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Live <t>3D-holotomographic</t> microscopy analyses of bovine neutrophils exposed to Calicophoron daubneyi unveil degranulation and chemotaxis events. (A) Calicophoron daubneyi antigen (100 µg/mL, CdAg) induced degranulation of neutrophils (arrows) as early as 5 min incubation until the end of the experiment. (B) <t>3D</t> <t>reconstruction</t> and digital staining of holotomographic images confirm shift and accumulation of granules at one side of the neutrophil (zoom, blue arrow). (C) Chemotactic events are recorded between 25 and 54 min of incubation (yellow arrows) with displacement of stimulated neutrophils with CdAg 100 µg/mL. (D) Chemotaxis can be observed after co-culture of neutrophils with Calicophoron daubneyi eggs and analyzed via SEM. Neutrophils are elongated and moving in the direction of the eggs (yellow arrows), while other neutrophils are activated and cast short spr NETs (white arrows) in the vicinity of the eggs. (E) Elongated neutrophils in detail. Rupture is an artefact caused by the SEM analyses.
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software steve - by Bioz Stars, 2026-11
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  Buy from Supplier

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Live 3D-holotomographic microscopy analyses of bovine neutrophils exposed to Calicophoron daubneyi unveil degranulation and chemotaxis events. (A) Calicophoron daubneyi antigen (100 µg/mL, CdAg) induced degranulation of neutrophils (arrows) as early as 5 min incubation until the end of the experiment. (B) 3D reconstruction and digital staining of holotomographic images confirm shift and accumulation of granules at one side of the neutrophil (zoom, blue arrow). (C) Chemotactic events are recorded between 25 and 54 min of incubation (yellow arrows) with displacement of stimulated neutrophils with CdAg 100 µg/mL. (D) Chemotaxis can be observed after co-culture of neutrophils with Calicophoron daubneyi eggs and analyzed via SEM. Neutrophils are elongated and moving in the direction of the eggs (yellow arrows), while other neutrophils are activated and cast short spr NETs (white arrows) in the vicinity of the eggs. (E) Elongated neutrophils in detail. Rupture is an artefact caused by the SEM analyses.

Journal: Frontiers in Immunology

Article Title: Cellular immune responses of bovine polymorphonuclear neutrophils to Calicophoron daubneyi

doi: 10.3389/fimmu.2025.1515419

Figure Lengend Snippet: Live 3D-holotomographic microscopy analyses of bovine neutrophils exposed to Calicophoron daubneyi unveil degranulation and chemotaxis events. (A) Calicophoron daubneyi antigen (100 µg/mL, CdAg) induced degranulation of neutrophils (arrows) as early as 5 min incubation until the end of the experiment. (B) 3D reconstruction and digital staining of holotomographic images confirm shift and accumulation of granules at one side of the neutrophil (zoom, blue arrow). (C) Chemotactic events are recorded between 25 and 54 min of incubation (yellow arrows) with displacement of stimulated neutrophils with CdAg 100 µg/mL. (D) Chemotaxis can be observed after co-culture of neutrophils with Calicophoron daubneyi eggs and analyzed via SEM. Neutrophils are elongated and moving in the direction of the eggs (yellow arrows), while other neutrophils are activated and cast short spr NETs (white arrows) in the vicinity of the eggs. (E) Elongated neutrophils in detail. Rupture is an artefact caused by the SEM analyses.

Article Snippet: For the 3D reconstruction images, the software Steve (Nanolive) was used.

Techniques: Microscopy, Chemotaxis Assay, Incubation, Staining, Co-Culture Assay