lps binding protein  (Hycult Biotech)

Journal: Transplant International

Article Title: Activation of the Innate Immune System in Brain-Dead Donors Can Be Reduced by Luminal Intestinal Preservation During Organ Procurement Surgery - A Porcine Model

doi: 10.3389/ti.2024.13569

Figure Lengend Snippet: Procurement surgery-induced release of lipopolysaccharide binding protein in plasma was reduced by intestinal PEG intervention. Procurement surgery led to a significant increase in lipopolysaccharide binding protein (LBP) in all groups except the PEG intervention group (A) . At the end of organ procurement, lipopolysaccharide binding protein was significantly lower in the brain dead + PEG-group compared to all other groups (B) . Control: control group, Brain dead: brain dead without luminal intestinal intervention, Brain dead + PEG: brain dead with luminal intestinal intervention using polyethylene glycol, Brain dead + UW: brain dead with luminal intestinal intervention using University of Wisconsin solution, LBP: Lipopolysachharide binding protein. Values presented as mean ± 95% Confidence Interval. Mixed model. * p < 0.05.

Article Snippet: EDTA Plasma samples were analyzed for lipopolysaccharide binding protein (LBP) (Hycult Biotech, HK503, Uden, Netherlands), IL-8 (Merck & Co., PCYTMAG-23K, Rahway, NJ, United States), and TNF (R&D Systems, PTA00, Minneapolis, MN, United States) according to the manufacturer’s instructions.

Techniques: Binding Assay, Control

Journal: Aging Cell

Article Title: Aging amplifies a gut microbiota immunogenic signature linked to heightened inflammation

doi: 10.1111/acel.14190

Figure Lengend Snippet: Gut microbiota composition and inflammatory status of young and aged mice. (a) β‐diversity analysis: principal coordinate analysis plot using the Bray–Curtis index from young (blue spheres) and aged (red spheres) mice. Dotted line ellipses correspond to clusters in each group. (b) α‐diversity analysis: index Chao‐1 represents community richness. (c) Heat tree plotting of colonic taxa abundance (aged vs. young) to allow quantitative visualization of community diversity data comparing the two age groups. Microbiome data (Panels A, B, and C) correspond to n = 5 cohorts with n = 15–19 per group. (d) Distal colon gene expression in young and aged mice, analyzed via qPCR ( n = 4–6 per group). (e) Distal colon gene expression in young and aged mice analyzed via Fluidigm and depicted with a heat map. Blue indicates downregulation and red indicates upregulation compared to young mice. Each square represents the mean gene expression per group. Predominantly affected genes by age are represented in individual graphs on the right side of the heat map. Data correspond to n = 5 cohorts with n = 8–15 per group. (f) Lipopolysaccharide‐binding protein (LBP) in serum and calprotectin in proximal colon contents, both analyzed by ELISA kits according to manufacturer's recommendations. Values were expressed as Z‐score considering young mice as control. Data correspond to n = 3 cohorts with n = 9–18 per group. (g) Top 25 genera correlated with LBP and calprotectin expression (Spearman rank correlation). Data correspond to n = 3 cohorts with n = 8–17 per group.

Article Snippet: Commercially available ELISA kits (Hycult Biotec, Uden, Netherlands) were utilized according to manufacturers' instructions to assess LPS‐binding protein (LBP) and calprotectin concentrations in serum and colon contents, respectively.

Techniques: Expressing, Binding Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: The Journal of Veterinary Medical Science

Article Title: Effects of pellet starch levels in automatic milking systems on rumen fermentation, plasma metabolites, and milk production in mid-lactation cows

doi: 10.1292/jvms.23-0498

Figure Lengend Snippet: Effects of feeding control or experimental pellets to cows in automatic milking systems (AMS) on plasma lipopolysaccharide-binding protein (LBP), haptoglobin (Hp), and serum amyloid A (SAA). Cows fed control pellets (control group) and experimental pellets (experimental group) in the AMS are represented by (□) and (■), respectively. The control pellet contained 30.0% starch (dry matter [DM] basis) and the experimental pellet contained 23.5% of starch (DM basis). Data collected on days 1 and 8 were averaged for each sample collection period. Data are expressed as least squares means and standard error of the mean of 12 cows per treatment.

Article Snippet: Plasma concentrations of lipopolysaccharide-binding protein (LBP) were measured using a commercially available kit (HK503; Hycult Biotechnology, Uden, the Netherlands).

Techniques: Control, Binding Assay, Starch