steponeplus real time pcr system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher steponeplus real time pcr system
    Steponeplus Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/steponeplus real time pcr system/product/Thermo Fisher
    Average 99 stars, based on 3203 article reviews
    Price from $9.99 to $1999.99
    steponeplus real time pcr system - by Bioz Stars, 2020-04
    99/100 stars

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    Amplification:

    Article Title: A developmental program truncates long transcripts to temporally regulate cell signaling
    Article Snippet: RNA was treated with DNase I (NEB) and reverse transcribed using Protoscript II (NEB). qPCR was performed on cDNA using SYBR Green I Master Mix (Roche) on a StepOnePlus Real-Time PCR System (Applied Biosciences) using primers listed in . .. For long genes sog, NetA, Pka-C3 , and sca , primers used all amplified the 5’ exons of the genes expressed as part of the short forms.

    Article Title: The role of the bacterial protease Prc in the uropathogenesis of extraintestinal pathogenic Escherichia coli
    Article Snippet: For qPCR, the cDNA and primers were mixed with KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems, Boston, MA, USA) and then subjected to PCR using a StepOnePlus™ Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). .. For RT-PCR, the cDNA was subjected to PCR amplification using Taq polymerase for 25 cycles.

    Article Title: Serological and Molecular Identification of Brucella spp. in Pigs from Cairo and Giza Governorates, Egypt
    Article Snippet: .. Amplification and real-time fluorescence detection was carried out on a StepOnePlus™ Real-Time PCR System (Applied Biosystems® , Germany). ..

    Synthesized:

    Article Title: Expression Analysis of the PITX2 Gene and Associations between Its Polymorphisms and Body Size and Carcass Traits in Chickens
    Article Snippet: Primers were synthesized by Tsingke Biotech Co., Ltd. (Hangzhou, China). .. Real-time PCR was performed using a StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with a 20 μL PCR mixture including 2 μL of cDNA, 10 μL of 2 × SYBR Premix Ex TaqII, 0.4 μL ROX Reference Dye (50×) 0.8 μL of forward primer, 0.8 μL of reverse primer and 6 μL of nuclease-free water.

    Article Title: RNA‐binding protein NONO promotes breast cancer proliferation by post‐transcriptional regulation of SKP2 and E2F8, et al. RNA‐binding protein NONO promotes breast cancer proliferation by post‐transcriptional regulation of SKP2 and E2F8
    Article Snippet: .. 2.8 Quantitative reverse transcription polymerase chain reaction RNA was extracted from cells using ISOGEN (Nippon Gene) and single‐stranded cDNA was synthesized using Super Script III reverse transcriptase (Invitrogen) with oligo dT or random primers. qRT‐PCR was carried out on StepOnePlus Real‐Time PCR System (Thermo Fisher Scientific) using KAPA SYBR FAST qPCR Kit (KAPA Biosystems) and sets of gene‐specific primers. ..

    Quantitative RT-PCR:

    Article Title: Knockout of beta‐2 microglobulin reduces stem cell‐induced immune rejection and enhances ischaemic hindlimb repair via exosome/miR‐24/Bim pathway, et al. Knockout of beta‐2 microglobulin reduces stem cell‐induced immune rejection and enhances ischaemic hindlimb repair via exosome/miR‐24/Bim pathway
    Article Snippet: .. The expression levels of miRNAs were analysed by qRT‐PCR using Takara SYBR Premix Ex Taq (Tli RNaseH Plus) in a StepOnePlus Real‐Time PCR system (Applied Biosystems). ..

    Article Title: Elevated Intraocular Pressure Causes Abnormal Reactivity of Mouse Retinal Arterioles
    Article Snippet: .. Quantitative real-time RT-PCR (qPCR) reactions were performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems) using SYBR® Green JumpStart™ Taq ReadyMix™ (Sigma-Aldrich, Munich, Germany) and 20 ng cDNA. ..

    Article Title: RNA‐binding protein NONO promotes breast cancer proliferation by post‐transcriptional regulation of SKP2 and E2F8, et al. RNA‐binding protein NONO promotes breast cancer proliferation by post‐transcriptional regulation of SKP2 and E2F8
    Article Snippet: .. 2.8 Quantitative reverse transcription polymerase chain reaction RNA was extracted from cells using ISOGEN (Nippon Gene) and single‐stranded cDNA was synthesized using Super Script III reverse transcriptase (Invitrogen) with oligo dT or random primers. qRT‐PCR was carried out on StepOnePlus Real‐Time PCR System (Thermo Fisher Scientific) using KAPA SYBR FAST qPCR Kit (KAPA Biosystems) and sets of gene‐specific primers. ..

    Article Title: The effects of transfer from steady-state to tidally-changing salinities on plasma and branchial osmoregulatory variables in adult Mozambique tilapia.
    Article Snippet: Paragraph title: 2.4. Quantitative real-time PCR (qRT-PCR) ... Quantitative real-time PCRs (qRT-PCRs) were set up as previously described , using the StepOnePlus real-time PCR system (Applied Biosystems).

    Article Title: RNF12 catalyzes BRF1 ubiquitination and regulates RNA polymerase III–dependent transcription
    Article Snippet: Paragraph title: Real-time RT–PCR ... Real-time PCR was performed using SYBR premix EX Taq (TaKaRa) and analyzed with the StepOnePlus real-time PCR system (Thermo Fisher Scientific).

    Real-time Polymerase Chain Reaction:

    Article Title: Expression Analysis of the PITX2 Gene and Associations between Its Polymorphisms and Body Size and Carcass Traits in Chickens
    Article Snippet: .. Real-time PCR was performed using a StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with a 20 μL PCR mixture including 2 μL of cDNA, 10 μL of 2 × SYBR Premix Ex TaqII, 0.4 μL ROX Reference Dye (50×) 0.8 μL of forward primer, 0.8 μL of reverse primer and 6 μL of nuclease-free water. .. The 2× SYBR Premix Ex TaqII, ROX Reference Dye (50×) and nuclease-free water were contained in the TB Green Premix Ex Taq TM II (Takara, Dalian, China).

    Article Title: Knockout of beta‐2 microglobulin reduces stem cell‐induced immune rejection and enhances ischaemic hindlimb repair via exosome/miR‐24/Bim pathway, et al. Knockout of beta‐2 microglobulin reduces stem cell‐induced immune rejection and enhances ischaemic hindlimb repair via exosome/miR‐24/Bim pathway
    Article Snippet: .. The expression levels of miRNAs were analysed by qRT‐PCR using Takara SYBR Premix Ex Taq (Tli RNaseH Plus) in a StepOnePlus Real‐Time PCR system (Applied Biosystems). ..

    Article Title: Elevated Intraocular Pressure Causes Abnormal Reactivity of Mouse Retinal Arterioles
    Article Snippet: .. Quantitative real-time RT-PCR (qPCR) reactions were performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems) using SYBR® Green JumpStart™ Taq ReadyMix™ (Sigma-Aldrich, Munich, Germany) and 20 ng cDNA. ..

    Article Title: Selective breeding modifies mef2ca mutant incomplete penetrance by tuning the opposing Notch pathway
    Article Snippet: .. The mef2ca b1086 mutant allele we exclusively use in this study and the KASP genotyping (LGC) protocol for this allele using a StepOnePlus Real-Time PCR System (Applied Biosystems) has been previously described [ , , ]. ..

    Article Title: A developmental program truncates long transcripts to temporally regulate cell signaling
    Article Snippet: .. RNA was treated with DNase I (NEB) and reverse transcribed using Protoscript II (NEB). qPCR was performed on cDNA using SYBR Green I Master Mix (Roche) on a StepOnePlus Real-Time PCR System (Applied Biosciences) using primers listed in . .. For long genes sog, NetA, Pka-C3 , and sca , primers used all amplified the 5’ exons of the genes expressed as part of the short forms.

    Article Title: The role of the bacterial protease Prc in the uropathogenesis of extraintestinal pathogenic Escherichia coli
    Article Snippet: .. For qPCR, the cDNA and primers were mixed with KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems, Boston, MA, USA) and then subjected to PCR using a StepOnePlus™ Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). .. The expression levels of the genes were normalized to those of ftsZ .

    Article Title: RNA‐binding protein NONO promotes breast cancer proliferation by post‐transcriptional regulation of SKP2 and E2F8, et al. RNA‐binding protein NONO promotes breast cancer proliferation by post‐transcriptional regulation of SKP2 and E2F8
    Article Snippet: .. 2.8 Quantitative reverse transcription polymerase chain reaction RNA was extracted from cells using ISOGEN (Nippon Gene) and single‐stranded cDNA was synthesized using Super Script III reverse transcriptase (Invitrogen) with oligo dT or random primers. qRT‐PCR was carried out on StepOnePlus Real‐Time PCR System (Thermo Fisher Scientific) using KAPA SYBR FAST qPCR Kit (KAPA Biosystems) and sets of gene‐specific primers. ..

    Article Title: Serological and Molecular Identification of Brucella spp. in Pigs from Cairo and Giza Governorates, Egypt
    Article Snippet: .. Amplification and real-time fluorescence detection was carried out on a StepOnePlus™ Real-Time PCR System (Applied Biosystems® , Germany). ..

    Article Title: The effects of transfer from steady-state to tidally-changing salinities on plasma and branchial osmoregulatory variables in adult Mozambique tilapia.
    Article Snippet: .. Quantitative real-time PCRs (qRT-PCRs) were set up as previously described , using the StepOnePlus real-time PCR system (Applied Biosystems). ..

    Article Title: Deep evolutionary origin of limb and fin regeneration
    Article Snippet: .. Gene-specific oligos for qPCR assays were designed using Primer Express Software v3.0 (Thermo Fisher Scientific) and used in a final concentration of 200 nM to each primer. qPCR was carried out using GoTaq Probe qPCR Master Mix (Promega) in a final volume of 10 μL, in a StepOnePlus Real-Time PCR System (Applied Biosystems), as previously described ( ). ..

    Article Title: RNF12 catalyzes BRF1 ubiquitination and regulates RNA polymerase III–dependent transcription
    Article Snippet: .. Real-time PCR was performed using SYBR premix EX Taq (TaKaRa) and analyzed with the StepOnePlus real-time PCR system (Thermo Fisher Scientific). .. The expression levels of the examined RNA were normalized to acidic ribosomal phosphoprotein P0.

    Random Hexamer Labeling:

    Article Title: The role of the bacterial protease Prc in the uropathogenesis of extraintestinal pathogenic Escherichia coli
    Article Snippet: Real-time quantitative PCR (qPCR) and reverse transcription-PCR (RT-PCR) The purified RNA was reverse transcribed into cDNA by using random hexamer primers and Moloney murine leukemia virus (M-MLV) reverse transcriptase according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA, USA). .. For qPCR, the cDNA and primers were mixed with KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems, Boston, MA, USA) and then subjected to PCR using a StepOnePlus™ Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA).

    Expressing:

    Article Title: Expression Analysis of the PITX2 Gene and Associations between Its Polymorphisms and Body Size and Carcass Traits in Chickens
    Article Snippet: Paragraph title: 2.4. Relative Expression of PITX2 mRNA ... Real-time PCR was performed using a StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with a 20 μL PCR mixture including 2 μL of cDNA, 10 μL of 2 × SYBR Premix Ex TaqII, 0.4 μL ROX Reference Dye (50×) 0.8 μL of forward primer, 0.8 μL of reverse primer and 6 μL of nuclease-free water.

    Article Title: Knockout of beta‐2 microglobulin reduces stem cell‐induced immune rejection and enhances ischaemic hindlimb repair via exosome/miR‐24/Bim pathway, et al. Knockout of beta‐2 microglobulin reduces stem cell‐induced immune rejection and enhances ischaemic hindlimb repair via exosome/miR‐24/Bim pathway
    Article Snippet: .. The expression levels of miRNAs were analysed by qRT‐PCR using Takara SYBR Premix Ex Taq (Tli RNaseH Plus) in a StepOnePlus Real‐Time PCR system (Applied Biosystems). ..

    Article Title: The role of the bacterial protease Prc in the uropathogenesis of extraintestinal pathogenic Escherichia coli
    Article Snippet: For qPCR, the cDNA and primers were mixed with KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems, Boston, MA, USA) and then subjected to PCR using a StepOnePlus™ Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). .. The expression levels of the genes were normalized to those of ftsZ .

    Article Title: The effects of transfer from steady-state to tidally-changing salinities on plasma and branchial osmoregulatory variables in adult Mozambique tilapia.
    Article Snippet: Quantitative real-time PCRs (qRT-PCRs) were set up as previously described , using the StepOnePlus real-time PCR system (Applied Biosystems). .. Elongation factor 1α (EF1α) was used as a reference gene to normalize the mRNA levels of target genes after it was verified that ef1α mRNA expression did not vary across treatments.

    Article Title: RNF12 catalyzes BRF1 ubiquitination and regulates RNA polymerase III–dependent transcription
    Article Snippet: Real-time PCR was performed using SYBR premix EX Taq (TaKaRa) and analyzed with the StepOnePlus real-time PCR system (Thermo Fisher Scientific). .. The expression levels of the examined RNA were normalized to acidic ribosomal phosphoprotein P0.

    Immunoprecipitation:

    Article Title: A developmental program truncates long transcripts to temporally regulate cell signaling
    Article Snippet: Immunoprecipitated material was eluted with 100ul of 50mM HEPES pH 7.4, 2% Sarkosyl, and 10mM DTT for 30 minutes at 50°C. .. RNA was treated with DNase I (NEB) and reverse transcribed using Protoscript II (NEB). qPCR was performed on cDNA using SYBR Green I Master Mix (Roche) on a StepOnePlus Real-Time PCR System (Applied Biosciences) using primers listed in .

    Generated:

    Article Title: Elevated Intraocular Pressure Causes Abnormal Reactivity of Mouse Retinal Arterioles
    Article Snippet: RNA was isolated using peqGOLD TriFast™ (PEQLAB), and cDNA was generated with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Darmstadt, Germany). .. Quantitative real-time RT-PCR (qPCR) reactions were performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems) using SYBR® Green JumpStart™ Taq ReadyMix™ (Sigma-Aldrich, Munich, Germany) and 20 ng cDNA.

    Article Title: The effects of transfer from steady-state to tidally-changing salinities on plasma and branchial osmoregulatory variables in adult Mozambique tilapia.
    Article Snippet: Quantitative real-time PCRs (qRT-PCRs) were set up as previously described , using the StepOnePlus real-time PCR system (Applied Biosystems). .. Standard curves for quantification were generated using serially diluted target gene cDNA fragments of known concentration (standard cDNAs).

    Polymerase Chain Reaction:

    Article Title: Expression Analysis of the PITX2 Gene and Associations between Its Polymorphisms and Body Size and Carcass Traits in Chickens
    Article Snippet: .. Real-time PCR was performed using a StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with a 20 μL PCR mixture including 2 μL of cDNA, 10 μL of 2 × SYBR Premix Ex TaqII, 0.4 μL ROX Reference Dye (50×) 0.8 μL of forward primer, 0.8 μL of reverse primer and 6 μL of nuclease-free water. .. The 2× SYBR Premix Ex TaqII, ROX Reference Dye (50×) and nuclease-free water were contained in the TB Green Premix Ex Taq TM II (Takara, Dalian, China).

    Article Title: The role of the bacterial protease Prc in the uropathogenesis of extraintestinal pathogenic Escherichia coli
    Article Snippet: .. For qPCR, the cDNA and primers were mixed with KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems, Boston, MA, USA) and then subjected to PCR using a StepOnePlus™ Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). .. The expression levels of the genes were normalized to those of ftsZ .

    Article Title: Serological and Molecular Identification of Brucella spp. in Pigs from Cairo and Giza Governorates, Egypt
    Article Snippet: Briefly, the PCR reaction was done in a 15 μL multiplex PCR mixture with 2× TaqMan™ Environmental master mix (Applied Biosystems® , Germany), 0.2 µM of each primer, 0.1 µM of each probe, and 5 μL of template DNA. .. Amplification and real-time fluorescence detection was carried out on a StepOnePlus™ Real-Time PCR System (Applied Biosystems® , Germany).

    Binding Assay:

    Article Title: A developmental program truncates long transcripts to temporally regulate cell signaling
    Article Snippet: Antibody-Protein G complexes were prepared by incubating 50ul of supernatants of ɑ-Sxl (DSHB M114) or ɑ-Ubx (DSHB Ubx/ABD-A FP6.87) in binding buffer with 30ul of Protein G beads for 1.5 hours in a total volume of 400ul, washed 2X with binding buffer, 2X with wash buffer (40mM HEPES pH 7.4, 300mM NaCl, 10% glycerol, and 0.2% NP-40), then 2X with binding buffer. .. RNA was treated with DNase I (NEB) and reverse transcribed using Protoscript II (NEB). qPCR was performed on cDNA using SYBR Green I Master Mix (Roche) on a StepOnePlus Real-Time PCR System (Applied Biosciences) using primers listed in .

    Nucleic Acid Electrophoresis:

    Article Title: The role of the bacterial protease Prc in the uropathogenesis of extraintestinal pathogenic Escherichia coli
    Article Snippet: For qPCR, the cDNA and primers were mixed with KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems, Boston, MA, USA) and then subjected to PCR using a StepOnePlus™ Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). .. The resulting products were analyzed by gel electrophoresis and visualized by ethidium bromide (EtBr) staining.

    RNA Sequencing Assay:

    Article Title: Deep evolutionary origin of limb and fin regeneration
    Article Snippet: Polypterus blastemas of 9 dpa and UF were used for RNA extraction and subsequent DNase treatment and purification, performed as described for RNA-seq library preparation. .. Gene-specific oligos for qPCR assays were designed using Primer Express Software v3.0 (Thermo Fisher Scientific) and used in a final concentration of 200 nM to each primer. qPCR was carried out using GoTaq Probe qPCR Master Mix (Promega) in a final volume of 10 μL, in a StepOnePlus Real-Time PCR System (Applied Biosystems), as previously described ( ).

    Fluorescence:

    Article Title: Selective breeding modifies mef2ca mutant incomplete penetrance by tuning the opposing Notch pathway
    Article Snippet: The mef2ca b1086 mutant allele we exclusively use in this study and the KASP genotyping (LGC) protocol for this allele using a StepOnePlus Real-Time PCR System (Applied Biosystems) has been previously described [ , , ]. .. For genotyping dlx5a , heterozygotes and homozygous mutants were discriminated from wild types based on EGFP fluorescence.

    Article Title: Serological and Molecular Identification of Brucella spp. in Pigs from Cairo and Giza Governorates, Egypt
    Article Snippet: .. Amplification and real-time fluorescence detection was carried out on a StepOnePlus™ Real-Time PCR System (Applied Biosystems® , Germany). ..

    Mutagenesis:

    Article Title: Selective breeding modifies mef2ca mutant incomplete penetrance by tuning the opposing Notch pathway
    Article Snippet: .. The mef2ca b1086 mutant allele we exclusively use in this study and the KASP genotyping (LGC) protocol for this allele using a StepOnePlus Real-Time PCR System (Applied Biosystems) has been previously described [ , , ]. ..

    Isolation:

    Article Title: Elevated Intraocular Pressure Causes Abnormal Reactivity of Mouse Retinal Arterioles
    Article Snippet: RNA was isolated using peqGOLD TriFast™ (PEQLAB), and cDNA was generated with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Darmstadt, Germany). .. Quantitative real-time RT-PCR (qPCR) reactions were performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems) using SYBR® Green JumpStart™ Taq ReadyMix™ (Sigma-Aldrich, Munich, Germany) and 20 ng cDNA.

    Article Title: RNF12 catalyzes BRF1 ubiquitination and regulates RNA polymerase III–dependent transcription
    Article Snippet: Total RNA was isolated using TRIzol (Invitrogen). .. Real-time PCR was performed using SYBR premix EX Taq (TaKaRa) and analyzed with the StepOnePlus real-time PCR system (Thermo Fisher Scientific).

    Multiplex Assay:

    Article Title: Serological and Molecular Identification of Brucella spp. in Pigs from Cairo and Giza Governorates, Egypt
    Article Snippet: Briefly, the PCR reaction was done in a 15 μL multiplex PCR mixture with 2× TaqMan™ Environmental master mix (Applied Biosystems® , Germany), 0.2 µM of each primer, 0.1 µM of each probe, and 5 μL of template DNA. .. Amplification and real-time fluorescence detection was carried out on a StepOnePlus™ Real-Time PCR System (Applied Biosystems® , Germany).

    Purification:

    Article Title: The role of the bacterial protease Prc in the uropathogenesis of extraintestinal pathogenic Escherichia coli
    Article Snippet: Real-time quantitative PCR (qPCR) and reverse transcription-PCR (RT-PCR) The purified RNA was reverse transcribed into cDNA by using random hexamer primers and Moloney murine leukemia virus (M-MLV) reverse transcriptase according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA, USA). .. For qPCR, the cDNA and primers were mixed with KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems, Boston, MA, USA) and then subjected to PCR using a StepOnePlus™ Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA).

    Article Title: Deep evolutionary origin of limb and fin regeneration
    Article Snippet: Polypterus blastemas of 9 dpa and UF were used for RNA extraction and subsequent DNase treatment and purification, performed as described for RNA-seq library preparation. .. Gene-specific oligos for qPCR assays were designed using Primer Express Software v3.0 (Thermo Fisher Scientific) and used in a final concentration of 200 nM to each primer. qPCR was carried out using GoTaq Probe qPCR Master Mix (Promega) in a final volume of 10 μL, in a StepOnePlus Real-Time PCR System (Applied Biosystems), as previously described ( ).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The role of the bacterial protease Prc in the uropathogenesis of extraintestinal pathogenic Escherichia coli
    Article Snippet: Paragraph title: Real-time quantitative PCR (qPCR) and reverse transcription-PCR (RT-PCR) ... For qPCR, the cDNA and primers were mixed with KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems, Boston, MA, USA) and then subjected to PCR using a StepOnePlus™ Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA).

    Article Title: RNA‐binding protein NONO promotes breast cancer proliferation by post‐transcriptional regulation of SKP2 and E2F8, et al. RNA‐binding protein NONO promotes breast cancer proliferation by post‐transcriptional regulation of SKP2 and E2F8
    Article Snippet: .. 2.8 Quantitative reverse transcription polymerase chain reaction RNA was extracted from cells using ISOGEN (Nippon Gene) and single‐stranded cDNA was synthesized using Super Script III reverse transcriptase (Invitrogen) with oligo dT or random primers. qRT‐PCR was carried out on StepOnePlus Real‐Time PCR System (Thermo Fisher Scientific) using KAPA SYBR FAST qPCR Kit (KAPA Biosystems) and sets of gene‐specific primers. ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Elevated Intraocular Pressure Causes Abnormal Reactivity of Mouse Retinal Arterioles
    Article Snippet: Quantitative PCR Messenger RNA for the hypoxic markers, hypoxia-inducible factor 1alpha (HIF-1α ) and vascular endothelial growth factor-A (VEGF-A), for the prooxidant redox enzymes, nicotinamide adenine dinucleotide phosphate oxidases 1 and 2 (NOX1 and NOX2), for the antioxidant redox enzymes, catalase (CAT), heme oxygenase 1 (HO-1) and glutathione peroxidases 1 and 4 (GPx1 and GPx4), for the cytokines, tumor necrosis factor alpha (TNF-α ), interferon gamma (IFN-γ ), interleukin 1beta (IL-1β ), and interleukins 2 and 12 (IL-2 and IL-12), and for the nitric oxide synthase (NOS) isoforms, eNOS, iNOS, and nNOS, was quantified in the whole retinal explants as described before [ ]. .. Quantitative real-time RT-PCR (qPCR) reactions were performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems) using SYBR® Green JumpStart™ Taq ReadyMix™ (Sigma-Aldrich, Munich, Germany) and 20 ng cDNA.

    Software:

    Article Title: Deep evolutionary origin of limb and fin regeneration
    Article Snippet: .. Gene-specific oligos for qPCR assays were designed using Primer Express Software v3.0 (Thermo Fisher Scientific) and used in a final concentration of 200 nM to each primer. qPCR was carried out using GoTaq Probe qPCR Master Mix (Promega) in a final volume of 10 μL, in a StepOnePlus Real-Time PCR System (Applied Biosystems), as previously described ( ). ..

    SYBR Green Assay:

    Article Title: Elevated Intraocular Pressure Causes Abnormal Reactivity of Mouse Retinal Arterioles
    Article Snippet: .. Quantitative real-time RT-PCR (qPCR) reactions were performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems) using SYBR® Green JumpStart™ Taq ReadyMix™ (Sigma-Aldrich, Munich, Germany) and 20 ng cDNA. ..

    Article Title: A developmental program truncates long transcripts to temporally regulate cell signaling
    Article Snippet: .. RNA was treated with DNase I (NEB) and reverse transcribed using Protoscript II (NEB). qPCR was performed on cDNA using SYBR Green I Master Mix (Roche) on a StepOnePlus Real-Time PCR System (Applied Biosciences) using primers listed in . .. For long genes sog, NetA, Pka-C3 , and sca , primers used all amplified the 5’ exons of the genes expressed as part of the short forms.

    RNA Extraction:

    Article Title: Deep evolutionary origin of limb and fin regeneration
    Article Snippet: Polypterus blastemas of 9 dpa and UF were used for RNA extraction and subsequent DNase treatment and purification, performed as described for RNA-seq library preparation. .. Gene-specific oligos for qPCR assays were designed using Primer Express Software v3.0 (Thermo Fisher Scientific) and used in a final concentration of 200 nM to each primer. qPCR was carried out using GoTaq Probe qPCR Master Mix (Promega) in a final volume of 10 μL, in a StepOnePlus Real-Time PCR System (Applied Biosystems), as previously described ( ).

    Incubation:

    Article Title: Expression Analysis of the PITX2 Gene and Associations between Its Polymorphisms and Body Size and Carcass Traits in Chickens
    Article Snippet: Real-time PCR was performed using a StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with a 20 μL PCR mixture including 2 μL of cDNA, 10 μL of 2 × SYBR Premix Ex TaqII, 0.4 μL ROX Reference Dye (50×) 0.8 μL of forward primer, 0.8 μL of reverse primer and 6 μL of nuclease-free water. .. Reactions were incubated in a 96-well optical plate (Applied Biosystems, American) at 95 °C for 30 s, followed by 40 cycles of 95 °C for 5 s, and 60 °C for 30 s. Each sample was analyzed in triplicate.

    Article Title: A developmental program truncates long transcripts to temporally regulate cell signaling
    Article Snippet: Proteinase K was added to the eluted material to a final concentration of 1mg/ml and incubated at 50°C for 30 minutes. .. RNA was treated with DNase I (NEB) and reverse transcribed using Protoscript II (NEB). qPCR was performed on cDNA using SYBR Green I Master Mix (Roche) on a StepOnePlus Real-Time PCR System (Applied Biosciences) using primers listed in .

    Spectrophotometry:

    Article Title: Knockout of beta‐2 microglobulin reduces stem cell‐induced immune rejection and enhances ischaemic hindlimb repair via exosome/miR‐24/Bim pathway, et al. Knockout of beta‐2 microglobulin reduces stem cell‐induced immune rejection and enhances ischaemic hindlimb repair via exosome/miR‐24/Bim pathway
    Article Snippet: RNA concentrations were measured using a spectrophotometer (NanoDrop). .. The expression levels of miRNAs were analysed by qRT‐PCR using Takara SYBR Premix Ex Taq (Tli RNaseH Plus) in a StepOnePlus Real‐Time PCR system (Applied Biosystems).

    Concentration Assay:

    Article Title: A developmental program truncates long transcripts to temporally regulate cell signaling
    Article Snippet: Proteinase K was added to the eluted material to a final concentration of 1mg/ml and incubated at 50°C for 30 minutes. .. RNA was treated with DNase I (NEB) and reverse transcribed using Protoscript II (NEB). qPCR was performed on cDNA using SYBR Green I Master Mix (Roche) on a StepOnePlus Real-Time PCR System (Applied Biosciences) using primers listed in .

    Article Title: The effects of transfer from steady-state to tidally-changing salinities on plasma and branchial osmoregulatory variables in adult Mozambique tilapia.
    Article Snippet: Quantitative real-time PCRs (qRT-PCRs) were set up as previously described , using the StepOnePlus real-time PCR system (Applied Biosystems). .. Standard curves for quantification were generated using serially diluted target gene cDNA fragments of known concentration (standard cDNAs).

    Article Title: Deep evolutionary origin of limb and fin regeneration
    Article Snippet: .. Gene-specific oligos for qPCR assays were designed using Primer Express Software v3.0 (Thermo Fisher Scientific) and used in a final concentration of 200 nM to each primer. qPCR was carried out using GoTaq Probe qPCR Master Mix (Promega) in a final volume of 10 μL, in a StepOnePlus Real-Time PCR System (Applied Biosystems), as previously described ( ). ..

    Staining:

    Article Title: The role of the bacterial protease Prc in the uropathogenesis of extraintestinal pathogenic Escherichia coli
    Article Snippet: For qPCR, the cDNA and primers were mixed with KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems, Boston, MA, USA) and then subjected to PCR using a StepOnePlus™ Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). .. The resulting products were analyzed by gel electrophoresis and visualized by ethidium bromide (EtBr) staining.

    Fluorescence In Situ Hybridization:

    Article Title: Selective breeding modifies mef2ca mutant incomplete penetrance by tuning the opposing Notch pathway
    Article Snippet: Zebrafish strains and husbandry All fish were maintained and staged according to established protocols [ , ]. .. The mef2ca b1086 mutant allele we exclusively use in this study and the KASP genotyping (LGC) protocol for this allele using a StepOnePlus Real-Time PCR System (Applied Biosystems) has been previously described [ , , ].

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  • 99
    Thermo Fisher real time pcr system
    Genotyping assay for the detection of the mutant allele (with the BRCA1 ex9-12del mutation) and the wild-type allele (exon 11) by real-time <t>PCR</t> and <t>TaqMan</t> ® probes. The AD plot shows 2 groups; green color samples have the allele with the deletion, samples in red color possess exon 11 or the wild-type allele (in this group all the samples are present, the 6 negative and the 4 positive ones). Samples in blue color are negative samples for the deletion, which have no fluorescence and are classified by the software as negatives. Negative controls are shown in black squares. Each sample was processed by duplicate. The axis values represent relative fluorescence (∆Rn) between both dyes (FAM ™ for the mutant allele and HEX ™ for the wild-type allele). PCR, polymerase chain reaction; AD, allelic discrimination.
    Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2976 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr system/product/Thermo Fisher
    Average 99 stars, based on 2976 article reviews
    Price from $9.99 to $1999.99
    real time pcr system - by Bioz Stars, 2020-04
    99/100 stars
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    99
    Thermo Fisher steponeplus real time system
    p21-/- attenuates the antiproliferative effects of AzaC (a) p21 mRNA is significantly upregulated in CD4+ and CD8+ Teff following treatment with AzaC. Teffs were isolated from the spleens of B6. Foxp3 GFP × B6.CAG DSRED and nTregs were isolated from B6. Foxp3 GFP . Cells were co-cultured at a 1:10 ratio of nTregs to Teffs for 2 days in the presence of anti-CD3/CD28 beads (bead:cell 1:1; Invitrogen) and Xcyte medium supplemented with L-glutamine (4 mM), penicillin (100 U/mL), streptomycin (100 μg/mL), and human recombinant IL-2 (hIL-2; 500 U/mL). The activated T cells were cultured with AzaC (1 μM) or PBS for an additional 2 days. Cells were sorted using FACS Aria II (BD) to isolate nTregs (CD4+DSRED-FOXP3GFP+), CD4+ Teffs (CD4+DSRed+FOXP3GFP-), and CD8+ Teffs (CD8+DSRed+FOXP3GFP-) prior to RNA extraction. QPCR was performed on the Applied Biosystems <t>StepOnePlus</t> Real-Time System using pre-designed TaqMan® Gene Expression Assays (18S RNA Mm03928990 and p21 Mm04205640). Relative fold changes in expression were determined using the ΔΔCT method. AzaC treatment resulted in a 3.4 fold increase of p21 expression in CD4+ Teffs (FACS sorted to remove AzaC converted Tregs) (AzaC vs. PBS p
    Steponeplus Real Time System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3228 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher applied biosystemstm thermo fisher scientific steponeplustm real time pcr system
    p21-/- attenuates the antiproliferative effects of AzaC (a) p21 mRNA is significantly upregulated in CD4+ and CD8+ Teff following treatment with AzaC. Teffs were isolated from the spleens of B6. Foxp3 GFP × B6.CAG DSRED and nTregs were isolated from B6. Foxp3 GFP . Cells were co-cultured at a 1:10 ratio of nTregs to Teffs for 2 days in the presence of anti-CD3/CD28 beads (bead:cell 1:1; Invitrogen) and Xcyte medium supplemented with L-glutamine (4 mM), penicillin (100 U/mL), streptomycin (100 μg/mL), and human recombinant IL-2 (hIL-2; 500 U/mL). The activated T cells were cultured with AzaC (1 μM) or PBS for an additional 2 days. Cells were sorted using FACS Aria II (BD) to isolate nTregs (CD4+DSRED-FOXP3GFP+), CD4+ Teffs (CD4+DSRed+FOXP3GFP-), and CD8+ Teffs (CD8+DSRed+FOXP3GFP-) prior to RNA extraction. QPCR was performed on the Applied Biosystems <t>StepOnePlus</t> Real-Time System using pre-designed TaqMan® Gene Expression Assays (18S RNA Mm03928990 and p21 Mm04205640). Relative fold changes in expression were determined using the ΔΔCT method. AzaC treatment resulted in a 3.4 fold increase of p21 expression in CD4+ Teffs (FACS sorted to remove AzaC converted Tregs) (AzaC vs. PBS p
    Applied Biosystemstm Thermo Fisher Scientific Steponeplustm Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/applied biosystemstm thermo fisher scientific steponeplustm real time pcr system/product/Thermo Fisher
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    Image Search Results


    Genotyping assay for the detection of the mutant allele (with the BRCA1 ex9-12del mutation) and the wild-type allele (exon 11) by real-time PCR and TaqMan ® probes. The AD plot shows 2 groups; green color samples have the allele with the deletion, samples in red color possess exon 11 or the wild-type allele (in this group all the samples are present, the 6 negative and the 4 positive ones). Samples in blue color are negative samples for the deletion, which have no fluorescence and are classified by the software as negatives. Negative controls are shown in black squares. Each sample was processed by duplicate. The axis values represent relative fluorescence (∆Rn) between both dyes (FAM ™ for the mutant allele and HEX ™ for the wild-type allele). PCR, polymerase chain reaction; AD, allelic discrimination.

    Journal: Molecular Medicine Reports

    Article Title: A novel method to detect the Mexican founder mutation BRCA1 ex9-12del associated with breast and ovarian cancer using quantitative polymerase chain reaction and TaqMan® probes

    doi: 10.3892/mmr.2018.9141

    Figure Lengend Snippet: Genotyping assay for the detection of the mutant allele (with the BRCA1 ex9-12del mutation) and the wild-type allele (exon 11) by real-time PCR and TaqMan ® probes. The AD plot shows 2 groups; green color samples have the allele with the deletion, samples in red color possess exon 11 or the wild-type allele (in this group all the samples are present, the 6 negative and the 4 positive ones). Samples in blue color are negative samples for the deletion, which have no fluorescence and are classified by the software as negatives. Negative controls are shown in black squares. Each sample was processed by duplicate. The axis values represent relative fluorescence (∆Rn) between both dyes (FAM ™ for the mutant allele and HEX ™ for the wild-type allele). PCR, polymerase chain reaction; AD, allelic discrimination.

    Article Snippet: qPCR with designed primers and TaqMan® probes Thermocycler StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific, Inc.) was used to perform the qPCR with TaqMan® probes.

    Techniques: Genotyping Assay, Mutagenesis, Real-time Polymerase Chain Reaction, Fluorescence, Software, Polymerase Chain Reaction

    Detection of the BRCA1 ex9-12del mutation by real-time PCR and TaqMan ® probe. Red amplification curves represent the fluorescent signal given by the FAM ™ dye from the TaqMan ® probe detecting the variant allele in the four positive samples, which had a mean Ct of 27.46. No amplification curve is observed for the negative samples neither for negative controls (horizontal red lines). The ‘Y’ axis values represent the fluorescent signal normalized of the FAM ™ dye with the ROX, whereas the ‘X’ axis represent number of cycles. PCR. PCR, polymerase chain reaction; ROX, passive reference dye.

    Journal: Molecular Medicine Reports

    Article Title: A novel method to detect the Mexican founder mutation BRCA1 ex9-12del associated with breast and ovarian cancer using quantitative polymerase chain reaction and TaqMan® probes

    doi: 10.3892/mmr.2018.9141

    Figure Lengend Snippet: Detection of the BRCA1 ex9-12del mutation by real-time PCR and TaqMan ® probe. Red amplification curves represent the fluorescent signal given by the FAM ™ dye from the TaqMan ® probe detecting the variant allele in the four positive samples, which had a mean Ct of 27.46. No amplification curve is observed for the negative samples neither for negative controls (horizontal red lines). The ‘Y’ axis values represent the fluorescent signal normalized of the FAM ™ dye with the ROX, whereas the ‘X’ axis represent number of cycles. PCR. PCR, polymerase chain reaction; ROX, passive reference dye.

    Article Snippet: qPCR with designed primers and TaqMan® probes Thermocycler StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific, Inc.) was used to perform the qPCR with TaqMan® probes.

    Techniques: Mutagenesis, Real-time Polymerase Chain Reaction, Amplification, Variant Assay, Polymerase Chain Reaction

    p21-/- attenuates the antiproliferative effects of AzaC (a) p21 mRNA is significantly upregulated in CD4+ and CD8+ Teff following treatment with AzaC. Teffs were isolated from the spleens of B6. Foxp3 GFP × B6.CAG DSRED and nTregs were isolated from B6. Foxp3 GFP . Cells were co-cultured at a 1:10 ratio of nTregs to Teffs for 2 days in the presence of anti-CD3/CD28 beads (bead:cell 1:1; Invitrogen) and Xcyte medium supplemented with L-glutamine (4 mM), penicillin (100 U/mL), streptomycin (100 μg/mL), and human recombinant IL-2 (hIL-2; 500 U/mL). The activated T cells were cultured with AzaC (1 μM) or PBS for an additional 2 days. Cells were sorted using FACS Aria II (BD) to isolate nTregs (CD4+DSRED-FOXP3GFP+), CD4+ Teffs (CD4+DSRed+FOXP3GFP-), and CD8+ Teffs (CD8+DSRed+FOXP3GFP-) prior to RNA extraction. QPCR was performed on the Applied Biosystems StepOnePlus Real-Time System using pre-designed TaqMan® Gene Expression Assays (18S RNA Mm03928990 and p21 Mm04205640). Relative fold changes in expression were determined using the ΔΔCT method. AzaC treatment resulted in a 3.4 fold increase of p21 expression in CD4+ Teffs (FACS sorted to remove AzaC converted Tregs) (AzaC vs. PBS p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Azacitidine mitigates GvHD via differential effects on the proliferation of T effectors and nTregs in vivo

    doi: 10.4049/jimmunol.1502399

    Figure Lengend Snippet: p21-/- attenuates the antiproliferative effects of AzaC (a) p21 mRNA is significantly upregulated in CD4+ and CD8+ Teff following treatment with AzaC. Teffs were isolated from the spleens of B6. Foxp3 GFP × B6.CAG DSRED and nTregs were isolated from B6. Foxp3 GFP . Cells were co-cultured at a 1:10 ratio of nTregs to Teffs for 2 days in the presence of anti-CD3/CD28 beads (bead:cell 1:1; Invitrogen) and Xcyte medium supplemented with L-glutamine (4 mM), penicillin (100 U/mL), streptomycin (100 μg/mL), and human recombinant IL-2 (hIL-2; 500 U/mL). The activated T cells were cultured with AzaC (1 μM) or PBS for an additional 2 days. Cells were sorted using FACS Aria II (BD) to isolate nTregs (CD4+DSRED-FOXP3GFP+), CD4+ Teffs (CD4+DSRed+FOXP3GFP-), and CD8+ Teffs (CD8+DSRed+FOXP3GFP-) prior to RNA extraction. QPCR was performed on the Applied Biosystems StepOnePlus Real-Time System using pre-designed TaqMan® Gene Expression Assays (18S RNA Mm03928990 and p21 Mm04205640). Relative fold changes in expression were determined using the ΔΔCT method. AzaC treatment resulted in a 3.4 fold increase of p21 expression in CD4+ Teffs (FACS sorted to remove AzaC converted Tregs) (AzaC vs. PBS p

    Article Snippet: QPCR was performed on the Applied Biosystems StepOnePlus Real-Time System (Thermo fisher) using pre-designed TaqMan® Gene Expression Assays (Life Technologies) (18S RNA Mm03928990 and p21 Mm04205640) according to manufacturer's instructions.

    Techniques: Isolation, Cell Culture, Recombinant, FACS, RNA Extraction, Real-time Polymerase Chain Reaction, Expressing