) Diagram of BioID experiment. In the presence of biotin, the NICD::BirA fusion protein biotinylates nearby proteins which can be affinity captured through streptavidin purification. ( B ) Western blot analysis of affinity captured material from BioID experiment using a N1ICD::BirA-HA fusion protein in 293 T cells. Expression of Src variants is denoted as WT = wild-type, CA = constitutively active, and DN = dominant negative. ( C ) Amino acid sequence of 2058–2161::BirA-HA fusion protein containing a 104 amino acid fragment of N1ICD fused to a biotin ligase. ( D ) Western blot analysis of affinity captured material from BioID experiment using the 2058–2161::BirA-HA fusion in 293 T cells. ( E ) Western blot analysis of immunoprecipitated N1ICD in the presence or absence of c-Src overexpression in 293 T cells. ( F ) Western blot analysis of immunoprecipitated N1ICD under conditions SFK inhibition (AZM475271) and control in 293 T cells. ( G ) Western blot analysis of immunoprecipitated N1ICD and N4ICD in the presence or absence of c-Src overexpression in 293 T cells. In all panels, western blots depict representative images from experiments that were replicated at least three independent times.

    Journal: Scientific Reports

    Article Title: Src kinase phosphorylates Notch1 to inhibit MAML binding

    doi: 10.1038/s41598-018-33920-y

    Figure Lengend Snippet: Phosphorylation of tyrosine residues on the N1ICD are SFK dependent. ( A ) Diagram of BioID experiment. In the presence of biotin, the NICD::BirA fusion protein biotinylates nearby proteins which can be affinity captured through streptavidin purification. ( B ) Western blot analysis of affinity captured material from BioID experiment using a N1ICD::BirA-HA fusion protein in 293 T cells. Expression of Src variants is denoted as WT = wild-type, CA = constitutively active, and DN = dominant negative. ( C ) Amino acid sequence of 2058–2161::BirA-HA fusion protein containing a 104 amino acid fragment of N1ICD fused to a biotin ligase. ( D ) Western blot analysis of affinity captured material from BioID experiment using the 2058–2161::BirA-HA fusion in 293 T cells. ( E ) Western blot analysis of immunoprecipitated N1ICD in the presence or absence of c-Src overexpression in 293 T cells. ( F ) Western blot analysis of immunoprecipitated N1ICD under conditions SFK inhibition (AZM475271) and control in 293 T cells. ( G ) Western blot analysis of immunoprecipitated N1ICD and N4ICD in the presence or absence of c-Src overexpression in 293 T cells. In all panels, western blots depict representative images from experiments that were replicated at least three independent times.

    Article Snippet: Streptavidin magnetic beads (10 µL, New England BioLabs) were used to precipitate biotinylated species on a magnetic tube rack.

    Techniques: Purification, Western Blot, Expressing, Dominant Negative Mutation, Sequencing, Immunoprecipitation, Over Expression, Inhibition

    RNA-Seq target validation. (A) qRT-PCR for nine candidate targets from RNA-Seq. Relative expression is significantly increased for LNA-106b compared to miR-106b in seven of the genes. There was a trend towards increased expression for LNA-106b compared to miR-106b for NCEH1 but it was not statistically significant (p = 0.07). FOS showed no significant change in expression by qRT-PCR. Dotted line represents expression level for scrambled control LNA. ITGA3 and HRAS are non-target negative control genes which show no significant expression change. Relative expression is given on the left y-axis and fold change is shown on the right y-axis. (B) Immunoblot showing transfection with miR-106b decreased protein levels of DR5, RB1, and E2F1 in Mz-ChA-1 cells. (C) Schematic of experimental design for capture of mRNA targets using biotinylated microRNA. Briefly, Mz-ChA-1 cells were transfected for 24 hours with either mature human miR-106b or C. elegans miR-67 which had been biotinylated. Cells were lysed and incubated with streptavidin-bound beads to capture biotinylated microRNA and associated mRNAs. Total RNA was isolated and relative expression of target mRNAs KLF2 and IL-8 was measured for enrichment by qRT-PCR. 18S was used as a control RNA. * p

    Journal: RNA Biology

    Article Title: miR-106b-responsive gene landscape identifies regulation of Kruppel-like factor family

    doi: 10.1080/15476286.2017.1422471

    Figure Lengend Snippet: RNA-Seq target validation. (A) qRT-PCR for nine candidate targets from RNA-Seq. Relative expression is significantly increased for LNA-106b compared to miR-106b in seven of the genes. There was a trend towards increased expression for LNA-106b compared to miR-106b for NCEH1 but it was not statistically significant (p = 0.07). FOS showed no significant change in expression by qRT-PCR. Dotted line represents expression level for scrambled control LNA. ITGA3 and HRAS are non-target negative control genes which show no significant expression change. Relative expression is given on the left y-axis and fold change is shown on the right y-axis. (B) Immunoblot showing transfection with miR-106b decreased protein levels of DR5, RB1, and E2F1 in Mz-ChA-1 cells. (C) Schematic of experimental design for capture of mRNA targets using biotinylated microRNA. Briefly, Mz-ChA-1 cells were transfected for 24 hours with either mature human miR-106b or C. elegans miR-67 which had been biotinylated. Cells were lysed and incubated with streptavidin-bound beads to capture biotinylated microRNA and associated mRNAs. Total RNA was isolated and relative expression of target mRNAs KLF2 and IL-8 was measured for enrichment by qRT-PCR. 18S was used as a control RNA. * p

    Article Snippet: 90% of cell lysate was incubated with streptavidin magnetic beads (New England Biolabs) for 6 hours at 4°C and 10% of cell lysate was used for input RNA.

    Techniques: RNA Sequencing Assay, Quantitative RT-PCR, Expressing, Negative Control, Transfection, Incubation, Isolation

    Overview of 3-day generation of cDNA libraries. ( A ) On the first day, total RNA is ligated to a 3′ adapter and cDNA is generated by reverse transcription by tandem reactions in a single tube, RNA is degraded and cDNAs are isolated by ethanol precipitation. ( B ) On the second day, cDNAs are circularized, size selected by gel fractionation and eluted overnight in the presence of streptavidin beads. ( C ) PCR is done on bead-bound purified cDNAs to generate templates ready for high-throughput sequencing.

    Journal: Nucleic Acids Research

    Article Title: An efficient and sensitive method for preparing cDNA libraries from scarce biological samples

    doi: 10.1093/nar/gku637

    Figure Lengend Snippet: Overview of 3-day generation of cDNA libraries. ( A ) On the first day, total RNA is ligated to a 3′ adapter and cDNA is generated by reverse transcription by tandem reactions in a single tube, RNA is degraded and cDNAs are isolated by ethanol precipitation. ( B ) On the second day, cDNAs are circularized, size selected by gel fractionation and eluted overnight in the presence of streptavidin beads. ( C ) PCR is done on bead-bound purified cDNAs to generate templates ready for high-throughput sequencing.

    Article Snippet: 5.0 μl magnetic hydrophilic streptavidin beads (New England Biolabs) were washed three times with 50 μl buffer WB (0.5M NaCl, 20 mM Tris-HCl pH 7.5, 1.0 mM EDTA), resuspended in 5.0 μl TE + 0.3M NaCl and added to each sample.

    Techniques: Generated, Isolation, Ethanol Precipitation, Fractionation, Polymerase Chain Reaction, Purification, Next-Generation Sequencing

    Detailed LQ cloning method. ( A ) A pre-adenylated (rApp) 3′-terminal dideoxy-C (ddC) blocked adapter (gray) is annealed to a ssDNA reverse transcription (RT) oligo (black) in a 1:1 molar ratio. The annealed adapter is ligated to 3′-hydroxyl-containing RNA (orange) using T4 RNA Ligase 2 (truncated K227Q) without ATP. Each RT oligo contains a 5′ Guanine (G) followed by a 4 or 6 nucleotide randomer (N X ), a 3–6 nucleotide barcode (BAR) and 3 internal deoxyUridine (dU) nucleotides. The adapter::RT oligo hybrid is in excess over RNA, resulting in free adapter::primer material present in the completed reaction. ( B ) Reverse transcription of ligated RNA is carried out in the same tube as the ligation reaction generating ‘+ insert’ and ‘no insert’ cDNA products (red and black line) using dGTP, dTTP, dATP, dCTP as well as biotinylated dATP and dCTP (yellow ‘B’-containing circles). The RNA template is degraded (dashed orange line) by base hydrolysis and cDNA is ethanol precipitated with ammonium acetate to facilitate maximum removal of free adapter and unincorporated nucleotides ( C ). Ethanol precipitated cDNAs are circularized ( D ) and resolved on a 10% denaturing polyacrylamide gel. ‘+ insert’ circularized cDNAs are isolated by excising and eluting them from the gel overnight in the presence of magnetic streptavidin beads ( E ). Bead-bound ‘+ insert’ cDNAs serve as templates in the first round of PCR. Amplification is done using a mix containing uracil-N-deglycosylase (UNG) to remove dU nucleotides, thereby generating a linear template through strand scission, and with primers complimentary to the 3′ adapter (blue) and 5′ end of the RT oligo (tan) ( F ). First round PCR products are resolved on an 8% native polyacrylamide gel, the 60–70 nucleotide products are excised and a portion is used as the template for second round PCR. Second round PCR products are generated using primers complimentary to the 3′ adapter (dark blue) and 5′ end of the RT oligo (brown) that contain the full Illumina or Ion Torrent adapter sequences (dark blue and brown) ( G ).

    Journal: Nucleic Acids Research

    Article Title: An efficient and sensitive method for preparing cDNA libraries from scarce biological samples

    doi: 10.1093/nar/gku637

    Figure Lengend Snippet: Detailed LQ cloning method. ( A ) A pre-adenylated (rApp) 3′-terminal dideoxy-C (ddC) blocked adapter (gray) is annealed to a ssDNA reverse transcription (RT) oligo (black) in a 1:1 molar ratio. The annealed adapter is ligated to 3′-hydroxyl-containing RNA (orange) using T4 RNA Ligase 2 (truncated K227Q) without ATP. Each RT oligo contains a 5′ Guanine (G) followed by a 4 or 6 nucleotide randomer (N X ), a 3–6 nucleotide barcode (BAR) and 3 internal deoxyUridine (dU) nucleotides. The adapter::RT oligo hybrid is in excess over RNA, resulting in free adapter::primer material present in the completed reaction. ( B ) Reverse transcription of ligated RNA is carried out in the same tube as the ligation reaction generating ‘+ insert’ and ‘no insert’ cDNA products (red and black line) using dGTP, dTTP, dATP, dCTP as well as biotinylated dATP and dCTP (yellow ‘B’-containing circles). The RNA template is degraded (dashed orange line) by base hydrolysis and cDNA is ethanol precipitated with ammonium acetate to facilitate maximum removal of free adapter and unincorporated nucleotides ( C ). Ethanol precipitated cDNAs are circularized ( D ) and resolved on a 10% denaturing polyacrylamide gel. ‘+ insert’ circularized cDNAs are isolated by excising and eluting them from the gel overnight in the presence of magnetic streptavidin beads ( E ). Bead-bound ‘+ insert’ cDNAs serve as templates in the first round of PCR. Amplification is done using a mix containing uracil-N-deglycosylase (UNG) to remove dU nucleotides, thereby generating a linear template through strand scission, and with primers complimentary to the 3′ adapter (blue) and 5′ end of the RT oligo (tan) ( F ). First round PCR products are resolved on an 8% native polyacrylamide gel, the 60–70 nucleotide products are excised and a portion is used as the template for second round PCR. Second round PCR products are generated using primers complimentary to the 3′ adapter (dark blue) and 5′ end of the RT oligo (brown) that contain the full Illumina or Ion Torrent adapter sequences (dark blue and brown) ( G ).

    Article Snippet: 5.0 μl magnetic hydrophilic streptavidin beads (New England Biolabs) were washed three times with 50 μl buffer WB (0.5M NaCl, 20 mM Tris-HCl pH 7.5, 1.0 mM EDTA), resuspended in 5.0 μl TE + 0.3M NaCl and added to each sample.

    Techniques: Clone Assay, Ligation, Isolation, Polymerase Chain Reaction, Amplification, Generated