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OriginLab statistical ana lysis two sample t tests
MET and epidermal growth factor receptor (EGFR) densities in the cell membrane of HeLa and BT-20 cells. ( a ) Concept of super-resolution microscopy by Exchange-PAINT of MET and EGFR (top). A super-resolved image is obtained from transient binding events of short, fluorescently labeled DNA oligonucleotides to DNA-labeled antibodies. The image (bottom) shows MET visualized by widefield microscopy versus DNA-PAINT (scale bar 5 µm). ( b ) Exchange-PAINT images of MET (cyan) and EGFR (magenta) immunostained with secondary antibodies carrying P5 and P1 DNA docking strands and labeled with complementary DNA imager strands labeled with ATTO 655. Total internal reflection fluorescence (TIRF) images of the plasma membrane of HeLa and BT-20 cells were recorded either in the unstimulated state, after hepatocycte growth factor (HGF) stimulation, or after activation with epidermal growth factor (EGF) (scale bar 5 µm, insets 5 µm × 5 µm). Receptor cluster densities of MET (cyan) and EGFR (magenta) in ( c ) HeLa and ( d ) BT-20 cells were determined from DNA-PAINT images ( n  = 6–7 cells/condition from at least three independent experiments) and plotted in the histogram (left). (Note that receptor clusters refer to both monomers and dimers.) Error bars represent standard deviations. Results of two-sample t-tests for comparison of activated samples with the respective unstimulated sample are depicted as arrows ( p >  0.05 no significant difference between populations (n.s.),  p
Statistical Ana Lysis Two Sample T Tests, supplied by OriginLab, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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1) Product Images from "Single-Molecule Super-Resolution Microscopy Reveals Heteromeric Complexes of MET and EGFR upon Ligand Activation"

Article Title: Single-Molecule Super-Resolution Microscopy Reveals Heteromeric Complexes of MET and EGFR upon Ligand Activation

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms21082803

MET and epidermal growth factor receptor (EGFR) densities in the cell membrane of HeLa and BT-20 cells. ( a ) Concept of super-resolution microscopy by Exchange-PAINT of MET and EGFR (top). A super-resolved image is obtained from transient binding events of short, fluorescently labeled DNA oligonucleotides to DNA-labeled antibodies. The image (bottom) shows MET visualized by widefield microscopy versus DNA-PAINT (scale bar 5 µm). ( b ) Exchange-PAINT images of MET (cyan) and EGFR (magenta) immunostained with secondary antibodies carrying P5 and P1 DNA docking strands and labeled with complementary DNA imager strands labeled with ATTO 655. Total internal reflection fluorescence (TIRF) images of the plasma membrane of HeLa and BT-20 cells were recorded either in the unstimulated state, after hepatocycte growth factor (HGF) stimulation, or after activation with epidermal growth factor (EGF) (scale bar 5 µm, insets 5 µm × 5 µm). Receptor cluster densities of MET (cyan) and EGFR (magenta) in ( c ) HeLa and ( d ) BT-20 cells were determined from DNA-PAINT images ( n  = 6–7 cells/condition from at least three independent experiments) and plotted in the histogram (left). (Note that receptor clusters refer to both monomers and dimers.) Error bars represent standard deviations. Results of two-sample t-tests for comparison of activated samples with the respective unstimulated sample are depicted as arrows ( p >  0.05 no significant difference between populations (n.s.),  p
Figure Legend Snippet: MET and epidermal growth factor receptor (EGFR) densities in the cell membrane of HeLa and BT-20 cells. ( a ) Concept of super-resolution microscopy by Exchange-PAINT of MET and EGFR (top). A super-resolved image is obtained from transient binding events of short, fluorescently labeled DNA oligonucleotides to DNA-labeled antibodies. The image (bottom) shows MET visualized by widefield microscopy versus DNA-PAINT (scale bar 5 µm). ( b ) Exchange-PAINT images of MET (cyan) and EGFR (magenta) immunostained with secondary antibodies carrying P5 and P1 DNA docking strands and labeled with complementary DNA imager strands labeled with ATTO 655. Total internal reflection fluorescence (TIRF) images of the plasma membrane of HeLa and BT-20 cells were recorded either in the unstimulated state, after hepatocycte growth factor (HGF) stimulation, or after activation with epidermal growth factor (EGF) (scale bar 5 µm, insets 5 µm × 5 µm). Receptor cluster densities of MET (cyan) and EGFR (magenta) in ( c ) HeLa and ( d ) BT-20 cells were determined from DNA-PAINT images ( n = 6–7 cells/condition from at least three independent experiments) and plotted in the histogram (left). (Note that receptor clusters refer to both monomers and dimers.) Error bars represent standard deviations. Results of two-sample t-tests for comparison of activated samples with the respective unstimulated sample are depicted as arrows ( p > 0.05 no significant difference between populations (n.s.), p

Techniques Used: Microscopy, Binding Assay, Labeling, Fluorescence, Activation Assay

Diffusion dynamics of MET and EGFR in HeLa and BT-20 cells after stimulation with HGF and EGF studied by SPT indicate receptor cross-interactions. ( a , d ) MET was tracked by adding Fab-ATTO 647N. EGFR was tracked by adding EGFR-SOMAmer reagent modified with a P1-docking strand and P1-Cy3B for PAINT imaging. The Fab fragment and the SOMAmer reagent bind to the respective receptor but do not activate them. For observations of direct activation or possible cross-interaction, unlabeled HGF (light blue) or EGF (purple) were added to the samples. Diffusion coefficients of MET and EGFR in resting and activated cells (each  n  = 50 from at least three independent experiments) were determined in ( b , e ) HeLa and ( c , f ) BT-20 cells. All diffusion coefficients were normalized against reference measurements of ligand-untreated cells for all types of treatment. The box plots of diffusion coefficients display the 5th percentile, 25th percentile, median (line), mean (square), 75th percentile, and 95th percentile. Results of two-sample t-tests for comparison of ligand-treated cells with the reference are depicted above the box plots ( p >  0.05 no significant difference betw een populations (n.s.),  p
Figure Legend Snippet: Diffusion dynamics of MET and EGFR in HeLa and BT-20 cells after stimulation with HGF and EGF studied by SPT indicate receptor cross-interactions. ( a , d ) MET was tracked by adding Fab-ATTO 647N. EGFR was tracked by adding EGFR-SOMAmer reagent modified with a P1-docking strand and P1-Cy3B for PAINT imaging. The Fab fragment and the SOMAmer reagent bind to the respective receptor but do not activate them. For observations of direct activation or possible cross-interaction, unlabeled HGF (light blue) or EGF (purple) were added to the samples. Diffusion coefficients of MET and EGFR in resting and activated cells (each n = 50 from at least three independent experiments) were determined in ( b , e ) HeLa and ( c , f ) BT-20 cells. All diffusion coefficients were normalized against reference measurements of ligand-untreated cells for all types of treatment. The box plots of diffusion coefficients display the 5th percentile, 25th percentile, median (line), mean (square), 75th percentile, and 95th percentile. Results of two-sample t-tests for comparison of ligand-treated cells with the reference are depicted above the box plots ( p > 0.05 no significant difference betw een populations (n.s.), p

Techniques Used: Diffusion-based Assay, Single-particle Tracking, Modification, Imaging, Activation Assay

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    OriginLab statistical ana lysis two sample t tests
    MET and epidermal growth factor receptor (EGFR) densities in the cell membrane of HeLa and BT-20 cells. ( a ) Concept of super-resolution microscopy by Exchange-PAINT of MET and EGFR (top). A super-resolved image is obtained from transient binding events of short, fluorescently labeled DNA oligonucleotides to DNA-labeled antibodies. The image (bottom) shows MET visualized by widefield microscopy versus DNA-PAINT (scale bar 5 µm). ( b ) Exchange-PAINT images of MET (cyan) and EGFR (magenta) immunostained with secondary antibodies carrying P5 and P1 DNA docking strands and labeled with complementary DNA imager strands labeled with ATTO 655. Total internal reflection fluorescence (TIRF) images of the plasma membrane of HeLa and BT-20 cells were recorded either in the unstimulated state, after hepatocycte growth factor (HGF) stimulation, or after activation with epidermal growth factor (EGF) (scale bar 5 µm, insets 5 µm × 5 µm). Receptor cluster densities of MET (cyan) and EGFR (magenta) in ( c ) HeLa and ( d ) BT-20 cells were determined from DNA-PAINT images ( n  = 6–7 cells/condition from at least three independent experiments) and plotted in the histogram (left). (Note that receptor clusters refer to both monomers and dimers.) Error bars represent standard deviations. Results of two-sample t-tests for comparison of activated samples with the respective unstimulated sample are depicted as arrows ( p >  0.05 no significant difference between populations (n.s.),  p
    Statistical Ana Lysis Two Sample T Tests, supplied by OriginLab, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/statistical ana lysis two sample t tests/product/OriginLab
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    statistical ana lysis two sample t tests - by Bioz Stars, 2022-09
    99/100 stars
      Buy from Supplier

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    MET and epidermal growth factor receptor (EGFR) densities in the cell membrane of HeLa and BT-20 cells. ( a ) Concept of super-resolution microscopy by Exchange-PAINT of MET and EGFR (top). A super-resolved image is obtained from transient binding events of short, fluorescently labeled DNA oligonucleotides to DNA-labeled antibodies. The image (bottom) shows MET visualized by widefield microscopy versus DNA-PAINT (scale bar 5 µm). ( b ) Exchange-PAINT images of MET (cyan) and EGFR (magenta) immunostained with secondary antibodies carrying P5 and P1 DNA docking strands and labeled with complementary DNA imager strands labeled with ATTO 655. Total internal reflection fluorescence (TIRF) images of the plasma membrane of HeLa and BT-20 cells were recorded either in the unstimulated state, after hepatocycte growth factor (HGF) stimulation, or after activation with epidermal growth factor (EGF) (scale bar 5 µm, insets 5 µm × 5 µm). Receptor cluster densities of MET (cyan) and EGFR (magenta) in ( c ) HeLa and ( d ) BT-20 cells were determined from DNA-PAINT images ( n  = 6–7 cells/condition from at least three independent experiments) and plotted in the histogram (left). (Note that receptor clusters refer to both monomers and dimers.) Error bars represent standard deviations. Results of two-sample t-tests for comparison of activated samples with the respective unstimulated sample are depicted as arrows ( p >  0.05 no significant difference between populations (n.s.),  p

    Journal: International Journal of Molecular Sciences

    Article Title: Single-Molecule Super-Resolution Microscopy Reveals Heteromeric Complexes of MET and EGFR upon Ligand Activation

    doi: 10.3390/ijms21082803

    Figure Lengend Snippet: MET and epidermal growth factor receptor (EGFR) densities in the cell membrane of HeLa and BT-20 cells. ( a ) Concept of super-resolution microscopy by Exchange-PAINT of MET and EGFR (top). A super-resolved image is obtained from transient binding events of short, fluorescently labeled DNA oligonucleotides to DNA-labeled antibodies. The image (bottom) shows MET visualized by widefield microscopy versus DNA-PAINT (scale bar 5 µm). ( b ) Exchange-PAINT images of MET (cyan) and EGFR (magenta) immunostained with secondary antibodies carrying P5 and P1 DNA docking strands and labeled with complementary DNA imager strands labeled with ATTO 655. Total internal reflection fluorescence (TIRF) images of the plasma membrane of HeLa and BT-20 cells were recorded either in the unstimulated state, after hepatocycte growth factor (HGF) stimulation, or after activation with epidermal growth factor (EGF) (scale bar 5 µm, insets 5 µm × 5 µm). Receptor cluster densities of MET (cyan) and EGFR (magenta) in ( c ) HeLa and ( d ) BT-20 cells were determined from DNA-PAINT images ( n = 6–7 cells/condition from at least three independent experiments) and plotted in the histogram (left). (Note that receptor clusters refer to both monomers and dimers.) Error bars represent standard deviations. Results of two-sample t-tests for comparison of activated samples with the respective unstimulated sample are depicted as arrows ( p > 0.05 no significant difference between populations (n.s.), p

    Article Snippet: Statistical Ana lysis Two-sample t-tests were performed in OriginPro (version 2016G, Origin Lab Corporation, Northampton, MA, USA) to test the significance of differences between the chosen conditions for receptor densities determined from DNA-PAINT data ( c,d) and for normalized diffusion coefficients ( b,e).

    Techniques: Microscopy, Binding Assay, Labeling, Fluorescence, Activation Assay

    Diffusion dynamics of MET and EGFR in HeLa and BT-20 cells after stimulation with HGF and EGF studied by SPT indicate receptor cross-interactions. ( a , d ) MET was tracked by adding Fab-ATTO 647N. EGFR was tracked by adding EGFR-SOMAmer reagent modified with a P1-docking strand and P1-Cy3B for PAINT imaging. The Fab fragment and the SOMAmer reagent bind to the respective receptor but do not activate them. For observations of direct activation or possible cross-interaction, unlabeled HGF (light blue) or EGF (purple) were added to the samples. Diffusion coefficients of MET and EGFR in resting and activated cells (each  n  = 50 from at least three independent experiments) were determined in ( b , e ) HeLa and ( c , f ) BT-20 cells. All diffusion coefficients were normalized against reference measurements of ligand-untreated cells for all types of treatment. The box plots of diffusion coefficients display the 5th percentile, 25th percentile, median (line), mean (square), 75th percentile, and 95th percentile. Results of two-sample t-tests for comparison of ligand-treated cells with the reference are depicted above the box plots ( p >  0.05 no significant difference betw een populations (n.s.),  p

    Journal: International Journal of Molecular Sciences

    Article Title: Single-Molecule Super-Resolution Microscopy Reveals Heteromeric Complexes of MET and EGFR upon Ligand Activation

    doi: 10.3390/ijms21082803

    Figure Lengend Snippet: Diffusion dynamics of MET and EGFR in HeLa and BT-20 cells after stimulation with HGF and EGF studied by SPT indicate receptor cross-interactions. ( a , d ) MET was tracked by adding Fab-ATTO 647N. EGFR was tracked by adding EGFR-SOMAmer reagent modified with a P1-docking strand and P1-Cy3B for PAINT imaging. The Fab fragment and the SOMAmer reagent bind to the respective receptor but do not activate them. For observations of direct activation or possible cross-interaction, unlabeled HGF (light blue) or EGF (purple) were added to the samples. Diffusion coefficients of MET and EGFR in resting and activated cells (each n = 50 from at least three independent experiments) were determined in ( b , e ) HeLa and ( c , f ) BT-20 cells. All diffusion coefficients were normalized against reference measurements of ligand-untreated cells for all types of treatment. The box plots of diffusion coefficients display the 5th percentile, 25th percentile, median (line), mean (square), 75th percentile, and 95th percentile. Results of two-sample t-tests for comparison of ligand-treated cells with the reference are depicted above the box plots ( p > 0.05 no significant difference betw een populations (n.s.), p

    Article Snippet: Statistical Ana lysis Two-sample t-tests were performed in OriginPro (version 2016G, Origin Lab Corporation, Northampton, MA, USA) to test the significance of differences between the chosen conditions for receptor densities determined from DNA-PAINT data ( c,d) and for normalized diffusion coefficients ( b,e).

    Techniques: Diffusion-based Assay, Single-particle Tracking, Modification, Imaging, Activation Assay