stepone real time polymerase chain reaction system  (Thermo Fisher)


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    Name:
    StepOne Real Time PCR System
    Description:
    The StepOne Real Time PCR System is a 48 well low throughput Real Time PCR instrument perfect for both first time and experienced users The StepOne Real Time PCR System can be setup in a variety of configurations and comes ready to use out of the box with intuitive data analysis and instrument control software Utilizing robust LED based 3 color optical recording the StepOne Real Time PCR System is designed to deliver precise quantitative Real Time PCR results for a variety of genomic research applications Features of the The StepOne Real Time PCR System include • Advanced software and instrumentation for performing a wide array of genomic assays • Sensitive 3 color optical LED recording system• Intuitive and robust software perfect for both first time and advanced users• Simple and flexible instrument set up and usageStepOne SoftwareThe StepOne Software included with the StepOne Real Time PCR System runs on Windows XP Window Vista and Windows 7 operating systems and provides instrument control data collection and data analysis capabilities This latest version includes capabilities to collect melt curve data for High Resolution Melt HRM applications and the option to export in Real Time PCR Data Markup Language RDML for compatibility with MIQE guidelines Rapid and Simple Assay Set UpThis remarkably simple Real Time PCR system is designed with an LCD touchscreen and a user friendly interface that brings the power of genetic studies to researchers new to Real Time PCR Fig 1 The intuitive software and protocol wizards help guide new users through their Real Time PCR experiments The StepOne Real Time PCR System also includes a quick start setup so that you start a run immediately and enter plate information at a later time Sensitive LED Based 3 Color Fluorescence ReadingThe StepOne Real Time PCR System utilizes a long life LED based optical system that can record fluorescence from FAM SYBR Green VIC JOE and ROX dyes This cost effective 3 color 48 well system delivers precise quantitative Real Time PCR results and saves data from all filters in every run without depending on a computer or plate setup It can discriminate between 2 populations of 5 000 and 10 000 template copies of a TaqMan assay with 99 7 confidence Compatible with Many Genomic Analysis TechniquesPerform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOne Real Time PCR System and software The StepOne System supports many Real Time PCR applications including the following • SNP Genotyping• Gene Expression Analysis• MicroRNA Expression• Translocation Analysis• Gene Detection• Viral Load AnalysisThe included StepOne Software supports a variety of analysis methods including the following • Standard Curve absolute quantitation • Relative Standard Curve• Comparative Ct ΔΔCt relative quantitation • Genotyping and Presence Absence• Melt Curve Analysis• High Resolution Melting additional software required Versatile Instrument Configuration OptionsThe ultra compact footprint of a StepOne System can be installed in multiple distinct configurations providing unmatched flexibility and convenience that can allow a fit in any laboratory Fig 2 The StepOne Real Time PCR System can be used via a PC with a PC connected to a Local Area Network LAN or as a stand alone instrument PC free Each StepOne Real Time PCR System is factory calibrated for optical and thermal accuracy so simply remarkable Real Time PCR results can be obtained right out of the box Real Time Data Monitoring Dissemination and StorageThe system measures amplification as it occurs allowing you to monitor the progress of your experiment cycle by cycle either on the machine or remotely Your data is stored on the instrument itself and can be viewed and stored automatically via remote access or transferred via email or a USB flash drive Data can also be conveniently exported as PowerPoint Excel and graphical image files Expanded Gene Expression CapabilitiesThe advanced software provided with the StepOne Real Time PCR System now includes the powerful Gene Expression Study Package This software package allows for greater flexibility and accuracy in your gene expression assays through • Analysis of an unlimited number of plates in one study• Sorting of data by biological or technical replicate group• Use of multiple endogenous controls• Assay efficiency correctionHigh Resolution Melting CapabilityNow with High Resolution Melt Software v3 0 you can perform post PCR sequence variation analysis with the StepOne Real Time PCR System This separately purchased software simplifies set up by maintaining assay specific settings and accepts pasted plate layout information directly from Excel HRM Software v3 0 also has an improved clustering algorithm for increased sensitivity and accuracy and the ability to conduct separate analyses of multiple assays run on a single plate For Research Use Only Not for use in diagnostics procedures
    Catalog Number:
    4376357
    Price:
    None
    Applications:
    Genotyping & Genomic Profiling|PCR & Real-Time PCR|Real Time PCR (qPCR)|Real Time PCR-Based Gene Expression Profiling|Real-Time PCR Instruments, Software & Calibration|Gene Expression Analysis & Genotyping|Genotyping Instruments, Software & Calibration|High Resolution Melting (HRM) Analysis
    Category:
    Instruments and Equipment
    Buy from Supplier


    Structured Review

    Thermo Fisher stepone real time polymerase chain reaction system
    The StepOne Real Time PCR System is a 48 well low throughput Real Time PCR instrument perfect for both first time and experienced users The StepOne Real Time PCR System can be setup in a variety of configurations and comes ready to use out of the box with intuitive data analysis and instrument control software Utilizing robust LED based 3 color optical recording the StepOne Real Time PCR System is designed to deliver precise quantitative Real Time PCR results for a variety of genomic research applications Features of the The StepOne Real Time PCR System include • Advanced software and instrumentation for performing a wide array of genomic assays • Sensitive 3 color optical LED recording system• Intuitive and robust software perfect for both first time and advanced users• Simple and flexible instrument set up and usageStepOne SoftwareThe StepOne Software included with the StepOne Real Time PCR System runs on Windows XP Window Vista and Windows 7 operating systems and provides instrument control data collection and data analysis capabilities This latest version includes capabilities to collect melt curve data for High Resolution Melt HRM applications and the option to export in Real Time PCR Data Markup Language RDML for compatibility with MIQE guidelines Rapid and Simple Assay Set UpThis remarkably simple Real Time PCR system is designed with an LCD touchscreen and a user friendly interface that brings the power of genetic studies to researchers new to Real Time PCR Fig 1 The intuitive software and protocol wizards help guide new users through their Real Time PCR experiments The StepOne Real Time PCR System also includes a quick start setup so that you start a run immediately and enter plate information at a later time Sensitive LED Based 3 Color Fluorescence ReadingThe StepOne Real Time PCR System utilizes a long life LED based optical system that can record fluorescence from FAM SYBR Green VIC JOE and ROX dyes This cost effective 3 color 48 well system delivers precise quantitative Real Time PCR results and saves data from all filters in every run without depending on a computer or plate setup It can discriminate between 2 populations of 5 000 and 10 000 template copies of a TaqMan assay with 99 7 confidence Compatible with Many Genomic Analysis TechniquesPerform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOne Real Time PCR System and software The StepOne System supports many Real Time PCR applications including the following • SNP Genotyping• Gene Expression Analysis• MicroRNA Expression• Translocation Analysis• Gene Detection• Viral Load AnalysisThe included StepOne Software supports a variety of analysis methods including the following • Standard Curve absolute quantitation • Relative Standard Curve• Comparative Ct ΔΔCt relative quantitation • Genotyping and Presence Absence• Melt Curve Analysis• High Resolution Melting additional software required Versatile Instrument Configuration OptionsThe ultra compact footprint of a StepOne System can be installed in multiple distinct configurations providing unmatched flexibility and convenience that can allow a fit in any laboratory Fig 2 The StepOne Real Time PCR System can be used via a PC with a PC connected to a Local Area Network LAN or as a stand alone instrument PC free Each StepOne Real Time PCR System is factory calibrated for optical and thermal accuracy so simply remarkable Real Time PCR results can be obtained right out of the box Real Time Data Monitoring Dissemination and StorageThe system measures amplification as it occurs allowing you to monitor the progress of your experiment cycle by cycle either on the machine or remotely Your data is stored on the instrument itself and can be viewed and stored automatically via remote access or transferred via email or a USB flash drive Data can also be conveniently exported as PowerPoint Excel and graphical image files Expanded Gene Expression CapabilitiesThe advanced software provided with the StepOne Real Time PCR System now includes the powerful Gene Expression Study Package This software package allows for greater flexibility and accuracy in your gene expression assays through • Analysis of an unlimited number of plates in one study• Sorting of data by biological or technical replicate group• Use of multiple endogenous controls• Assay efficiency correctionHigh Resolution Melting CapabilityNow with High Resolution Melt Software v3 0 you can perform post PCR sequence variation analysis with the StepOne Real Time PCR System This separately purchased software simplifies set up by maintaining assay specific settings and accepts pasted plate layout information directly from Excel HRM Software v3 0 also has an improved clustering algorithm for increased sensitivity and accuracy and the ability to conduct separate analyses of multiple assays run on a single plate For Research Use Only Not for use in diagnostics procedures
    https://www.bioz.com/result/stepone real time polymerase chain reaction system/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    stepone real time polymerase chain reaction system - by Bioz Stars, 2021-01
    99/100 stars

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    fast sybr green master mix
    real-time polymerase chain reaction

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Epigenetic regulation of CpG promoter methylation in invasive prostate cancer cells
    Article Snippet: .. Quantitative real time polymerase chain reaction (qRT-PCR) analysis was performed using a StepOne Real-time PCR machine (Applied Biosystems, Foster City, CA) with TaqMan Gene Expression Assay reagents and probes (Applied Biosystems). ..

    Article Title: Postnatal and non-invasive prenatal detection of β-thalassemia mutations based on Taqman genotyping assays
    Article Snippet: .. The reactions were carried out on a StepOne™ Real-Time PCR System (Applied Biosystems® —Thermo Fisher Scientific), by using the StepOne Software (Applied Biosystems® —Thermo Fisher Scientific). .. After a first 2-minute cycle at 50°C and a second 10-minute cycle at 95°C, the amplification cycles were different for each specific genotyping assay: 50 amplification cycles with denaturation at 95°C for 15 seconds, and annealing and elongation at 62°C for 1 minute for β0 39, β+ IVSI-110 and β+ IVSI-6 assays; 60 amplification cycles with denaturation at 95°C for 15 seconds, and annealing and elongation at 60°C for 1 minute for β0 IVSI-1.

    Article Title: Dicer Ablation Impairs Prostate Stem Cell Activity and Causes Prostate Atrophy
    Article Snippet: .. Quantitative RT-PCR was performed using the Taqman Power Sybrgreen master mix (Applied Biosystems) on a StepOne plus Real-Time PCR system (Applied Biosystems). .. Dissociated murine prostate cells were suspended in DMEM/10% FBS and stained with antibodies for 15 minutes at 4°C.

    Article Title: A dosage-dependent pleiotropic role of Dicer in prostate cancer growth and metastasis
    Article Snippet: .. Quantitative RT-PCR was performed using the Taqman microRNA assay (Applied Biosystems, Foster City, CA) and SYBR GreenER qPCR mix (BioRad, Hercules, CA) on a StepOne plus Real-Time PCR system (Applied Biosystems, Foster City, CA). ..

    Article Title: The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis
    Article Snippet: .. The real-time RT-qPCR was carried out with an SYBR Green PCR Master Mix (Enzynomics, Inc., Daejeon, Korea) and performed at an initial denaturation step at 95 °C for 10 min, followed by 40 cycles at 95 °C for 15 s, and finally 60 °C for 60 s in the Step-One™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). miR-10a and miR-126 3p were measured by using a hsa-mir-10a Real-Time RT-PCR detection kit (CPK1014) and a hsa-mir-126 3p Real-Time RT-PCR detection kit (CPK1083) (Cohesion Biosciences., London, UK). .. NO production in the culture supernatant was spectrophotometrically evaluated by measuring nitrite content, an oxidative product of NO. Nitrite levels was determined with the Griess Reagent solution (Promega, Madison, WI, USA) and proceeded according to the description of the manufacturer.

    Article Title: Role of PU.1 in MHC Class II Expression via CIITA Transcription in Plasmacytoid Dendritic Cells
    Article Snippet: .. The mRNA levels of PU.1, HLA-DRα and mouse MHC class II, CIITA, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were quantified by using a Step-One Real-Time PCR system (Applied Biosystems) with TaqMan Gene Expression Assays (Applied Biosystems: no. Hs02786711_m1 for human PU.1, Mm01270606_m1 for mouse PU.1, Hs00219575_m1 for HLA-DRα, Mm00772352_m1 for I-Eα, Hs00172106_m1 for human CIITA, Mm00482919_m1 for mouse CIITA, Mm01342720_m1 for mouse CIITA mRNA driven from promoter III (pIII-CIITA), human GAPDH no. 4326317E, and mouse GAPDH no. 4352339E) and TaqMan Universal Master Mix (Applied Biosystems). .. For the measurement of human pIII-CIITA, the following primers and probe were originally constructed using the customized service of Applied Biosystems: forward primer, 5’-GCTGGGATTCCTACACAATGC-3’; reverse primer, 5’-TCTCCAGCCAGGTCCATCTG-3’; and probe, 5’-FAM-CCCAAGGCAGCTCA-MGB-3’.

    Article Title: Regulation of PTEN by CK2 and Notch1 in primary T-cell acute lymphoblastic leukemia: rationale for combined use of CK2- and ?-secretase inhibitors
    Article Snippet: .. The quantitative assessment of HES1 , MYC and PTEN transcripts was made by Q-PCR on a StepOne Real-Time PCR System (Applied Biosystems). .. PCR products were cloned into the pGEM-T Easy vector (Promega) and standard curves were obtained by serial dilutions of uncut plasmid.

    Article Title: Expression of Glycogen synthase kinase 3-? (GSK3-?) gene in azoospermic men
    Article Snippet: .. Subsequent real time PCR was done with Power Syber Green real time master mix and Stepone real time PCR (Applied biosystems, Carlsbad, USA). ..

    Quantitative RT-PCR:

    Article Title: Epigenetic regulation of CpG promoter methylation in invasive prostate cancer cells
    Article Snippet: .. Quantitative real time polymerase chain reaction (qRT-PCR) analysis was performed using a StepOne Real-time PCR machine (Applied Biosystems, Foster City, CA) with TaqMan Gene Expression Assay reagents and probes (Applied Biosystems). ..

    Article Title: Dicer Ablation Impairs Prostate Stem Cell Activity and Causes Prostate Atrophy
    Article Snippet: .. Quantitative RT-PCR was performed using the Taqman Power Sybrgreen master mix (Applied Biosystems) on a StepOne plus Real-Time PCR system (Applied Biosystems). .. Dissociated murine prostate cells were suspended in DMEM/10% FBS and stained with antibodies for 15 minutes at 4°C.

    Article Title: A dosage-dependent pleiotropic role of Dicer in prostate cancer growth and metastasis
    Article Snippet: .. Quantitative RT-PCR was performed using the Taqman microRNA assay (Applied Biosystems, Foster City, CA) and SYBR GreenER qPCR mix (BioRad, Hercules, CA) on a StepOne plus Real-Time PCR system (Applied Biosystems, Foster City, CA). ..

    Article Title: The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis
    Article Snippet: .. The real-time RT-qPCR was carried out with an SYBR Green PCR Master Mix (Enzynomics, Inc., Daejeon, Korea) and performed at an initial denaturation step at 95 °C for 10 min, followed by 40 cycles at 95 °C for 15 s, and finally 60 °C for 60 s in the Step-One™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). miR-10a and miR-126 3p were measured by using a hsa-mir-10a Real-Time RT-PCR detection kit (CPK1014) and a hsa-mir-126 3p Real-Time RT-PCR detection kit (CPK1083) (Cohesion Biosciences., London, UK). .. NO production in the culture supernatant was spectrophotometrically evaluated by measuring nitrite content, an oxidative product of NO. Nitrite levels was determined with the Griess Reagent solution (Promega, Madison, WI, USA) and proceeded according to the description of the manufacturer.

    TaqMan microRNA Assay:

    Article Title: A dosage-dependent pleiotropic role of Dicer in prostate cancer growth and metastasis
    Article Snippet: .. Quantitative RT-PCR was performed using the Taqman microRNA assay (Applied Biosystems, Foster City, CA) and SYBR GreenER qPCR mix (BioRad, Hercules, CA) on a StepOne plus Real-Time PCR system (Applied Biosystems, Foster City, CA). ..

    SYBR Green Assay:

    Article Title: The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis
    Article Snippet: .. The real-time RT-qPCR was carried out with an SYBR Green PCR Master Mix (Enzynomics, Inc., Daejeon, Korea) and performed at an initial denaturation step at 95 °C for 10 min, followed by 40 cycles at 95 °C for 15 s, and finally 60 °C for 60 s in the Step-One™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). miR-10a and miR-126 3p were measured by using a hsa-mir-10a Real-Time RT-PCR detection kit (CPK1014) and a hsa-mir-126 3p Real-Time RT-PCR detection kit (CPK1083) (Cohesion Biosciences., London, UK). .. NO production in the culture supernatant was spectrophotometrically evaluated by measuring nitrite content, an oxidative product of NO. Nitrite levels was determined with the Griess Reagent solution (Promega, Madison, WI, USA) and proceeded according to the description of the manufacturer.

    Expressing:

    Article Title: Epigenetic regulation of CpG promoter methylation in invasive prostate cancer cells
    Article Snippet: .. Quantitative real time polymerase chain reaction (qRT-PCR) analysis was performed using a StepOne Real-time PCR machine (Applied Biosystems, Foster City, CA) with TaqMan Gene Expression Assay reagents and probes (Applied Biosystems). ..

    Article Title: Role of PU.1 in MHC Class II Expression via CIITA Transcription in Plasmacytoid Dendritic Cells
    Article Snippet: .. The mRNA levels of PU.1, HLA-DRα and mouse MHC class II, CIITA, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were quantified by using a Step-One Real-Time PCR system (Applied Biosystems) with TaqMan Gene Expression Assays (Applied Biosystems: no. Hs02786711_m1 for human PU.1, Mm01270606_m1 for mouse PU.1, Hs00219575_m1 for HLA-DRα, Mm00772352_m1 for I-Eα, Hs00172106_m1 for human CIITA, Mm00482919_m1 for mouse CIITA, Mm01342720_m1 for mouse CIITA mRNA driven from promoter III (pIII-CIITA), human GAPDH no. 4326317E, and mouse GAPDH no. 4352339E) and TaqMan Universal Master Mix (Applied Biosystems). .. For the measurement of human pIII-CIITA, the following primers and probe were originally constructed using the customized service of Applied Biosystems: forward primer, 5’-GCTGGGATTCCTACACAATGC-3’; reverse primer, 5’-TCTCCAGCCAGGTCCATCTG-3’; and probe, 5’-FAM-CCCAAGGCAGCTCA-MGB-3’.

    Polymerase Chain Reaction:

    Article Title: The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis
    Article Snippet: .. The real-time RT-qPCR was carried out with an SYBR Green PCR Master Mix (Enzynomics, Inc., Daejeon, Korea) and performed at an initial denaturation step at 95 °C for 10 min, followed by 40 cycles at 95 °C for 15 s, and finally 60 °C for 60 s in the Step-One™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). miR-10a and miR-126 3p were measured by using a hsa-mir-10a Real-Time RT-PCR detection kit (CPK1014) and a hsa-mir-126 3p Real-Time RT-PCR detection kit (CPK1083) (Cohesion Biosciences., London, UK). .. NO production in the culture supernatant was spectrophotometrically evaluated by measuring nitrite content, an oxidative product of NO. Nitrite levels was determined with the Griess Reagent solution (Promega, Madison, WI, USA) and proceeded according to the description of the manufacturer.

    Software:

    Article Title: Postnatal and non-invasive prenatal detection of β-thalassemia mutations based on Taqman genotyping assays
    Article Snippet: .. The reactions were carried out on a StepOne™ Real-Time PCR System (Applied Biosystems® —Thermo Fisher Scientific), by using the StepOne Software (Applied Biosystems® —Thermo Fisher Scientific). .. After a first 2-minute cycle at 50°C and a second 10-minute cycle at 95°C, the amplification cycles were different for each specific genotyping assay: 50 amplification cycles with denaturation at 95°C for 15 seconds, and annealing and elongation at 62°C for 1 minute for β0 39, β+ IVSI-110 and β+ IVSI-6 assays; 60 amplification cycles with denaturation at 95°C for 15 seconds, and annealing and elongation at 60°C for 1 minute for β0 IVSI-1.

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  • 99
    Thermo Fisher real time pcr system
    ENO1 protein and mRNA levels in a panel of ovarian cancer cells. (A) Western blot analysis was performed using protein extracts (30-50 µg) of cisplatin-sensitive (A2780, OV-90, and OVCAR3) and cisplatin-resistant (A280CP20, A2780CIS, OV-90CIS, and OVCAR3CIS) ovarian cancer cells. (B) Densitometry analysis of band intensities, shown in (A). Fold changes in protein levels were calculated relative to the cisplatin sensitive pair. Averages ± SEM are shown for three independent experiments (C) ENO1 mRNA expression levels were assessed by <t>qPCR.</t> β-actin was used as a <t>PCR</t> internal control. Fold changes in mRNA levels were calculated relative to the cisplatin sensitive pair. Averages ± SEM are shown for three independent experiments. *P
    Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1761 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr system/product/Thermo Fisher
    Average 99 stars, based on 1761 article reviews
    Price from $9.99 to $1999.99
    real time pcr system - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher human a2ar
    <t>A2AR</t> −/− primary chondrocytes have altered mitochondrial ultrastructure under TEM. Primary WT and A2AR −/− chondrocytes were isolated from neonatal mice and (A) submitted to TEM to evaluate mitochondrial structure, at least 30 mitochondrion per condition were analyzed (scale = 0.5 μm). (B) Cristae widths were significantly greater in A2AR −/− chondrocytes’ mitochondria while there were no significant changes in (C) number of cristae per mitochondria, (D) cristae junction (CJ) per mitochondria or (E) in the ratio of the number of CJ to the number of cristae ( P
    Human A2ar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human a2ar/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human a2ar - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

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    ENO1 protein and mRNA levels in a panel of ovarian cancer cells. (A) Western blot analysis was performed using protein extracts (30-50 µg) of cisplatin-sensitive (A2780, OV-90, and OVCAR3) and cisplatin-resistant (A280CP20, A2780CIS, OV-90CIS, and OVCAR3CIS) ovarian cancer cells. (B) Densitometry analysis of band intensities, shown in (A). Fold changes in protein levels were calculated relative to the cisplatin sensitive pair. Averages ± SEM are shown for three independent experiments (C) ENO1 mRNA expression levels were assessed by qPCR. β-actin was used as a PCR internal control. Fold changes in mRNA levels were calculated relative to the cisplatin sensitive pair. Averages ± SEM are shown for three independent experiments. *P

    Journal: American Journal of Translational Research

    Article Title: Reduced expression of enolase-1 correlates with high intracellular glucose levels and increased senescence in cisplatin-resistant ovarian cancer cells

    doi:

    Figure Lengend Snippet: ENO1 protein and mRNA levels in a panel of ovarian cancer cells. (A) Western blot analysis was performed using protein extracts (30-50 µg) of cisplatin-sensitive (A2780, OV-90, and OVCAR3) and cisplatin-resistant (A280CP20, A2780CIS, OV-90CIS, and OVCAR3CIS) ovarian cancer cells. (B) Densitometry analysis of band intensities, shown in (A). Fold changes in protein levels were calculated relative to the cisplatin sensitive pair. Averages ± SEM are shown for three independent experiments (C) ENO1 mRNA expression levels were assessed by qPCR. β-actin was used as a PCR internal control. Fold changes in mRNA levels were calculated relative to the cisplatin sensitive pair. Averages ± SEM are shown for three independent experiments. *P

    Article Snippet: SYBR-I-based Real-Time PCR (qPCR) was performed in a StepOne plus Real-Time PCR System (Thermo Scientific).

    Techniques: Western Blot, Expressing, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

    Scatter dot plot for each qRT-PCR validation sample. A) Comparison of the expression levels of JUN for U937 cells exposed to nsEP. B) Comparison of the expression levels of JUN for Jurkat cells exposed to nsEP. C) Comparison of the expression levels of DUSP10 for U937 cells exposed to nsEP. D) Comparison of the expression levels of DUSP10 for Jurkat cells exposed to nsEP. E) Comparison of the expression levels of HPRT1 for U937 cells exposed to nsEP. F) Comparison of the expression levels of HPRT1 for Jurkat cells exposed to nsEP. Mean and standard deviation are plotted as the green and black lines respectively.

    Journal: PLoS ONE

    Article Title: Evaluation of the Genetic Response of U937 and Jurkat Cells to 10-Nanosecond Electrical Pulses (nsEP)

    doi: 10.1371/journal.pone.0154555

    Figure Lengend Snippet: Scatter dot plot for each qRT-PCR validation sample. A) Comparison of the expression levels of JUN for U937 cells exposed to nsEP. B) Comparison of the expression levels of JUN for Jurkat cells exposed to nsEP. C) Comparison of the expression levels of DUSP10 for U937 cells exposed to nsEP. D) Comparison of the expression levels of DUSP10 for Jurkat cells exposed to nsEP. E) Comparison of the expression levels of HPRT1 for U937 cells exposed to nsEP. F) Comparison of the expression levels of HPRT1 for Jurkat cells exposed to nsEP. Mean and standard deviation are plotted as the green and black lines respectively.

    Article Snippet: Quantitative Real Time Polymerase Chain Reaction Each gene selected for validation was validated by quantitative real time polymerase chain reaction (qRT-PCR) using the Applied Biosystems StepOne™ Plus PCR system from ThermoFisher Scientific (Carlsbad, CA).

    Techniques: Quantitative RT-PCR, Expressing, Standard Deviation

    Comparison of exosomal miR-223-3p levels between DCIS patients, upstage IDC patients and IDC patients. Exosomal miR-223-3p levels of DCIS patients (Stage 0), upstaged IDC patients (Stage I) and IDC patients (Stage I) were measured by reverse transcription-quantitative polymerase chain reaction. DCIS patients who were initially diagnosed by a needle biopsy prior to surgery were re-diagnosed following the operation using the completely excised specimen. Patients upstaged to IDC from DCIS on the final pathological report were described as upstage IDC patients. DCIS, ductal carcinoma in situ ; IDC, invasive ductal carcinoma; miR, microRNA.

    Journal: Oncology Letters

    Article Title: Exosome-encapsulated microRNA-223-3p as a minimally invasive biomarker for the early detection of invasive breast cancer

    doi: 10.3892/ol.2018.8457

    Figure Lengend Snippet: Comparison of exosomal miR-223-3p levels between DCIS patients, upstage IDC patients and IDC patients. Exosomal miR-223-3p levels of DCIS patients (Stage 0), upstaged IDC patients (Stage I) and IDC patients (Stage I) were measured by reverse transcription-quantitative polymerase chain reaction. DCIS patients who were initially diagnosed by a needle biopsy prior to surgery were re-diagnosed following the operation using the completely excised specimen. Patients upstaged to IDC from DCIS on the final pathological report were described as upstage IDC patients. DCIS, ductal carcinoma in situ ; IDC, invasive ductal carcinoma; miR, microRNA.

    Article Snippet: Expressions of miR-223-3p of pCMV-pre-miR-223-3p or wpCMV-miR-neg-transfected MCF7 cells were measured by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) system (StepOne; Thermo Fisher Scientific, Inc.) and Digital PCR system (Quant Studio 3D Digital PCR System; Thermo Fisher Scientific, Inc.).

    Techniques: Real-time Polymerase Chain Reaction, In Situ

    A2AR −/− primary chondrocytes have altered mitochondrial ultrastructure under TEM. Primary WT and A2AR −/− chondrocytes were isolated from neonatal mice and (A) submitted to TEM to evaluate mitochondrial structure, at least 30 mitochondrion per condition were analyzed (scale = 0.5 μm). (B) Cristae widths were significantly greater in A2AR −/− chondrocytes’ mitochondria while there were no significant changes in (C) number of cristae per mitochondria, (D) cristae junction (CJ) per mitochondria or (E) in the ratio of the number of CJ to the number of cristae ( P

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: Adenosine A2A receptor (A2AR) stimulation enhances mitochondrial metabolism and mitigates reactive oxygen species-mediated mitochondrial injury

    doi: 10.1096/fj.201902459R

    Figure Lengend Snippet: A2AR −/− primary chondrocytes have altered mitochondrial ultrastructure under TEM. Primary WT and A2AR −/− chondrocytes were isolated from neonatal mice and (A) submitted to TEM to evaluate mitochondrial structure, at least 30 mitochondrion per condition were analyzed (scale = 0.5 μm). (B) Cristae widths were significantly greater in A2AR −/− chondrocytes’ mitochondria while there were no significant changes in (C) number of cristae per mitochondria, (D) cristae junction (CJ) per mitochondria or (E) in the ratio of the number of CJ to the number of cristae ( P

    Article Snippet: After RNA reverse transcription to cDNA, real time PCR reactions were performed for a relative quantification of human A2AR (normalized to human GAPDH) StepOne Real-Time PCR Sysyem (Thermo Fisher Scientific) with Brilliant SYBR Green Kit QPCR Master Mix, according to the manufacturer’s protocol.

    Techniques: Transmission Electron Microscopy, Isolation, Mouse Assay

    IL-1β increases mRNA levels and total protein expression of the A2AR in both cytoplasm and plasma membrane which potentiates the effects of receptor ligation in the setting of inflammation. T/C-28a2 cells were incubated with medium (IL1B−) or IL-1β (5ng/mL overnight, IL1B+). Whole cell lysates were prepared to isolate protein from the cytosol and plasma membrane compartments, where IL-1β exposure increased protein levels. mRNA levels for A2AR were also upregulated (normalized to GAPDH)

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: Adenosine A2A receptor (A2AR) stimulation enhances mitochondrial metabolism and mitigates reactive oxygen species-mediated mitochondrial injury

    doi: 10.1096/fj.201902459R

    Figure Lengend Snippet: IL-1β increases mRNA levels and total protein expression of the A2AR in both cytoplasm and plasma membrane which potentiates the effects of receptor ligation in the setting of inflammation. T/C-28a2 cells were incubated with medium (IL1B−) or IL-1β (5ng/mL overnight, IL1B+). Whole cell lysates were prepared to isolate protein from the cytosol and plasma membrane compartments, where IL-1β exposure increased protein levels. mRNA levels for A2AR were also upregulated (normalized to GAPDH)

    Article Snippet: After RNA reverse transcription to cDNA, real time PCR reactions were performed for a relative quantification of human A2AR (normalized to human GAPDH) StepOne Real-Time PCR Sysyem (Thermo Fisher Scientific) with Brilliant SYBR Green Kit QPCR Master Mix, according to the manufacturer’s protocol.

    Techniques: Expressing, Ligation, Incubation