stability enhanced non targeting dsrna control oligonucleotide  (Thermo Fisher)


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    Structured Review

    Thermo Fisher stability enhanced non targeting dsrna control oligonucleotide
    Stability Enhanced Non Targeting Dsrna Control Oligonucleotide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stability enhanced non targeting dsrna control oligonucleotide/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    stability enhanced non targeting dsrna control oligonucleotide - by Bioz Stars, 2020-07
    85/100 stars

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    Related Articles

    Transfection:

    Article Title: MicroRNA-486-3p Regulates γ-Globin Expression in Human Erythroid Cells by Directly Modulating BCL11A
    Article Snippet: .. Cells were seeded (1,25×105 cells/mL) in antibiotic-free media and transfected with 160 nM of a stability-enhanced miRNA-486-3p (Ambion), or with 160 nM of a stability enhanced non-targeting dsRNA control oligonucleotide (Ambion); for in vivo knockdown of miR-486-3p was used 160 nM of either anti miR-486-3p LNA-modified oligonucletide (Exiqon) and a LNA-modified non-targeting control miRNA (Exiqon). .. The LNA-modified and the stability-enhanced oligonucleotides were delivered by Lipofectamine 2000.

    Luciferase:

    Article Title: MicroRNA-486-3p Regulates γ-Globin Expression in Human Erythroid Cells by Directly Modulating BCL11A
    Article Snippet: .. For luciferase assay cells were co-transfected with 0.8 µg of pGL3-3′UTR plasmid, 0.1 µg of Renilla expressing vector and 40 pmol of either a stability enhanced non-targeting dsRNA control oligonucleotide (Ambion,) or a stability-enhanced miRNA-486-3p (Ambion), all combined with Lipofectamine2000 (Invitrogen). ..

    In Vivo:

    Article Title: MicroRNA-486-3p Regulates γ-Globin Expression in Human Erythroid Cells by Directly Modulating BCL11A
    Article Snippet: .. Cells were seeded (1,25×105 cells/mL) in antibiotic-free media and transfected with 160 nM of a stability-enhanced miRNA-486-3p (Ambion), or with 160 nM of a stability enhanced non-targeting dsRNA control oligonucleotide (Ambion); for in vivo knockdown of miR-486-3p was used 160 nM of either anti miR-486-3p LNA-modified oligonucletide (Exiqon) and a LNA-modified non-targeting control miRNA (Exiqon). .. The LNA-modified and the stability-enhanced oligonucleotides were delivered by Lipofectamine 2000.

    Plasmid Preparation:

    Article Title: MicroRNA-486-3p Regulates γ-Globin Expression in Human Erythroid Cells by Directly Modulating BCL11A
    Article Snippet: .. For luciferase assay cells were co-transfected with 0.8 µg of pGL3-3′UTR plasmid, 0.1 µg of Renilla expressing vector and 40 pmol of either a stability enhanced non-targeting dsRNA control oligonucleotide (Ambion,) or a stability-enhanced miRNA-486-3p (Ambion), all combined with Lipofectamine2000 (Invitrogen). ..

    Expressing:

    Article Title: MicroRNA-486-3p Regulates γ-Globin Expression in Human Erythroid Cells by Directly Modulating BCL11A
    Article Snippet: .. For luciferase assay cells were co-transfected with 0.8 µg of pGL3-3′UTR plasmid, 0.1 µg of Renilla expressing vector and 40 pmol of either a stability enhanced non-targeting dsRNA control oligonucleotide (Ambion,) or a stability-enhanced miRNA-486-3p (Ambion), all combined with Lipofectamine2000 (Invitrogen). ..

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    Thermo Fisher rna preparation dsrna substrates
    Dcl5 Cleavage of <t>dsRNA</t> Templates with and without Nucleotide Substitutions in IES-IES Junction Sequence (A) dsRNA templates used for processing assays are shown schematically. Blue, purple, and orange represent different IES sequences. Red line indicates IES-IES junction. IES-IES junction sequence is shown in black font. Nucleotide substitutions are shown in red. (B and C) 15% polyacrylamide-urea gel with small RNAs 5′-labeled with 32 P for the cleavage with MAC.IES templates (B) and single mutated MAC.IES templates (C). M is the small <t>RNA</t> ladder. (+) and (−) indicate reactions performed with or without the enzyme. Dashed boxes indicate the bands corresponding to the most abundant products after sRNA sequencing (see Figure S2 ). (D and E) Mapping of all 27 nt long sRNA reads to the MAC.IES templates (D) and to the single mutated MAC.IES templates (E) used for processing assays. Arrow indicates the sRNA peaks most affected upon changes in the template sequence.
    Rna Preparation Dsrna Substrates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna preparation dsrna substrates/product/Thermo Fisher
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    rna preparation dsrna substrates - by Bioz Stars, 2020-07
    92/100 stars
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    94
    Thermo Fisher t7 scribe standard rna ivt kit
    Dcl5 Cleavage of <t>dsRNA</t> Templates with and without Nucleotide Substitutions in IES-IES Junction Sequence (A) dsRNA templates used for processing assays are shown schematically. Blue, purple, and orange represent different IES sequences. Red line indicates IES-IES junction. IES-IES junction sequence is shown in black font. Nucleotide substitutions are shown in red. (B and C) 15% polyacrylamide-urea gel with small RNAs 5′-labeled with 32 P for the cleavage with MAC.IES templates (B) and single mutated MAC.IES templates (C). M is the small <t>RNA</t> ladder. (+) and (−) indicate reactions performed with or without the enzyme. Dashed boxes indicate the bands corresponding to the most abundant products after sRNA sequencing (see Figure S2 ). (D and E) Mapping of all 27 nt long sRNA reads to the MAC.IES templates (D) and to the single mutated MAC.IES templates (E) used for processing assays. Arrow indicates the sRNA peaks most affected upon changes in the template sequence.
    T7 Scribe Standard Rna Ivt Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t7 scribe standard rna ivt kit/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t7 scribe standard rna ivt kit - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    Dcl5 Cleavage of dsRNA Templates with and without Nucleotide Substitutions in IES-IES Junction Sequence (A) dsRNA templates used for processing assays are shown schematically. Blue, purple, and orange represent different IES sequences. Red line indicates IES-IES junction. IES-IES junction sequence is shown in black font. Nucleotide substitutions are shown in red. (B and C) 15% polyacrylamide-urea gel with small RNAs 5′-labeled with 32 P for the cleavage with MAC.IES templates (B) and single mutated MAC.IES templates (C). M is the small RNA ladder. (+) and (−) indicate reactions performed with or without the enzyme. Dashed boxes indicate the bands corresponding to the most abundant products after sRNA sequencing (see Figure S2 ). (D and E) Mapping of all 27 nt long sRNA reads to the MAC.IES templates (D) and to the single mutated MAC.IES templates (E) used for processing assays. Arrow indicates the sRNA peaks most affected upon changes in the template sequence.

    Journal: Cell

    Article Title: Dicer-like Enzymes with Sequence Cleavage Preferences

    doi: 10.1016/j.cell.2018.02.029

    Figure Lengend Snippet: Dcl5 Cleavage of dsRNA Templates with and without Nucleotide Substitutions in IES-IES Junction Sequence (A) dsRNA templates used for processing assays are shown schematically. Blue, purple, and orange represent different IES sequences. Red line indicates IES-IES junction. IES-IES junction sequence is shown in black font. Nucleotide substitutions are shown in red. (B and C) 15% polyacrylamide-urea gel with small RNAs 5′-labeled with 32 P for the cleavage with MAC.IES templates (B) and single mutated MAC.IES templates (C). M is the small RNA ladder. (+) and (−) indicate reactions performed with or without the enzyme. Dashed boxes indicate the bands corresponding to the most abundant products after sRNA sequencing (see Figure S2 ). (D and E) Mapping of all 27 nt long sRNA reads to the MAC.IES templates (D) and to the single mutated MAC.IES templates (E) used for processing assays. Arrow indicates the sRNA peaks most affected upon changes in the template sequence.

    Article Snippet: RNA preparation DsRNA substrates were produced by T7 in vitro transcription with the MEGAscript T7 transcription kit (Thermo Scientific).

    Techniques: Sequencing, Labeling

    In Vitro dsRNA Processing by Dcl4 (A) Expression profiles of four Dicer-like enzymes according to microarray data. y axis shows the expression level. x axis shows the developmental stages. (B) 15% polyacrylamide-urea gel with small RNAs 5′-labeled with 32 P. M is the small RNA ladder. No enzyme controls are marked as (−). “Beads only” is a no template RNA control for Dcl4. (C) Size distribution graphs of Dcl4 cleavage products based on sRNA sequencing. (D–F) 5′ nucleotide enrichment (D) and 3′ end nucleotide enrichment graph (E) for Dcl4 cleavage products showing relative abundance of the nucleotides. The sequence logos are shown for 25 nt sRNAs (F). A typical Dcl4 double-stranded cleavage product is shown schematically (F). See also Figures S1 and S3 .

    Journal: Cell

    Article Title: Dicer-like Enzymes with Sequence Cleavage Preferences

    doi: 10.1016/j.cell.2018.02.029

    Figure Lengend Snippet: In Vitro dsRNA Processing by Dcl4 (A) Expression profiles of four Dicer-like enzymes according to microarray data. y axis shows the expression level. x axis shows the developmental stages. (B) 15% polyacrylamide-urea gel with small RNAs 5′-labeled with 32 P. M is the small RNA ladder. No enzyme controls are marked as (−). “Beads only” is a no template RNA control for Dcl4. (C) Size distribution graphs of Dcl4 cleavage products based on sRNA sequencing. (D–F) 5′ nucleotide enrichment (D) and 3′ end nucleotide enrichment graph (E) for Dcl4 cleavage products showing relative abundance of the nucleotides. The sequence logos are shown for 25 nt sRNAs (F). A typical Dcl4 double-stranded cleavage product is shown schematically (F). See also Figures S1 and S3 .

    Article Snippet: RNA preparation DsRNA substrates were produced by T7 in vitro transcription with the MEGAscript T7 transcription kit (Thermo Scientific).

    Techniques: In Vitro, Expressing, Microarray, Labeling, Sequencing

    In Vitro dsRNA Processing by Dcl5 Using Templates Containing Paramecium MAC and IES Sequences (A) dsRNA templates used for in vitro assays with Dcl5 are shown schematically: a 500 nt long fragment of ND7 coding sequence (black line) and two templates made of three concatenated IESs (blue, purple, and orange). (B) 15% polyacrylamide-urea gel with small RNAs 5′-labeled with 32 P. M is the small RNA ladder. (+) and (−) indicate reactions performed with or without the enzyme. Dashed boxes indicate the bands corresponding to the most abundant products after sRNA sequencing. (C) Size distribution graphs of the cleavage products for Dcl5 with Concatemer and Concatemer Longer templates. (D and E) Enrichment graphs for the 5′ (D) and 3′ (E) ends of Dcl5 cleavage products.

    Journal: Cell

    Article Title: Dicer-like Enzymes with Sequence Cleavage Preferences

    doi: 10.1016/j.cell.2018.02.029

    Figure Lengend Snippet: In Vitro dsRNA Processing by Dcl5 Using Templates Containing Paramecium MAC and IES Sequences (A) dsRNA templates used for in vitro assays with Dcl5 are shown schematically: a 500 nt long fragment of ND7 coding sequence (black line) and two templates made of three concatenated IESs (blue, purple, and orange). (B) 15% polyacrylamide-urea gel with small RNAs 5′-labeled with 32 P. M is the small RNA ladder. (+) and (−) indicate reactions performed with or without the enzyme. Dashed boxes indicate the bands corresponding to the most abundant products after sRNA sequencing. (C) Size distribution graphs of the cleavage products for Dcl5 with Concatemer and Concatemer Longer templates. (D and E) Enrichment graphs for the 5′ (D) and 3′ (E) ends of Dcl5 cleavage products.

    Article Snippet: RNA preparation DsRNA substrates were produced by T7 in vitro transcription with the MEGAscript T7 transcription kit (Thermo Scientific).

    Techniques: In Vitro, Sequencing, Labeling