spei  (New England Biolabs)


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    New England Biolabs spei
    Spei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    spei - by Bioz Stars, 2023-03
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    spei  (New England Biolabs)


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    New England Biolabs spei
    Spei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    spei  (New England Biolabs)


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    New England Biolabs spei
    Spei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    spei  (New England Biolabs)


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    New England Biolabs spei
    Spei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    spei hf  (New England Biolabs)


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    New England Biolabs spei hf
    Spei Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    spei  (New England Biolabs)


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    New England Biolabs spei
    Spei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    spei  (New England Biolabs)


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    New England Biolabs spei
    Spei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    spei digested pml107  (New England Biolabs)


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    New England Biolabs spei digested pml107
    Spei Digested Pml107, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    spei  (New England Biolabs)


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    New England Biolabs spei
    Spei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    restriction enzyme spei  (New England Biolabs)


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    New England Biolabs restriction enzyme spei
    Restriction Enzyme Spei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    spei hf  (New England Biolabs)


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    New England Biolabs spei hf
    a Schematic depicting the construction of a bioluminescent reporter that encodes the entire Pl lux cassette under control of the Bt rpoD promoter and rpiL* RBS (top); a Bacteroides-optimized lux cassette with rearranged luxA -E (indicated by the shaded regions) and Bt intergenic regions from a constitutively expressed Bt operon (BT1160-1155; middle); or <t>p</t> <t>Bolux</t> which has BamHI and <t>SpeI</t> sites positioned upstream of the Bacteroides-optimized lux cassette (bottom) in the multi-copy plasmid pLYL01. b Relative luminescence (solid lines) or growth (dashed lines) from Bt strains harboring an empty vector (black) or plasmids containing either the lux operon from Pl (p lux-Pl , blue) or the Bacteroides-optimized lux cassette (p lux-Bt , red) expressed from the Bt rpoD promoter and rpiL* RBS were measured during growth in minimal media containing 0.5% galactose as the sole carbon source. c The relative luminescence (solid lines) or growth (dashed lines) of Bt strains harboring empty p Bolux (black) or a plasmid with the Bt rpoD promoter cloned into the BamHI and SpeI sites (red) during growth in galactose as the sole carbon source. All values in b , c are the mean of 8 biological replicates and error bars are SEM in color-matched shading. Source data are provided as a Source Data file.
    Spei Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Harnessing gut microbes for glycan detection and quantification"

    Article Title: Harnessing gut microbes for glycan detection and quantification

    Journal: Nature Communications

    doi: 10.1038/s41467-022-35626-2

    a Schematic depicting the construction of a bioluminescent reporter that encodes the entire Pl lux cassette under control of the Bt rpoD promoter and rpiL* RBS (top); a Bacteroides-optimized lux cassette with rearranged luxA -E (indicated by the shaded regions) and Bt intergenic regions from a constitutively expressed Bt operon (BT1160-1155; middle); or p Bolux which has BamHI and SpeI sites positioned upstream of the Bacteroides-optimized lux cassette (bottom) in the multi-copy plasmid pLYL01. b Relative luminescence (solid lines) or growth (dashed lines) from Bt strains harboring an empty vector (black) or plasmids containing either the lux operon from Pl (p lux-Pl , blue) or the Bacteroides-optimized lux cassette (p lux-Bt , red) expressed from the Bt rpoD promoter and rpiL* RBS were measured during growth in minimal media containing 0.5% galactose as the sole carbon source. c The relative luminescence (solid lines) or growth (dashed lines) of Bt strains harboring empty p Bolux (black) or a plasmid with the Bt rpoD promoter cloned into the BamHI and SpeI sites (red) during growth in galactose as the sole carbon source. All values in b , c are the mean of 8 biological replicates and error bars are SEM in color-matched shading. Source data are provided as a Source Data file.
    Figure Legend Snippet: a Schematic depicting the construction of a bioluminescent reporter that encodes the entire Pl lux cassette under control of the Bt rpoD promoter and rpiL* RBS (top); a Bacteroides-optimized lux cassette with rearranged luxA -E (indicated by the shaded regions) and Bt intergenic regions from a constitutively expressed Bt operon (BT1160-1155; middle); or p Bolux which has BamHI and SpeI sites positioned upstream of the Bacteroides-optimized lux cassette (bottom) in the multi-copy plasmid pLYL01. b Relative luminescence (solid lines) or growth (dashed lines) from Bt strains harboring an empty vector (black) or plasmids containing either the lux operon from Pl (p lux-Pl , blue) or the Bacteroides-optimized lux cassette (p lux-Bt , red) expressed from the Bt rpoD promoter and rpiL* RBS were measured during growth in minimal media containing 0.5% galactose as the sole carbon source. c The relative luminescence (solid lines) or growth (dashed lines) of Bt strains harboring empty p Bolux (black) or a plasmid with the Bt rpoD promoter cloned into the BamHI and SpeI sites (red) during growth in galactose as the sole carbon source. All values in b , c are the mean of 8 biological replicates and error bars are SEM in color-matched shading. Source data are provided as a Source Data file.

    Techniques Used: Plasmid Preparation, Clone Assay

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