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    Structured Review

    Vector Laboratories pbs
    IFN-γ expression and CD8 T cells drive LN expansion. (a and b) Efficacy of anti-Ly6G antibody-mediated neutrophil depletion ( n = 3–6 mice from two independent experiments; a) or anti-NK1.1 antibody-mediated NK cell depletion ( n = 5 mice from two independent experiments; b); unpaired Student’s t test. (c) Counts of lymphocytes and neutrophils in popLNs in diphtheria toxin–treated Clec9aCre:Rosa-iDTR or iDTR mice 2 d after unilateral sciatic denervation; n = 3–4 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (d) Efficacy of diphtheria toxin–mediated Clec9aCre:Rosa-iDTR –specific cell depletion. Counts of total cells and DC subsets in spleen in diphtheria toxin–treated Clec9aCre:Rosa-iDTR or control mice 2 d after unilateral sciatic denervation; n = 3–4 mice; data are representative of two independent experiments; one-way ANOVA; Tukey’s post-test. (e) Counts of indicated subsets in popLNs 1 wk after unilateral sciatic denervation with or without IFN-γ blockade; n = 5 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (f) Percentage of IFN-γ + cells in indicated subsets; n = 5 mice; data are representative of two independent experiments; unpaired Student’s t test. (g) Counts of indicated subsets in popLNs 1 wk after unilateral sciatic denervation with or without CD8 depletion; n = 5 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (h) Percentage of IFN-γ + cells in indicated subsets from popLNs 1 wk after unilateral sciatic denervation with <t>PBS</t> or clenbuterol treatment; n = 5 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (i) Presence of sympathetic TH + nerves close to CD8 T cells in the popLN. B cell follicles are highlighted with dotted lines. Scale bar, 50 µm. (j) Percentage of viable isolated CD8 T cells after in vitro restimulation with PMA/ionomycin (P/I) in the presence or absence of clenbuterol; n = 7 mice from two independent experiments. Clec9aCre:Rosa-iDTR and iDTR mice were 8–12 wk old and bred in the animal facility. The other mice in this figure were 8–12-wk-old WT males purchased from Charles River or Janvier Labs. All data are presented as mean ± SEM; *, P
    Pbs, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs/product/Vector Laboratories
    Average 97 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pbs - by Bioz Stars, 2022-12
    97/100 stars

    Images

    1) Product Images from "Loss of direct adrenergic innervation after peripheral nerve injury causes lymph node expansion through IFN-γ"

    Article Title: Loss of direct adrenergic innervation after peripheral nerve injury causes lymph node expansion through IFN-γ

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20202377

    IFN-γ expression and CD8 T cells drive LN expansion. (a and b) Efficacy of anti-Ly6G antibody-mediated neutrophil depletion ( n = 3–6 mice from two independent experiments; a) or anti-NK1.1 antibody-mediated NK cell depletion ( n = 5 mice from two independent experiments; b); unpaired Student’s t test. (c) Counts of lymphocytes and neutrophils in popLNs in diphtheria toxin–treated Clec9aCre:Rosa-iDTR or iDTR mice 2 d after unilateral sciatic denervation; n = 3–4 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (d) Efficacy of diphtheria toxin–mediated Clec9aCre:Rosa-iDTR –specific cell depletion. Counts of total cells and DC subsets in spleen in diphtheria toxin–treated Clec9aCre:Rosa-iDTR or control mice 2 d after unilateral sciatic denervation; n = 3–4 mice; data are representative of two independent experiments; one-way ANOVA; Tukey’s post-test. (e) Counts of indicated subsets in popLNs 1 wk after unilateral sciatic denervation with or without IFN-γ blockade; n = 5 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (f) Percentage of IFN-γ + cells in indicated subsets; n = 5 mice; data are representative of two independent experiments; unpaired Student’s t test. (g) Counts of indicated subsets in popLNs 1 wk after unilateral sciatic denervation with or without CD8 depletion; n = 5 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (h) Percentage of IFN-γ + cells in indicated subsets from popLNs 1 wk after unilateral sciatic denervation with PBS or clenbuterol treatment; n = 5 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (i) Presence of sympathetic TH + nerves close to CD8 T cells in the popLN. B cell follicles are highlighted with dotted lines. Scale bar, 50 µm. (j) Percentage of viable isolated CD8 T cells after in vitro restimulation with PMA/ionomycin (P/I) in the presence or absence of clenbuterol; n = 7 mice from two independent experiments. Clec9aCre:Rosa-iDTR and iDTR mice were 8–12 wk old and bred in the animal facility. The other mice in this figure were 8–12-wk-old WT males purchased from Charles River or Janvier Labs. All data are presented as mean ± SEM; *, P
    Figure Legend Snippet: IFN-γ expression and CD8 T cells drive LN expansion. (a and b) Efficacy of anti-Ly6G antibody-mediated neutrophil depletion ( n = 3–6 mice from two independent experiments; a) or anti-NK1.1 antibody-mediated NK cell depletion ( n = 5 mice from two independent experiments; b); unpaired Student’s t test. (c) Counts of lymphocytes and neutrophils in popLNs in diphtheria toxin–treated Clec9aCre:Rosa-iDTR or iDTR mice 2 d after unilateral sciatic denervation; n = 3–4 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (d) Efficacy of diphtheria toxin–mediated Clec9aCre:Rosa-iDTR –specific cell depletion. Counts of total cells and DC subsets in spleen in diphtheria toxin–treated Clec9aCre:Rosa-iDTR or control mice 2 d after unilateral sciatic denervation; n = 3–4 mice; data are representative of two independent experiments; one-way ANOVA; Tukey’s post-test. (e) Counts of indicated subsets in popLNs 1 wk after unilateral sciatic denervation with or without IFN-γ blockade; n = 5 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (f) Percentage of IFN-γ + cells in indicated subsets; n = 5 mice; data are representative of two independent experiments; unpaired Student’s t test. (g) Counts of indicated subsets in popLNs 1 wk after unilateral sciatic denervation with or without CD8 depletion; n = 5 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (h) Percentage of IFN-γ + cells in indicated subsets from popLNs 1 wk after unilateral sciatic denervation with PBS or clenbuterol treatment; n = 5 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (i) Presence of sympathetic TH + nerves close to CD8 T cells in the popLN. B cell follicles are highlighted with dotted lines. Scale bar, 50 µm. (j) Percentage of viable isolated CD8 T cells after in vitro restimulation with PMA/ionomycin (P/I) in the presence or absence of clenbuterol; n = 7 mice from two independent experiments. Clec9aCre:Rosa-iDTR and iDTR mice were 8–12 wk old and bred in the animal facility. The other mice in this figure were 8–12-wk-old WT males purchased from Charles River or Janvier Labs. All data are presented as mean ± SEM; *, P

    Techniques Used: Expressing, Mouse Assay, Isolation, In Vitro

    Sympathetic nerves curb LN responses. (a) Identification of sympathetic (red, TH) and nociceptive sensory (green, CGRP) nerves together with neurofilament (blue, NF) in the sciatic nerve. Scale bar, 200 µm. (b) Innervation of nociceptive sensory (left) and sympathetic (right) nerves to the popLN (circled), costained with CD31. Scale bar, 200 µm. (c) Innervation of sympathetic nerves to the popLN in untreated sham conditions, after systemic 6-OHDA treatment, local phenol treatment, or femoral (Fx), sciatic (Sx) or femoral and sciatic dual denervation (FSx) surgeries. Scale bars, 50 µm. (d) Cellularity of popLNs 1 wk after unilateral sciatic denervation in combination with substance P (SP), CGRP, or combined treatment (S+C); n = 5 or 6 mice from two independent experiments; two-way ANOVA; Šídák’s post-test. (e) Cellularity of the popLN 1 wk after unilateral sciatic denervation in combination with PBS or clenbuterol treatment; n = 4 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (f) Cellularity of the popLN in Adrb2 −/− mice 1 wk after unilateral sciatic denervation in combination with PBS or clenbuterol treatment; n = 4 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. Adrb2 −/− mice were 8–12-wk-old males and bred in our animal facility. All other mice in this figure were 8–12-wk-old WT males purchased from Charles River or Janvier Labs. All data are presented as mean ± SEM; *, P
    Figure Legend Snippet: Sympathetic nerves curb LN responses. (a) Identification of sympathetic (red, TH) and nociceptive sensory (green, CGRP) nerves together with neurofilament (blue, NF) in the sciatic nerve. Scale bar, 200 µm. (b) Innervation of nociceptive sensory (left) and sympathetic (right) nerves to the popLN (circled), costained with CD31. Scale bar, 200 µm. (c) Innervation of sympathetic nerves to the popLN in untreated sham conditions, after systemic 6-OHDA treatment, local phenol treatment, or femoral (Fx), sciatic (Sx) or femoral and sciatic dual denervation (FSx) surgeries. Scale bars, 50 µm. (d) Cellularity of popLNs 1 wk after unilateral sciatic denervation in combination with substance P (SP), CGRP, or combined treatment (S+C); n = 5 or 6 mice from two independent experiments; two-way ANOVA; Šídák’s post-test. (e) Cellularity of the popLN 1 wk after unilateral sciatic denervation in combination with PBS or clenbuterol treatment; n = 4 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (f) Cellularity of the popLN in Adrb2 −/− mice 1 wk after unilateral sciatic denervation in combination with PBS or clenbuterol treatment; n = 4 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. Adrb2 −/− mice were 8–12-wk-old males and bred in our animal facility. All other mice in this figure were 8–12-wk-old WT males purchased from Charles River or Janvier Labs. All data are presented as mean ± SEM; *, P

    Techniques Used: Mouse Assay

    2) Product Images from "Loss of direct adrenergic innervation after peripheral nerve injury causes lymph node expansion through IFN-γ"

    Article Title: Loss of direct adrenergic innervation after peripheral nerve injury causes lymph node expansion through IFN-γ

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20202377

    IFN-γ expression and CD8 T cells drive LN expansion. (a and b) Efficacy of anti-Ly6G antibody-mediated neutrophil depletion ( n = 3–6 mice from two independent experiments; a) or anti-NK1.1 antibody-mediated NK cell depletion ( n = 5 mice from two independent experiments; b); unpaired Student’s t test. (c) Counts of lymphocytes and neutrophils in popLNs in diphtheria toxin–treated Clec9aCre:Rosa-iDTR or iDTR mice 2 d after unilateral sciatic denervation; n = 3–4 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (d) Efficacy of diphtheria toxin–mediated Clec9aCre:Rosa-iDTR –specific cell depletion. Counts of total cells and DC subsets in spleen in diphtheria toxin–treated Clec9aCre:Rosa-iDTR or control mice 2 d after unilateral sciatic denervation; n = 3–4 mice; data are representative of two independent experiments; one-way ANOVA; Tukey’s post-test. (e) Counts of indicated subsets in popLNs 1 wk after unilateral sciatic denervation with or without IFN-γ blockade; n = 5 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (f) Percentage of IFN-γ + cells in indicated subsets; n = 5 mice; data are representative of two independent experiments; unpaired Student’s t test. (g) Counts of indicated subsets in popLNs 1 wk after unilateral sciatic denervation with or without CD8 depletion; n = 5 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (h) Percentage of IFN-γ + cells in indicated subsets from popLNs 1 wk after unilateral sciatic denervation with PBS or clenbuterol treatment; n = 5 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (i) Presence of sympathetic TH + nerves close to CD8 T cells in the popLN. B cell follicles are highlighted with dotted lines. Scale bar, 50 µm. (j) Percentage of viable isolated CD8 T cells after in vitro restimulation with PMA/ionomycin (P/I) in the presence or absence of clenbuterol; n = 7 mice from two independent experiments. Clec9aCre:Rosa-iDTR and iDTR mice were 8–12 wk old and bred in the animal facility. The other mice in this figure were 8–12-wk-old WT males purchased from Charles River or Janvier Labs. All data are presented as mean ± SEM; *, P
    Figure Legend Snippet: IFN-γ expression and CD8 T cells drive LN expansion. (a and b) Efficacy of anti-Ly6G antibody-mediated neutrophil depletion ( n = 3–6 mice from two independent experiments; a) or anti-NK1.1 antibody-mediated NK cell depletion ( n = 5 mice from two independent experiments; b); unpaired Student’s t test. (c) Counts of lymphocytes and neutrophils in popLNs in diphtheria toxin–treated Clec9aCre:Rosa-iDTR or iDTR mice 2 d after unilateral sciatic denervation; n = 3–4 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (d) Efficacy of diphtheria toxin–mediated Clec9aCre:Rosa-iDTR –specific cell depletion. Counts of total cells and DC subsets in spleen in diphtheria toxin–treated Clec9aCre:Rosa-iDTR or control mice 2 d after unilateral sciatic denervation; n = 3–4 mice; data are representative of two independent experiments; one-way ANOVA; Tukey’s post-test. (e) Counts of indicated subsets in popLNs 1 wk after unilateral sciatic denervation with or without IFN-γ blockade; n = 5 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (f) Percentage of IFN-γ + cells in indicated subsets; n = 5 mice; data are representative of two independent experiments; unpaired Student’s t test. (g) Counts of indicated subsets in popLNs 1 wk after unilateral sciatic denervation with or without CD8 depletion; n = 5 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (h) Percentage of IFN-γ + cells in indicated subsets from popLNs 1 wk after unilateral sciatic denervation with PBS or clenbuterol treatment; n = 5 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (i) Presence of sympathetic TH + nerves close to CD8 T cells in the popLN. B cell follicles are highlighted with dotted lines. Scale bar, 50 µm. (j) Percentage of viable isolated CD8 T cells after in vitro restimulation with PMA/ionomycin (P/I) in the presence or absence of clenbuterol; n = 7 mice from two independent experiments. Clec9aCre:Rosa-iDTR and iDTR mice were 8–12 wk old and bred in the animal facility. The other mice in this figure were 8–12-wk-old WT males purchased from Charles River or Janvier Labs. All data are presented as mean ± SEM; *, P

    Techniques Used: Expressing, Mouse Assay, Isolation, In Vitro

    Sympathetic nerves curb LN responses. (a) Identification of sympathetic (red, TH) and nociceptive sensory (green, CGRP) nerves together with neurofilament (blue, NF) in the sciatic nerve. Scale bar, 200 µm. (b) Innervation of nociceptive sensory (left) and sympathetic (right) nerves to the popLN (circled), costained with CD31. Scale bar, 200 µm. (c) Innervation of sympathetic nerves to the popLN in untreated sham conditions, after systemic 6-OHDA treatment, local phenol treatment, or femoral (Fx), sciatic (Sx) or femoral and sciatic dual denervation (FSx) surgeries. Scale bars, 50 µm. (d) Cellularity of popLNs 1 wk after unilateral sciatic denervation in combination with substance P (SP), CGRP, or combined treatment (S+C); n = 5 or 6 mice from two independent experiments; two-way ANOVA; Šídák’s post-test. (e) Cellularity of the popLN 1 wk after unilateral sciatic denervation in combination with PBS or clenbuterol treatment; n = 4 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (f) Cellularity of the popLN in Adrb2 −/− mice 1 wk after unilateral sciatic denervation in combination with PBS or clenbuterol treatment; n = 4 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. Adrb2 −/− mice were 8–12-wk-old males and bred in our animal facility. All other mice in this figure were 8–12-wk-old WT males purchased from Charles River or Janvier Labs. All data are presented as mean ± SEM; *, P
    Figure Legend Snippet: Sympathetic nerves curb LN responses. (a) Identification of sympathetic (red, TH) and nociceptive sensory (green, CGRP) nerves together with neurofilament (blue, NF) in the sciatic nerve. Scale bar, 200 µm. (b) Innervation of nociceptive sensory (left) and sympathetic (right) nerves to the popLN (circled), costained with CD31. Scale bar, 200 µm. (c) Innervation of sympathetic nerves to the popLN in untreated sham conditions, after systemic 6-OHDA treatment, local phenol treatment, or femoral (Fx), sciatic (Sx) or femoral and sciatic dual denervation (FSx) surgeries. Scale bars, 50 µm. (d) Cellularity of popLNs 1 wk after unilateral sciatic denervation in combination with substance P (SP), CGRP, or combined treatment (S+C); n = 5 or 6 mice from two independent experiments; two-way ANOVA; Šídák’s post-test. (e) Cellularity of the popLN 1 wk after unilateral sciatic denervation in combination with PBS or clenbuterol treatment; n = 4 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. (f) Cellularity of the popLN in Adrb2 −/− mice 1 wk after unilateral sciatic denervation in combination with PBS or clenbuterol treatment; n = 4 mice; data are representative of two independent experiments; two-way ANOVA; Šídák’s post-test. Adrb2 −/− mice were 8–12-wk-old males and bred in our animal facility. All other mice in this figure were 8–12-wk-old WT males purchased from Charles River or Janvier Labs. All data are presented as mean ± SEM; *, P

    Techniques Used: Mouse Assay

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    Vector Laboratories streptavidin biotin blocking kit
    Streptavidin Biotin Blocking Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin biotin blocking kit/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Vector Laboratories streptavidin biotin
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    https://www.bioz.com/result/streptavidin biotin/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavidin biotin - by Bioz Stars, 2022-12
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