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Sony sony sh800z sorter
Sony Sh800z Sorter, supplied by Sony, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sony sh800z sorter/product/Sony
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sony sh800z sorter - by Bioz Stars, 2021-05
86/100 stars

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Expressing:

Article Title: Patterned endogenous activity controls migration, morphogenesis and survival of adult-born neurons in the mouse olfactory bulb
Article Snippet: First, the upper myelin layer and then supernatant were removed, the pellet was resuspended in sorting buffer (dissection medium without DNase I) and centrifuged once again for 10 min at 800 g and 4°C. .. Adult-born cells expressing Twitch-2B were separated by fluorescence-activated cell sorting on a Sony SH800Z sorter (Sony Biotechnology Inc, Surrey, UK) immediately after staining samples with propidium iodide (PI). ..

Fluorescence:

Article Title: Patterned endogenous activity controls migration, morphogenesis and survival of adult-born neurons in the mouse olfactory bulb
Article Snippet: First, the upper myelin layer and then supernatant were removed, the pellet was resuspended in sorting buffer (dissection medium without DNase I) and centrifuged once again for 10 min at 800 g and 4°C. .. Adult-born cells expressing Twitch-2B were separated by fluorescence-activated cell sorting on a Sony SH800Z sorter (Sony Biotechnology Inc, Surrey, UK) immediately after staining samples with propidium iodide (PI). ..

FACS:

Article Title: Patterned endogenous activity controls migration, morphogenesis and survival of adult-born neurons in the mouse olfactory bulb
Article Snippet: First, the upper myelin layer and then supernatant were removed, the pellet was resuspended in sorting buffer (dissection medium without DNase I) and centrifuged once again for 10 min at 800 g and 4°C. .. Adult-born cells expressing Twitch-2B were separated by fluorescence-activated cell sorting on a Sony SH800Z sorter (Sony Biotechnology Inc, Surrey, UK) immediately after staining samples with propidium iodide (PI). ..

Article Title: Isolation and Characterization of Single Cells from Zebrafish Embryos
Article Snippet: At 18 hpf, nkx2.5:ZsY embryos were dissociated into a single cell suspension then stained with Sytox Blue to exclude dead cells. .. Live, ZsYellow positive, Sytox Blue negative cells were FACS sorted using either a MoFloXDP or Sony SH800Z sorter equipped with 100 μm nozzle ( ). .. To assess purity of the sorted population and post-sort viability, an aliquot of sorted cells were stained with Sytox Blue and evaluated the percentage of events that fell within the original sorting gate ( ).

Staining:

Article Title: Patterned endogenous activity controls migration, morphogenesis and survival of adult-born neurons in the mouse olfactory bulb
Article Snippet: First, the upper myelin layer and then supernatant were removed, the pellet was resuspended in sorting buffer (dissection medium without DNase I) and centrifuged once again for 10 min at 800 g and 4°C. .. Adult-born cells expressing Twitch-2B were separated by fluorescence-activated cell sorting on a Sony SH800Z sorter (Sony Biotechnology Inc, Surrey, UK) immediately after staining samples with propidium iodide (PI). ..

Chromatin Immunoprecipitation:

Article Title: Tissue-specific profiling reveals distinctive regulatory architectures for ubiquitous, germline and somatic genes
Article Snippet: Finally, nuclei were filtered on 30 µm mesh (CellTrics 04-0042-2316) and stained with 0.025 µg/ml DAPI. .. Nuclear sorting was performed at 4 C using a Sony SH800Z sorter fitted with a 100 µm sorting chip and auto-calibrated. ..

Purification:

Article Title: Allospecific Memory B Cell Responses are Dependent on Autophagy
Article Snippet: Primary murine B cells were obtained from freshly isolated splenic single cell suspensions using EasySep™ Mouse B Cell Isolation Kit STEMCELL Technologies (Cambridge, MA) per manufacturer’s instructions. .. Purified YFP+ and YFPneg AID-CreERT2-eYFP B cells were enriched as above and then sorted to > 90% purity on a SONY SH800Z sorter (San Jose, CA) prior to ELISPOT. .. For human B cell experiments, peripheral blood mononuclear cells (PBMC) were isolated as previously described from leukopacs of anonymous donors purchased from the New York Blood Bank.

Enzyme-linked Immunospot:

Article Title: Allospecific Memory B Cell Responses are Dependent on Autophagy
Article Snippet: Primary murine B cells were obtained from freshly isolated splenic single cell suspensions using EasySep™ Mouse B Cell Isolation Kit STEMCELL Technologies (Cambridge, MA) per manufacturer’s instructions. .. Purified YFP+ and YFPneg AID-CreERT2-eYFP B cells were enriched as above and then sorted to > 90% purity on a SONY SH800Z sorter (San Jose, CA) prior to ELISPOT. .. For human B cell experiments, peripheral blood mononuclear cells (PBMC) were isolated as previously described from leukopacs of anonymous donors purchased from the New York Blood Bank.

Isolation:

Article Title: Molecular transitions in early progenitors during human cord blood hematopoiesis
Article Snippet: For mast cell progenitors, MNCs in single‐cell suspensions were stained with the following antibodies: APC mouse anti‐human CD34 (BD #560940, clone 581), APC/Cy7 anti‐human CD45RA antibody (BioLegend #304127, clone HI100), BV421 mouse anti‐human CD117 (BD #562435, clone YB5.B8), PE anti‐human Fc ε RI α antibody (BioLegend #334609, clone AER‐37), and FITC Mouse Anti‐Human CD71 (BD #561939, clone M‐A712). .. We gated CD34+ CD117+ Fc ε RI α + CD45RA− and CD34+ CD117+ Fc ε RI α + CD45RA+ cells on the SONY SH800Z sorter, with CD71 index recorded, and single cells were isolated into individual wells on 96‐well plates. .. Cells were immediately lysed and mRNAs were released when single cells were sorted into wells with 5× Maxima reverse transcription buffer, dNTP mixture, RNase inhibitors (SUPERase In RNase Inhibitor, Thermo Fisher Scientific #AM2696), RT primers and water.

Cell Culture:

Article Title: BCL11A interacts with SOX2 to control the expression of epigenetic regulators in lung squamous carcinoma
Article Snippet: H520, H1792 and LK2 cells were transfected with 4 μg of respective vector using Lipofectamine 3000 or Lipofectamine LTX (Invitrogen). .. Cells were treated with G418 (400 μg ml−1 ) (Gibco) for 5 days after which GFP+ve cell were sorted using the Sony SH800Z sorter and cultured. .. Doxycycline inducible shRNA-mediated KD SETD8 shRNA (TRCN0000359304 and TRCN0000359373), SKIL shRNA (TRCN0000431894 and TRCN0000424201); and TBX2 shRNA (TRCN0000232147 and TRCN0000232146) were cloned into pLKO-Tet-On vector .

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    Sony sh800z cell sorter
    C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an <t>SH800Z</t> cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p
    Sh800z Cell Sorter, supplied by Sony, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sh800z cell sorter/product/Sony
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sh800z cell sorter - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Sony sh800z sorter
    C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an <t>SH800Z</t> cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p
    Sh800z Sorter, supplied by Sony, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sh800z sorter/product/Sony
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sh800z sorter - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an SH800Z cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p

    Journal: Scientific Reports

    Article Title: An inhibitor of fibroblast growth factor receptor-1 (FGFR1) promotes late-stage terminal differentiation from NGN3+ pancreatic endocrine progenitors

    doi: 10.1038/srep35908

    Figure Lengend Snippet: C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an SH800Z cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p

    Article Snippet: The number of Venus- and mCherry-expressing cells was analysed in an SH800Z cell sorter (Sony, Tokyo, Japan).

    Techniques: Quantitative RT-PCR, Expressing