spri beads  (Beckman Coulter)


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    Structured Review

    Beckman Coulter spri beads
    Library preparation pipeline. <t>DNA</t> isolation is performed with a 96-head liquid handling robot. DNA fragmentation is achieved by sonication, either in glass tubes (Covaris) or PCR strips (Bandelin). <t>SPRI</t> bead cleanup is automated on a 96-head liquid handling robot. Three enzymatic steps for barcoded adapter ligation are performed by addition of enzyme (+ buffer), incubation, and heat inactivation in a thermocycler. After pooling of 48 barcoded libraries, samples are concentrated and size-selected using an E-gel. PCR is performed on the size-selected pool to enrich for adapter containing fragments and elongate them to full-length libraries. A final cleanup is performed in PCR strips mounted to a homemade magnetic stand.
    Spri Beads, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 98/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spri beads/product/Beckman Coulter
    Average 98 stars, based on 135 article reviews
    Price from $9.99 to $1999.99
    spri beads - by Bioz Stars, 2020-05
    98/100 stars

    Images

    1) Product Images from "Genotyping 1000 yeast strains by next-generation sequencing"

    Article Title: Genotyping 1000 yeast strains by next-generation sequencing

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-14-90

    Library preparation pipeline. DNA isolation is performed with a 96-head liquid handling robot. DNA fragmentation is achieved by sonication, either in glass tubes (Covaris) or PCR strips (Bandelin). SPRI bead cleanup is automated on a 96-head liquid handling robot. Three enzymatic steps for barcoded adapter ligation are performed by addition of enzyme (+ buffer), incubation, and heat inactivation in a thermocycler. After pooling of 48 barcoded libraries, samples are concentrated and size-selected using an E-gel. PCR is performed on the size-selected pool to enrich for adapter containing fragments and elongate them to full-length libraries. A final cleanup is performed in PCR strips mounted to a homemade magnetic stand.
    Figure Legend Snippet: Library preparation pipeline. DNA isolation is performed with a 96-head liquid handling robot. DNA fragmentation is achieved by sonication, either in glass tubes (Covaris) or PCR strips (Bandelin). SPRI bead cleanup is automated on a 96-head liquid handling robot. Three enzymatic steps for barcoded adapter ligation are performed by addition of enzyme (+ buffer), incubation, and heat inactivation in a thermocycler. After pooling of 48 barcoded libraries, samples are concentrated and size-selected using an E-gel. PCR is performed on the size-selected pool to enrich for adapter containing fragments and elongate them to full-length libraries. A final cleanup is performed in PCR strips mounted to a homemade magnetic stand.

    Techniques Used: DNA Extraction, Sonication, Polymerase Chain Reaction, Ligation, Incubation

    Related Articles

    Selection:

    Article Title: Contemporary ancestor? Adaptive divergence from standing genetic variation in Pacific marine threespine stickleback
    Article Snippet: .. Cleanup and size selection were performed simultaneously using SPRI beads (Beckman Coulter), at a bead ratio of 0.8× and 0.61× for left and right-side cleanup, respectively. ..

    Magnetic Beads:

    Article Title: Genotyping 1000 yeast strains by next-generation sequencing
    Article Snippet: .. DNA purification with SPRI beads The amplified DNA was purified in 0.2 ml PCR strips by mixing the DNA with 1x volume of Agencourt AMPure XP beads (Beckman Coulter) and select the magnetic beads with a homemade magnetic stand (Additional file : Figure S6). .. This stand consists of neodymium magnets (Webcraft GmbH, Gottmadingen, Germany) mounted on trimmed 96-well plates and can be used in combination with an 8-channel pipet.

    Purification:

    Article Title: Genotyping 1000 yeast strains by next-generation sequencing
    Article Snippet: .. DNA purification with SPRI beads The amplified DNA was purified in 0.2 ml PCR strips by mixing the DNA with 1x volume of Agencourt AMPure XP beads (Beckman Coulter) and select the magnetic beads with a homemade magnetic stand (Additional file : Figure S6). .. This stand consists of neodymium magnets (Webcraft GmbH, Gottmadingen, Germany) mounted on trimmed 96-well plates and can be used in combination with an 8-channel pipet.

    Article Title: Next-generation sequencing reveals novel differentially regulated mRNAs, lncRNAs, miRNAs, sdRNAs and a piRNA in pancreatic cancer
    Article Snippet: .. The libraries were amplified by PCR (KAPA HiFi Hot-Start Polymerase, KAPA Biosystems), purified by SPRI beads (Agencourt AMPure XP, Beckman Coulter) and sequenced (HiSeq2000, Illumina). .. Cell culture and transfection The pancreatic cancer cell line MiaPaCa was maintained at 37°C in a humified atmosphere of 5% CO2 and 95% air in Dulbecco’s modified Eagle Medium (Life Technologies, Inc, Darmstadt, Germany).

    Article Title: A scalable strategy for high-throughput GFP tagging of endogenous human proteins
    Article Snippet: .. Amplified dsDNA was purified using SPRI beads (AMPure XP resin, Beckman Coulter) at a 1:1 DNA:resin volume ratio (following manufacturer’s instructions) and eluted in 25 μL RNase-free H2 O. .. Next, RNA was formed by T7 in vitro transcription using T7 HiScribe reagents (New England Biolabs) in a 50-μL reaction containing: 5 pmol dsDNA template, 10 mM each NTP, and 5 μL HiScribe T7 polymerase.

    Article Title: Corrupted coordination of epigenetic modifications leads to diverging chromatin states and transcriptional heterogeneity in CLL
    Article Snippet: .. After amplification and purification using 0.8× SPRI beads, 0.5 ng cDNA was used for Nextera Tagmentation and library construction. .. Library quality and quantity was respectively assessed using Agilent Bioanalyzer 2100 and Qubit, respectively.

    Article Title: Novel and de novo mutations in pediatric refractory epilepsy
    Article Snippet: .. The captured DNAs were eluted, amplified and then their polymerase chain reaction (PCR) products were purified with SPRI beads (Beckman, USA). .. The enriched libraries were sequenced for paired-end reads of 150 bp by Illumina HiSeq X Ten.

    Incubation:

    Article Title: Evaluation of a solid matrix for collection and ambient storage of RNA from whole blood
    Article Snippet: .. After incubation, 700 μl of eluate was transferred to a new microcentrifuge tube and mixed with 400 μl of isopropanol and 10 μl SPRI beads from the Agencourt RNAdvance Blood kit (Beckman Coulter Genomics). .. RNA was then extracted following the manufacturer’s instruction; genomic DNA removal was performed using the Ambion DNase I kit (Ambion) at 37°C for 10 min.

    Amplification:

    Article Title: Genotyping 1000 yeast strains by next-generation sequencing
    Article Snippet: .. DNA purification with SPRI beads The amplified DNA was purified in 0.2 ml PCR strips by mixing the DNA with 1x volume of Agencourt AMPure XP beads (Beckman Coulter) and select the magnetic beads with a homemade magnetic stand (Additional file : Figure S6). .. This stand consists of neodymium magnets (Webcraft GmbH, Gottmadingen, Germany) mounted on trimmed 96-well plates and can be used in combination with an 8-channel pipet.

    Article Title: Next-generation sequencing reveals novel differentially regulated mRNAs, lncRNAs, miRNAs, sdRNAs and a piRNA in pancreatic cancer
    Article Snippet: .. The libraries were amplified by PCR (KAPA HiFi Hot-Start Polymerase, KAPA Biosystems), purified by SPRI beads (Agencourt AMPure XP, Beckman Coulter) and sequenced (HiSeq2000, Illumina). .. Cell culture and transfection The pancreatic cancer cell line MiaPaCa was maintained at 37°C in a humified atmosphere of 5% CO2 and 95% air in Dulbecco’s modified Eagle Medium (Life Technologies, Inc, Darmstadt, Germany).

    Article Title: A scalable strategy for high-throughput GFP tagging of endogenous human proteins
    Article Snippet: .. Amplified dsDNA was purified using SPRI beads (AMPure XP resin, Beckman Coulter) at a 1:1 DNA:resin volume ratio (following manufacturer’s instructions) and eluted in 25 μL RNase-free H2 O. .. Next, RNA was formed by T7 in vitro transcription using T7 HiScribe reagents (New England Biolabs) in a 50-μL reaction containing: 5 pmol dsDNA template, 10 mM each NTP, and 5 μL HiScribe T7 polymerase.

    Article Title: Corrupted coordination of epigenetic modifications leads to diverging chromatin states and transcriptional heterogeneity in CLL
    Article Snippet: .. After amplification and purification using 0.8× SPRI beads, 0.5 ng cDNA was used for Nextera Tagmentation and library construction. .. Library quality and quantity was respectively assessed using Agilent Bioanalyzer 2100 and Qubit, respectively.

    Article Title: Novel and de novo mutations in pediatric refractory epilepsy
    Article Snippet: .. The captured DNAs were eluted, amplified and then their polymerase chain reaction (PCR) products were purified with SPRI beads (Beckman, USA). .. The enriched libraries were sequenced for paired-end reads of 150 bp by Illumina HiSeq X Ten.

    DNA Purification:

    Article Title: Genotyping 1000 yeast strains by next-generation sequencing
    Article Snippet: .. DNA purification with SPRI beads The amplified DNA was purified in 0.2 ml PCR strips by mixing the DNA with 1x volume of Agencourt AMPure XP beads (Beckman Coulter) and select the magnetic beads with a homemade magnetic stand (Additional file : Figure S6). .. This stand consists of neodymium magnets (Webcraft GmbH, Gottmadingen, Germany) mounted on trimmed 96-well plates and can be used in combination with an 8-channel pipet.

    Polymerase Chain Reaction:

    Article Title: Genotyping 1000 yeast strains by next-generation sequencing
    Article Snippet: .. DNA purification with SPRI beads The amplified DNA was purified in 0.2 ml PCR strips by mixing the DNA with 1x volume of Agencourt AMPure XP beads (Beckman Coulter) and select the magnetic beads with a homemade magnetic stand (Additional file : Figure S6). .. This stand consists of neodymium magnets (Webcraft GmbH, Gottmadingen, Germany) mounted on trimmed 96-well plates and can be used in combination with an 8-channel pipet.

    Article Title: Next-generation sequencing reveals novel differentially regulated mRNAs, lncRNAs, miRNAs, sdRNAs and a piRNA in pancreatic cancer
    Article Snippet: .. The libraries were amplified by PCR (KAPA HiFi Hot-Start Polymerase, KAPA Biosystems), purified by SPRI beads (Agencourt AMPure XP, Beckman Coulter) and sequenced (HiSeq2000, Illumina). .. Cell culture and transfection The pancreatic cancer cell line MiaPaCa was maintained at 37°C in a humified atmosphere of 5% CO2 and 95% air in Dulbecco’s modified Eagle Medium (Life Technologies, Inc, Darmstadt, Germany).

    Article Title: Novel and de novo mutations in pediatric refractory epilepsy
    Article Snippet: .. The captured DNAs were eluted, amplified and then their polymerase chain reaction (PCR) products were purified with SPRI beads (Beckman, USA). .. The enriched libraries were sequenced for paired-end reads of 150 bp by Illumina HiSeq X Ten.

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
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  • 98
    Beckman Coulter spri beads
    Library preparation pipeline. <t>DNA</t> isolation is performed with a 96-head liquid handling robot. DNA fragmentation is achieved by sonication, either in glass tubes (Covaris) or PCR strips (Bandelin). <t>SPRI</t> bead cleanup is automated on a 96-head liquid handling robot. Three enzymatic steps for barcoded adapter ligation are performed by addition of enzyme (+ buffer), incubation, and heat inactivation in a thermocycler. After pooling of 48 barcoded libraries, samples are concentrated and size-selected using an E-gel. PCR is performed on the size-selected pool to enrich for adapter containing fragments and elongate them to full-length libraries. A final cleanup is performed in PCR strips mounted to a homemade magnetic stand.
    Spri Beads, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 98/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spri beads/product/Beckman Coulter
    Average 98 stars, based on 137 article reviews
    Price from $9.99 to $1999.99
    spri beads - by Bioz Stars, 2020-05
    98/100 stars
      Buy from Supplier

    88
    Beckman Coulter rnaclean spri beads
    Library preparation pipeline. <t>DNA</t> isolation is performed with a 96-head liquid handling robot. DNA fragmentation is achieved by sonication, either in glass tubes (Covaris) or PCR strips (Bandelin). <t>SPRI</t> bead cleanup is automated on a 96-head liquid handling robot. Three enzymatic steps for barcoded adapter ligation are performed by addition of enzyme (+ buffer), incubation, and heat inactivation in a thermocycler. After pooling of 48 barcoded libraries, samples are concentrated and size-selected using an E-gel. PCR is performed on the size-selected pool to enrich for adapter containing fragments and elongate them to full-length libraries. A final cleanup is performed in PCR strips mounted to a homemade magnetic stand.
    Rnaclean Spri Beads, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaclean spri beads/product/Beckman Coulter
    Average 88 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    rnaclean spri beads - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    Image Search Results


    Library preparation pipeline. DNA isolation is performed with a 96-head liquid handling robot. DNA fragmentation is achieved by sonication, either in glass tubes (Covaris) or PCR strips (Bandelin). SPRI bead cleanup is automated on a 96-head liquid handling robot. Three enzymatic steps for barcoded adapter ligation are performed by addition of enzyme (+ buffer), incubation, and heat inactivation in a thermocycler. After pooling of 48 barcoded libraries, samples are concentrated and size-selected using an E-gel. PCR is performed on the size-selected pool to enrich for adapter containing fragments and elongate them to full-length libraries. A final cleanup is performed in PCR strips mounted to a homemade magnetic stand.

    Journal: BMC Genomics

    Article Title: Genotyping 1000 yeast strains by next-generation sequencing

    doi: 10.1186/1471-2164-14-90

    Figure Lengend Snippet: Library preparation pipeline. DNA isolation is performed with a 96-head liquid handling robot. DNA fragmentation is achieved by sonication, either in glass tubes (Covaris) or PCR strips (Bandelin). SPRI bead cleanup is automated on a 96-head liquid handling robot. Three enzymatic steps for barcoded adapter ligation are performed by addition of enzyme (+ buffer), incubation, and heat inactivation in a thermocycler. After pooling of 48 barcoded libraries, samples are concentrated and size-selected using an E-gel. PCR is performed on the size-selected pool to enrich for adapter containing fragments and elongate them to full-length libraries. A final cleanup is performed in PCR strips mounted to a homemade magnetic stand.

    Article Snippet: DNA purification with SPRI beads The amplified DNA was purified in 0.2 ml PCR strips by mixing the DNA with 1x volume of Agencourt AMPure XP beads (Beckman Coulter) and select the magnetic beads with a homemade magnetic stand (Additional file : Figure S6).

    Techniques: DNA Extraction, Sonication, Polymerase Chain Reaction, Ligation, Incubation

    Representative bioanalyzer images of freshly isolated and bead-purified cfDNA. (A–C) Healthy human control, (D–F) WT mouse, and (G–I) PDAC mouse. The left columns depict the freshly isolated starting cfDNA (green arrow and dotted line) and the contaminating high molecular weight DNA (black arrow) evident by a wide peak. The middle column depicts the high molecular weight DNA removed from the same samples shown in (A–G) by the SPRI AMPure bead purification step after the 0.5X dilution step. The right column shows the purified cfDNA as recovered form the SPRI AMPure bead purification step after the 1.6X dilution step from the same samples shown in (A–G) . The desired cfDNA peak is visible ∼150–200 bp (green arrow and dotted line).

    Journal: Frontiers in Genetics

    Article Title: Development of a Method to Implement Whole-Genome Bisulfite Sequencing of cfDNA from Cancer Patients and a Mouse Tumor Model

    doi: 10.3389/fgene.2018.00006

    Figure Lengend Snippet: Representative bioanalyzer images of freshly isolated and bead-purified cfDNA. (A–C) Healthy human control, (D–F) WT mouse, and (G–I) PDAC mouse. The left columns depict the freshly isolated starting cfDNA (green arrow and dotted line) and the contaminating high molecular weight DNA (black arrow) evident by a wide peak. The middle column depicts the high molecular weight DNA removed from the same samples shown in (A–G) by the SPRI AMPure bead purification step after the 0.5X dilution step. The right column shows the purified cfDNA as recovered form the SPRI AMPure bead purification step after the 1.6X dilution step from the same samples shown in (A–G) . The desired cfDNA peak is visible ∼150–200 bp (green arrow and dotted line).

    Article Snippet: Isolation of cfDNA from Human and Mouse Plasma/Serum To isolate the cfDNA we utilized the Qiagen QIAamp Circulating Nucleic Acid kit (Cat no./ID: 55114) with the modifications described in Section “Results.” For cfDNA purification we compared Solid Phase Reversible Immobilisation beads (SPRI) from Beckman Coulter (Agencourt AMPure cat#A63880) with the DNA Clean & Concentrator kit, Zymo Research (D4003T).

    Techniques: Isolation, Purification, Molecular Weight