streptavidin (Vector Laboratories)


Name:
Streptavidin Biotin Blocking Kit
Description:
Streptavidin Biotin Blocking Kit blocks all endogenous biotin biotin receptors and streptavidin binding sites present in tissues This kit is designed for use with streptavidin detection systems such as the VECTASTAIN Universal Quick kits if streptavidin or biotinylated reagents bind non specifically to tissues or proteins This blocking kit consists of 18 ml of streptavidin and 18 ml of biotin in convenient dropper bottles
Catalog Number:
sp-2002
Price:
None
Category:
Blocking agents
Size:
1 kit
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Structured Review

Streptavidin Biotin Blocking Kit blocks all endogenous biotin biotin receptors and streptavidin binding sites present in tissues This kit is designed for use with streptavidin detection systems such as the VECTASTAIN Universal Quick kits if streptavidin or biotinylated reagents bind non specifically to tissues or proteins This blocking kit consists of 18 ml of streptavidin and 18 ml of biotin in convenient dropper bottles
https://www.bioz.com/result/streptavidin/product/Vector Laboratories
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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Blocking Assay:Article Title: Antigen Display, T-Cell Activation, and Immune Evasion during Acute and Chronic Ehrlichiosis Article Snippet: For the detection of E. muris , biotinylated Ec18.1 (1 h at RT) ( ) and streptavidin-Alexafluor-488 (30 min; Invitrogen, Carlsbad, CA) were used. .. When biotinylated antibodies were used, a Article Title: Loss of α2-6 sialylation promotes the transformation of synovial fibroblasts into a pro-inflammatory phenotype in Rheumatoid Arthritis Article Snippet: .. Endogenous biotin was blocked with the Article Title: Epithelial-to-Mesenchymal Transition of RPE Cells In Vitro Confers Increased β1,6-N-Glycosylation and Increased Susceptibility to Galectin-3 Binding Article Snippet: In order to remove the paraffin and to rehydrate the sections, they were first rinsed with xylene (once for 5 min) and isopropanol (twice for 5 min), followed by ethanol (96% and 70%—each for 5 min) and finally millipore water (5 min). .. Prior to incubation with plant lectins, sections were blocked for 45 minutes in 1x carbohydrate-free blocking solution (Vector laboratories) and streptavidin blocking solution ( Article Title: Antigen Display, T-Cell Activation, and Immune Evasion during Acute and Chronic Ehrlichiosis Article Snippet: The saponin-permeabilized cells were incubated with biotinylated Ec18.1 for 1 h at RT, followed by incubation with streptavidin-Alexafluor-488 (Invitrogen) for 30 min. .. Since all of the antibodies used in this staining procedure were biotinylated, a Article Title: Loss of Mammal-specific Tectorial Membrane Component Carcinoembryonic Antigen Cell Adhesion Molecule 16 (CEACAM16) Leads to Hearing Impairment at Low and High Frequencies * Article Snippet: .. Antigen retrieval was achieved by heating to ∼95 °C of the tissue sections in Target Retrieval Solution, pH 9 (DakoCytomation), for 30 min followed by cooling to room temperature for 20 min. After blockage of endogenous peroxidase and biotin by incubation with 3% H2 O2 , 10% methanol in PBS for 5 min at room temperature and by using the Article Title: Quantifying the Biomechanics of Conception: L-Selectin-Mediated Blastocyst Implantation Mechanics with Engineered “Trophospheres” Article Snippet: Flow cytometry primary antibodies were anti-human L-selectin (CD62L) monoclonal (Clone #4G8) antibody BBA24 (R & D Systems, Minneapolis, MN) or isotype-matched control (clone MOPC-1; BD Biosciences, San Jose, CA), and the secondary antibody was Alexa Fluor 488 goat anti-mouse IgG1 (Invitrogen, Carlsbad, CA). .. Immunoreagents for immunohistochemistry were mouse IgG1 (clone MOPC-21; BD Pharmingen), L-selectin antibody (Clone #4G8; R & D Systems), biotinylated goat anti-mouse IgG (H+L) (Vector Laboratories, Burlingame, CA), Article Title: Osteoarticular tissue infection and development of skeletal pathology in murine brucellosis Article Snippet: Nonspecific binding was blocked for 1 hour at room temperature using 2% BSA (Sigma), 2% normal non-immune donkey serum (Vector Labs), and 0.05% Tween-20 in 1×PBS. .. Sections were rinsed briefly with 1×PBS and additionally blocked using Article Title: Regulation of Expression of Microvillus Membrane Proteins by Estrogen in Baboon Fetal Ovarian Oocytes 1 Article Snippet: Briefly, for detection with DAB, sections were treated with VectaStain Elite Kit (Vector Laboratories Inc., Burlingame, CA), rinsed, stained with DAB-imidazole-H2 O2 , counterstained with Harris hematoxylin, mounted in Cytoseal-XYL (Richard Allan Scientific, Kalamazoo, MI), and examined by light microscopy [ ]. .. For immunofluorescence analysis, before blocking with NHS or NGS, sections were incubated with Image-iT signal enhancer (Molecular Probes) and blocked for endogenous biotin with the Incubation:Article Title: Epithelial-to-Mesenchymal Transition of RPE Cells In Vitro Confers Increased β1,6-N-Glycosylation and Increased Susceptibility to Galectin-3 Binding Article Snippet: In order to remove the paraffin and to rehydrate the sections, they were first rinsed with xylene (once for 5 min) and isopropanol (twice for 5 min), followed by ethanol (96% and 70%—each for 5 min) and finally millipore water (5 min). .. Prior to incubation with plant lectins, sections were blocked for 45 minutes in 1x carbohydrate-free blocking solution (Vector laboratories) and streptavidin blocking solution ( Article Title: Loss of Mammal-specific Tectorial Membrane Component Carcinoembryonic Antigen Cell Adhesion Molecule 16 (CEACAM16) Leads to Hearing Impairment at Low and High Frequencies * Article Snippet: .. Antigen retrieval was achieved by heating to ∼95 °C of the tissue sections in Target Retrieval Solution, pH 9 (DakoCytomation), for 30 min followed by cooling to room temperature for 20 min. After blockage of endogenous peroxidase and biotin by incubation with 3% H2 O2 , 10% methanol in PBS for 5 min at room temperature and by using the Article Title: Regulation of Expression of Microvillus Membrane Proteins by Estrogen in Baboon Fetal Ovarian Oocytes 1 Article Snippet: Briefly, for detection with DAB, sections were treated with VectaStain Elite Kit (Vector Laboratories Inc., Burlingame, CA), rinsed, stained with DAB-imidazole-H2 O2 , counterstained with Harris hematoxylin, mounted in Cytoseal-XYL (Richard Allan Scientific, Kalamazoo, MI), and examined by light microscopy [ ]. .. For immunofluorescence analysis, before blocking with NHS or NGS, sections were incubated with Image-iT signal enhancer (Molecular Probes) and blocked for endogenous biotin with the Staining:Article Title: Epithelial-to-Mesenchymal Transition of RPE Cells In Vitro Confers Increased β1,6-N-Glycosylation and Increased Susceptibility to Galectin-3 Binding Article Snippet: In order to remove the paraffin and to rehydrate the sections, they were first rinsed with xylene (once for 5 min) and isopropanol (twice for 5 min), followed by ethanol (96% and 70%—each for 5 min) and finally millipore water (5 min). .. Prior to incubation with plant lectins, sections were blocked for 45 minutes in 1x carbohydrate-free blocking solution (Vector laboratories) and streptavidin blocking solution ( Article Title: Antigen Display, T-Cell Activation, and Immune Evasion during Acute and Chronic Ehrlichiosis Article Snippet: The saponin-permeabilized cells were incubated with biotinylated Ec18.1 for 1 h at RT, followed by incubation with streptavidin-Alexafluor-488 (Invitrogen) for 30 min. .. Since all of the antibodies used in this staining procedure were biotinylated, a Article Title: Loss of Mammal-specific Tectorial Membrane Component Carcinoembryonic Antigen Cell Adhesion Molecule 16 (CEACAM16) Leads to Hearing Impairment at Low and High Frequencies * Article Snippet: .. Antigen retrieval was achieved by heating to ∼95 °C of the tissue sections in Target Retrieval Solution, pH 9 (DakoCytomation), for 30 min followed by cooling to room temperature for 20 min. After blockage of endogenous peroxidase and biotin by incubation with 3% H2 O2 , 10% methanol in PBS for 5 min at room temperature and by using the Immunohistochemistry:Article Title: Quantifying the Biomechanics of Conception: L-Selectin-Mediated Blastocyst Implantation Mechanics with Engineered “Trophospheres” Article Snippet: Flow cytometry primary antibodies were anti-human L-selectin (CD62L) monoclonal (Clone #4G8) antibody BBA24 (R & D Systems, Minneapolis, MN) or isotype-matched control (clone MOPC-1; BD Biosciences, San Jose, CA), and the secondary antibody was Alexa Fluor 488 goat anti-mouse IgG1 (Invitrogen, Carlsbad, CA). .. Immunoreagents for immunohistochemistry were mouse IgG1 (clone MOPC-21; BD Pharmingen), L-selectin antibody (Clone #4G8; R & D Systems), biotinylated goat anti-mouse IgG (H+L) (Vector Laboratories, Burlingame, CA), Immunofluorescence:Article Title: Regulation of Expression of Microvillus Membrane Proteins by Estrogen in Baboon Fetal Ovarian Oocytes 1 Article Snippet: Briefly, for detection with DAB, sections were treated with VectaStain Elite Kit (Vector Laboratories Inc., Burlingame, CA), rinsed, stained with DAB-imidazole-H2 O2 , counterstained with Harris hematoxylin, mounted in Cytoseal-XYL (Richard Allan Scientific, Kalamazoo, MI), and examined by light microscopy [ ]. .. For immunofluorescence analysis, before blocking with NHS or NGS, sections were incubated with Image-iT signal enhancer (Molecular Probes) and blocked for endogenous biotin with the Next-Generation Sequencing:Article Title: Regulation of Expression of Microvillus Membrane Proteins by Estrogen in Baboon Fetal Ovarian Oocytes 1 Article Snippet: Briefly, for detection with DAB, sections were treated with VectaStain Elite Kit (Vector Laboratories Inc., Burlingame, CA), rinsed, stained with DAB-imidazole-H2 O2 , counterstained with Harris hematoxylin, mounted in Cytoseal-XYL (Richard Allan Scientific, Kalamazoo, MI), and examined by light microscopy [ ]. .. For immunofluorescence analysis, before blocking with NHS or NGS, sections were incubated with Image-iT signal enhancer (Molecular Probes) and blocked for endogenous biotin with the |