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splenocyte medium  (ZeptoMetrix corporation)
94
ZeptoMetrix corporation splenocyte medium
Figure 1. (Continued) G, XTT assay for 4T1 cancer cell proliferation following supplementation of the medium with UC intermediates (n = 3, two-way ANOVA). P values: 24 hours: aspartate: not significant, ammonia = 0.007, glutamate and fumarate <0.0001; 48 and 72 <0.0001 for all measurements. Metabolite concentrations were supplemented in the following concentrations: ammonia, 0.75 mmol/L as published (49); aspartate, 0.25 mmol/L; fuma- rate, 0.35 mmol/L; glutamate, 0.25 mmol/L. H, Ex vivo FACS analysis of CD8+ <t>splenocyte</t> cell survival (left) and activation (right) following supplementa- tion with 1 mmol/L ammonia (Amn; ref. 59; measured plasma ammonia levels 1.34 mmol/L; n = 5, Student t test); P = 0.011, 0.043, respectively. I, Heat map for differential gene expression in hepatocytes demonstrates a unique pattern on day 21 in the livers of 4T1 breast cancer mice compared with day 4. J, 4T1 day 21 vs. 4T1 day 4 pathway enrichment analysis. Each bar shows the fold enrichment of a specific pathway. TCA, tricarboxylic acid.
Splenocyte Medium, supplied by ZeptoMetrix corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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splenocyte complete medium  (Thermo Fisher)
99
Thermo Fisher splenocyte complete medium
Figure 1. (Continued) G, XTT assay for 4T1 cancer cell proliferation following supplementation of the medium with UC intermediates (n = 3, two-way ANOVA). P values: 24 hours: aspartate: not significant, ammonia = 0.007, glutamate and fumarate <0.0001; 48 and 72 <0.0001 for all measurements. Metabolite concentrations were supplemented in the following concentrations: ammonia, 0.75 mmol/L as published (49); aspartate, 0.25 mmol/L; fuma- rate, 0.35 mmol/L; glutamate, 0.25 mmol/L. H, Ex vivo FACS analysis of CD8+ <t>splenocyte</t> cell survival (left) and activation (right) following supplementa- tion with 1 mmol/L ammonia (Amn; ref. 59; measured plasma ammonia levels 1.34 mmol/L; n = 5, Student t test); P = 0.011, 0.043, respectively. I, Heat map for differential gene expression in hepatocytes demonstrates a unique pattern on day 21 in the livers of 4T1 breast cancer mice compared with day 4. J, 4T1 day 21 vs. 4T1 day 4 pathway enrichment analysis. Each bar shows the fold enrichment of a specific pathway. TCA, tricarboxylic acid.
Splenocyte Complete Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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viable splenocyte samples  (Cellular Technology Ltd)
97
Cellular Technology Ltd viable splenocyte samples
Figure 1. (Continued) G, XTT assay for 4T1 cancer cell proliferation following supplementation of the medium with UC intermediates (n = 3, two-way ANOVA). P values: 24 hours: aspartate: not significant, ammonia = 0.007, glutamate and fumarate <0.0001; 48 and 72 <0.0001 for all measurements. Metabolite concentrations were supplemented in the following concentrations: ammonia, 0.75 mmol/L as published (49); aspartate, 0.25 mmol/L; fuma- rate, 0.35 mmol/L; glutamate, 0.25 mmol/L. H, Ex vivo FACS analysis of CD8+ <t>splenocyte</t> cell survival (left) and activation (right) following supplementa- tion with 1 mmol/L ammonia (Amn; ref. 59; measured plasma ammonia levels 1.34 mmol/L; n = 5, Student t test); P = 0.011, 0.043, respectively. I, Heat map for differential gene expression in hepatocytes demonstrates a unique pattern on day 21 in the livers of 4T1 breast cancer mice compared with day 4. J, 4T1 day 21 vs. 4T1 day 4 pathway enrichment analysis. Each bar shows the fold enrichment of a specific pathway. TCA, tricarboxylic acid.
Viable Splenocyte Samples, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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splenocyte complete medium  (Cambrex)
90
Cambrex splenocyte complete medium
Figure 1. (Continued) G, XTT assay for 4T1 cancer cell proliferation following supplementation of the medium with UC intermediates (n = 3, two-way ANOVA). P values: 24 hours: aspartate: not significant, ammonia = 0.007, glutamate and fumarate <0.0001; 48 and 72 <0.0001 for all measurements. Metabolite concentrations were supplemented in the following concentrations: ammonia, 0.75 mmol/L as published (49); aspartate, 0.25 mmol/L; fuma- rate, 0.35 mmol/L; glutamate, 0.25 mmol/L. H, Ex vivo FACS analysis of CD8+ <t>splenocyte</t> cell survival (left) and activation (right) following supplementa- tion with 1 mmol/L ammonia (Amn; ref. 59; measured plasma ammonia levels 1.34 mmol/L; n = 5, Student t test); P = 0.011, 0.043, respectively. I, Heat map for differential gene expression in hepatocytes demonstrates a unique pattern on day 21 in the livers of 4T1 breast cancer mice compared with day 4. J, 4T1 day 21 vs. 4T1 day 4 pathway enrichment analysis. Each bar shows the fold enrichment of a specific pathway. TCA, tricarboxylic acid.
Splenocyte Complete Medium, supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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splenocyte medium  (Biochrom)
90
Biochrom splenocyte medium
Identification of IL-6 as a secreted factor influencing <t>splenocyte</t> migration. LPS stimulation was performed overnight in the presence of IL-6 antibody or a control antibody. The experiment was conducted in triplicates. The data were evaluated by a one-way ANOVA and a post hoc Tukey test in comparison to native + LPS (*** p ≤ 0.001) and native + LPS + control ab (### p ≤ 0.001). Other combinations were not significant in the one-way ANOVA and the following Tukey test
Splenocyte Medium, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rpmi-1640 complete medium for splenocyte culture  (Thermo Fisher)
90
Thermo Fisher rpmi-1640 complete medium for splenocyte culture
Identification of IL-6 as a secreted factor influencing <t>splenocyte</t> migration. LPS stimulation was performed overnight in the presence of IL-6 antibody or a control antibody. The experiment was conducted in triplicates. The data were evaluated by a one-way ANOVA and a post hoc Tukey test in comparison to native + LPS (*** p ≤ 0.001) and native + LPS + control ab (### p ≤ 0.001). Other combinations were not significant in the one-way ANOVA and the following Tukey test
Rpmi 1640 Complete Medium For Splenocyte Culture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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splenocyte/lymphocyte separation medium  (Dakewe Biotech Co)
90
Dakewe Biotech Co splenocyte/lymphocyte separation medium
Identification of IL-6 as a secreted factor influencing <t>splenocyte</t> migration. LPS stimulation was performed overnight in the presence of IL-6 antibody or a control antibody. The experiment was conducted in triplicates. The data were evaluated by a one-way ANOVA and a post hoc Tukey test in comparison to native + LPS (*** p ≤ 0.001) and native + LPS + control ab (### p ≤ 0.001). Other combinations were not significant in the one-way ANOVA and the following Tukey test
Splenocyte/Lymphocyte Separation Medium, supplied by Dakewe Biotech Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse splenocyte separation medium  (Dakewe Biotech Co)
90
Dakewe Biotech Co mouse splenocyte separation medium
Identification of IL-6 as a secreted factor influencing <t>splenocyte</t> migration. LPS stimulation was performed overnight in the presence of IL-6 antibody or a control antibody. The experiment was conducted in triplicates. The data were evaluated by a one-way ANOVA and a post hoc Tukey test in comparison to native + LPS (*** p ≤ 0.001) and native + LPS + control ab (### p ≤ 0.001). Other combinations were not significant in the one-way ANOVA and the following Tukey test
Mouse Splenocyte Separation Medium, supplied by Dakewe Biotech Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. (Continued) G, XTT assay for 4T1 cancer cell proliferation following supplementation of the medium with UC intermediates (n = 3, two-way ANOVA). P values: 24 hours: aspartate: not significant, ammonia = 0.007, glutamate and fumarate <0.0001; 48 and 72 <0.0001 for all measurements. Metabolite concentrations were supplemented in the following concentrations: ammonia, 0.75 mmol/L as published (49); aspartate, 0.25 mmol/L; fuma- rate, 0.35 mmol/L; glutamate, 0.25 mmol/L. H, Ex vivo FACS analysis of CD8+ splenocyte cell survival (left) and activation (right) following supplementa- tion with 1 mmol/L ammonia (Amn; ref. 59; measured plasma ammonia levels 1.34 mmol/L; n = 5, Student t test); P = 0.011, 0.043, respectively. I, Heat map for differential gene expression in hepatocytes demonstrates a unique pattern on day 21 in the livers of 4T1 breast cancer mice compared with day 4. J, 4T1 day 21 vs. 4T1 day 4 pathway enrichment analysis. Each bar shows the fold enrichment of a specific pathway. TCA, tricarboxylic acid.

Journal: Cancer Discovery

Article Title: Early Infiltration of Innate Immune Cells to the Liver Depletes HNF4α and Promotes Extrahepatic Carcinogenesis

doi: 10.1158/2159-8290.cd-22-1062

Figure Lengend Snippet: Figure 1. (Continued) G, XTT assay for 4T1 cancer cell proliferation following supplementation of the medium with UC intermediates (n = 3, two-way ANOVA). P values: 24 hours: aspartate: not significant, ammonia = 0.007, glutamate and fumarate <0.0001; 48 and 72 <0.0001 for all measurements. Metabolite concentrations were supplemented in the following concentrations: ammonia, 0.75 mmol/L as published (49); aspartate, 0.25 mmol/L; fuma- rate, 0.35 mmol/L; glutamate, 0.25 mmol/L. H, Ex vivo FACS analysis of CD8+ splenocyte cell survival (left) and activation (right) following supplementa- tion with 1 mmol/L ammonia (Amn; ref. 59; measured plasma ammonia levels 1.34 mmol/L; n = 5, Student t test); P = 0.011, 0.043, respectively. I, Heat map for differential gene expression in hepatocytes demonstrates a unique pattern on day 21 in the livers of 4T1 breast cancer mice compared with day 4. J, 4T1 day 21 vs. 4T1 day 4 pathway enrichment analysis. Each bar shows the fold enrichment of a specific pathway. TCA, tricarboxylic acid.

Article Snippet: Cells were resuspended in 2 × 106 cells/mL in splenocyte medium (complete RPMI medium supplemented with 50 μmol/L β-mercaptoethanol, 10% sodium pyruvate and nonessential amino acids) supplemented with 6,000 IU/mL IL2 (Chiron, rhIL2) and seeded in 24-well plates precoated with CD3 (BLG #100302).

Techniques: XTT Assay, Ex Vivo, Activation Assay, Clinical Proteomics, Gene Expression

Identification of IL-6 as a secreted factor influencing splenocyte migration. LPS stimulation was performed overnight in the presence of IL-6 antibody or a control antibody. The experiment was conducted in triplicates. The data were evaluated by a one-way ANOVA and a post hoc Tukey test in comparison to native + LPS (*** p ≤ 0.001) and native + LPS + control ab (### p ≤ 0.001). Other combinations were not significant in the one-way ANOVA and the following Tukey test

Journal: Molecular and Cellular Biochemistry

Article Title: The inflammatory signalling mediator TAK1 mediates lymphocyte recruitment to lipopolysaccharide-activated murine mesenchymal stem cells through interleukin-6

doi: 10.1007/s11010-021-04180-8

Figure Lengend Snippet: Identification of IL-6 as a secreted factor influencing splenocyte migration. LPS stimulation was performed overnight in the presence of IL-6 antibody or a control antibody. The experiment was conducted in triplicates. The data were evaluated by a one-way ANOVA and a post hoc Tukey test in comparison to native + LPS (*** p ≤ 0.001) and native + LPS + control ab (### p ≤ 0.001). Other combinations were not significant in the one-way ANOVA and the following Tukey test

Article Snippet: The cell pellet was suspended in 10 ml splenocyte medium (88% DMEM with 1 g/l glucose: Biochrom F 0415, 10% foetal calf serum (Thermo Fisher/ Invitrogen), 1% 100 × penicillin/ streptomycin mixture (Biochrom A2213), 2 mM glutamine (Biochrom K0283), 10 mM HEPES (Biochrom L1613), 500 μM β-mercaptoethanol (Invitrogen 31,350–010), 1 mM sodium pyruvate (Invitrogen 11,360–039) and 1% MEM non-essential amino acids (Invitrogen 11,140–035)).

Techniques: Migration, Control, Comparison