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Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of <t>22L</t> prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of <t>PBS,</t> were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.
Sterile Pbs Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sterile pbs solution/product/Bio-Rad
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
sterile pbs solution - by Bioz Stars, 2020-09
92/100 stars

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1) Product Images from "Low doses of bioherbicide favour prion aggregation and propagation in vivo"

Article Title: Low doses of bioherbicide favour prion aggregation and propagation in vivo

Journal: Scientific Reports

doi: 10.1038/s41598-018-25966-9

Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of 22L prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of PBS, were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.
Figure Legend Snippet: Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of 22L prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of PBS, were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.

Techniques Used: Mouse Assay, Positron Emission Tomography

2) Product Images from "Low doses of bioherbicide favour prion aggregation and propagation in vivo"

Article Title: Low doses of bioherbicide favour prion aggregation and propagation in vivo

Journal: Scientific Reports

doi: 10.1038/s41598-018-25966-9

Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of 22L prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of PBS, were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.
Figure Legend Snippet: Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of 22L prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of PBS, were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.

Techniques Used: Mouse Assay, Positron Emission Tomography

Related Articles

Infection:

Article Title: Low doses of bioherbicide favour prion aggregation and propagation in vivo
Article Snippet: .. Aggregation assay Brains from a terminally sick mouse (infected with 22L prions) or from a non-infected mouse were homogenized in 10% (w/v) sterile PBS solution using microbead-containing tubes and a Ribolysor apparatus (Biorad, Marnes la Coquette, France). ..

Article Title: Low doses of bioherbicide favour prion aggregation and propagation in vivo
Article Snippet: .. Brains from a terminally sick mouse (infected with 22L prions) or from a non-infected mouse were homogenized in 10% (w/v) sterile PBS solution using microbead-containing tubes and a Ribolysor apparatus (Biorad, Marnes la Coquette, France). ..

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    Bio-Rad sterile pbs solution
    Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of <t>22L</t> prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of <t>PBS,</t> were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.
    Sterile Pbs Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sterile pbs solution/product/Bio-Rad
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    sterile pbs solution - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of 22L prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of PBS, were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.

    Journal: Scientific Reports

    Article Title: Low doses of bioherbicide favour prion aggregation and propagation in vivo

    doi: 10.1038/s41598-018-25966-9

    Figure Lengend Snippet: Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of 22L prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of PBS, were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.

    Article Snippet: Brains from a terminally sick mouse (infected with 22L prions) or from a non-infected mouse were homogenized in 10% (w/v) sterile PBS solution using microbead-containing tubes and a Ribolysor apparatus (Biorad, Marnes la Coquette, France).

    Techniques: Mouse Assay, Positron Emission Tomography

    Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of 22L prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of PBS, were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.

    Journal: Scientific Reports

    Article Title: Low doses of bioherbicide favour prion aggregation and propagation in vivo

    doi: 10.1038/s41598-018-25966-9

    Figure Lengend Snippet: Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of 22L prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of PBS, were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.

    Article Snippet: Aggregation assay Brains from a terminally sick mouse (infected with 22L prions) or from a non-infected mouse were homogenized in 10% (w/v) sterile PBS solution using microbead-containing tubes and a Ribolysor apparatus (Biorad, Marnes la Coquette, France).

    Techniques: Mouse Assay, Positron Emission Tomography