spio mscs  (Millipore)

 
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    Name:
    Mesenchymal Stem Cell Osteogenic Differentiation Medium
    Description:
    Mesenchymal Stem Cell Osteogenic Differentiation Medium Ready to use
    Catalog Number:
    C-28013
    Price:
    None
    Applications:
    Mesenchymal Stem Cell Osteogenic Differentiation Medium was designed for the directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue into osteogenic lineages. Recommended for:Human Mesenchymal Stem Cells from Bone Marrow (hMSC-BM)Human Mesenchymal Stem Cells from Umbilical Cord Matrix (hMSC-UC)Human Mesenchymal Stem Cells from Adipose Tissue (hMSC-AT)
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    Millipore spio mscs
    Mesenchymal Stem Cell Osteogenic Differentiation Medium
    Mesenchymal Stem Cell Osteogenic Differentiation Medium Ready to use
    https://www.bioz.com/result/spio mscs/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    spio mscs - by Bioz Stars, 2021-09
    99/100 stars

    Images

    1) Product Images from "Magnetic resonance hypointensive signal primarily originates from extracellular iron particles in the long-term tracking of mesenchymal stem cells transplanted in the infarcted myocardium"

    Article Title: Magnetic resonance hypointensive signal primarily originates from extracellular iron particles in the long-term tracking of mesenchymal stem cells transplanted in the infarcted myocardium

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S77858

    CMR imaging. Notes: ( A ) Representative in vivo CMR imaging of injected 5×10 5 unlabeled MSCs, ( B ) living SPIO-MSCs, ( C ) dead SPIO-MSCs, and ( D ) SPIO (0.6 μL Resovist) in swine heart. Red arrows in figures B – D indicate the signal void corresponding to the injection sites. ( E ) Quantitative analysis of signal intensity. ⊿SI=[(SI−SIunlabeled MSCs)/SIunlabeled MSCs]×100%. Abbreviations: CMR, cardiac magnetic resonance; LV, left ventricle; MSCs, mesenchymal stem cells; NS, not significant; RV, right ventricle; SPIO, superparamagnetic iron oxide; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide; SI, signal intensity.
    Figure Legend Snippet: CMR imaging. Notes: ( A ) Representative in vivo CMR imaging of injected 5×10 5 unlabeled MSCs, ( B ) living SPIO-MSCs, ( C ) dead SPIO-MSCs, and ( D ) SPIO (0.6 μL Resovist) in swine heart. Red arrows in figures B – D indicate the signal void corresponding to the injection sites. ( E ) Quantitative analysis of signal intensity. ⊿SI=[(SI−SIunlabeled MSCs)/SIunlabeled MSCs]×100%. Abbreviations: CMR, cardiac magnetic resonance; LV, left ventricle; MSCs, mesenchymal stem cells; NS, not significant; RV, right ventricle; SPIO, superparamagnetic iron oxide; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide; SI, signal intensity.

    Techniques Used: Imaging, In Vivo, Injection, Incubation

    Release rate of intracellular iron in vitro. Notes: ( A ) Prussian blue staining of SPIO-MSCs at different times after SPIO labeling (all figures in A are at 50 μm in size range). ( B ) Quantitative analysis showing that the intracellular mean iron load decreased continuously over time after magnetic labeling. ( C ) Electron microscopic images of MSCs at 4 days after labeling showed the release of iron dense particles (red arrow) from the cytoplasm. Abbreviations: d, days; SPIO, superparamagnetic iron oxide; MSCs, mesenchymal stem cells; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide.
    Figure Legend Snippet: Release rate of intracellular iron in vitro. Notes: ( A ) Prussian blue staining of SPIO-MSCs at different times after SPIO labeling (all figures in A are at 50 μm in size range). ( B ) Quantitative analysis showing that the intracellular mean iron load decreased continuously over time after magnetic labeling. ( C ) Electron microscopic images of MSCs at 4 days after labeling showed the release of iron dense particles (red arrow) from the cytoplasm. Abbreviations: d, days; SPIO, superparamagnetic iron oxide; MSCs, mesenchymal stem cells; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide.

    Techniques Used: In Vitro, Staining, Labeling, Incubation

    Proliferation, viability, and differentiation capacity of SPIO-MSCs. Notes: ( A ) Proliferation rate of SPIO-MSCs. ( B ) Viability rate of SPIO-MSCs. ( C ) Oil red O staining for adipogenic differentiation (both images in C are at 100 μm in size range). ( D ) RT-PCR for myocardial gene cTnT, Desmin , and α-cardiac actin . ( E ) Quantitative gene expression analysis. SPIO labeling did not affect the proliferation, viability, and differentiation capacity of MSCs. Abbreviations: MSCs, mesenchymal stem cells; SPIO, superparamagnetic iron oxide; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide; RT-PCR, real-time quantitative polymerase chain reaction; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; NS, not significant; NC, negative control; PC, positive control.
    Figure Legend Snippet: Proliferation, viability, and differentiation capacity of SPIO-MSCs. Notes: ( A ) Proliferation rate of SPIO-MSCs. ( B ) Viability rate of SPIO-MSCs. ( C ) Oil red O staining for adipogenic differentiation (both images in C are at 100 μm in size range). ( D ) RT-PCR for myocardial gene cTnT, Desmin , and α-cardiac actin . ( E ) Quantitative gene expression analysis. SPIO labeling did not affect the proliferation, viability, and differentiation capacity of MSCs. Abbreviations: MSCs, mesenchymal stem cells; SPIO, superparamagnetic iron oxide; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide; RT-PCR, real-time quantitative polymerase chain reaction; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; NS, not significant; NC, negative control; PC, positive control.

    Techniques Used: Staining, Reverse Transcription Polymerase Chain Reaction, Expressing, Labeling, Incubation, Real-time Polymerase Chain Reaction, Negative Control, Positive Control

    In vivo magnetic resonance imaging. Notes: ( A ) In vivo magnetic resonance imaging of tube-containing gel with 5×10 5 unlabeled MSCs, living SPIO-MSCs, dead SPIO-MSCs, and SPIO alone (0.6 μL Resovist) without MSCs, respectively. ( B ) Quantitative analysis of signal intensity. Equation for SI is ⊿SI = [(SI−SI unlabeled MSCs)/SI unlabeled MSCs] ×100%. Abbreviations: MSCs, mesenchymal stem cells; SPIO, superparamagnetic iron oxide; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide; SI, signal intensity; FSE, fast spin echo; T 1 WI, T1 weighted imaging; T 2 WI, T2 weighted imaging; T 2 *WI, T2 star weighted imaging.
    Figure Legend Snippet: In vivo magnetic resonance imaging. Notes: ( A ) In vivo magnetic resonance imaging of tube-containing gel with 5×10 5 unlabeled MSCs, living SPIO-MSCs, dead SPIO-MSCs, and SPIO alone (0.6 μL Resovist) without MSCs, respectively. ( B ) Quantitative analysis of signal intensity. Equation for SI is ⊿SI = [(SI−SI unlabeled MSCs)/SI unlabeled MSCs] ×100%. Abbreviations: MSCs, mesenchymal stem cells; SPIO, superparamagnetic iron oxide; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide; SI, signal intensity; FSE, fast spin echo; T 1 WI, T1 weighted imaging; T 2 WI, T2 weighted imaging; T 2 *WI, T2 star weighted imaging.

    Techniques Used: In Vivo, Magnetic Resonance Imaging, Incubation, Imaging

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    Article Snippet: .. Cell culture treatmentTo induce osteogenic differentiation, we used mineralization-inducing αMEM containing 100 μM/mL ascorbic acid and 2 mM β-glycerophosphate. ..

    Article Title: Mesenchymal stem cells-derived extracellular vesicles ameliorate Alzheimer’s disease in rat models via the microRNA-29c-3p/BACE1 axis and the Wnt/β-catenin pathway
    Article Snippet: .. In addition, other BM-MSCs of the 3rd generation were cultured in osteogenic medium (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) and adipogenic medium (Cyagen Biosciences, Santa Clara, CA, USA), and stained with alizarin red (Cyagen Biosciences) and Oil red O (Sigma-Aldrich) to analyze the osteogenic and adipogenic differentiation potential correspondingly. ..

    Staining:

    Article Title: Mesenchymal stem cells-derived extracellular vesicles ameliorate Alzheimer’s disease in rat models via the microRNA-29c-3p/BACE1 axis and the Wnt/β-catenin pathway
    Article Snippet: .. In addition, other BM-MSCs of the 3rd generation were cultured in osteogenic medium (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) and adipogenic medium (Cyagen Biosciences, Santa Clara, CA, USA), and stained with alizarin red (Cyagen Biosciences) and Oil red O (Sigma-Aldrich) to analyze the osteogenic and adipogenic differentiation potential correspondingly. ..

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    Cell Differentiation:

    Article Title: Pyrroline-5-Carboxylate Reductase 1 Directs the Cartilage Protective and Regenerative Potential of Murphy Roths Large Mouse Mesenchymal Stem Cells
    Article Snippet: .. Chondrogenic Differentiation of Mesenchymal Stem Cell Mesenchymal Stem Cell differentiation into chondrocytes was performed as previously described ( ). ..

    other:

    Article Title: The effect of parathyroid hormone on osteogenesis is mediated partly by osteolectin
    Article Snippet: To intermittently stimulate with PTH, PTH was added to the cultures (10 nM) for 1 h every day, then the medium was replaced with fresh osteogenic differentiation medium to wash out the PTH.

    Article Title: Psoralen accelerates osteogenic differentiation of human bone marrow mesenchymal stem cells by activating the TGF-β/Smad3 pathway
    Article Snippet: Third-passage hBMSCs were inoculated into a 6-well plate with different concentrations of psoralen (0.1, 1, 10 and 100 µmol/l) and osteogenic differentiation medium.

    Article Title: The effect of parathyroid hormone on osteogenesis is mediated partly by osteolectin
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  • 99
    Millipore spio mscs
    CMR imaging. Notes: ( A ) Representative in vivo CMR imaging of injected 5×10 5 unlabeled <t>MSCs,</t> ( B ) living <t>SPIO-MSCs,</t> ( C ) dead SPIO-MSCs, and ( D ) SPIO (0.6 μL Resovist) in swine heart. Red arrows in figures B – D indicate the signal void corresponding to the injection sites. ( E ) Quantitative analysis of signal intensity. ⊿SI=[(SI−SIunlabeled MSCs)/SIunlabeled MSCs]×100%. Abbreviations: CMR, cardiac magnetic resonance; LV, left ventricle; MSCs, mesenchymal stem cells; NS, not significant; RV, right ventricle; SPIO, superparamagnetic iron oxide; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide; SI, signal intensity.
    Spio Mscs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spio mscs/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    spio mscs - by Bioz Stars, 2021-09
    99/100 stars
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    98
    Millipore superparamagnetic iron oxide spio labeled cells mscs ngn1
    <t>MSCs/Ngn1</t> tracing by using <t>SPIO</t> labeling. A. MSCs/Ngn1 labeled with ferridex and protamine sulfate. Prussian blue staining for the identification of incorporation of ferridex into the MSCs/Ngn1. B. Distribution of MSCs/Ngn1 was monitored by SPIO labeling on MRI. Intra-arterial transplanted SPIO labeled MSCs/Ngn1 was widely distributed in the ischemic area. Each distribution pattern persisted during the serial MRI imaging studies on days 1, 4, and 7. C. Prussian blue staining 7 days after the ischemic brain transplantation with intra-arterial MSCs/Ngn1 cells. Immunostaining to identify transplanted SPIO labeled MSCs/Ngn1 cells and phagocytic activity (microglia/macrophages), by using anti hMT (human Mitochondria) and ED1 antibodies respectively, was performed in the ischemic boundary (I) and core (II) regions. SPIO correlated with hMT (red arrowheads) and ED1 (black arrowheads). Scale bars = 20 mm.
    Superparamagnetic Iron Oxide Spio Labeled Cells Mscs Ngn1, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superparamagnetic iron oxide spio labeled cells mscs ngn1/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superparamagnetic iron oxide spio labeled cells mscs ngn1 - by Bioz Stars, 2021-09
    98/100 stars
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    Image Search Results


    CMR imaging. Notes: ( A ) Representative in vivo CMR imaging of injected 5×10 5 unlabeled MSCs, ( B ) living SPIO-MSCs, ( C ) dead SPIO-MSCs, and ( D ) SPIO (0.6 μL Resovist) in swine heart. Red arrows in figures B – D indicate the signal void corresponding to the injection sites. ( E ) Quantitative analysis of signal intensity. ⊿SI=[(SI−SIunlabeled MSCs)/SIunlabeled MSCs]×100%. Abbreviations: CMR, cardiac magnetic resonance; LV, left ventricle; MSCs, mesenchymal stem cells; NS, not significant; RV, right ventricle; SPIO, superparamagnetic iron oxide; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide; SI, signal intensity.

    Journal: International Journal of Nanomedicine

    Article Title: Magnetic resonance hypointensive signal primarily originates from extracellular iron particles in the long-term tracking of mesenchymal stem cells transplanted in the infarcted myocardium

    doi: 10.2147/IJN.S77858

    Figure Lengend Snippet: CMR imaging. Notes: ( A ) Representative in vivo CMR imaging of injected 5×10 5 unlabeled MSCs, ( B ) living SPIO-MSCs, ( C ) dead SPIO-MSCs, and ( D ) SPIO (0.6 μL Resovist) in swine heart. Red arrows in figures B – D indicate the signal void corresponding to the injection sites. ( E ) Quantitative analysis of signal intensity. ⊿SI=[(SI−SIunlabeled MSCs)/SIunlabeled MSCs]×100%. Abbreviations: CMR, cardiac magnetic resonance; LV, left ventricle; MSCs, mesenchymal stem cells; NS, not significant; RV, right ventricle; SPIO, superparamagnetic iron oxide; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide; SI, signal intensity.

    Article Snippet: To determine the differentiation of myocardium-like cells, MSCs and SPIO-MSCs were cultured in culture medium with added 5-aza-2′-deoxycytidine (5-aza-C; Sigma-Aldrich Co) for 24 hours.

    Techniques: Imaging, In Vivo, Injection, Incubation

    Release rate of intracellular iron in vitro. Notes: ( A ) Prussian blue staining of SPIO-MSCs at different times after SPIO labeling (all figures in A are at 50 μm in size range). ( B ) Quantitative analysis showing that the intracellular mean iron load decreased continuously over time after magnetic labeling. ( C ) Electron microscopic images of MSCs at 4 days after labeling showed the release of iron dense particles (red arrow) from the cytoplasm. Abbreviations: d, days; SPIO, superparamagnetic iron oxide; MSCs, mesenchymal stem cells; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide.

    Journal: International Journal of Nanomedicine

    Article Title: Magnetic resonance hypointensive signal primarily originates from extracellular iron particles in the long-term tracking of mesenchymal stem cells transplanted in the infarcted myocardium

    doi: 10.2147/IJN.S77858

    Figure Lengend Snippet: Release rate of intracellular iron in vitro. Notes: ( A ) Prussian blue staining of SPIO-MSCs at different times after SPIO labeling (all figures in A are at 50 μm in size range). ( B ) Quantitative analysis showing that the intracellular mean iron load decreased continuously over time after magnetic labeling. ( C ) Electron microscopic images of MSCs at 4 days after labeling showed the release of iron dense particles (red arrow) from the cytoplasm. Abbreviations: d, days; SPIO, superparamagnetic iron oxide; MSCs, mesenchymal stem cells; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide.

    Article Snippet: To determine the differentiation of myocardium-like cells, MSCs and SPIO-MSCs were cultured in culture medium with added 5-aza-2′-deoxycytidine (5-aza-C; Sigma-Aldrich Co) for 24 hours.

    Techniques: In Vitro, Staining, Labeling, Incubation

    Proliferation, viability, and differentiation capacity of SPIO-MSCs. Notes: ( A ) Proliferation rate of SPIO-MSCs. ( B ) Viability rate of SPIO-MSCs. ( C ) Oil red O staining for adipogenic differentiation (both images in C are at 100 μm in size range). ( D ) RT-PCR for myocardial gene cTnT, Desmin , and α-cardiac actin . ( E ) Quantitative gene expression analysis. SPIO labeling did not affect the proliferation, viability, and differentiation capacity of MSCs. Abbreviations: MSCs, mesenchymal stem cells; SPIO, superparamagnetic iron oxide; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide; RT-PCR, real-time quantitative polymerase chain reaction; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; NS, not significant; NC, negative control; PC, positive control.

    Journal: International Journal of Nanomedicine

    Article Title: Magnetic resonance hypointensive signal primarily originates from extracellular iron particles in the long-term tracking of mesenchymal stem cells transplanted in the infarcted myocardium

    doi: 10.2147/IJN.S77858

    Figure Lengend Snippet: Proliferation, viability, and differentiation capacity of SPIO-MSCs. Notes: ( A ) Proliferation rate of SPIO-MSCs. ( B ) Viability rate of SPIO-MSCs. ( C ) Oil red O staining for adipogenic differentiation (both images in C are at 100 μm in size range). ( D ) RT-PCR for myocardial gene cTnT, Desmin , and α-cardiac actin . ( E ) Quantitative gene expression analysis. SPIO labeling did not affect the proliferation, viability, and differentiation capacity of MSCs. Abbreviations: MSCs, mesenchymal stem cells; SPIO, superparamagnetic iron oxide; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide; RT-PCR, real-time quantitative polymerase chain reaction; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; NS, not significant; NC, negative control; PC, positive control.

    Article Snippet: To determine the differentiation of myocardium-like cells, MSCs and SPIO-MSCs were cultured in culture medium with added 5-aza-2′-deoxycytidine (5-aza-C; Sigma-Aldrich Co) for 24 hours.

    Techniques: Staining, Reverse Transcription Polymerase Chain Reaction, Expressing, Labeling, Incubation, Real-time Polymerase Chain Reaction, Negative Control, Positive Control

    In vivo magnetic resonance imaging. Notes: ( A ) In vivo magnetic resonance imaging of tube-containing gel with 5×10 5 unlabeled MSCs, living SPIO-MSCs, dead SPIO-MSCs, and SPIO alone (0.6 μL Resovist) without MSCs, respectively. ( B ) Quantitative analysis of signal intensity. Equation for SI is ⊿SI = [(SI−SI unlabeled MSCs)/SI unlabeled MSCs] ×100%. Abbreviations: MSCs, mesenchymal stem cells; SPIO, superparamagnetic iron oxide; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide; SI, signal intensity; FSE, fast spin echo; T 1 WI, T1 weighted imaging; T 2 WI, T2 weighted imaging; T 2 *WI, T2 star weighted imaging.

    Journal: International Journal of Nanomedicine

    Article Title: Magnetic resonance hypointensive signal primarily originates from extracellular iron particles in the long-term tracking of mesenchymal stem cells transplanted in the infarcted myocardium

    doi: 10.2147/IJN.S77858

    Figure Lengend Snippet: In vivo magnetic resonance imaging. Notes: ( A ) In vivo magnetic resonance imaging of tube-containing gel with 5×10 5 unlabeled MSCs, living SPIO-MSCs, dead SPIO-MSCs, and SPIO alone (0.6 μL Resovist) without MSCs, respectively. ( B ) Quantitative analysis of signal intensity. Equation for SI is ⊿SI = [(SI−SI unlabeled MSCs)/SI unlabeled MSCs] ×100%. Abbreviations: MSCs, mesenchymal stem cells; SPIO, superparamagnetic iron oxide; SPIO-MSCs, mesenchymal stem cells incubated with superparamagnetic iron oxide; SI, signal intensity; FSE, fast spin echo; T 1 WI, T1 weighted imaging; T 2 WI, T2 weighted imaging; T 2 *WI, T2 star weighted imaging.

    Article Snippet: To determine the differentiation of myocardium-like cells, MSCs and SPIO-MSCs were cultured in culture medium with added 5-aza-2′-deoxycytidine (5-aza-C; Sigma-Aldrich Co) for 24 hours.

    Techniques: In Vivo, Magnetic Resonance Imaging, Incubation, Imaging

    MSCs/Ngn1 tracing by using SPIO labeling. A. MSCs/Ngn1 labeled with ferridex and protamine sulfate. Prussian blue staining for the identification of incorporation of ferridex into the MSCs/Ngn1. B. Distribution of MSCs/Ngn1 was monitored by SPIO labeling on MRI. Intra-arterial transplanted SPIO labeled MSCs/Ngn1 was widely distributed in the ischemic area. Each distribution pattern persisted during the serial MRI imaging studies on days 1, 4, and 7. C. Prussian blue staining 7 days after the ischemic brain transplantation with intra-arterial MSCs/Ngn1 cells. Immunostaining to identify transplanted SPIO labeled MSCs/Ngn1 cells and phagocytic activity (microglia/macrophages), by using anti hMT (human Mitochondria) and ED1 antibodies respectively, was performed in the ischemic boundary (I) and core (II) regions. SPIO correlated with hMT (red arrowheads) and ED1 (black arrowheads). Scale bars = 20 mm.

    Journal: Translational Neuroscience

    Article Title: Comparison of MSC-Neurogenin1 administration modality in MCAO rat model

    doi: 10.1515/tnsci-2016-0024

    Figure Lengend Snippet: MSCs/Ngn1 tracing by using SPIO labeling. A. MSCs/Ngn1 labeled with ferridex and protamine sulfate. Prussian blue staining for the identification of incorporation of ferridex into the MSCs/Ngn1. B. Distribution of MSCs/Ngn1 was monitored by SPIO labeling on MRI. Intra-arterial transplanted SPIO labeled MSCs/Ngn1 was widely distributed in the ischemic area. Each distribution pattern persisted during the serial MRI imaging studies on days 1, 4, and 7. C. Prussian blue staining 7 days after the ischemic brain transplantation with intra-arterial MSCs/Ngn1 cells. Immunostaining to identify transplanted SPIO labeled MSCs/Ngn1 cells and phagocytic activity (microglia/macrophages), by using anti hMT (human Mitochondria) and ED1 antibodies respectively, was performed in the ischemic boundary (I) and core (II) regions. SPIO correlated with hMT (red arrowheads) and ED1 (black arrowheads). Scale bars = 20 mm.

    Article Snippet: In vivo tracking of superparamagnetic iron oxide (SPIO)-labeled cells MSCs/Ngn1 were labeled using ferridex (Ferridex IV, TAEJOON Pharm, Republic of Korea) and protamine sulfate (Sigma-Aldrich, St. Louis, MO, USA) according to the procedure described by Arbab et al .

    Techniques: Labeling, Staining, Magnetic Resonance Imaging, Imaging, Transplantation Assay, Immunostaining, Activity Assay, HMT Assay