spike rna levels (Thermo Fisher)

Name:
ERCC RNA Spike In Mix
Description:
Variation in RNA expression data can be attributed to a variety of factors including the quality of the starting material the level of cellularity and RNA yield the platform employed and the person performing the experiment To control for these sources of variability a common set of external RNA controls has been developed by the External RNA Controls Consortium ERCC an ad hoc group of academic private and public organizations hosted by the National Institute of Standards and Technology NIST The controls consist of a set of unlabeled polyadenylated transcripts designed to be added to an RNA analysis experiment after sample isolation in order to measure against defined performance criteria Up until the design of such universally accepted controls it has been difficult to execute a thorough investigation of fundamental analytical performance metrics From the trusted brand of quality RNA reagents Ambion ERCC Spike In Control Mixes are commercially available pre formulated blends of 92 transcripts derived and traceable from NIST certified DNA plasmids The transcripts are designed to be 250 to 2 000 nt in length which mimic natural eukaryotic mRNAs Key product features Achieve a standard measure for data comparison across gene expression experiments• Measure sensitivity lower limit of detection and dynamic range of an experiment• Quantitate differential gene expressionUnlock the Potential of RNA AnalysisRNA analysis including gene expression profiling and whole transcriptome surveying can lead to better understanding of expression patterns in disease states and provides greater insights into biological pathways and molecular mechanisms that regulate cell fate development and disease progression Traditional methods of RNA analysis such as qRT PCR and microarrays are well established but are being replaced by next generation sequencing a high throughput digital alternative Because each method carries multiple platforms and with the need to compare various samples across platforms throughout the world a standard measure for data comparison is necessary As the capabilities of RNA analysis expand the necessity to create a standardized view of data will become even more important Achieve and Compare Results with Confirmed AccuracyAmbion ERCC RNA Spike In Controls are used to create a standard baseline measurement of RNA both within an experiment and across multiple experiments performed using various samples and platforms With two spike in mix formulations Figure 1 various measurements such as sensitivity or dynamic range can be examined to assess different parameters in an experiment or across experiments Figure 2 Furthermore expression fold change ratios between two samples can be calculated with a high degree of confidence using the highly concordant relationship between ExFold RNA Spike In 1 and ExFold RNA Spike In 2 Figure 3 Measurements are determined via known molar concentrations for each transcript within a spike in mix and through association of the two mixes using a combination of ratios across 4 different subgroups of the 92 transcripts The controls are ideal for next generation sequencing experiments such as on SOLiD System and supported microarray platforms such as the Illumina Sentrix BeadChip Choose Among Flexible Options for ERCC Kit ConfigurationsWhether measuring dynamic range or gene expression fold change Ambion ERCC Spike In Control Mixes are available in two kit configurations to meet your experimental needs Use the ERCC Spike In Mix to determine the dynamic range and lower limit of detection on your platform and use the ERCC ExFold Spike In Mixes to assess the accuracy of differential gene expression measurements ERCC RNA Spike In Mix 1 ExFold Spike In Mix 1 ExFold Spike In Mix 2 Nuclease free WaterERCC RNA Spike In Mix Cat No 4456740 10 µL 1 75 mLERCC ExFold RNA Spike In Mixes Cat No 4456739 10 µL10 µL1 75 mL Although ERCC RNA Spike In Mix 1 and ExFold Spike In Mix 1 contain the same formulation of ERCC transcripts do not substitute ERCC RNA Spike In Mix 1 for ExFold Spike In Mix 1 for fold change assessment Use only ExFold Spike In Mix 1 and Mix 2 with the same manufacturing lot number For Research Use Only Not for use in diagnostics procedures
Catalog Number:
4456740
Price:
None
Applications:
PCR & Real-Time PCR|RNA Sequencing|Real Time PCR (qPCR)|Real-Time PCR Primers, Probes, Arrays & Controls|SOLiD® Next-Generation Sequencing|Sample & Library Preparation for SOLiD® Next-Generation Sequencing|Whole Transcriptome Sequencing|Sequencing|Gene Expression Analysis & Genotyping|Microarray Hybridization & General Reagents
Category:
Standards Ladders Controls
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Structured Review
Variation in RNA expression data can be attributed to a variety of factors including the quality of the starting material the level of cellularity and RNA yield the platform employed and the person performing the experiment To control for these sources of variability a common set of external RNA controls has been developed by the External RNA Controls Consortium ERCC an ad hoc group of academic private and public organizations hosted by the National Institute of Standards and Technology NIST The controls consist of a set of unlabeled polyadenylated transcripts designed to be added to an RNA analysis experiment after sample isolation in order to measure against defined performance criteria Up until the design of such universally accepted controls it has been difficult to execute a thorough investigation of fundamental analytical performance metrics From the trusted brand of quality RNA reagents Ambion ERCC Spike In Control Mixes are commercially available pre formulated blends of 92 transcripts derived and traceable from NIST certified DNA plasmids The transcripts are designed to be 250 to 2 000 nt in length which mimic natural eukaryotic mRNAs Key product features Achieve a standard measure for data comparison across gene expression experiments• Measure sensitivity lower limit of detection and dynamic range of an experiment• Quantitate differential gene expressionUnlock the Potential of RNA AnalysisRNA analysis including gene expression profiling and whole transcriptome surveying can lead to better understanding of expression patterns in disease states and provides greater insights into biological pathways and molecular mechanisms that regulate cell fate development and disease progression Traditional methods of RNA analysis such as qRT PCR and microarrays are well established but are being replaced by next generation sequencing a high throughput digital alternative Because each method carries multiple platforms and with the need to compare various samples across platforms throughout the world a standard measure for data comparison is necessary As the capabilities of RNA analysis expand the necessity to create a standardized view of data will become even more important Achieve and Compare Results with Confirmed AccuracyAmbion ERCC RNA Spike In Controls are used to create a standard baseline measurement of RNA both within an experiment and across multiple experiments performed using various samples and platforms With two spike in mix formulations Figure 1 various measurements such as sensitivity or dynamic range can be examined to assess different parameters in an experiment or across experiments Figure 2 Furthermore expression fold change ratios between two samples can be calculated with a high degree of confidence using the highly concordant relationship between ExFold RNA Spike In 1 and ExFold RNA Spike In 2 Figure 3 Measurements are determined via known molar concentrations for each transcript within a spike in mix and through association of the two mixes using a combination of ratios across 4 different subgroups of the 92 transcripts The controls are ideal for next generation sequencing experiments such as on SOLiD System and supported microarray platforms such as the Illumina Sentrix BeadChip Choose Among Flexible Options for ERCC Kit ConfigurationsWhether measuring dynamic range or gene expression fold change Ambion ERCC Spike In Control Mixes are available in two kit configurations to meet your experimental needs Use the ERCC Spike In Mix to determine the dynamic range and lower limit of detection on your platform and use the ERCC ExFold Spike In Mixes to assess the accuracy of differential gene expression measurements ERCC RNA Spike In Mix 1 ExFold Spike In Mix 1 ExFold Spike In Mix 2 Nuclease free WaterERCC RNA Spike In Mix Cat No 4456740 10 µL 1 75 mLERCC ExFold RNA Spike In Mixes Cat No 4456739 10 µL10 µL1 75 mL Although ERCC RNA Spike In Mix 1 and ExFold Spike In Mix 1 contain the same formulation of ERCC transcripts do not substitute ERCC RNA Spike In Mix 1 for ExFold Spike In Mix 1 for fold change assessment Use only ExFold Spike In Mix 1 and Mix 2 with the same manufacturing lot number For Research Use Only Not for use in diagnostics procedures
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Amplification:Article Title: Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs Article Snippet: .. To eliminate genomic DNA contamination, 1 μL of genomic Magnetic Beads:Article Title: Patch-Seq Protocol to Analyze the Electrophysiology, Morphology and Transcriptome of Whole Single Neurons Derived From Human Pluripotent Stem Cells Article Snippet: .. simple • RNase AWAY Decontamination Reagent (Thermo Fisher Scientific, 10328011) simple • SMARTer Ultra Low Input RNA for Illumina Sequencing – High Volume kit (Clontech, 634828) Real-time Polymerase Chain Reaction:Article Title: Multiplexing polysome profiling experiments to study translation in Escherichia coli Article Snippet: .. To account for variability of the reverse transcription and the qPCR steps between samples and experiments, Sequencing:Article Title: Patch-Seq Protocol to Analyze the Electrophysiology, Morphology and Transcriptome of Whole Single Neurons Derived From Human Pluripotent Stem Cells Article Snippet: .. simple • RNase AWAY Decontamination Reagent (Thermo Fisher Scientific, 10328011) simple • SMARTer Ultra Low Input RNA for Illumina Sequencing – High Volume kit (Clontech, 634828) Lysis:Article Title: Reconstructing lineage hierarchies of the distal lung epithelium using single cell RNA-seq Article Snippet: .. Article Title: EXOSC10 sculpts the transcriptome during the growth-to-maturation transition in mouse oocytes Article Snippet: .. Briefly, 1 μl of 105-fold diluted Polymerase Chain Reaction:Article Title: Patch-Seq Protocol to Analyze the Electrophysiology, Morphology and Transcriptome of Whole Single Neurons Derived From Human Pluripotent Stem Cells Article Snippet: .. simple • RNase AWAY Decontamination Reagent (Thermo Fisher Scientific, 10328011) simple • SMARTer Ultra Low Input RNA for Illumina Sequencing – High Volume kit (Clontech, 634828) Staining:Article Title: Patch-Seq Protocol to Analyze the Electrophysiology, Morphology and Transcriptome of Whole Single Neurons Derived From Human Pluripotent Stem Cells Article Snippet: .. simple • RNase AWAY Decontamination Reagent (Thermo Fisher Scientific, 10328011) simple • SMARTer Ultra Low Input RNA for Illumina Sequencing – High Volume kit (Clontech, 634828) |