dinitrophenyl (Vector Laboratories)

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Anti DNP Dinitrophenyl Unconjugated

Description:

Anti DNP Dinitrophenyl Unconjugated Dinitrophenyl DNP is not found endogenously in tissues and is an excellent alternative to biotin for use in applications requiring biotin free systems Probes or antibodies labeled with DNP can be detected using alkaline phosphatase labeled DNP or using the biotinylated antibody followed by a streptavidin or avidin system

Catalog Number:

sp-0603

Price:

None

Category:

Carbohydrates or its derivatives

Size:

1 mg

Host:

Rabbit

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Structured Review

Vector Laboratories dinitrophenyl

dinitrophenyl - by Bioz Stars, 2021-03

93/100 stars

Images

1) Product Images from "PCR-generated padlock probes detect single nucleotide variation in genomic DNA"

Article Title: PCR-generated padlock probes detect single nucleotide variation in genomic DNA

Journal: Nucleic Acids Research

doi:

PCR-generated padlock probes identify individual chromosomes 13 and 21. In situ hybridization was performed with cenT and cenC padlock probes labeled with dinitrophenyl-11-dUTP and digoxigenin-11-dUTP, respectively. Probes were visualized with fluorescein-labeled anti-dinitrophenyl Fab fragment (Molecular Probes) and rhodamine-labeled anti-digoxigenin Fab fragment (Boehringer-Mannheim GmbH). The green and red signals were quantitated from 10 metaphase images, where the background was subtracted and the strongest signal was set to 100%. The mean signal intensities are shown with the standard errors of the means.

Figure Legend Snippet: PCR-generated padlock probes identify individual chromosomes 13 and 21. In situ hybridization was performed with cenT and cenC padlock probes labeled with dinitrophenyl-11-dUTP and digoxigenin-11-dUTP, respectively. Probes were visualized with fluorescein-labeled anti-dinitrophenyl Fab fragment (Molecular Probes) and rhodamine-labeled anti-digoxigenin Fab fragment (Boehringer-Mannheim GmbH). The green and red signals were quantitated from 10 metaphase images, where the background was subtracted and the strongest signal was set to 100%. The mean signal intensities are shown with the standard errors of the means.

Techniques Used: Polymerase Chain Reaction, Generated, In Situ Hybridization, Labeling

Incubation:

Article Title: In Situ Evaluation of Oxidative Stress in Rat Fatty Liver Induced by a Methionine- and Choline-Deficient Diet
Article Snippet: Endogenous peroxidase activity was blocked with a solution of 3% H2 O2 in 10% methanol for 20 min. After two washing instances in PBS for 5 min, nonspecific binding sites were blocked with 2.5% normal horse serum in PBS for 1 h. Further block was made with 5% skim milk and 1.5% NaCl in PBS for 30 min. .. The sections were then incubated with a rabbit monoclonal anti-dinitrophenyl (DNP) antibody (SP-0603; Vector Laboratories) diluted 1 : 100 in 2.5% normal horse serum at 4°C for 1 h. After two further 5 min washing instances in PBS, the sections were incubated with ImmPRESS HRP anti-rabbit Ig (peroxidase) Polymer Detection Kit (MP-7401; Vector Laboratories, Burlingame, CA 94010, USA) for 30 min at room temperature. .. Sections were washed twice in PBS for 5 min and incubated for 3 min with liquid DAB + chromogen system (K3468; DAKO, Carpinteria, California, USA).

Article Title: Large-scale profiling of noncoding RNA function in yeast
Article Snippet: Coverslips were then washed three times with 1ml blocking buffer and incubated for 2 hours with Alexa Fluor 488-conjugated anti-HRP antibody (Jackson Immuno Research) diluted 1:100 with 250μl blocking buffer. .. For combined co-localization detection of DIG- and DNP-labelled probes coverslips were incubated for 2 hrs with goat anti-DIG antibodies (Vector Laboratories) and rabbit anti-DNP (Vector Laboratories) diluted to 1:500 with 250μl blocking buffer. .. Coverslips were then washed three times with 1ml blocking buffer and incubated for 2 hours with mouse anti-rabbit Alexa Fluor 488 antibody (Jackson Immuno Research) and mouse anti-goat Alexa Fluor 647 antibody (Jackson Immuno Research) each diluted 1:100 with 250μl blocking buffer.

Article Title: Ectodermal P2X receptor function plays a pivotal role in craniofacial development of the zebrafish
Article Snippet: Plasmids were linearized with the appropriate restriction enzyme and cRNA preparation was performed using the AmpliScribe Transcription Kit (EpiCentre, Madison, WI). cRNAs were synthesized in the presence of digoxigenin-UTP (Roche) or modified by covalent modification using either d -nitrophenol (DNP) or fluorescein with the LabelIt Kit (Mirus Bio Corporation, Madison, WI). cRNAs were incubated in the presence of whole embryos at 65°C overnight in hybridization buffer (50% formamide, 5X SSC, 50 μg/ml heparin, 500 μg/ml tRNA, 9.2 mM citric acid and 0.1% Tween). .. Embryos were washed and incubated with alkaline-phosphatase-conjugated anti-DNP (Vector Laboratories, Burlingame, CA), anti-digoxigenin, or anti-fluorescein antibody (Roche). ..

Article Title: Long-Range Signaling Activation and Local Inhibition Separate the Mesoderm and Endoderm Lineages
Article Snippet: Hybridization with an additional riboprobe labeled with dinitrophenol (DNP)-11-UTP (Perkin Elmer, #NEL555001EA) allowed two targets to be visualized simultaneously. .. Embryos were then incubated overnight at 4°C with anti-Dig-AP as for standard WISH, or anti-Dig-HRP (Roche, #1207733910, 1:500), anti-DNP-HRP (Perkin Elmer, #FP1129) or anti-DNP-AP (Vector labs MB-3100, 1:1000) antibodies, followed by extensive washes in PTW. .. To detect HRP, embryos were incubated with tyramide (Sigma, #T2879) coupled to either fluorescein-NHS ester (Thermo Scientific, #46410) or Cy5 mono NHS ester (Sigma, #PA15101) for 25 min in the dark in PTW.

Blocking Assay:

Concentration Assay:

Article Title: PCR-generated padlock probes detect single nucleotide variation in genomic DNA
Article Snippet: .. Reacted probes were visualized by adding fluorophore-conjugated Fab fragments directed against digoxigenin and dinitrophenyl at a concentration of 4 ng/µl or 2 ng/µl fluorescein-labeled avidin (Vector Laboratories Inc., Burlingame, CA) in 2× SSC and 0.05% Tween-20 (70 µl of solution for each slide covered with a 60 × 24 mm coverslip). .. The slides were incubated at 37°C for 30 min and then washed with gentle agitation twice in 2× SSC and 0.05% Tween-20 for 7 min each time.

Avidin-Biotin Assay:

Synthesized:

Article Title: Region-specific regulation of stem cell-driven regeneration in tapeworms
Article Snippet: After rehydration, samples were permeabilized in 10 μg/mL Proteinase-K/0.1% SDS/PBSTx for 30 min, washed into 0.1 M Triethanolamine pH7-8 (TEA), 2.5 μL/mL acetic anhydride was added for 5 min with vigorous swirling, acetic anhydride step was repeated, washed in PBSTx, and post-fixed in 4% formaldehyde/PBSTx for 10 min. Probe synthesis, hybridization, and staining were performed as previously described ( ) using probe concentrations at ~50 ng/mL for 16–48 hr at 56°C. .. All probes were synthesized with either DIG or DNP haptens and detected using the following antibodies, all at 1:2000: anti-DIG-AP (Sigma), anti-DIG-POD (Sigma), anti-DNP-HRP (Vector Labs). .. Colorimetric development was done using NBT (Roche)/BCIP (Sigma) or with Fast-Blue (Sigma) ( ).