symphony quartet peptide synthesizer  (Gyros Protein Technologies)


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    Name:
    Symphony Quartet 110 VAC 50 60Hz
    Description:
    Symphony Quartet Automated 4 Channel Solid Phase Peptide Synthesizer 110 VAC 50 60Hz Complete with computer Windows XP Symphony Windows software color flat screen monitor keyboard mouse printer cables and connectors PTI Predict Software set of 4 chemically resistant O rings safety shield solvent cabinet 20L pressure vessel single waste container solvent bottles 1 x 4L 1 x 500ml 3 x 1L
    Catalog Number:
    sqps-110
    Price:
    None
    Applications:
    Peptide Synthesis, Organic Synthesis
    Category:
    Instruments
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    Structured Review

    Gyros Protein Technologies symphony quartet peptide synthesizer
    Symphony Quartet Automated 4 Channel Solid Phase Peptide Synthesizer 110 VAC 50 60Hz Complete with computer Windows XP Symphony Windows software color flat screen monitor keyboard mouse printer cables and connectors PTI Predict Software set of 4 chemically resistant O rings safety shield solvent cabinet 20L pressure vessel single waste container solvent bottles 1 x 4L 1 x 500ml 3 x 1L
    https://www.bioz.com/result/symphony quartet peptide synthesizer/product/Gyros Protein Technologies
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    symphony quartet peptide synthesizer - by Bioz Stars, 2020-02
    93/100 stars

    Related Products / Commonly Used Together

    fmoc-protected d-amino acids
    rink amide mbha resin

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Broad-Spectrum Allosteric Inhibition of Herpesvirus Proteases
    Article Snippet: The P6 peptide substrate (PVYtBuQA-ACC) was purchased crude (AnaSpec, Inc.) and purified via reverse-phase high-performance liquid chromatography on a C18 column as described previously. .. The P4 peptide substrate, YtBuQA-ACC, was synthesized and purified as previously described, but using the Symphony Quartet multiple synthesizer (Protein Technologies, Inc.) for the addition of the last three amino acids.

    Article Title: Peptide length and prime-side sterics influence potency of peptide phosphonate protease inhibitors
    Article Snippet: The full-length peptide was synthesized using a Symphony Quartet peptide synthesizer (Protein Technologies, Inc.), acetylated with 8 equivalents each of acetic anhydride and DIPEA, and cleaved with 95% TFA/2.5% water/ 2.5% triisopropyl silane. .. Cleaved peptides were precipitated into cold ether, collected by centrifugation, and purified by HPLC.

    Article Title: Modified vaccinia Ankara expressing survivin combined with gemcitabine generates specific antitumor effects in a murine pancreatic carcinoma model
    Article Snippet: .. Peptides were synthesized in our laboratory with a Symphony Quartet peptide synthesizer (Protein Technologies Inc., Tucson, AZ) using standard FMOC protocols and purified to [95% purity by HPLC (Agilent 1200) using an Atlantis™ preparative HPLC column (Waters, Watertown, MA). .. The identity of the peptides was confirmed by MALDI TOF mass spectrometric analysis using a Kompact Probe mass spectrometer (Kratos Analytical, Shimadzu, Kyoto, Japan).

    Article Title: Pericyte seeded dual peptide scaffold with improved endothelialization for vascular graft tissue engineering
    Article Snippet: Peptides (Hep: CGGGAAALRKKLGKA and Adh: SVVYGLRGGC) were synthesized on a Symphony Quartet peptide synthesizer (Protein Technologies, Inc., Tucson, Arizona) using standard Fmoc chemistry on the solid-phase. .. The crude products were purified using a reverse phase preparative high performance liquid chromatography (HPLC, Shimadzu) running a mobile phase gradient of 95% deionized water and 5% acetonitrile (ACN) to 100% ACN with 0.1% (v/v) trifluoroacetic acid (TFA).

    Article Title: Peptide‐Functionalized Fluorescent Particles for In Situ Detection of Nitric Oxide via Peroxynitrite‐Mediated Nitration
    Article Snippet: Peptide Synthesis : Four synthetic peptides (P1: EKKDFY421 KDGKRL–CONH2 ; P2: GKRLKNY430 SLP–CONH2 ; P3: LHHSKHHAAY34 VNNLNV–CONH2 ; and P4: GGREYYY–CONH2 ; Figure S3, Supporting Information) were prepared either by manual solid‐phase peptide synthesis (SPPS) or on a peptide synthesizer (Symphony Quartet; Protein Technologies, Inc.) using standard Fmoc SPPS chemistry on a Rink‐amide MBHA resin. .. For purification, the peptide was dissolved in a solution of 4.9% ACN in ultrapure water with either 0.1% TFA or NH4 OH and purified using reverse‐phase preparative high‐performance liquid chromatography (HPLC; Shimadzu) running a mobile phase gradient of ultrapure water with 5% to 100% ACN containing 0.1% TFA or NH4 OH.

    Centrifugation:

    Article Title: Peptide length and prime-side sterics influence potency of peptide phosphonate protease inhibitors
    Article Snippet: The full-length peptide was synthesized using a Symphony Quartet peptide synthesizer (Protein Technologies, Inc.), acetylated with 8 equivalents each of acetic anhydride and DIPEA, and cleaved with 95% TFA/2.5% water/ 2.5% triisopropyl silane. .. Cleaved peptides were precipitated into cold ether, collected by centrifugation, and purified by HPLC.

    Recombinant:

    Article Title: Sensitive and Continuous Screening of Inhibitors of ?-Site Amyloid Precursor Protein Cleaving Enzyme 1 (BACE1) at Single SPR Chips
    Article Snippet: Recombinant human BACE1 was purchased from MP Biomedicals, LLC (Solon, OH). .. The peptide substrates for BACE1, CGGGKTEEISEVNLDAEFRHDSGY and H6 KTEEISEVNLDAEFRHDSGY and compound 3 were synthesized in house on an automatic peptide synthesizer (Symphony Quartet, Protein Technologies, Inc., Tucson, AZ).

    Synthesized:

    Article Title: Broad-Spectrum Allosteric Inhibition of Herpesvirus Proteases
    Article Snippet: .. The P4 peptide substrate, YtBuQA-ACC, was synthesized and purified as previously described, but using the Symphony Quartet multiple synthesizer (Protein Technologies, Inc.) for the addition of the last three amino acids. .. Protein Expression and Purification Expression and purification of the KSHV, HCMV, HSV-2, and EBV proteases and their respective isoleucine-to-valine and truncated variants were conducted as previously described., Primer sequences are listed in the .

    Article Title: Peptide length and prime-side sterics influence potency of peptide phosphonate protease inhibitors
    Article Snippet: .. The full-length peptide was synthesized using a Symphony Quartet peptide synthesizer (Protein Technologies, Inc.), acetylated with 8 equivalents each of acetic anhydride and DIPEA, and cleaved with 95% TFA/2.5% water/ 2.5% triisopropyl silane. .. Cleaved peptides were precipitated into cold ether, collected by centrifugation, and purified by HPLC.

    Article Title: Modified vaccinia Ankara expressing survivin combined with gemcitabine generates specific antitumor effects in a murine pancreatic carcinoma model
    Article Snippet: .. Peptides were synthesized in our laboratory with a Symphony Quartet peptide synthesizer (Protein Technologies Inc., Tucson, AZ) using standard FMOC protocols and purified to [95% purity by HPLC (Agilent 1200) using an Atlantis™ preparative HPLC column (Waters, Watertown, MA). .. The identity of the peptides was confirmed by MALDI TOF mass spectrometric analysis using a Kompact Probe mass spectrometer (Kratos Analytical, Shimadzu, Kyoto, Japan).

    Article Title: Sensitive and Continuous Screening of Inhibitors of ?-Site Amyloid Precursor Protein Cleaving Enzyme 1 (BACE1) at Single SPR Chips
    Article Snippet: .. The peptide substrates for BACE1, CGGGKTEEISEVNLDAEFRHDSGY and H6 KTEEISEVNLDAEFRHDSGY and compound 3 were synthesized in house on an automatic peptide synthesizer (Symphony Quartet, Protein Technologies, Inc., Tucson, AZ). .. It has been reported that the length of the peptide does not affect the enzymatic activity of BACE1 so long as the KTEEISEVNLDAEF sequence is included.

    Article Title: Global Identification of Biofilm-Specific Proteolysis in Candida albicans
    Article Snippet: The remaining substrates (IYRnHVQL, WPSnNKVG, and TFSYnRWP) were synthesized in house with a 7-methoxycoumarin (MCA) fluorophore and a 2,4-dinitrophenyl (DNP) quencher by standard 9-fluorenylmethoxycarbonyl (Fmoc) peptide synthesis chemistry. .. The first eight Fmoc-protected amino acids of each peptide were coupled to 150 mg of preloaded Fmoc-Lys(DNP) Wang resin (AnaSpec, Inc.) with a Symphony Quartet 4-channel peptide synthesizer (Protein Technologies, Inc.).

    Article Title: Pericyte seeded dual peptide scaffold with improved endothelialization for vascular graft tissue engineering
    Article Snippet: .. Peptides (Hep: CGGGAAALRKKLGKA and Adh: SVVYGLRGGC) were synthesized on a Symphony Quartet peptide synthesizer (Protein Technologies, Inc., Tucson, Arizona) using standard Fmoc chemistry on the solid-phase. .. The crude products were purified using a reverse phase preparative high performance liquid chromatography (HPLC, Shimadzu) running a mobile phase gradient of 95% deionized water and 5% acetonitrile (ACN) to 100% ACN with 0.1% (v/v) trifluoroacetic acid (TFA).

    Purification:

    Article Title: Broad-Spectrum Allosteric Inhibition of Herpesvirus Proteases
    Article Snippet: .. The P4 peptide substrate, YtBuQA-ACC, was synthesized and purified as previously described, but using the Symphony Quartet multiple synthesizer (Protein Technologies, Inc.) for the addition of the last three amino acids. .. Protein Expression and Purification Expression and purification of the KSHV, HCMV, HSV-2, and EBV proteases and their respective isoleucine-to-valine and truncated variants were conducted as previously described., Primer sequences are listed in the .

    Article Title: Peptide length and prime-side sterics influence potency of peptide phosphonate protease inhibitors
    Article Snippet: The full-length peptide was synthesized using a Symphony Quartet peptide synthesizer (Protein Technologies, Inc.), acetylated with 8 equivalents each of acetic anhydride and DIPEA, and cleaved with 95% TFA/2.5% water/ 2.5% triisopropyl silane. .. Cleaved peptides were precipitated into cold ether, collected by centrifugation, and purified by HPLC.

    Article Title: Self-sorting heterodimeric coiled coil peptides with defined and tuneable self-assembly properties
    Article Snippet: .. Peptide Synthesis and purification The peptides were obtained by solid phase peptide synthesis on a Symphony Quartet from Protein Technologies, Inc. As solid phase H-Rink-Amide ChemMatrix resin (0.47 mmol of NH2 /g of resin) was used on a 100 μM scale. .. Peptide Synthesis and purification The peptides were obtained by solid phase peptide synthesis on a Symphony Quartet from Protein Technologies, Inc. As solid phase H-Rink-Amide ChemMatrix resin (0.47 mmol of NH2 /g of resin) was used on a 100 μM scale.

    Article Title: Modified vaccinia Ankara expressing survivin combined with gemcitabine generates specific antitumor effects in a murine pancreatic carcinoma model
    Article Snippet: .. Peptides were synthesized in our laboratory with a Symphony Quartet peptide synthesizer (Protein Technologies Inc., Tucson, AZ) using standard FMOC protocols and purified to [95% purity by HPLC (Agilent 1200) using an Atlantis™ preparative HPLC column (Waters, Watertown, MA). .. The identity of the peptides was confirmed by MALDI TOF mass spectrometric analysis using a Kompact Probe mass spectrometer (Kratos Analytical, Shimadzu, Kyoto, Japan).

    Article Title: Sensitive and Continuous Screening of Inhibitors of ?-Site Amyloid Precursor Protein Cleaving Enzyme 1 (BACE1) at Single SPR Chips
    Article Snippet: The peptide substrates for BACE1, CGGGKTEEISEVNLDAEFRHDSGY and H6 KTEEISEVNLDAEFRHDSGY and compound 3 were synthesized in house on an automatic peptide synthesizer (Symphony Quartet, Protein Technologies, Inc., Tucson, AZ). .. The peptide substrates for BACE1, CGGGKTEEISEVNLDAEFRHDSGY and H6 KTEEISEVNLDAEFRHDSGY and compound 3 were synthesized in house on an automatic peptide synthesizer (Symphony Quartet, Protein Technologies, Inc., Tucson, AZ).

    Article Title: Pericyte seeded dual peptide scaffold with improved endothelialization for vascular graft tissue engineering
    Article Snippet: Peptides (Hep: CGGGAAALRKKLGKA and Adh: SVVYGLRGGC) were synthesized on a Symphony Quartet peptide synthesizer (Protein Technologies, Inc., Tucson, Arizona) using standard Fmoc chemistry on the solid-phase. .. The crude products were purified using a reverse phase preparative high performance liquid chromatography (HPLC, Shimadzu) running a mobile phase gradient of 95% deionized water and 5% acetonitrile (ACN) to 100% ACN with 0.1% (v/v) trifluoroacetic acid (TFA).

    Article Title: Peptide‐Functionalized Fluorescent Particles for In Situ Detection of Nitric Oxide via Peroxynitrite‐Mediated Nitration
    Article Snippet: Peptide Synthesis : Four synthetic peptides (P1: EKKDFY421 KDGKRL–CONH2 ; P2: GKRLKNY430 SLP–CONH2 ; P3: LHHSKHHAAY34 VNNLNV–CONH2 ; and P4: GGREYYY–CONH2 ; Figure S3, Supporting Information) were prepared either by manual solid‐phase peptide synthesis (SPPS) or on a peptide synthesizer (Symphony Quartet; Protein Technologies, Inc.) using standard Fmoc SPPS chemistry on a Rink‐amide MBHA resin. .. For purification, the peptide was dissolved in a solution of 4.9% ACN in ultrapure water with either 0.1% TFA or NH4 OH and purified using reverse‐phase preparative high‐performance liquid chromatography (HPLC; Shimadzu) running a mobile phase gradient of ultrapure water with 5% to 100% ACN containing 0.1% TFA or NH4 OH.

    Activity Assay:

    Article Title: Sensitive and Continuous Screening of Inhibitors of ?-Site Amyloid Precursor Protein Cleaving Enzyme 1 (BACE1) at Single SPR Chips
    Article Snippet: The peptide substrates for BACE1, CGGGKTEEISEVNLDAEFRHDSGY and H6 KTEEISEVNLDAEFRHDSGY and compound 3 were synthesized in house on an automatic peptide synthesizer (Symphony Quartet, Protein Technologies, Inc., Tucson, AZ). .. It has been reported that the length of the peptide does not affect the enzymatic activity of BACE1 so long as the KTEEISEVNLDAEF sequence is included.

    Mass Spectrometry:

    Article Title: Modified vaccinia Ankara expressing survivin combined with gemcitabine generates specific antitumor effects in a murine pancreatic carcinoma model
    Article Snippet: Peptides were synthesized in our laboratory with a Symphony Quartet peptide synthesizer (Protein Technologies Inc., Tucson, AZ) using standard FMOC protocols and purified to [95% purity by HPLC (Agilent 1200) using an Atlantis™ preparative HPLC column (Waters, Watertown, MA). .. The identity of the peptides was confirmed by MALDI TOF mass spectrometric analysis using a Kompact Probe mass spectrometer (Kratos Analytical, Shimadzu, Kyoto, Japan).

    Article Title: Pericyte seeded dual peptide scaffold with improved endothelialization for vascular graft tissue engineering
    Article Snippet: Peptides (Hep: CGGGAAALRKKLGKA and Adh: SVVYGLRGGC) were synthesized on a Symphony Quartet peptide synthesizer (Protein Technologies, Inc., Tucson, Arizona) using standard Fmoc chemistry on the solid-phase. .. The resulting pure products were confirmed by electrospray ionization mass spectrometry (ESI-MS) ( ).

    Sequencing:

    Article Title: Sensitive and Continuous Screening of Inhibitors of ?-Site Amyloid Precursor Protein Cleaving Enzyme 1 (BACE1) at Single SPR Chips
    Article Snippet: The peptide substrates for BACE1, CGGGKTEEISEVNLDAEFRHDSGY and H6 KTEEISEVNLDAEFRHDSGY and compound 3 were synthesized in house on an automatic peptide synthesizer (Symphony Quartet, Protein Technologies, Inc., Tucson, AZ). .. It has been reported that the length of the peptide does not affect the enzymatic activity of BACE1 so long as the KTEEISEVNLDAEF sequence is included.

    Reversed-phase Chromatography:

    Article Title: Sensitive and Continuous Screening of Inhibitors of ?-Site Amyloid Precursor Protein Cleaving Enzyme 1 (BACE1) at Single SPR Chips
    Article Snippet: The peptide substrates for BACE1, CGGGKTEEISEVNLDAEFRHDSGY and H6 KTEEISEVNLDAEFRHDSGY and compound 3 were synthesized in house on an automatic peptide synthesizer (Symphony Quartet, Protein Technologies, Inc., Tucson, AZ). .. The peptide substrates for BACE1, CGGGKTEEISEVNLDAEFRHDSGY and H6 KTEEISEVNLDAEFRHDSGY and compound 3 were synthesized in house on an automatic peptide synthesizer (Symphony Quartet, Protein Technologies, Inc., Tucson, AZ).

    Evaporation:

    Article Title: Peptide‐Functionalized Fluorescent Particles for In Situ Detection of Nitric Oxide via Peroxynitrite‐Mediated Nitration
    Article Snippet: Peptide Synthesis : Four synthetic peptides (P1: EKKDFY421 KDGKRL–CONH2 ; P2: GKRLKNY430 SLP–CONH2 ; P3: LHHSKHHAAY34 VNNLNV–CONH2 ; and P4: GGREYYY–CONH2 ; Figure S3, Supporting Information) were prepared either by manual solid‐phase peptide synthesis (SPPS) or on a peptide synthesizer (Symphony Quartet; Protein Technologies, Inc.) using standard Fmoc SPPS chemistry on a Rink‐amide MBHA resin. .. Amino acid couplings were carried out with Fmoc‐protected amino acids (4 equivalents), HBTU (3.75 equivalents), and DIEA (6 equivalents) in DMF for 1–2 h. The peptides were cleaved from the resin and deprotected with 95% trifluoroacetic acid (TFA), 2.5% tri‐isopropylsilane, and 2.5% distilled water (dH2 O) for 4 h. The TFA was removed using rotary evaporation, and the peptide was precipitated and washed with cold diethyl ether.

    Chromatin Immunoprecipitation:

    Article Title: Sensitive and Continuous Screening of Inhibitors of ?-Site Amyloid Precursor Protein Cleaving Enzyme 1 (BACE1) at Single SPR Chips
    Article Snippet: The peptide substrates for BACE1, CGGGKTEEISEVNLDAEFRHDSGY and H6 KTEEISEVNLDAEFRHDSGY and compound 3 were synthesized in house on an automatic peptide synthesizer (Symphony Quartet, Protein Technologies, Inc., Tucson, AZ). .. Insertion of the GGG segment between the substrate and the cysteine residue or use of a hexahistidine extension helps position the peptide substrate away from the SPR chip surface so that a higher BACE1 cleavage efficiency can be attained.

    SPR Assay:

    Article Title: Sensitive and Continuous Screening of Inhibitors of ?-Site Amyloid Precursor Protein Cleaving Enzyme 1 (BACE1) at Single SPR Chips
    Article Snippet: The peptide substrates for BACE1, CGGGKTEEISEVNLDAEFRHDSGY and H6 KTEEISEVNLDAEFRHDSGY and compound 3 were synthesized in house on an automatic peptide synthesizer (Symphony Quartet, Protein Technologies, Inc., Tucson, AZ). .. Insertion of the GGG segment between the substrate and the cysteine residue or use of a hexahistidine extension helps position the peptide substrate away from the SPR chip surface so that a higher BACE1 cleavage efficiency can be attained.

    Derivative Assay:

    Article Title: Modified vaccinia Ankara expressing survivin combined with gemcitabine generates specific antitumor effects in a murine pancreatic carcinoma model
    Article Snippet: A 15-mer overlapping peptide library derived from full-length mouse survivin was composed of 34 peptides, which covered all 140 amino acids of the survivin protein with an 11-amino acid overlap between peptides ( ). .. Peptides were synthesized in our laboratory with a Symphony Quartet peptide synthesizer (Protein Technologies Inc., Tucson, AZ) using standard FMOC protocols and purified to [95% purity by HPLC (Agilent 1200) using an Atlantis™ preparative HPLC column (Waters, Watertown, MA).

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  • 93
    Gyros Protein Technologies symphony quartet peptide synthesizer
    Symphony Quartet Peptide Synthesizer, supplied by Gyros Protein Technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/symphony quartet peptide synthesizer/product/Gyros Protein Technologies
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    symphony quartet peptide synthesizer - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

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