bloxall (Vector Laboratories)

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BLOXALL Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution

Description:

BLOXALL Endogenous Blocking Solution HRP and AP Blocking inhibits endogenous peroxidase pseudoperoxidase and alkaline phosphatase activitiesReady to use in convenient dropper bottleSimple one step protocolcompatible with formalin fixed paraffin embedded tissue sections frozen tissue sections and cell preparationsMore effective than conventional blocking methodsTissues can contain endogenous peroxidase pseudoperoxidase and or alkaline phosphatase activity that will produce background staining if an alkaline phosphatase and or peroxidase detection system and corresponding substrates are used BLOXALL Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution is a dual enzyme blocking reagent that inactivates endogenous peroxidase pseudoperoxidase and alkaline phosphatase in formalin fixed paraffin embedded tissue sections frozen tissue sections and cell preparations in a single 10 minute incubation step This reagent is provided as a ready to use reagent in a convenient dropper bottle

Catalog Number:

sp-6000

Price:

None

Category:

Blocking agents

Size:

100 ml

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Vector Laboratories bloxall

BLOXALL Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution

bloxall - by Bioz Stars, 2021-03

99/100 stars

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Activity Assay:

Article Title: Regulation of Androgen Receptor and Prostate Cancer Growth by Cyclin-dependent Kinase 5 *
Article Snippet: .. Endogenous peroxidase activity was blocked by 3% hydrogen peroxide for 10 min followed by a 1-h incubation with blocking serum (Vectastain ABC Kit, Vector Laboratories). .. The slides were then incubated for 4 h at room temperature followed by incubation with biotinylated antibody (Vectastain ABC Kit, Vector Laboratories) for 30 min.

Article Title: Analysis of leukocyte transepithelial migration using an in vivo murine colonic loop model
Article Snippet: After deparaffinization in xylene and hydration with alcohol solutions, antigen retrieval was performed with Antigen Unmasking Solution (1:100, Vector laboratories, catalog H-3301). .. Tissue was permeabilized with 0.5% Triton X-100 (MilliporeSigma) for 10 minutes at room temperature (RT), and endogenous peroxidase activity was abolished with Bloxall solution (Vector laboratories, catalog SP-6000) for 10 minutes at RT. .. Then, tissue was incubated for 60 minutes with anti–mouse Ig reagent (M.O.M., Vector Laboratories, MPX-2404).

Incubation:

Article Title: Switching-Off Adora2b in Vascular Smooth Muscle Cells Halts the Development of Pulmonary Hypertension
Article Snippet: Immunohistochemistry was performed on 5 μm sections cut from formalin-fixed, paraffin-embedded lungs. .. Sections were rehydrated through graded ethanol to water, antigen retrieval was performed using a solution of 10 mM citric acid and heated for 2 min for 3 intervals at high power using a microwave, endogenous peroxidase and alkaline phosphatase were inactivated using BLOXALL (Vector Labs, Burlingame, CA) and 2.5% normal horse serum (Vector Labs) was used as a blocking solution prior to incubation with the primary antibody. .. Following overnight incubation with the primary antibody, sections were treated with the ImmPRESS polymer detection kits for alkaline phosphatase or horse-radish peroxidase (Vector Labs) based on the host of the primary antibody and development method.

Blocking Assay:

Article Title: Cardiovascular Consequences of Prostanoid I Receptor Deletion in Microsomal Prostaglandin E Synthase-1 Deficient Hyperlipidemic Mice
Article Snippet: Mouse hearts were embedded in OCT, and 10 μm serial sections of the aortic root were cut and mounted on Superfrost Plus slides (Fisher Scientific) for analysis of lesion morphology. .. Prior to treatment with the first antibody, sections were consecutively treated to block endogenous peroxidase (3% H2 O2 for 15 min), with 10% normal serum blocking solution (dependent on host of secondary antibody, in 1%BSA/PBS for 15 min) and for endogenous biotin (streptavidin-biotin blocking kit, #SP-2002, Vector Laboratories). .. Samples were individually stained for collagen type-I (1 μg/ml, #1310-01, Southern Biotech), α-SMA (12.3 μg/ml, #F3777, Sigma), VCAM-1 (5 μg/ml, #553331, BD Bioscience) and CD11b (2.5 μg/ml, #557395, BD Bioscience), all with isotype-matched controls.

Article Title: Epilysin (MMP-28) Restrains Early Macrophage Recruitment in Pseudomonas aeruginosa Pneumonia
Article Snippet: .. Antigen retrieval was performed using antigen unmasking solution (Vector Labs, Burlingame CA) heated to 90°C for 30 min. Endogenous peroxidase was blocked using 3% hydrogen peroxide for 10 min. Avidin and Biotin block (Vector Labs) was applied to each section for 10 min. .. After each incubation, the sections were washed three times in PBS.

bloxall (Vector Laboratories)

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