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Molecular Devices LLC spectrofluorometer
Spectrofluorometer, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spectrofluorometer/product/Molecular Devices LLC
Average 92 stars, based on 9 article reviews
Price from $9.99 to $1999.99
spectrofluorometer - by Bioz Stars, 2020-09
92/100 stars

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Article Title: Molecular sled is an eleven-amino acid vehicle facilitating biochemical interactions via sliding components along DNA
Article Snippet: .. Fluorescence anisotropy Steady-state fluorescence anisotropy measurements were performed using an ISS model PC-1 photon counting spectrofluorometer (ISS, Champaign, IL) equipped with polarization accessories or a SpectraMax M5 plate reader from Molecular Devices. ..

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  • 88
    Molecular Devices LLC spectramax m2 spectrofluorometer
    Dependence of bioprobe BRET H ratio (500 nm/470 nm) on thrombin pre-treatment (mean ± S.D., n = 3). All treatments included 1 µM GFP 2 -RG-RLuc. The control was 1 µM RLuc, 1 µM GFP 2 and 5 µM native coelenterazine. Thrombin pre-treatment was 54 nM thrombin for 90 minutes at 30°C. The thrombin+Hirudin condition involved addition of 2 units of hirudin at room temperature 10 minutes before addition of 54 nM thrombin. All data were measured in a 96 well microplate using a <t>SpectraMax</t> M2 spectrofluorometer.
    Spectramax M2 Spectrofluorometer, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spectramax m2 spectrofluorometer/product/Molecular Devices LLC
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    spectramax m2 spectrofluorometer - by Bioz Stars, 2020-09
    88/100 stars
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    89
    Molecular Devices LLC gemini em microplate spectrofluorometer
    Inhibition of HDAC activity by active chalcone derivatives. Total protein extracts from K562 cells were incubated with vehicle (0) or various concentrations of the (A) chalcone 10 (B) chalcone 12 (C) chalcone 15 or (D) chalcone 21 for 1 h in the presence of an HDAC fluorometric substrate. Fluorescence was measured using a Gemini EM <t>microplate</t> spectrofluorometer and normalized by the vehicle-treated control enzyme activities. Vehicle-treated control corresponds to 0 on the chart. Results are presented as a mean ± SD of at least three independent experiments.
    Gemini Em Microplate Spectrofluorometer, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 89/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gemini em microplate spectrofluorometer/product/Molecular Devices LLC
    Average 89 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    gemini em microplate spectrofluorometer - by Bioz Stars, 2020-09
    89/100 stars
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    92
    Molecular Devices LLC microplate spectrofluorometer
    Pxn-KD fails to increase tyrosine phosphorylation and calcium mobilization. (A) Washed platelets obtained from control and Pxn-KD experiments were stimulated with 150 ng/mL convulxin for the indicated times. The cell lysates were resolved by SDS-polyacrylamide gel electrophoresis and then immunoblotted with an anti-phosphotyrosine mAb (4G10). The data shown are representative of three independent experiments. (B) Control and Pxn-KD platelets were labeled with GFP-Certified™ FluoForte™ dye. Changes in intracellular calcium levels after stimulation with an indicated concentration of AYPGKF or convulxin were then measured every 30 s. Data are expressed as the relative fluorescence unit (RFU) measured using a <t>microplate</t> spectrofluorometer (excitation, 530 nm; emission, 570 nm). The peak calcium concentration was measured after stimulation (open bars: control platelets; black bars: Pxn-KD platelets). Columns and error bars represent the mean ± s.d. ( n = 5–8). Statistical significance was determined by the Student’s t -test. (C) Control and Pxn-KD platelets were pretreated with 1 mmol/L EDTA and/or 20 μmol/L BAPTA-AM for 10 min, and then stimulated with or without 1 mmol/L AYPGKF or 150 ng/mL convulxin. P-selectin expression on GFP-positive platelets was determined by flow cytometry. Columns and error bars represent the mean ± s.d. of P-selectin expression (n = 4).
    Microplate Spectrofluorometer, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 92/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microplate spectrofluorometer/product/Molecular Devices LLC
    Average 92 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    microplate spectrofluorometer - by Bioz Stars, 2020-09
    92/100 stars
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    85
    Molecular Devices LLC spectra max gemini microplate spectrofluorometer
    Validation of the method with the three known genotypes of the ALDH2 gene (C/C, C/T, T/T). After the SBE reaction, the fluorescence emission spectra (580–700 nm) were measured with a <t>microplate</t> fluorescence reader. The NTC spectrum was subtracted from all spectra.
    Spectra Max Gemini Microplate Spectrofluorometer, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spectra max gemini microplate spectrofluorometer/product/Molecular Devices LLC
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    spectra max gemini microplate spectrofluorometer - by Bioz Stars, 2020-09
    85/100 stars
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    Dependence of bioprobe BRET H ratio (500 nm/470 nm) on thrombin pre-treatment (mean ± S.D., n = 3). All treatments included 1 µM GFP 2 -RG-RLuc. The control was 1 µM RLuc, 1 µM GFP 2 and 5 µM native coelenterazine. Thrombin pre-treatment was 54 nM thrombin for 90 minutes at 30°C. The thrombin+Hirudin condition involved addition of 2 units of hirudin at room temperature 10 minutes before addition of 54 nM thrombin. All data were measured in a 96 well microplate using a SpectraMax M2 spectrofluorometer.

    Journal: PLoS ONE

    Article Title: Comparison of Static and Microfluidic Protease Assays Using Modified Bioluminescence Resonance Energy Transfer Chemistry

    doi: 10.1371/journal.pone.0088399

    Figure Lengend Snippet: Dependence of bioprobe BRET H ratio (500 nm/470 nm) on thrombin pre-treatment (mean ± S.D., n = 3). All treatments included 1 µM GFP 2 -RG-RLuc. The control was 1 µM RLuc, 1 µM GFP 2 and 5 µM native coelenterazine. Thrombin pre-treatment was 54 nM thrombin for 90 minutes at 30°C. The thrombin+Hirudin condition involved addition of 2 units of hirudin at room temperature 10 minutes before addition of 54 nM thrombin. All data were measured in a 96 well microplate using a SpectraMax M2 spectrofluorometer.

    Article Snippet: Experimental Set-up Simultaneous dual emission hybrid BRET measurements were carried out in a microplate using a SpectraMax M2 spectrofluorometer (Molecular Devices) in luminescence scan mode between wavelengths of 400 to 650 nm and in the microfluidics apparatus described below.

    Techniques: Bioluminescence Resonance Energy Transfer

    Inhibition of HDAC activity by active chalcone derivatives. Total protein extracts from K562 cells were incubated with vehicle (0) or various concentrations of the (A) chalcone 10 (B) chalcone 12 (C) chalcone 15 or (D) chalcone 21 for 1 h in the presence of an HDAC fluorometric substrate. Fluorescence was measured using a Gemini EM microplate spectrofluorometer and normalized by the vehicle-treated control enzyme activities. Vehicle-treated control corresponds to 0 on the chart. Results are presented as a mean ± SD of at least three independent experiments.

    Journal: Oncology Reports

    Article Title: Natural chalcones as dual inhibitors of HDACs and NF-?B

    doi: 10.3892/or.2012.1870

    Figure Lengend Snippet: Inhibition of HDAC activity by active chalcone derivatives. Total protein extracts from K562 cells were incubated with vehicle (0) or various concentrations of the (A) chalcone 10 (B) chalcone 12 (C) chalcone 15 or (D) chalcone 21 for 1 h in the presence of an HDAC fluorometric substrate. Fluorescence was measured using a Gemini EM microplate spectrofluorometer and normalized by the vehicle-treated control enzyme activities. Vehicle-treated control corresponds to 0 on the chart. Results are presented as a mean ± SD of at least three independent experiments.

    Article Snippet: Subsequently, the HDAC assay developing solution was added and after 15 min of incubation at room temperature, the fluorescence was measured using a Gemini EM microplate spectrofluorometer (Molecular Devices, Belgium) with excitation at 360 nm and emission at 460 nm.

    Techniques: Inhibition, Activity Assay, Incubation, Fluorescence

    Pxn-KD fails to increase tyrosine phosphorylation and calcium mobilization. (A) Washed platelets obtained from control and Pxn-KD experiments were stimulated with 150 ng/mL convulxin for the indicated times. The cell lysates were resolved by SDS-polyacrylamide gel electrophoresis and then immunoblotted with an anti-phosphotyrosine mAb (4G10). The data shown are representative of three independent experiments. (B) Control and Pxn-KD platelets were labeled with GFP-Certified™ FluoForte™ dye. Changes in intracellular calcium levels after stimulation with an indicated concentration of AYPGKF or convulxin were then measured every 30 s. Data are expressed as the relative fluorescence unit (RFU) measured using a microplate spectrofluorometer (excitation, 530 nm; emission, 570 nm). The peak calcium concentration was measured after stimulation (open bars: control platelets; black bars: Pxn-KD platelets). Columns and error bars represent the mean ± s.d. ( n = 5–8). Statistical significance was determined by the Student’s t -test. (C) Control and Pxn-KD platelets were pretreated with 1 mmol/L EDTA and/or 20 μmol/L BAPTA-AM for 10 min, and then stimulated with or without 1 mmol/L AYPGKF or 150 ng/mL convulxin. P-selectin expression on GFP-positive platelets was determined by flow cytometry. Columns and error bars represent the mean ± s.d. of P-selectin expression (n = 4).

    Journal: Thrombosis Journal

    Article Title: Paxillin is an intrinsic negative regulator of platelet activation in mice

    doi: 10.1186/1477-9560-12-1

    Figure Lengend Snippet: Pxn-KD fails to increase tyrosine phosphorylation and calcium mobilization. (A) Washed platelets obtained from control and Pxn-KD experiments were stimulated with 150 ng/mL convulxin for the indicated times. The cell lysates were resolved by SDS-polyacrylamide gel electrophoresis and then immunoblotted with an anti-phosphotyrosine mAb (4G10). The data shown are representative of three independent experiments. (B) Control and Pxn-KD platelets were labeled with GFP-Certified™ FluoForte™ dye. Changes in intracellular calcium levels after stimulation with an indicated concentration of AYPGKF or convulxin were then measured every 30 s. Data are expressed as the relative fluorescence unit (RFU) measured using a microplate spectrofluorometer (excitation, 530 nm; emission, 570 nm). The peak calcium concentration was measured after stimulation (open bars: control platelets; black bars: Pxn-KD platelets). Columns and error bars represent the mean ± s.d. ( n = 5–8). Statistical significance was determined by the Student’s t -test. (C) Control and Pxn-KD platelets were pretreated with 1 mmol/L EDTA and/or 20 μmol/L BAPTA-AM for 10 min, and then stimulated with or without 1 mmol/L AYPGKF or 150 ng/mL convulxin. P-selectin expression on GFP-positive platelets was determined by flow cytometry. Columns and error bars represent the mean ± s.d. of P-selectin expression (n = 4).

    Article Snippet: After stimulation, the intracellular calcium concentration was determined by monitoring the fluorescence (excitation, 530 nm; emission, 570 nm) using a microplate spectrofluorometer (Gemini EM; Molecular Devices, Sunnyvale, CA).

    Techniques: Polyacrylamide Gel Electrophoresis, Labeling, Concentration Assay, Fluorescence, Expressing, Flow Cytometry, Cytometry

    Validation of the method with the three known genotypes of the ALDH2 gene (C/C, C/T, T/T). After the SBE reaction, the fluorescence emission spectra (580–700 nm) were measured with a microplate fluorescence reader. The NTC spectrum was subtracted from all spectra.

    Journal: Nucleic Acids Research

    Article Title: A FRET-based analysis of SNPs without fluorescent probes

    doi: 10.1093/nar/gnh155

    Figure Lengend Snippet: Validation of the method with the three known genotypes of the ALDH2 gene (C/C, C/T, T/T). After the SBE reaction, the fluorescence emission spectra (580–700 nm) were measured with a microplate fluorescence reader. The NTC spectrum was subtracted from all spectra.

    Article Snippet: The fluorescence intensity after the SBE reaction was measured with a Spectra Max Gemini Microplate Spectrofluorometer (Molecular Devices) using an excitation wavelength of 495 nm at room temperature.

    Techniques: Fluorescence