1 step cov kit (Solis BioDyne)


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1 Step Cov Kit, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1 step cov kit/product/Solis BioDyne
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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1) Product Images from "Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2"
Article Title: Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2
Journal: Virology Journal
doi: 10.1186/s12985-021-01559-3

Figure Legend Snippet: CT value overview for 3600 clinical specimens tested with the RKI/ZBS1 SARS-CoV-2 protocol. CT values for the four PCR assays included in the RKI/ZBS1 SARS-CoV-2 protocol as determined for the first 3600 specimens. Red symbols show the SARS-CoV-2 assays (negatives not shown), blue squares the inhibition control KoMa and green circles the nucleic acid detection by c-myc amplification. The constant detection of the inhibition control KoMa can be seen, and the scattering of the nucleic acid content demonstrated by c-myc as well as the similar CT values for the E-Gene and the orf1ab assay
Techniques Used: Polymerase Chain Reaction, Inhibition, Amplification

Figure Legend Snippet: RKI/ZBS1 SARS-CoV-2 protocol performance on different PCR cyclers. To show that the presented protocol can be run on several cyclers, we used 10 clinical samples of different RNA load, negative as well as positive controls and set up one master mix that was distributed to the different cyclers as shown above. Different colors represent 10 different samples used for comparison. Mean values of duplicates are shown, also for SARS-CoV-2-positive controls (crosses); negative controls are not shown
Techniques Used: Polymerase Chain Reaction

Figure Legend Snippet: Correlation between the internal KoMa control and SARS-CoV-2 genome load in clinical specimens. For the 424 SARS-CoV-2-positive specimens the CT values were grouped as indicated and plotted against the CT value for the inhibition control KoMa. While in specimens that are highly positive for SARS-CoV-2 KoMa does not give a signal reliably, in specimens with genome loads close to the detection limit as well as in negative samples, KoMa is constantly amplified, showing the efficiency of the nucleic acid extraction and possible inhibitory effects of the sample matrix. Detectability increases significantly with E-Gene CT values higher than 25 (Mann Whitney p
Techniques Used: Inhibition, Amplification, MANN-WHITNEY

Figure Legend Snippet: Principle of the RKI/ZBS1 protocol for the real-time PCR detection of SARS-CoV-2. RKI/ZBS1 SARS-CoV-2 protocol
Techniques Used: Real-time Polymerase Chain Reaction

Figure Legend Snippet: RKI/ZBS1 SARS-CoV-2 protocol performance with different PCR master mixes. In total 7 PCR mixes were compared with the RKI/ZBS1 SARS-CoV-2 protocol on a Bio-Rad CFX96 cycler. 10 clinical specimens of different RNA load and negative as well as positive controls were set up. Means of duplicate CT values are plotted against the respective master mix. Positive controls (crosses) are shown only for the SARS-CoV-2 assays E-Gene and orf1ab; negative controls are not shown
Techniques Used: Polymerase Chain Reaction
2) Product Images from "Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2"
Article Title: Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2
Journal: Virology Journal
doi: 10.1186/s12985-021-01559-3

Figure Legend Snippet: CT value overview for 3600 clinical specimens tested with the RKI/ZBS1 SARS-CoV-2 protocol. CT values for the four PCR assays included in the RKI/ZBS1 SARS-CoV-2 protocol as determined for the first 3600 specimens. Red symbols show the SARS-CoV-2 assays (negatives not shown), blue squares the inhibition control KoMa and green circles the nucleic acid detection by c-myc amplification. The constant detection of the inhibition control KoMa can be seen, and the scattering of the nucleic acid content demonstrated by c-myc as well as the similar CT values for the E-Gene and the orf1ab assay
Techniques Used: Polymerase Chain Reaction, Inhibition, Amplification

Figure Legend Snippet: RKI/ZBS1 SARS-CoV-2 protocol performance on different PCR cyclers. To show that the presented protocol can be run on several cyclers, we used 10 clinical samples of different RNA load, negative as well as positive controls and set up one master mix that was distributed to the different cyclers as shown above. Different colors represent 10 different samples used for comparison. Mean values of duplicates are shown, also for SARS-CoV-2-positive controls (crosses); negative controls are not shown
Techniques Used: Polymerase Chain Reaction

Figure Legend Snippet: Correlation between the internal KoMa control and SARS-CoV-2 genome load in clinical specimens. For the 424 SARS-CoV-2-positive specimens the CT values were grouped as indicated and plotted against the CT value for the inhibition control KoMa. While in specimens that are highly positive for SARS-CoV-2 KoMa does not give a signal reliably, in specimens with genome loads close to the detection limit as well as in negative samples, KoMa is constantly amplified, showing the efficiency of the nucleic acid extraction and possible inhibitory effects of the sample matrix. Detectability increases significantly with E-Gene CT values higher than 25 (Mann Whitney p
Techniques Used: Inhibition, Amplification, MANN-WHITNEY

Figure Legend Snippet: Principle of the RKI/ZBS1 protocol for the real-time PCR detection of SARS-CoV-2. RKI/ZBS1 SARS-CoV-2 protocol
Techniques Used: Real-time Polymerase Chain Reaction

Figure Legend Snippet: RKI/ZBS1 SARS-CoV-2 protocol performance with different PCR master mixes. In total 7 PCR mixes were compared with the RKI/ZBS1 SARS-CoV-2 protocol on a Bio-Rad CFX96 cycler. 10 clinical specimens of different RNA load and negative as well as positive controls were set up. Means of duplicate CT values are plotted against the respective master mix. Positive controls (crosses) are shown only for the SARS-CoV-2 assays E-Gene and orf1ab; negative controls are not shown
Techniques Used: Polymerase Chain Reaction
3) Product Images from "Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2"
Article Title: Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2
Journal: Virology Journal
doi: 10.1186/s12985-021-01559-3

Figure Legend Snippet: CT value overview for 3600 clinical specimens tested with the RKI/ZBS1 SARS-CoV-2 protocol. CT values for the four PCR assays included in the RKI/ZBS1 SARS-CoV-2 protocol as determined for the first 3600 specimens. Red symbols show the SARS-CoV-2 assays (negatives not shown), blue squares the inhibition control KoMa and green circles the nucleic acid detection by c-myc amplification. The constant detection of the inhibition control KoMa can be seen, and the scattering of the nucleic acid content demonstrated by c-myc as well as the similar CT values for the E-Gene and the orf1ab assay
Techniques Used: Polymerase Chain Reaction, Inhibition, Amplification

Figure Legend Snippet: RKI/ZBS1 SARS-CoV-2 protocol performance on different PCR cyclers. To show that the presented protocol can be run on several cyclers, we used 10 clinical samples of different RNA load, negative as well as positive controls and set up one master mix that was distributed to the different cyclers as shown above. Different colors represent 10 different samples used for comparison. Mean values of duplicates are shown, also for SARS-CoV-2-positive controls (crosses); negative controls are not shown
Techniques Used: Polymerase Chain Reaction

Figure Legend Snippet: Correlation between the internal KoMa control and SARS-CoV-2 genome load in clinical specimens. For the 424 SARS-CoV-2-positive specimens the CT values were grouped as indicated and plotted against the CT value for the inhibition control KoMa. While in specimens that are highly positive for SARS-CoV-2 KoMa does not give a signal reliably, in specimens with genome loads close to the detection limit as well as in negative samples, KoMa is constantly amplified, showing the efficiency of the nucleic acid extraction and possible inhibitory effects of the sample matrix. Detectability increases significantly with E-Gene CT values higher than 25 (Mann Whitney p
Techniques Used: Inhibition, Amplification, MANN-WHITNEY

Figure Legend Snippet: Principle of the RKI/ZBS1 protocol for the real-time PCR detection of SARS-CoV-2. RKI/ZBS1 SARS-CoV-2 protocol
Techniques Used: Real-time Polymerase Chain Reaction

Figure Legend Snippet: RKI/ZBS1 SARS-CoV-2 protocol performance with different PCR master mixes. In total 7 PCR mixes were compared with the RKI/ZBS1 SARS-CoV-2 protocol on a Bio-Rad CFX96 cycler. 10 clinical specimens of different RNA load and negative as well as positive controls were set up. Means of duplicate CT values are plotted against the respective master mix. Positive controls (crosses) are shown only for the SARS-CoV-2 assays E-Gene and orf1ab; negative controls are not shown
Techniques Used: Polymerase Chain Reaction
4) Product Images from "First report on the Latvian SARS-CoV-2 isolate genetic diversity"
Article Title: First report on the Latvian SARS-CoV-2 isolate genetic diversity
Journal: medRxiv
doi: 10.1101/2020.09.08.20190504

Figure Legend Snippet: Ten most frequently mutated genome positions among Latvian SARS-CoV-2 isolates (n=133). X axis is discrete and shows genome position corresponding to one of the ten most frequent mutated positions (ordered in descending order). Y axis represents mutation occurrence among the samples (same as numbers within the respective upper boundary of bars) at a given position. Labels in the middle of bars represent the nucleotide change at a given position and its effect on the respective protein amino acid sequence. Color coding is based on the variant class and bars are colored according to the legend. Note: bars representing different mutations of the same locus are stacked (position 28881).
Techniques Used: Mutagenesis, Sequencing, Variant Assay

Figure Legend Snippet: Evolutionary relationships of 133 sequenced Latvian and Wuhan-Hu-1 SARS-CoV-2 isolates. The evolutionary history was inferred using the Maximum-likelihood method allowing for polytomies. The tree is rooted at Wuhan-Hu-1 reference sequence. The tree is drawn to scale, branch lengths correspond to nucleotide substitutions. The analysis involved 134 nucleotide sequences (133 Latvian SARS-CoV-2 isolates and Wuhan-Hu-1 reference sequence). There were a total of 29903 positions in the final dataset. Node labels are colored according to the GISAID major clade of particular isolate, green – GR, yellow – GH, red – G, blue – L, purple – O (other), black – Wuhan-Hu-1 reference sequence.
Techniques Used: Sequencing

Figure Legend Snippet: Root-to-tip regression analysis of 133 Latvian SARS-CoV-2 isolates and Wuhan-Hu-1 sequence.
Techniques Used: Sequencing

Figure Legend Snippet: Maximum clade credibility tree (mean node heights) estimated from the completely sequenced Latvian isolates (n=133) and Wuhan-Hu-1 isolate. Node labels are colored according to the GISAID major clade of particular isolate, green – GR, yellow – GH, red – G, blue – L, purple – O (other), black – Wuhan-Hu-1 reference sequence. The tree is timescaled and axis represents time in a decimal year notation (one months is ∼0.08333 of a year and one day is approximately 0.00274 of a year). Nodes are colored according to their respective posterior probabilities in gradient from blue (lowest value) to red (highest value). Dated node bars represent 95% highest posterior density intervals and are shown for the selected nodes.
Techniques Used: Sequencing

Figure Legend Snippet: Methodological strategy plan for SARS-CoV-2 genome analysis based on different next-generation sequencing methods.
Techniques Used: Next-Generation Sequencing

Figure Legend Snippet: Mutational landscape of Latvian SARS-CoV-2 isolates. Y axis shows the mutated position of a reference SARS-CoV-2 genome. X axis shows the cumulative mutation count at a given position. Number to the right of bars indicate cumulative mutation count at a given position and bars are color-coded according to the protein that the corresponding site participates in encoding. Note, that Y axis is discrete and only positions with mutations documented in local isolates are shown.
Techniques Used: Mutagenesis

Figure Legend Snippet: Daily numbers of positive COVID-19 cases (A) and tests performed (B) in Latvia. X axis is the same for both tiles and represents daily time series from 28 th of February, 2020 to 11 th of September, 2020. The red vertical line indicates the date of the first COVID-19 case registered in Latvia. A) Y value represents the total number of positive cases registered on a given day. Blue area shows the number of successfully sequenced isolates, while the red area represents the positive cases not sequenced during this study. B) Y value represents the number of tests carried out on a given date in Latvia.
Techniques Used:
5) Product Images from "First Report on the Latvian SARS-CoV-2 Isolate Genetic Diversity"
Article Title: First Report on the Latvian SARS-CoV-2 Isolate Genetic Diversity
Journal: Frontiers in Medicine
doi: 10.3389/fmed.2021.626000

Figure Legend Snippet: Distribution of sequenced SARS-CoV-2 isolates by clades in major regions of the world, worldwide, and in Latvia. y -axis depicts cumulative complete SARS-CoV-2 genome count (with unambiguous collection date) from a particular region and has different scale within the subplots. x -axis is the same for all subplots and depicts sampling time-series from 24th of December, 2019 till 12th of September, 2020.
Techniques Used: Sampling

Figure Legend Snippet: Daily numbers of positive COVID-19 cases (A) and tests performed (B) in Latvia. x -axis is the same for both tiles and represents daily time series from 28th of February, 2020 to 11th of September, 2020. The red vertical line indicates the date of the first COVID-19 case registered in Latvia. (A) Y value represents the total number of positive cases registered on a given day. Blue area shows the number of only successfully sequenced isolates, while the red area represents the positive cases not sequenced during this study. (B) Y value represents the number of tests carried out on a given date in Latvia.
Techniques Used:

Figure Legend Snippet: Maximum clade credibility tree (mean node heights) estimated from the completely sequenced Latvian isolates ( n = 133) and Wuhan-Hu-1 isolate. Node labels are colored according to the GISAID major clade of particular isolate, as follows: green, GR; yellow, GH; red, G; blue, L; purple, O (other); black, Wuhan-Hu-1 reference sequence. The tree is time scaled and axis represents time in a decimal year notation (1 months is ~0.08333 of a year and 1 day is ~0.00274 of a year). Nodes are colored according to their respective posterior probabilities in gradient from blue (lowest value) to red (highest value). Dated node bars represent 95% highest posterior density intervals and are shown for the selected nodes.
Techniques Used: Sequencing

Figure Legend Snippet: Evolutionary relationships of 133 sequenced Latvian and Wuhan-Hu-1 SARS-CoV-2 isolates. The evolutionary history was inferred using the Maximum-likelihood method allowing for polytomies. The tree is rooted at Wuhan-Hu-1 reference sequence. The tree is drawn to scale; branch lengths correspond to nucleotide substitutions. The analysis involved 134 nucleotide sequences (133 Latvian SARS-CoV-2 isolates and Wuhan-Hu-1 reference sequence). There were a total of 29,903 positions in the final dataset. Node labels are colored according to the GISAID major clade of particular isolate, as follows: green, GR; yellow, GH; red, G; blue, L; purple, O (other); black, Wuhan-Hu-1 reference sequence.
Techniques Used: Sequencing