hrp conjugated streptavidin  (Vector Laboratories)


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    Vector Laboratories hrp conjugated streptavidin
    Hrp Conjugated Streptavidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hrp conjugated streptavidin  (Vector Laboratories)


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    Vector Laboratories hrp conjugated streptavidin
    Hrp Conjugated Streptavidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated streptavidin/product/Vector Laboratories
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    horseradish peroxidase conjugated streptavidin  (Vector Laboratories)


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    Vector Laboratories horseradish peroxidase conjugated streptavidin
    Horseradish Peroxidase Conjugated Streptavidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pro spc  (Vector Laboratories)


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    Vector Laboratories pro spc
    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear <t>lamin</t> <t>b1</t> with <t>pro-SPC</t> (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.
    Pro Spc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Timing and cell specificity of senescence drives postnatal lung development and injury"

    Article Title: Timing and cell specificity of senescence drives postnatal lung development and injury

    Journal: Nature Communications

    doi: 10.1038/s41467-023-35985-4

    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear lamin b1 with pro-SPC (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.
    Figure Legend Snippet: C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear lamin b1 with pro-SPC (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.

    Techniques Used: Immunofluorescence

    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. Senescent type II cells were isolated from these mice through the sorting of pro-SPC and C12FDG positive cells. a Immunofluorescence of pro-SPC was performed in these cells after 24 h of culture. Arrows denote pro-SPC positive cells. Bar size: 50 µm. b Luminex assay was performed to evaluate the levels of SASP factors in the supernatants. c , d C57BL/6 J neonatal mice were intranasally injected with 20 μl of culture medium with or without dilution (1-part medium:1-part saline) of cultured cells at pnd7 and pnd10. H&E and vWF staining was performed to measure mean linear intercept (Lm), radial alveolar count (RAC) and number of blood vessels in the lung at pnd14. Bar size: 100 µm. Data are expressed as mean ± SEM. N = 5–6 mice per group. Source data are provided as a Source Data file. One-way ANOVA followed by Tukey post-test was used for multiple comparisons ( c , d ), while t -test was used in panel ( b ). * P < 0.05, ** P < 0.01, *** P < 0.001 vs air ( b ) or vehicle ( c , d ).
    Figure Legend Snippet: C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. Senescent type II cells were isolated from these mice through the sorting of pro-SPC and C12FDG positive cells. a Immunofluorescence of pro-SPC was performed in these cells after 24 h of culture. Arrows denote pro-SPC positive cells. Bar size: 50 µm. b Luminex assay was performed to evaluate the levels of SASP factors in the supernatants. c , d C57BL/6 J neonatal mice were intranasally injected with 20 μl of culture medium with or without dilution (1-part medium:1-part saline) of cultured cells at pnd7 and pnd10. H&E and vWF staining was performed to measure mean linear intercept (Lm), radial alveolar count (RAC) and number of blood vessels in the lung at pnd14. Bar size: 100 µm. Data are expressed as mean ± SEM. N = 5–6 mice per group. Source data are provided as a Source Data file. One-way ANOVA followed by Tukey post-test was used for multiple comparisons ( c , d ), while t -test was used in panel ( b ). * P < 0.05, ** P < 0.01, *** P < 0.001 vs air ( b ) or vehicle ( c , d ).

    Techniques Used: Isolation, Immunofluorescence, Luminex, Injection, Cell Culture, Staining

    streptavidin peroxidase steps  (Vector Laboratories)


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    Vector Laboratories streptavidin peroxidase steps
    Streptavidin Peroxidase Steps, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti streptavidin antibody  (Vector Laboratories)


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    Vector Laboratories anti streptavidin antibody
    Anti Streptavidin Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti streptavidin antibody  (Vector Laboratories)


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    Vector Laboratories anti streptavidin antibody
    Anti Streptavidin Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    operation sp kit  (Vector Laboratories)


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    Vector Laboratories operation sp kit
    Operation Sp Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    peroxidase conjugated streptavidin  (Vector Laboratories)


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    Vector Laboratories peroxidase conjugated streptavidin
    Peroxidase Conjugated Streptavidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti species antibody  (Vector Laboratories)


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    Vector Laboratories anti species antibody
    Anti Species Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    horseradish peroxidase conjugated streptavidin  (Vector Laboratories)


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    Vector Laboratories horseradish peroxidase conjugated streptavidin
    Horseradish Peroxidase Conjugated Streptavidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories hrp conjugated streptavidin
    Hrp Conjugated Streptavidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories pro spc
    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear <t>lamin</t> <t>b1</t> with <t>pro-SPC</t> (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.
    Pro Spc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories streptavidin peroxidase steps
    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear <t>lamin</t> <t>b1</t> with <t>pro-SPC</t> (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.
    Streptavidin Peroxidase Steps, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories anti streptavidin antibody
    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear <t>lamin</t> <t>b1</t> with <t>pro-SPC</t> (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.
    Anti Streptavidin Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories operation sp kit
    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear <t>lamin</t> <t>b1</t> with <t>pro-SPC</t> (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.
    Operation Sp Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories peroxidase conjugated streptavidin
    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear <t>lamin</t> <t>b1</t> with <t>pro-SPC</t> (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.
    Peroxidase Conjugated Streptavidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories anti species antibody
    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear <t>lamin</t> <t>b1</t> with <t>pro-SPC</t> (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.
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    Image Search Results


    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear lamin b1 with pro-SPC (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.

    Journal: Nature Communications

    Article Title: Timing and cell specificity of senescence drives postnatal lung development and injury

    doi: 10.1038/s41467-023-35985-4

    Figure Lengend Snippet: C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear lamin b1 with pro-SPC (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.

    Article Snippet: Sections were de-paraffinized and subjected to heat-mediated antigen retrieval in a citrate buffer solution (Vector Labs), then incubated with antibodies against lamin b1, p21, Ki67, γ-H2AX, 53BP1, 8-oxo-DG, phosphor-p53, Hopx, vWF, vimentin, pro-SPC, Pdgfra, Sox9, and F4/80 overnight at 4 °C.

    Techniques: Immunofluorescence

    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. Senescent type II cells were isolated from these mice through the sorting of pro-SPC and C12FDG positive cells. a Immunofluorescence of pro-SPC was performed in these cells after 24 h of culture. Arrows denote pro-SPC positive cells. Bar size: 50 µm. b Luminex assay was performed to evaluate the levels of SASP factors in the supernatants. c , d C57BL/6 J neonatal mice were intranasally injected with 20 μl of culture medium with or without dilution (1-part medium:1-part saline) of cultured cells at pnd7 and pnd10. H&E and vWF staining was performed to measure mean linear intercept (Lm), radial alveolar count (RAC) and number of blood vessels in the lung at pnd14. Bar size: 100 µm. Data are expressed as mean ± SEM. N = 5–6 mice per group. Source data are provided as a Source Data file. One-way ANOVA followed by Tukey post-test was used for multiple comparisons ( c , d ), while t -test was used in panel ( b ). * P < 0.05, ** P < 0.01, *** P < 0.001 vs air ( b ) or vehicle ( c , d ).

    Journal: Nature Communications

    Article Title: Timing and cell specificity of senescence drives postnatal lung development and injury

    doi: 10.1038/s41467-023-35985-4

    Figure Lengend Snippet: C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. Senescent type II cells were isolated from these mice through the sorting of pro-SPC and C12FDG positive cells. a Immunofluorescence of pro-SPC was performed in these cells after 24 h of culture. Arrows denote pro-SPC positive cells. Bar size: 50 µm. b Luminex assay was performed to evaluate the levels of SASP factors in the supernatants. c , d C57BL/6 J neonatal mice were intranasally injected with 20 μl of culture medium with or without dilution (1-part medium:1-part saline) of cultured cells at pnd7 and pnd10. H&E and vWF staining was performed to measure mean linear intercept (Lm), radial alveolar count (RAC) and number of blood vessels in the lung at pnd14. Bar size: 100 µm. Data are expressed as mean ± SEM. N = 5–6 mice per group. Source data are provided as a Source Data file. One-way ANOVA followed by Tukey post-test was used for multiple comparisons ( c , d ), while t -test was used in panel ( b ). * P < 0.05, ** P < 0.01, *** P < 0.001 vs air ( b ) or vehicle ( c , d ).

    Article Snippet: Sections were de-paraffinized and subjected to heat-mediated antigen retrieval in a citrate buffer solution (Vector Labs), then incubated with antibodies against lamin b1, p21, Ki67, γ-H2AX, 53BP1, 8-oxo-DG, phosphor-p53, Hopx, vWF, vimentin, pro-SPC, Pdgfra, Sox9, and F4/80 overnight at 4 °C.

    Techniques: Isolation, Immunofluorescence, Luminex, Injection, Cell Culture, Staining