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GE Healthcare strep tactin hrp detection solution
Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a <t>Strep-Tactin®-HRP</t> conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).
Strep Tactin Hrp Detection Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/strep tactin hrp detection solution/product/GE Healthcare
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
strep tactin hrp detection solution - by Bioz Stars, 2020-09
90/100 stars

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1) Product Images from "Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems"

Article Title: Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems

Journal: PLoS ONE

doi: 10.1371/journal.pone.0152386

Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a Strep-Tactin®-HRP conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).
Figure Legend Snippet: Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a Strep-Tactin®-HRP conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).

Techniques Used: Expressing, Transfection, Sequencing, Fluorescence, Microscopy, Western Blot

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Article Title: Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems
Article Snippet: .. The membrane was blocked with 1 x PBS, 0.5% Tween 20 supplemented with 3% BSA for 1 h. Then the block solution was discarded and Strep-Tactin®-HRP detection solution (1:100000 dilution in 1 x PBS, 0.1% Tween 20) was directly added to the membrane for 1 h. The membrane was then washed twice with 1 x PBS, 0.1% Tween-20 and twice with 1 x PBS for 1 min before the membrane was developed using ECL™ (GE Healthcare Europe GmbH, Freiburg, Germany, Cat. no.: RPN2232). ..

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    GE Healthcare strep tactin hrp detection solution
    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a <t>Strep-Tactin®-HRP</t> conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).
    Strep Tactin Hrp Detection Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/strep tactin hrp detection solution/product/GE Healthcare
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    strep tactin hrp detection solution - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

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    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a Strep-Tactin®-HRP conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).

    Journal: PLoS ONE

    Article Title: Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems

    doi: 10.1371/journal.pone.0152386

    Figure Lengend Snippet: Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a Strep-Tactin®-HRP conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).

    Article Snippet: The membrane was blocked with 1 x PBS, 0.5% Tween 20 supplemented with 3% BSA for 1 h. Then the block solution was discarded and Strep-Tactin®-HRP detection solution (1:100000 dilution in 1 x PBS, 0.1% Tween 20) was directly added to the membrane for 1 h. The membrane was then washed twice with 1 x PBS, 0.1% Tween-20 and twice with 1 x PBS for 1 min before the membrane was developed using ECL™ (GE Healthcare Europe GmbH, Freiburg, Germany, Cat. no.: RPN2232).

    Techniques: Expressing, Transfection, Sequencing, Fluorescence, Microscopy, Western Blot