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Illumina Inc ssr loci
Statistical graphics of E . <t>ophiuroides</t> SSRs by the GMATA. (A) shows the distribution of <t>SSR</t> motifs, (B) shows grouped motif distribution, (C) shows Top distribution of SSR length.
Ssr Loci, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ssr loci/product/Illumina Inc
Average 91 stars, based on 13 article reviews
Price from $9.99 to $1999.99
ssr loci - by Bioz Stars, 2020-07
91/100 stars

Images

1) Product Images from "High-throughput SSR marker development and its application in a centipedegrass (Eremochloa ophiuroides (Munro) Hack.) genetic diversity analysis"

Article Title: High-throughput SSR marker development and its application in a centipedegrass (Eremochloa ophiuroides (Munro) Hack.) genetic diversity analysis

Journal: PLoS ONE

doi: 10.1371/journal.pone.0202605

Statistical graphics of E . ophiuroides SSRs by the GMATA. (A) shows the distribution of SSR motifs, (B) shows grouped motif distribution, (C) shows Top distribution of SSR length.
Figure Legend Snippet: Statistical graphics of E . ophiuroides SSRs by the GMATA. (A) shows the distribution of SSR motifs, (B) shows grouped motif distribution, (C) shows Top distribution of SSR length.

Techniques Used:

2) Product Images from "SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring. SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring"

Article Title: SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring. SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring

Journal: Ecology and Evolution

doi: 10.1002/ece3.4533

Flowchart of the laboratory procedure. (a) barcoding of primers: species A with n × 96 individuals genotyped at 20 loci, n : number of sets of barcoded primers; (b) multiplex PCRs of several loci per multiplex group (depicted here are 4 loci per multiplex group; 2–5 loci in the actual study); (c) pooling of the SSR amplicons of up to n individuals per sequencing library
Figure Legend Snippet: Flowchart of the laboratory procedure. (a) barcoding of primers: species A with n × 96 individuals genotyped at 20 loci, n : number of sets of barcoded primers; (b) multiplex PCRs of several loci per multiplex group (depicted here are 4 loci per multiplex group; 2–5 loci in the actual study); (c) pooling of the SSR amplicons of up to n individuals per sequencing library

Techniques Used: Multiplex Assay, Sequencing

Related Articles

Multiplex Assay:

Article Title: SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring. SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring
Article Snippet: .. Here, an NGS strategy is described that allows to genotype hundreds of individuals at many custom‐designed SSR loci simultaneously, combining multiplex PCR, barcoding, and Illumina sequencing. .. We created three different datasets for which alleles were coded according to (a) length of the repetitive region, (b) total fragment length, and (c) sequence identity, in order to evaluate the eventual benefits from having sequence data at hand, not only fragment length data.

Sequencing:

Article Title: SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring. SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring
Article Snippet: .. Initial detection of SSR loci relied on assembled Illumina paired‐end sequencing reads of cDNA transcripts, which in turn stemmed from RNA extracted from fresh plant or RNA‐later (Qiagen) treated material using the RNeasy Mini Kit (Qiagen). .. RNA extraction followed a standard protocol and included subsequent DNA digestion and an RNA re‐precipitation step.

Article Title: High-throughput SSR marker development and its application in a centipedegrass (Eremochloa ophiuroides (Munro) Hack.) genetic diversity analysis
Article Snippet: .. Recently, approximately 3,500 SSR primer pairs were successfully designed from 5,839 identified SSR loci based on Illumina paired-end sequencing reads [ ]. .. In the present study, a total of 48,061 SSR primer pairs were developed from assembled centipedegrass transcriptome sequences using an NGS-based RNA-seq technique.

Article Title: SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring. SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring
Article Snippet: .. Here, an NGS strategy is described that allows to genotype hundreds of individuals at many custom‐designed SSR loci simultaneously, combining multiplex PCR, barcoding, and Illumina sequencing. .. We created three different datasets for which alleles were coded according to (a) length of the repetitive region, (b) total fragment length, and (c) sequence identity, in order to evaluate the eventual benefits from having sequence data at hand, not only fragment length data.

Article Title: Development of Chloroplast Microsatellite Markers and Analysis of Chloroplast Diversity in Chinese Jujube (Ziziphus jujuba Mill.) and Wild Jujube (Ziziphus acidojujuba Mill.)
Article Snippet: .. In this study, the SSR loci were identified based on a draft chloroplast genome sequence of the jujube cultivar “Junzao,” which was assembled from sequence reads produced by an Illumina HiSeq 2000 platform, by sequencing the total DNA of young leaves. ..

RNA Sequencing Assay:

Article Title: High-throughput SSR marker development and its application in a centipedegrass (Eremochloa ophiuroides (Munro) Hack.) genetic diversity analysis
Article Snippet: .. Conclusions The present work represents a substantial advance in the identification of a large number of informative SSR loci in E . ophiuroides by high-throughput RNA sequencing technology based on the Illumina HiSeq 2000 platform. .. A total of 64,470 SSR loci were identified from the assembled transcriptome of E . ophiuroides .

Next-Generation Sequencing:

Article Title: SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring. SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring
Article Snippet: .. Here, an NGS strategy is described that allows to genotype hundreds of individuals at many custom‐designed SSR loci simultaneously, combining multiplex PCR, barcoding, and Illumina sequencing. .. We created three different datasets for which alleles were coded according to (a) length of the repetitive region, (b) total fragment length, and (c) sequence identity, in order to evaluate the eventual benefits from having sequence data at hand, not only fragment length data.

Produced:

Article Title: Development of Chloroplast Microsatellite Markers and Analysis of Chloroplast Diversity in Chinese Jujube (Ziziphus jujuba Mill.) and Wild Jujube (Ziziphus acidojujuba Mill.)
Article Snippet: .. In this study, the SSR loci were identified based on a draft chloroplast genome sequence of the jujube cultivar “Junzao,” which was assembled from sequence reads produced by an Illumina HiSeq 2000 platform, by sequencing the total DNA of young leaves. ..

Polymerase Chain Reaction:

Article Title: SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring. SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring
Article Snippet: .. Here, an NGS strategy is described that allows to genotype hundreds of individuals at many custom‐designed SSR loci simultaneously, combining multiplex PCR, barcoding, and Illumina sequencing. .. We created three different datasets for which alleles were coded according to (a) length of the repetitive region, (b) total fragment length, and (c) sequence identity, in order to evaluate the eventual benefits from having sequence data at hand, not only fragment length data.

other:

Article Title: High-throughput SSR marker development and its application in a centipedegrass (Eremochloa ophiuroides (Munro) Hack.) genetic diversity analysis
Article Snippet: A total of 64,470 SSR loci were identified from the assembled transcriptome of E . ophiuroides .

High Throughput Screening Assay:

Article Title: High-throughput SSR marker development and its application in a centipedegrass (Eremochloa ophiuroides (Munro) Hack.) genetic diversity analysis
Article Snippet: .. Conclusions The present work represents a substantial advance in the identification of a large number of informative SSR loci in E . ophiuroides by high-throughput RNA sequencing technology based on the Illumina HiSeq 2000 platform. .. A total of 64,470 SSR loci were identified from the assembled transcriptome of E . ophiuroides .

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    Illumina Inc ssr loci
    Statistical graphics of E . <t>ophiuroides</t> SSRs by the GMATA. (A) shows the distribution of <t>SSR</t> motifs, (B) shows grouped motif distribution, (C) shows Top distribution of SSR length.
    Ssr Loci, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ssr loci/product/Illumina Inc
    Average 91 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    ssr loci - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    93
    Illumina Inc custom designed ssr loci
    Flowchart of the laboratory procedure. (a) barcoding of primers: species A with n × 96 individuals genotyped at 20 loci, n : number of sets of <t>barcoded</t> primers; (b) multiplex PCRs of several loci per multiplex group (depicted here are 4 loci per multiplex group; 2–5 loci in the actual study); (c) pooling of the <t>SSR</t> amplicons of up to n individuals per sequencing library
    Custom Designed Ssr Loci, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/custom designed ssr loci/product/Illumina Inc
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    custom designed ssr loci - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Statistical graphics of E . ophiuroides SSRs by the GMATA. (A) shows the distribution of SSR motifs, (B) shows grouped motif distribution, (C) shows Top distribution of SSR length.

    Journal: PLoS ONE

    Article Title: High-throughput SSR marker development and its application in a centipedegrass (Eremochloa ophiuroides (Munro) Hack.) genetic diversity analysis

    doi: 10.1371/journal.pone.0202605

    Figure Lengend Snippet: Statistical graphics of E . ophiuroides SSRs by the GMATA. (A) shows the distribution of SSR motifs, (B) shows grouped motif distribution, (C) shows Top distribution of SSR length.

    Article Snippet: Conclusions The present work represents a substantial advance in the identification of a large number of informative SSR loci in E . ophiuroides by high-throughput RNA sequencing technology based on the Illumina HiSeq 2000 platform.

    Techniques:

    Flowchart of the laboratory procedure. (a) barcoding of primers: species A with n × 96 individuals genotyped at 20 loci, n : number of sets of barcoded primers; (b) multiplex PCRs of several loci per multiplex group (depicted here are 4 loci per multiplex group; 2–5 loci in the actual study); (c) pooling of the SSR amplicons of up to n individuals per sequencing library

    Journal: Ecology and Evolution

    Article Title: SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring. SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring

    doi: 10.1002/ece3.4533

    Figure Lengend Snippet: Flowchart of the laboratory procedure. (a) barcoding of primers: species A with n × 96 individuals genotyped at 20 loci, n : number of sets of barcoded primers; (b) multiplex PCRs of several loci per multiplex group (depicted here are 4 loci per multiplex group; 2–5 loci in the actual study); (c) pooling of the SSR amplicons of up to n individuals per sequencing library

    Article Snippet: Here, an NGS strategy is described that allows to genotype hundreds of individuals at many custom‐designed SSR loci simultaneously, combining multiplex PCR, barcoding, and Illumina sequencing.

    Techniques: Multiplex Assay, Sequencing

    Flowchart of the laboratory procedure. (a) barcoding of primers: species A with n × 96 individuals genotyped at 20 loci, n : number of sets of barcoded primers; (b) multiplex PCRs of several loci per multiplex group (depicted here are 4 loci per multiplex group; 2–5 loci in the actual study); (c) pooling of the SSR amplicons of up to n individuals per sequencing library

    Journal: Ecology and Evolution

    Article Title: SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring. SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring

    doi: 10.1002/ece3.4533

    Figure Lengend Snippet: Flowchart of the laboratory procedure. (a) barcoding of primers: species A with n × 96 individuals genotyped at 20 loci, n : number of sets of barcoded primers; (b) multiplex PCRs of several loci per multiplex group (depicted here are 4 loci per multiplex group; 2–5 loci in the actual study); (c) pooling of the SSR amplicons of up to n individuals per sequencing library

    Article Snippet: Here, an NGS strategy is described which allows to genotype hundreds of individuals at several, custom‐designed SSR loci simultaneously, using multiplex PCR and barcoded primers to separate individual‐specific Illumina sequence reads.

    Techniques: Multiplex Assay, Sequencing

    Flowchart of the laboratory procedure. (a) barcoding of primers: species A with n × 96 individuals genotyped at 20 loci, n : number of sets of barcoded primers; (b) multiplex PCRs of several loci per multiplex group (depicted here are 4 loci per multiplex group; 2–5 loci in the actual study); (c) pooling of the SSR amplicons of up to n individuals per sequencing library

    Journal: Ecology and Evolution

    Article Title: SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring. SSR‐seq: Genotyping of microsatellites using next‐generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring

    doi: 10.1002/ece3.4533

    Figure Lengend Snippet: Flowchart of the laboratory procedure. (a) barcoding of primers: species A with n × 96 individuals genotyped at 20 loci, n : number of sets of barcoded primers; (b) multiplex PCRs of several loci per multiplex group (depicted here are 4 loci per multiplex group; 2–5 loci in the actual study); (c) pooling of the SSR amplicons of up to n individuals per sequencing library

    Article Snippet: Here, an NGS strategy is described which allows to genotype hundreds of individuals at several, custom‐designed SSR loci simultaneously, using multiplex PCR and barcoded primers to separate individual‐specific Illumina sequence reads.

    Techniques: Multiplex Assay, Sequencing