steponeplus instrument  (Thermo Fisher)


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    Structured Review

    Thermo Fisher steponeplus instrument
    Steponeplus Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/steponeplus instrument/product/Thermo Fisher
    Average 99 stars, based on 199 article reviews
    Price from $9.99 to $1999.99
    steponeplus instrument - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Human Metapneumovirus Infection Inhibits Cathelicidin Antimicrobial Peptide Expression in Human Macrophages
    Article Snippet: .. Quantitative Real-Time PCR (qRT-PCR) RNA was isolated with the RNeasy mini kit (Qiagen) following the manufacturer’s protocol. cDNA was synthesized from isolated RNA using the qScript kit (Quanta) following the manufacturer’s protocol. qRT-PCR was performed using Perfecta SYBR Green reaction mix (Quanta) and a StepOnePlus instrument (Life Technologies) with the temperature profile at 95°C for 20 s, 40 cycles at 95°C for 3 s, and at 60°C for 30 s. Fold change in gene expression was calculated using the ΔΔCt-method normalized against GAPDH. ..

    Article Title: Anti-inflammatory effect of oligostilbenoids from Vitis heyneana in LPS-stimulated RAW 264.7 macrophages via suppressing the NF-κB activation
    Article Snippet: .. COX-2 mRNA expression was analyzed by real-time qPCR (StepOnePlus qPCR cycler, Applied Biosystems) using QuantiFast SYBR Green RT-PCR Kit Qiagen, (#204156) and primers (Qiagen): COX-2 (Ptgs2) (Cat: QT00165347), Mm_GADPH (Cat: QT01658692). ..

    Article Title: Self-powered RNA nanomachine driven by metastable structure
    Article Snippet: .. Broccoli-DFHBI-1T fluorescence of these samples were monitored at 37°C using a real-time PCR instrument (StepOnePlus, Applied Biosystems). ..

    Article Title: Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells
    Article Snippet: .. Real-Time PCR was performed using TaqMan gene expression assays (Applied Biosystems) and Real-Time PCR machine (StepOnePlus, Applied Biosystems). .. The genes include differentiation markers (as mentioned earlier), stemness markers such as OCT-4 (Hs04260367_g1), REX-1 (Hs01938187_s1), SOX-2 (Hs01053049_s1) and NANOG (Hs01060663_m1).

    Article Title: Drosophila insulin-like peptide 1 (DILP1) is transiently expressed during non-feeding stages and reproductive dormancy
    Article Snippet: .. The cDNA was then applied for quantitative real-time PCR (qPCR) using a StepOnePlus™ System (Applied Biosystem, USA) instrument and SensiFAST SYBR Hi-ROX Kit (Bioline) according to the manufacturer. .. For each sample triplicate reactions of the total volume of 20 μl were conducted with a primer concentration of 400 nM and 4 μl of diluted 1:10 cDNA template.

    Article Title: Deciphering the rules by which 5?-UTR sequences affect protein expression in yeast
    Article Snippet: .. Quantitative PCR (qPCR) was performed by using Applied Biosystems real-time PCR instrument (StepOnePlus). qPCR primers were designed to yield YFP (the target) and mCherry (the endogenous control) products of 106- and 135- bp, respectively (for primers, see ). ..

    Article Title: Adaptive Natural Killer Cells Integrate Interleukin-18 during Target-Cell Encounter
    Article Snippet: .. Quantitative RT-PCR To determine transcript levels, 50 ng of total RNA was reverse transcribed using TaqMan Reverse Transcription Reagents (Applied Biosciences), and quantitative real-time PCR was performed with TaqMan gene expression assays (Table S2 in Supplementary Material) on a StepOnePlus instrument (Applied Biosciences). .. Signaling Assays To investigate phosphorylation of NF-κB and STAT4, CD56+ MACS-enriched cells were thawed, FACS-purified for viable CD3− CD56dim CD57+ NKG2C+ adaptive NK cells, CD56dim CD57− immature NK cells, or CD56dim CD57+ mature NK cells, and rested overnight in complete medium.

    Article Title: A Symphytum officinale Root Extract Exerts Anti-inflammatory Properties by Affecting Two Distinct Steps of NF-κB Signaling
    Article Snippet: .. Real-time PCR was done with the SsoAdvanced Universal SYBR Green Supermix (BioRad) using the StepOnePlus instrument (Applied Biosystems), and relative mRNA expression normalized to GAPDH. .. Fold changes in mRNA expression were calculated according to the 2-ΔΔCT method.

    Fluorescence:

    Article Title: Self-powered RNA nanomachine driven by metastable structure
    Article Snippet: .. Broccoli-DFHBI-1T fluorescence of these samples were monitored at 37°C using a real-time PCR instrument (StepOnePlus, Applied Biosystems). ..

    Synthesized:

    Article Title: Human Metapneumovirus Infection Inhibits Cathelicidin Antimicrobial Peptide Expression in Human Macrophages
    Article Snippet: .. Quantitative Real-Time PCR (qRT-PCR) RNA was isolated with the RNeasy mini kit (Qiagen) following the manufacturer’s protocol. cDNA was synthesized from isolated RNA using the qScript kit (Quanta) following the manufacturer’s protocol. qRT-PCR was performed using Perfecta SYBR Green reaction mix (Quanta) and a StepOnePlus instrument (Life Technologies) with the temperature profile at 95°C for 20 s, 40 cycles at 95°C for 3 s, and at 60°C for 30 s. Fold change in gene expression was calculated using the ΔΔCt-method normalized against GAPDH. ..

    Isolation:

    Article Title: Human Metapneumovirus Infection Inhibits Cathelicidin Antimicrobial Peptide Expression in Human Macrophages
    Article Snippet: .. Quantitative Real-Time PCR (qRT-PCR) RNA was isolated with the RNeasy mini kit (Qiagen) following the manufacturer’s protocol. cDNA was synthesized from isolated RNA using the qScript kit (Quanta) following the manufacturer’s protocol. qRT-PCR was performed using Perfecta SYBR Green reaction mix (Quanta) and a StepOnePlus instrument (Life Technologies) with the temperature profile at 95°C for 20 s, 40 cycles at 95°C for 3 s, and at 60°C for 30 s. Fold change in gene expression was calculated using the ΔΔCt-method normalized against GAPDH. ..

    Quantitative RT-PCR:

    Article Title: Human Metapneumovirus Infection Inhibits Cathelicidin Antimicrobial Peptide Expression in Human Macrophages
    Article Snippet: .. Quantitative Real-Time PCR (qRT-PCR) RNA was isolated with the RNeasy mini kit (Qiagen) following the manufacturer’s protocol. cDNA was synthesized from isolated RNA using the qScript kit (Quanta) following the manufacturer’s protocol. qRT-PCR was performed using Perfecta SYBR Green reaction mix (Quanta) and a StepOnePlus instrument (Life Technologies) with the temperature profile at 95°C for 20 s, 40 cycles at 95°C for 3 s, and at 60°C for 30 s. Fold change in gene expression was calculated using the ΔΔCt-method normalized against GAPDH. ..

    Article Title: Adaptive Natural Killer Cells Integrate Interleukin-18 during Target-Cell Encounter
    Article Snippet: .. Quantitative RT-PCR To determine transcript levels, 50 ng of total RNA was reverse transcribed using TaqMan Reverse Transcription Reagents (Applied Biosciences), and quantitative real-time PCR was performed with TaqMan gene expression assays (Table S2 in Supplementary Material) on a StepOnePlus instrument (Applied Biosciences). .. Signaling Assays To investigate phosphorylation of NF-κB and STAT4, CD56+ MACS-enriched cells were thawed, FACS-purified for viable CD3− CD56dim CD57+ NKG2C+ adaptive NK cells, CD56dim CD57− immature NK cells, or CD56dim CD57+ mature NK cells, and rested overnight in complete medium.

    SYBR Green Assay:

    Article Title: Human Metapneumovirus Infection Inhibits Cathelicidin Antimicrobial Peptide Expression in Human Macrophages
    Article Snippet: .. Quantitative Real-Time PCR (qRT-PCR) RNA was isolated with the RNeasy mini kit (Qiagen) following the manufacturer’s protocol. cDNA was synthesized from isolated RNA using the qScript kit (Quanta) following the manufacturer’s protocol. qRT-PCR was performed using Perfecta SYBR Green reaction mix (Quanta) and a StepOnePlus instrument (Life Technologies) with the temperature profile at 95°C for 20 s, 40 cycles at 95°C for 3 s, and at 60°C for 30 s. Fold change in gene expression was calculated using the ΔΔCt-method normalized against GAPDH. ..

    Article Title: Anti-inflammatory effect of oligostilbenoids from Vitis heyneana in LPS-stimulated RAW 264.7 macrophages via suppressing the NF-κB activation
    Article Snippet: .. COX-2 mRNA expression was analyzed by real-time qPCR (StepOnePlus qPCR cycler, Applied Biosystems) using QuantiFast SYBR Green RT-PCR Kit Qiagen, (#204156) and primers (Qiagen): COX-2 (Ptgs2) (Cat: QT00165347), Mm_GADPH (Cat: QT01658692). ..

    Article Title: A Symphytum officinale Root Extract Exerts Anti-inflammatory Properties by Affecting Two Distinct Steps of NF-κB Signaling
    Article Snippet: .. Real-time PCR was done with the SsoAdvanced Universal SYBR Green Supermix (BioRad) using the StepOnePlus instrument (Applied Biosystems), and relative mRNA expression normalized to GAPDH. .. Fold changes in mRNA expression were calculated according to the 2-ΔΔCT method.

    Expressing:

    Article Title: Human Metapneumovirus Infection Inhibits Cathelicidin Antimicrobial Peptide Expression in Human Macrophages
    Article Snippet: .. Quantitative Real-Time PCR (qRT-PCR) RNA was isolated with the RNeasy mini kit (Qiagen) following the manufacturer’s protocol. cDNA was synthesized from isolated RNA using the qScript kit (Quanta) following the manufacturer’s protocol. qRT-PCR was performed using Perfecta SYBR Green reaction mix (Quanta) and a StepOnePlus instrument (Life Technologies) with the temperature profile at 95°C for 20 s, 40 cycles at 95°C for 3 s, and at 60°C for 30 s. Fold change in gene expression was calculated using the ΔΔCt-method normalized against GAPDH. ..

    Article Title: Anti-inflammatory effect of oligostilbenoids from Vitis heyneana in LPS-stimulated RAW 264.7 macrophages via suppressing the NF-κB activation
    Article Snippet: .. COX-2 mRNA expression was analyzed by real-time qPCR (StepOnePlus qPCR cycler, Applied Biosystems) using QuantiFast SYBR Green RT-PCR Kit Qiagen, (#204156) and primers (Qiagen): COX-2 (Ptgs2) (Cat: QT00165347), Mm_GADPH (Cat: QT01658692). ..

    Article Title: Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells
    Article Snippet: .. Real-Time PCR was performed using TaqMan gene expression assays (Applied Biosystems) and Real-Time PCR machine (StepOnePlus, Applied Biosystems). .. The genes include differentiation markers (as mentioned earlier), stemness markers such as OCT-4 (Hs04260367_g1), REX-1 (Hs01938187_s1), SOX-2 (Hs01053049_s1) and NANOG (Hs01060663_m1).

    Article Title: Adaptive Natural Killer Cells Integrate Interleukin-18 during Target-Cell Encounter
    Article Snippet: .. Quantitative RT-PCR To determine transcript levels, 50 ng of total RNA was reverse transcribed using TaqMan Reverse Transcription Reagents (Applied Biosciences), and quantitative real-time PCR was performed with TaqMan gene expression assays (Table S2 in Supplementary Material) on a StepOnePlus instrument (Applied Biosciences). .. Signaling Assays To investigate phosphorylation of NF-κB and STAT4, CD56+ MACS-enriched cells were thawed, FACS-purified for viable CD3− CD56dim CD57+ NKG2C+ adaptive NK cells, CD56dim CD57− immature NK cells, or CD56dim CD57+ mature NK cells, and rested overnight in complete medium.

    Article Title: A Symphytum officinale Root Extract Exerts Anti-inflammatory Properties by Affecting Two Distinct Steps of NF-κB Signaling
    Article Snippet: .. Real-time PCR was done with the SsoAdvanced Universal SYBR Green Supermix (BioRad) using the StepOnePlus instrument (Applied Biosystems), and relative mRNA expression normalized to GAPDH. .. Fold changes in mRNA expression were calculated according to the 2-ΔΔCT method.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Anti-inflammatory effect of oligostilbenoids from Vitis heyneana in LPS-stimulated RAW 264.7 macrophages via suppressing the NF-κB activation
    Article Snippet: .. COX-2 mRNA expression was analyzed by real-time qPCR (StepOnePlus qPCR cycler, Applied Biosystems) using QuantiFast SYBR Green RT-PCR Kit Qiagen, (#204156) and primers (Qiagen): COX-2 (Ptgs2) (Cat: QT00165347), Mm_GADPH (Cat: QT01658692). ..

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    Thermo Fisher real time pcr system
    Genotyping assay for the detection of the mutant allele (with the BRCA1 ex9-12del mutation) and the wild-type allele (exon 11) by real-time <t>PCR</t> and <t>TaqMan</t> ® probes. The AD plot shows 2 groups; green color samples have the allele with the deletion, samples in red color possess exon 11 or the wild-type allele (in this group all the samples are present, the 6 negative and the 4 positive ones). Samples in blue color are negative samples for the deletion, which have no fluorescence and are classified by the software as negatives. Negative controls are shown in black squares. Each sample was processed by duplicate. The axis values represent relative fluorescence (∆Rn) between both dyes (FAM ™ for the mutant allele and HEX ™ for the wild-type allele). PCR, polymerase chain reaction; AD, allelic discrimination.
    Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1692 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr system/product/Thermo Fisher
    Average 99 stars, based on 1692 article reviews
    Price from $9.99 to $1999.99
    real time pcr system - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    88
    Thermo Fisher steponeplus device
    Genotyping assay for the detection of the mutant allele (with the BRCA1 ex9-12del mutation) and the wild-type allele (exon 11) by real-time <t>PCR</t> and <t>TaqMan</t> ® probes. The AD plot shows 2 groups; green color samples have the allele with the deletion, samples in red color possess exon 11 or the wild-type allele (in this group all the samples are present, the 6 negative and the 4 positive ones). Samples in blue color are negative samples for the deletion, which have no fluorescence and are classified by the software as negatives. Negative controls are shown in black squares. Each sample was processed by duplicate. The axis values represent relative fluorescence (∆Rn) between both dyes (FAM ™ for the mutant allele and HEX ™ for the wild-type allele). PCR, polymerase chain reaction; AD, allelic discrimination.
    Steponeplus Device, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/steponeplus device/product/Thermo Fisher
    Average 88 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    steponeplus device - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    94
    Thermo Fisher steponeplus instrument
    Genotyping assay for the detection of the mutant allele (with the BRCA1 ex9-12del mutation) and the wild-type allele (exon 11) by real-time <t>PCR</t> and <t>TaqMan</t> ® probes. The AD plot shows 2 groups; green color samples have the allele with the deletion, samples in red color possess exon 11 or the wild-type allele (in this group all the samples are present, the 6 negative and the 4 positive ones). Samples in blue color are negative samples for the deletion, which have no fluorescence and are classified by the software as negatives. Negative controls are shown in black squares. Each sample was processed by duplicate. The axis values represent relative fluorescence (∆Rn) between both dyes (FAM ™ for the mutant allele and HEX ™ for the wild-type allele). PCR, polymerase chain reaction; AD, allelic discrimination.
    Steponeplus Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/steponeplus instrument/product/Thermo Fisher
    Average 94 stars, based on 199 article reviews
    Price from $9.99 to $1999.99
    steponeplus instrument - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    Genotyping assay for the detection of the mutant allele (with the BRCA1 ex9-12del mutation) and the wild-type allele (exon 11) by real-time PCR and TaqMan ® probes. The AD plot shows 2 groups; green color samples have the allele with the deletion, samples in red color possess exon 11 or the wild-type allele (in this group all the samples are present, the 6 negative and the 4 positive ones). Samples in blue color are negative samples for the deletion, which have no fluorescence and are classified by the software as negatives. Negative controls are shown in black squares. Each sample was processed by duplicate. The axis values represent relative fluorescence (∆Rn) between both dyes (FAM ™ for the mutant allele and HEX ™ for the wild-type allele). PCR, polymerase chain reaction; AD, allelic discrimination.

    Journal: Molecular Medicine Reports

    Article Title: A novel method to detect the Mexican founder mutation BRCA1 ex9-12del associated with breast and ovarian cancer using quantitative polymerase chain reaction and TaqMan® probes

    doi: 10.3892/mmr.2018.9141

    Figure Lengend Snippet: Genotyping assay for the detection of the mutant allele (with the BRCA1 ex9-12del mutation) and the wild-type allele (exon 11) by real-time PCR and TaqMan ® probes. The AD plot shows 2 groups; green color samples have the allele with the deletion, samples in red color possess exon 11 or the wild-type allele (in this group all the samples are present, the 6 negative and the 4 positive ones). Samples in blue color are negative samples for the deletion, which have no fluorescence and are classified by the software as negatives. Negative controls are shown in black squares. Each sample was processed by duplicate. The axis values represent relative fluorescence (∆Rn) between both dyes (FAM ™ for the mutant allele and HEX ™ for the wild-type allele). PCR, polymerase chain reaction; AD, allelic discrimination.

    Article Snippet: qPCR with designed primers and TaqMan® probes Thermocycler StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific, Inc.) was used to perform the qPCR with TaqMan® probes.

    Techniques: Genotyping Assay, Mutagenesis, Real-time Polymerase Chain Reaction, Fluorescence, Software, Polymerase Chain Reaction

    Detection of the BRCA1 ex9-12del mutation by real-time PCR and TaqMan ® probe. Red amplification curves represent the fluorescent signal given by the FAM ™ dye from the TaqMan ® probe detecting the variant allele in the four positive samples, which had a mean Ct of 27.46. No amplification curve is observed for the negative samples neither for negative controls (horizontal red lines). The ‘Y’ axis values represent the fluorescent signal normalized of the FAM ™ dye with the ROX, whereas the ‘X’ axis represent number of cycles. PCR. PCR, polymerase chain reaction; ROX, passive reference dye.

    Journal: Molecular Medicine Reports

    Article Title: A novel method to detect the Mexican founder mutation BRCA1 ex9-12del associated with breast and ovarian cancer using quantitative polymerase chain reaction and TaqMan® probes

    doi: 10.3892/mmr.2018.9141

    Figure Lengend Snippet: Detection of the BRCA1 ex9-12del mutation by real-time PCR and TaqMan ® probe. Red amplification curves represent the fluorescent signal given by the FAM ™ dye from the TaqMan ® probe detecting the variant allele in the four positive samples, which had a mean Ct of 27.46. No amplification curve is observed for the negative samples neither for negative controls (horizontal red lines). The ‘Y’ axis values represent the fluorescent signal normalized of the FAM ™ dye with the ROX, whereas the ‘X’ axis represent number of cycles. PCR. PCR, polymerase chain reaction; ROX, passive reference dye.

    Article Snippet: qPCR with designed primers and TaqMan® probes Thermocycler StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific, Inc.) was used to perform the qPCR with TaqMan® probes.

    Techniques: Mutagenesis, Real-time Polymerase Chain Reaction, Amplification, Variant Assay, Polymerase Chain Reaction

    p21-/- attenuates the antiproliferative effects of AzaC (a) p21 mRNA is significantly upregulated in CD4+ and CD8+ Teff following treatment with AzaC. Teffs were isolated from the spleens of B6. Foxp3 GFP × B6.CAG DSRED and nTregs were isolated from B6. Foxp3 GFP . Cells were co-cultured at a 1:10 ratio of nTregs to Teffs for 2 days in the presence of anti-CD3/CD28 beads (bead:cell 1:1; Invitrogen) and Xcyte medium supplemented with L-glutamine (4 mM), penicillin (100 U/mL), streptomycin (100 μg/mL), and human recombinant IL-2 (hIL-2; 500 U/mL). The activated T cells were cultured with AzaC (1 μM) or PBS for an additional 2 days. Cells were sorted using FACS Aria II (BD) to isolate nTregs (CD4+DSRED-FOXP3GFP+), CD4+ Teffs (CD4+DSRed+FOXP3GFP-), and CD8+ Teffs (CD8+DSRed+FOXP3GFP-) prior to RNA extraction. QPCR was performed on the Applied Biosystems StepOnePlus Real-Time System using pre-designed TaqMan® Gene Expression Assays (18S RNA Mm03928990 and p21 Mm04205640). Relative fold changes in expression were determined using the ΔΔCT method. AzaC treatment resulted in a 3.4 fold increase of p21 expression in CD4+ Teffs (FACS sorted to remove AzaC converted Tregs) (AzaC vs. PBS p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Azacitidine mitigates GvHD via differential effects on the proliferation of T effectors and nTregs in vivo

    doi: 10.4049/jimmunol.1502399

    Figure Lengend Snippet: p21-/- attenuates the antiproliferative effects of AzaC (a) p21 mRNA is significantly upregulated in CD4+ and CD8+ Teff following treatment with AzaC. Teffs were isolated from the spleens of B6. Foxp3 GFP × B6.CAG DSRED and nTregs were isolated from B6. Foxp3 GFP . Cells were co-cultured at a 1:10 ratio of nTregs to Teffs for 2 days in the presence of anti-CD3/CD28 beads (bead:cell 1:1; Invitrogen) and Xcyte medium supplemented with L-glutamine (4 mM), penicillin (100 U/mL), streptomycin (100 μg/mL), and human recombinant IL-2 (hIL-2; 500 U/mL). The activated T cells were cultured with AzaC (1 μM) or PBS for an additional 2 days. Cells were sorted using FACS Aria II (BD) to isolate nTregs (CD4+DSRED-FOXP3GFP+), CD4+ Teffs (CD4+DSRed+FOXP3GFP-), and CD8+ Teffs (CD8+DSRed+FOXP3GFP-) prior to RNA extraction. QPCR was performed on the Applied Biosystems StepOnePlus Real-Time System using pre-designed TaqMan® Gene Expression Assays (18S RNA Mm03928990 and p21 Mm04205640). Relative fold changes in expression were determined using the ΔΔCT method. AzaC treatment resulted in a 3.4 fold increase of p21 expression in CD4+ Teffs (FACS sorted to remove AzaC converted Tregs) (AzaC vs. PBS p

    Article Snippet: QPCR was performed on the Applied Biosystems StepOnePlus Real-Time System (Thermo fisher) using pre-designed TaqMan® Gene Expression Assays (Life Technologies) (18S RNA Mm03928990 and p21 Mm04205640) according to manufacturer's instructions.

    Techniques: Isolation, Cell Culture, Recombinant, FACS, RNA Extraction, Real-time Polymerase Chain Reaction, Expressing