Structured Review

Unigene ssr loci
Wet laboratory validation of in silico identified <t>genic-SSR</t> length polymorphism between pigeonpea parental lines . Pigeonpea genic-SSR locus ASSR-8 showing: a. in silco polymorphism between the aligned <t>TSA</t> contigs of parental lines Asha (A) and UPAS 120 (U), b. agarose gel analysis of segregation of the ASSR-8 alleles in F 2 population. Positions of flanking primers and the polymorphic SSR sequence are highlighted.
Ssr Loci, supplied by Unigene, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ssr loci/product/Unigene
Average 90 stars, based on 2 article reviews
Price from $9.99 to $1999.99
ssr loci - by Bioz Stars, 2020-07
90/100 stars

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1) Product Images from "Development of genic-SSR markers by deep transcriptome sequencing in pigeonpea [Cajanus cajan (L.) Millspaugh]"

Article Title: Development of genic-SSR markers by deep transcriptome sequencing in pigeonpea [Cajanus cajan (L.) Millspaugh]

Journal: BMC Plant Biology

doi: 10.1186/1471-2229-11-17

Wet laboratory validation of in silico identified genic-SSR length polymorphism between pigeonpea parental lines . Pigeonpea genic-SSR locus ASSR-8 showing: a. in silco polymorphism between the aligned TSA contigs of parental lines Asha (A) and UPAS 120 (U), b. agarose gel analysis of segregation of the ASSR-8 alleles in F 2 population. Positions of flanking primers and the polymorphic SSR sequence are highlighted.
Figure Legend Snippet: Wet laboratory validation of in silico identified genic-SSR length polymorphism between pigeonpea parental lines . Pigeonpea genic-SSR locus ASSR-8 showing: a. in silco polymorphism between the aligned TSA contigs of parental lines Asha (A) and UPAS 120 (U), b. agarose gel analysis of segregation of the ASSR-8 alleles in F 2 population. Positions of flanking primers and the polymorphic SSR sequence are highlighted.

Techniques Used: In Silico, Agarose Gel Electrophoresis, Sequencing

Related Articles

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Article Title: Transcriptome Sequencing and Development of Genic SSR Markers of an Endangered Chinese Endemic Genus Dipteronia Oliver (Aceraceae)
Article Snippet: Detection of SSR Loci, Development and Validation of Microsatellite Markers In this study, a total of 6321/6757/12377 (D. sinensis /D. dyeriana /non-redundant unigenes) SSR loci were recognized using MISA perl script, of which 813/898/1658 unigene sequences contain more than one SSR.

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    Unigene est ssr loci
    Cluster analysis of 18 species of the Melilotus genus based on 70 <t>EST-SSR</t> markers.
    Est Ssr Loci, supplied by Unigene, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/est ssr loci/product/Unigene
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    est ssr loci - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    85
    Unigene polymorphic ssr loci
    Cluster analysis of 18 species of the Melilotus genus based on 70 <t>EST-SSR</t> markers.
    Polymorphic Ssr Loci, supplied by Unigene, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymorphic ssr loci/product/Unigene
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    polymorphic ssr loci - by Bioz Stars, 2020-07
    85/100 stars
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    Cluster analysis of 18 species of the Melilotus genus based on 70 EST-SSR markers.

    Journal: Scientific Reports

    Article Title: Cross-species transferability of EST-SSR markers developed from the transcriptome of Melilotus and their application to population genetics research

    doi: 10.1038/s41598-017-18049-8

    Figure Lengend Snippet: Cluster analysis of 18 species of the Melilotus genus based on 70 EST-SSR markers.

    Article Snippet: A total of 19,263 EST-SSR loci were detected from 104,358 unigene sequences, and 18,182 primer pairs were successfully designed.

    Techniques:

    Genetic structure of 54 individuals for 18 Melilotus species as inferred by STRUCTURE with the EST-SSR marker data set. Histogram of the STRUCTURE analysis for the model with K = 3 (showing the highest ΔK). The smallest vertical barre presents one individual. The assignment proportion of each individual into Cluster I, Cluster II and Cluster III is shown along the y-axis.

    Journal: Scientific Reports

    Article Title: Cross-species transferability of EST-SSR markers developed from the transcriptome of Melilotus and their application to population genetics research

    doi: 10.1038/s41598-017-18049-8

    Figure Lengend Snippet: Genetic structure of 54 individuals for 18 Melilotus species as inferred by STRUCTURE with the EST-SSR marker data set. Histogram of the STRUCTURE analysis for the model with K = 3 (showing the highest ΔK). The smallest vertical barre presents one individual. The assignment proportion of each individual into Cluster I, Cluster II and Cluster III is shown along the y-axis.

    Article Snippet: A total of 19,263 EST-SSR loci were detected from 104,358 unigene sequences, and 18,182 primer pairs were successfully designed.

    Techniques: Marker

    EST-SSR marker variations of 18 Melilotus species using primers 86, 170, 281 and 547. Each accession includes three individual plants; the letter ‘M’ denotes the molecular markers, which are 200 bp and 150 bp (top to bottom) with primer 86, primer 170, primer 281 and primer 547 (top to bottom).

    Journal: Scientific Reports

    Article Title: Cross-species transferability of EST-SSR markers developed from the transcriptome of Melilotus and their application to population genetics research

    doi: 10.1038/s41598-017-18049-8

    Figure Lengend Snippet: EST-SSR marker variations of 18 Melilotus species using primers 86, 170, 281 and 547. Each accession includes three individual plants; the letter ‘M’ denotes the molecular markers, which are 200 bp and 150 bp (top to bottom) with primer 86, primer 170, primer 281 and primer 547 (top to bottom).

    Article Snippet: A total of 19,263 EST-SSR loci were detected from 104,358 unigene sequences, and 18,182 primer pairs were successfully designed.

    Techniques: Marker

    Comparative electropherogram analysis of four EST-SSR loci (primers 21, 31, 61 and 392) among different accessions of M. albus . The primer 21 had trinucleotide repeats of (TTC) 4 , (TTC) 5 , and (TTC) 7 . (TCT) 4 , and (TCT) 7 were obtained using primer 31. Primer 61 can generate tandem repeats of (GATTA) 4 and (GATTA) 5 . (GA) 7 , (GA) 8 and (GA) 12 were obtained by using primer 392.

    Journal: Scientific Reports

    Article Title: Cross-species transferability of EST-SSR markers developed from the transcriptome of Melilotus and their application to population genetics research

    doi: 10.1038/s41598-017-18049-8

    Figure Lengend Snippet: Comparative electropherogram analysis of four EST-SSR loci (primers 21, 31, 61 and 392) among different accessions of M. albus . The primer 21 had trinucleotide repeats of (TTC) 4 , (TTC) 5 , and (TTC) 7 . (TCT) 4 , and (TCT) 7 were obtained using primer 31. Primer 61 can generate tandem repeats of (GATTA) 4 and (GATTA) 5 . (GA) 7 , (GA) 8 and (GA) 12 were obtained by using primer 392.

    Article Snippet: A total of 19,263 EST-SSR loci were detected from 104,358 unigene sequences, and 18,182 primer pairs were successfully designed.

    Techniques:

    Wet laboratory validation of in silico identified genic-SSR length polymorphism between pigeonpea parental lines . Pigeonpea genic-SSR locus ASSR-8 showing: a. in silco polymorphism between the aligned TSA contigs of parental lines Asha (A) and UPAS 120 (U), b. agarose gel analysis of segregation of the ASSR-8 alleles in F 2 population. Positions of flanking primers and the polymorphic SSR sequence are highlighted.

    Journal: BMC Plant Biology

    Article Title: Development of genic-SSR markers by deep transcriptome sequencing in pigeonpea [Cajanus cajan (L.) Millspaugh]

    doi: 10.1186/1471-2229-11-17

    Figure Lengend Snippet: Wet laboratory validation of in silico identified genic-SSR length polymorphism between pigeonpea parental lines . Pigeonpea genic-SSR locus ASSR-8 showing: a. in silco polymorphism between the aligned TSA contigs of parental lines Asha (A) and UPAS 120 (U), b. agarose gel analysis of segregation of the ASSR-8 alleles in F 2 population. Positions of flanking primers and the polymorphic SSR sequence are highlighted.

    Article Snippet: Frequency distribution of different types of genic-SSR loci A total of 3,771 SSR loci were identified in 3,327 TSA contigs, representing 7.6% of the total 43,324 unigene TSA contigs (Figure ).

    Techniques: In Silico, Agarose Gel Electrophoresis, Sequencing

    Outline of the process of unigene annotation and EST-SSR development in Actinidia eriantha .

    Journal: Frontiers in Plant Science

    Article Title: Development and Application of Transcriptome-Derived Microsatellites in Actinidia eriantha (Actinidiaceae)

    doi: 10.3389/fpls.2017.01383

    Figure Lengend Snippet: Outline of the process of unigene annotation and EST-SSR development in Actinidia eriantha .

    Article Snippet: A total of 8,658 EST-SSR loci were identified in 7,495 unigene sequences, for which primer pairs were successfully designed for 3,842 loci (44.4%).

    Techniques: