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Waters Corporation solid phase extraction
Solid Phase Extraction, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 98/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/solid phase extraction/product/Waters Corporation
Average 98 stars, based on 38 article reviews
Price from $9.99 to $1999.99
solid phase extraction - by Bioz Stars, 2020-04
98/100 stars

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Centrifugation:

Article Title: Coleus amboinicus (Lour.) leaves as a modulator of ruminal methanogenesis and biohydrogenation in vitro
Article Snippet: Subsequently, the solid extract was dissolved in a small volume of acidified water containing 0.2% formic acid, and purified on a solid phase extraction using C18 Sep-Pak cartridge (1 cm3 , 360 mg, Waters Corp., Milford, MA). .. The phenolics fraction was re-evaporated and dissolved in 1 mL of 80% methanol followed by centrifugation (23,000 × g , 5 min).

Article Title: Acetyl-CoA carboxylase 1 and 2 inhibition ameliorates steatosis and hepatic fibrosis in a MC4R knockout murine model of nonalcoholic steatohepatitis
Article Snippet: .. After centrifugation, the supernatant was subjected to solid phase extraction using the Oasis HLB Extraction Cartridge (Waters, Massachusetts, US). .. The analyte was eluted with acetonitrile supplemented with dibutylammonium acetate (Tokyo Chemical Industries, Tokyo, Japan), followed by rinsing of the column with ultrapurified water.

Incubation:

Article Title: Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074
Article Snippet: In each case, 40 Erlenmeyer flasks (250 ml), each containing 50 ml of medium, were inoculated with spores and incubated in an orbital shaker (Climo-Shaker ISF4-X, Adolf Kühner AG, Basel, Switzerland) at 30°C and 250 r.p.m. during 3 days. .. After every purification step, the collected compounds were diluted fourfold with water and were desalted and concentrated by solid-phase extraction (Sep-Pak C18, Waters).

Mass Spectrometry:

Article Title: Coleus amboinicus (Lour.) leaves as a modulator of ruminal methanogenesis and biohydrogenation in vitro
Article Snippet: Subsequently, the solid extract was dissolved in a small volume of acidified water containing 0.2% formic acid, and purified on a solid phase extraction using C18 Sep-Pak cartridge (1 cm3 , 360 mg, Waters Corp., Milford, MA). .. The phytochemicals were determined using an ultrahigh resolution mass spectrometer system (Dionex UltiMate 3000RS, Thermo Scientific, Darmstadt, Germany) equipped with ultra-performance liquid chromatography ( UPLC ) and a charged aerosol detector interfaced with a high-resolution quadrupole time-of-flight ( TOF ) mass spectrometer (Impact II, Bruker Daltonik GmbH, Bremen, Germany).

BIA-KA:

Article Title: A thiol probe for measuring unfolded protein load and proteostasis in cells
Article Snippet: The protein concentration in the supernatant was determined by a BCA assay (Catalogue number: 23225, Thermo Fischer Scientific) using BSA as the mass standard. .. The resultant peptides were desalted by solid-phase extraction, by acidification in 1% v/v formic acid; pre-washing the cartridge (Oasis HLB 1 cc Vac Cartridge, product number 186000383, Waters Corp., USA) with 1 ml of 80% v/v acetonitrile (ACN) containing 0.1% v/v trifluoroacetic acid (TFA) and washing with 1.2 ml of 0.1% v/v TFA three times by applying to a vacuum manifold; loading the sample on the cartridge and washing with 1.5 ml of 0.1% v/v TFA; eluting the sample with 0.8 ml of 80% v/v ACN containing 0.1% v/v TFA and collecting in 1.5 ml microcentrifuge tubes; then lyophilization by freeze drying (Virtis, SP Scientific).

High Performance Liquid Chromatography:

Article Title: Synthesis of fluorine-18 labeled rhodamine B: A potential PET myocardial perfusion imaging agent
Article Snippet: .. The final product, [18 F]FERhB, was obtained in an overall radiochemical yield of 35% after purification by solid-phase extraction (silica gel Sep-Pak® cartridge (Waters), CHCl3 :methanol, 4:1) or semi-preparative HPLC (tR =13.2 min). .. The total reaction time using this method, including the drying of the [18 F]fluoride and preparative HPLC, is approximately 90 min.

Article Title: Genetically Modified Bacterial Strains and Novel Bacterial Artificial Chromosome Shuttle Vectors for Constructing Environmental Libraries and Detecting Heterologous Natural Products in Multiple Expression Hosts
Article Snippet: Paragraph title: Preparation of extracts and HPLC analysis. ... Extracts were filtered, concentrated (N2 stream; Pierce Reacti-Therm), cleaned by solid-phase extraction (Waters SepPak C18 cartridges; 3 ml; 200 mg), and dried under an N2 stream.

Article Title: Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074
Article Snippet: The compounds of interest were purified by preparative HPLC using a SunFire C18 column (10 μm, 10 × 250 mm; Waters). .. After every purification step, the collected compounds were diluted fourfold with water and were desalted and concentrated by solid-phase extraction (Sep-Pak C18, Waters).

Chromatography:

Article Title: Vitamin D binding protein rs7041 genotype alters vitamin D metabolism in pregnant women
Article Snippet: Methanol (1 ml) was added to 0.4 g of homogenized placental tissues, along with 600 µl of acetonitrile, 1 ml of methyl tertiary butyl ether, and 25 µl of internal standard containing 215 M D3 -25(OH)D3 (IsoSciences, King of Prussia, PA, USA) and 1.7 M D6 -24,25(OH)2D3 (Toronto Research Chemicals, Toronto, ON, Canada) A liquid–liquid extraction was followed by a solid-phase extraction on Oasis HLB cartridges (3 cm3 /60 mg; Waters, Milford, MA, USA) and subsequent derivatization with 50 µl of 0.1 mg/ml 4-(2-(6,7-dimethoxy-4-methyl-3-oxo-3,4-dihydroquinoxalinyl)ethyl)-1,2,4-triazoline-3,5-dione (DMEQ-TAD; Key Synthesis, Wynnewood, PA, USA) ( , ). .. Finally, extracts were resuspended in 110 µl of 60:40 methanol:water, injected onto a pentafluorophenyl column (Accucore PFP 2.1 × 100, 2.6 mm; Thermo Fisher Scientific) with a matching guard column at 45°C, and separated by liquid chromatography–MS/MS ( ).

Protease Inhibitor:

Article Title: A thiol probe for measuring unfolded protein load and proteostasis in cells
Article Snippet: Cells were then detached using a cell scraper, lysed using RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, Protease inhibitor, 1% v/v NP40, 2 mM EDTA and DNAase1), then centrifuged at 16,000 g for 10 min. .. The resultant peptides were desalted by solid-phase extraction, by acidification in 1% v/v formic acid; pre-washing the cartridge (Oasis HLB 1 cc Vac Cartridge, product number 186000383, Waters Corp., USA) with 1 ml of 80% v/v acetonitrile (ACN) containing 0.1% v/v trifluoroacetic acid (TFA) and washing with 1.2 ml of 0.1% v/v TFA three times by applying to a vacuum manifold; loading the sample on the cartridge and washing with 1.5 ml of 0.1% v/v TFA; eluting the sample with 0.8 ml of 80% v/v ACN containing 0.1% v/v TFA and collecting in 1.5 ml microcentrifuge tubes; then lyophilization by freeze drying (Virtis, SP Scientific).

other:

Article Title: The Relationship Between Urinary Total Polyphenols and the Frailty Phenotype in a Community-Dwelling Older Population: The InCHIANTI Study
Article Snippet: The analysis of total polyphenols in baseline urine samples was performed using the Folin–Ciocalteu reagent (Sigma-Aldrich, St. Louis, MO) after solid-phase extraction using Oasis MAX 96-well plates (Waters, Milford, MA) according to our previous methodology ( ).

Protein Concentration:

Article Title: A thiol probe for measuring unfolded protein load and proteostasis in cells
Article Snippet: The protein concentration in the supernatant was determined by a BCA assay (Catalogue number: 23225, Thermo Fischer Scientific) using BSA as the mass standard. .. The resultant peptides were desalted by solid-phase extraction, by acidification in 1% v/v formic acid; pre-washing the cartridge (Oasis HLB 1 cc Vac Cartridge, product number 186000383, Waters Corp., USA) with 1 ml of 80% v/v acetonitrile (ACN) containing 0.1% v/v trifluoroacetic acid (TFA) and washing with 1.2 ml of 0.1% v/v TFA three times by applying to a vacuum manifold; loading the sample on the cartridge and washing with 1.5 ml of 0.1% v/v TFA; eluting the sample with 0.8 ml of 80% v/v ACN containing 0.1% v/v TFA and collecting in 1.5 ml microcentrifuge tubes; then lyophilization by freeze drying (Virtis, SP Scientific).

Injection:

Article Title: Acetyl-CoA carboxylase 1 and 2 inhibition ameliorates steatosis and hepatic fibrosis in a MC4R knockout murine model of nonalcoholic steatohepatitis
Article Snippet: After centrifugation, the supernatant was subjected to solid phase extraction using the Oasis HLB Extraction Cartridge (Waters, Massachusetts, US). .. An aliquot of 10 μL was injected into an LC-MS/MS system.

Article Title: Vitamin D binding protein rs7041 genotype alters vitamin D metabolism in pregnant women
Article Snippet: Methanol (1 ml) was added to 0.4 g of homogenized placental tissues, along with 600 µl of acetonitrile, 1 ml of methyl tertiary butyl ether, and 25 µl of internal standard containing 215 M D3 -25(OH)D3 (IsoSciences, King of Prussia, PA, USA) and 1.7 M D6 -24,25(OH)2D3 (Toronto Research Chemicals, Toronto, ON, Canada) A liquid–liquid extraction was followed by a solid-phase extraction on Oasis HLB cartridges (3 cm3 /60 mg; Waters, Milford, MA, USA) and subsequent derivatization with 50 µl of 0.1 mg/ml 4-(2-(6,7-dimethoxy-4-methyl-3-oxo-3,4-dihydroquinoxalinyl)ethyl)-1,2,4-triazoline-3,5-dione (DMEQ-TAD; Key Synthesis, Wynnewood, PA, USA) ( , ). .. Finally, extracts were resuspended in 110 µl of 60:40 methanol:water, injected onto a pentafluorophenyl column (Accucore PFP 2.1 × 100, 2.6 mm; Thermo Fisher Scientific) with a matching guard column at 45°C, and separated by liquid chromatography–MS/MS ( ).

Article Title: Pilot Pharmacokinetic Study of Human Immunodeficiency Virus-Infected Patients Receiving Tenofovir Disoproxil Fumarate (TDF): Investigation of Systemic and Intracellular Interactions between TDF and Abacavir, Lamivudine, or Lopinavir-Ritonavir ▿
Article Snippet: Solid-phase extraction was performed on Oasis MCX 60-mg, 3-ml cartridges (Waters, Saint-Quentin-en-Yvelines, France) using a positive-pressure processor (Speedisk 48; Mallinckrodt Baker, Paris, France). .. Extracts reconstituted with 200 μl of mobile phase A (see below) were held at +4°C in the autosampler, and 10 μl was injected into the LC-MS-MS system (Alliance 2795-Quattro Micro; Waters, France).

Isolation:

Article Title: Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074
Article Snippet: Paragraph title: Isolation of 6-epi -alteramides ... After every purification step, the collected compounds were diluted fourfold with water and were desalted and concentrated by solid-phase extraction (Sep-Pak C18, Waters).

Labeling:

Article Title: [18F]Fluorophenylazocarboxylates: Design and Synthesis of Potential Radioligands for Dopamine D3 and μ-Opioid Receptor
Article Snippet: The labeling to yield the versatile prosthetic group [ 18 F]2a is therefore possible under the conditions of the “minimalist procedure.” The “minimalist procedure” though requires a higher precursor amount of twice or thrice the concentration necessary for the conventional approach to enable labeling with fluorine-18. .. Precursor 1 is though very easily separated from [ 18 F]2a through solid-phase extraction (tC18, Waters), and the higher excess of the unreacted precursor did not influence the subsequent coupling of [ 18 F]2a to give the potential opioid receptor PET ligand [ 18 F]4a ( ).

Article Title: Impact of a High-fat Diet on Tissue Acyl-CoA and Histone Acetylation Levels *
Article Snippet: The weighed samples were added to 1 ml of thawed 13 C3 15 N1 -labeled acyl-CoA internal standard in 1.5-ml Eppendorf tubes on ice. .. The supernatant was purified by solid-phase extraction using Oasis HLB 1-ml (30 mg) solid phase extraction columns (Waters, Milford, MA).

Mouse Assay:

Article Title: Acetyl-CoA carboxylase 1 and 2 inhibition ameliorates steatosis and hepatic fibrosis in a MC4R knockout murine model of nonalcoholic steatohepatitis
Article Snippet: The liver was also harvested 1 hour after the last drug administration in the repeated dosing study using WD-fed MC4R KO mice. .. After centrifugation, the supernatant was subjected to solid phase extraction using the Oasis HLB Extraction Cartridge (Waters, Massachusetts, US).

Positron Emission Tomography:

Article Title: [18F]Fluorophenylazocarboxylates: Design and Synthesis of Potential Radioligands for Dopamine D3 and μ-Opioid Receptor
Article Snippet: .. Precursor 1 is though very easily separated from [ 18 F]2a through solid-phase extraction (tC18, Waters), and the higher excess of the unreacted precursor did not influence the subsequent coupling of [ 18 F]2a to give the potential opioid receptor PET ligand [ 18 F]4a ( ). ..

Purification:

Article Title: Synthesis of fluorine-18 labeled rhodamine B: A potential PET myocardial perfusion imaging agent
Article Snippet: .. The final product, [18 F]FERhB, was obtained in an overall radiochemical yield of 35% after purification by solid-phase extraction (silica gel Sep-Pak® cartridge (Waters), CHCl3 :methanol, 4:1) or semi-preparative HPLC (tR =13.2 min). .. The total reaction time using this method, including the drying of the [18 F]fluoride and preparative HPLC, is approximately 90 min.

Article Title: Coleus amboinicus (Lour.) leaves as a modulator of ruminal methanogenesis and biohydrogenation in vitro
Article Snippet: .. Subsequently, the solid extract was dissolved in a small volume of acidified water containing 0.2% formic acid, and purified on a solid phase extraction using C18 Sep-Pak cartridge (1 cm3 , 360 mg, Waters Corp., Milford, MA). ..

Article Title: Impact of a High-fat Diet on Tissue Acyl-CoA and Histone Acetylation Levels *
Article Snippet: .. The supernatant was purified by solid-phase extraction using Oasis HLB 1-ml (30 mg) solid phase extraction columns (Waters, Milford, MA). ..

Article Title: Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074
Article Snippet: .. After every purification step, the collected compounds were diluted fourfold with water and were desalted and concentrated by solid-phase extraction (Sep-Pak C18, Waters). ..

Liquid Chromatography:

Article Title: Coleus amboinicus (Lour.) leaves as a modulator of ruminal methanogenesis and biohydrogenation in vitro
Article Snippet: Subsequently, the solid extract was dissolved in a small volume of acidified water containing 0.2% formic acid, and purified on a solid phase extraction using C18 Sep-Pak cartridge (1 cm3 , 360 mg, Waters Corp., Milford, MA). .. The phytochemicals were determined using an ultrahigh resolution mass spectrometer system (Dionex UltiMate 3000RS, Thermo Scientific, Darmstadt, Germany) equipped with ultra-performance liquid chromatography ( UPLC ) and a charged aerosol detector interfaced with a high-resolution quadrupole time-of-flight ( TOF ) mass spectrometer (Impact II, Bruker Daltonik GmbH, Bremen, Germany).

Software:

Article Title: Genetically Modified Bacterial Strains and Novel Bacterial Artificial Chromosome Shuttle Vectors for Constructing Environmental Libraries and Detecting Heterologous Natural Products in Multiple Expression Hosts
Article Snippet: Inertsil ODS-3 (5 μm; 120 Å, 150 by 4.6 mm; GL Sciences) and Polaris C-18A (5 μm; 120 Å; 4.6 by 150 mm; Metachem) columns were used for analytical reverse-phase high-pressure liquid chromatography (HPLC) on a Waters 600 system with 996 PDA detector (210 to 610 nm; 1.2-nm resolution; Millennium 4.0 software). .. Extracts were filtered, concentrated (N2 stream; Pierce Reacti-Therm), cleaned by solid-phase extraction (Waters SepPak C18 cartridges; 3 ml; 200 mg), and dried under an N2 stream.

Low Protein Binding:

Article Title: Pitfalls and solutions of the fully-automated radiosynthesis of [11C]metoclopramide
Article Snippet: SepPak® C18 plus cartridges for solid-phase extraction were purchased from Waters (Waters® Associates Milford, MA, USA). .. Low-protein binding Millex GS® 0.22 μm sterile filters were purchased from Millipore® (Bedford, MA, USA).

Sample Prep:

Article Title: A thiol probe for measuring unfolded protein load and proteostasis in cells
Article Snippet: Paragraph title: Proteomics sample preparation ... The resultant peptides were desalted by solid-phase extraction, by acidification in 1% v/v formic acid; pre-washing the cartridge (Oasis HLB 1 cc Vac Cartridge, product number 186000383, Waters Corp., USA) with 1 ml of 80% v/v acetonitrile (ACN) containing 0.1% v/v trifluoroacetic acid (TFA) and washing with 1.2 ml of 0.1% v/v TFA three times by applying to a vacuum manifold; loading the sample on the cartridge and washing with 1.5 ml of 0.1% v/v TFA; eluting the sample with 0.8 ml of 80% v/v ACN containing 0.1% v/v TFA and collecting in 1.5 ml microcentrifuge tubes; then lyophilization by freeze drying (Virtis, SP Scientific).

Evaporation:

Article Title: [18F]Fluorophenylazocarboxylates: Design and Synthesis of Potential Radioligands for Dopamine D3 and μ-Opioid Receptor
Article Snippet: The subsequent labeling proceeded after the evaporation of the solvent system and addition of dry acetonitrile with an RCY of about 75% after 30 s at 85 °C. .. Precursor 1 is though very easily separated from [ 18 F]2a through solid-phase extraction (tC18, Waters), and the higher excess of the unreacted precursor did not influence the subsequent coupling of [ 18 F]2a to give the potential opioid receptor PET ligand [ 18 F]4a ( ).

Concentration Assay:

Article Title: [18F]Fluorophenylazocarboxylates: Design and Synthesis of Potential Radioligands for Dopamine D3 and μ-Opioid Receptor
Article Snippet: The labeling to yield the versatile prosthetic group [ 18 F]2a is therefore possible under the conditions of the “minimalist procedure.” The “minimalist procedure” though requires a higher precursor amount of twice or thrice the concentration necessary for the conventional approach to enable labeling with fluorine-18. .. Precursor 1 is though very easily separated from [ 18 F]2a through solid-phase extraction (tC18, Waters), and the higher excess of the unreacted precursor did not influence the subsequent coupling of [ 18 F]2a to give the potential opioid receptor PET ligand [ 18 F]4a ( ).

Thin Layer Chromatography:

Article Title: Synthesis of fluorine-18 labeled rhodamine B: A potential PET myocardial perfusion imaging agent
Article Snippet: The final product, [18 F]FERhB, was obtained in an overall radiochemical yield of 35% after purification by solid-phase extraction (silica gel Sep-Pak® cartridge (Waters), CHCl3 :methanol, 4:1) or semi-preparative HPLC (tR =13.2 min). .. The identity of the product was confirmed by analytical HPLC and TLC using non-radioactive compound 2′-fluoroethyl rhodamine B as a reference.

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  • 93
    Waters Corporation solid phase extraction spe column
    LC-MS chromatograms of <t>SK14-061a</t> in urine. Urine samples were collected in a metabolic cage and were pretreated on an <t>SPE</t> column (Waters Oasis MCX 96 well plate) for extracting SK14-061a as described in the Materials and Methods section.
    Solid Phase Extraction Spe Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solid phase extraction spe column/product/Waters Corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    solid phase extraction spe column - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    80
    Waters Corporation solid phase extraction c18 solid phase extraction spe columns
    Purification and identification of S-geranylgeranyl-L-glutathione as an endogenous compound active on P2RY8. (a) Diagram of P2RY8 ligand bioassay, depicting migration inhibition of P2RY8 + WEHI-231 cells by extracts containing P2RY8 ligand. (b) Flow cytometry plots of cells from the bottom well of the bioassay described in (a), using mouse liver extract or diluted bile. (c) P2RY8 ligand bioassay of culture media from the indicated cell lines (n=5). (d) P2RY8 ligand bioassay of media from Hepa1-6 cells incubated with the indicated agents (10 μM statin, 100 μM mevalonate (MVA), 100 μM GG-PP or DMSO vehicle) (n=8, one-way ANOVA with Bonferroni’s multiple comparisons test). (e) Diagram of 7-step purification strategy to identify the bioactive compound in bile; asterisks indicate steps used for culture supernatants. Right panel shows scheme for MS detection of candidate ions. (f) Full MS scan (Q1) of purified fractions from the indicated conditions, in positive ion mode. (g) Chemical structure of S-geranylgeranyl-L-glutathione (Ggg). (h) Positive ion mode MS/MS spectra of the 580.3 ion from purified bile (left) and from synthesized Ggg (right). (i) LC-MS/MS quantification of Ggg in <t>C18</t> solid phase extracts <t>(SPE)</t> of mouse spleen (n=8) and lymph node (n=5), human tonsil (n=6), or mouse bile (n=6). (j) P2RY8 ligand bioassay of C18 SPE concentrates from 500 mg of spleen or tonsil (n=5). Data are representative of or pooled from 3 (b,c,d,h,j,), 2 (i) or 1 (f) experiments. Graphs depict mean with s.d. and each point represents a biological replicate.
    Solid Phase Extraction C18 Solid Phase Extraction Spe Columns, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solid phase extraction c18 solid phase extraction spe columns/product/Waters Corporation
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    solid phase extraction c18 solid phase extraction spe columns - by Bioz Stars, 2020-04
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    91
    Waters Corporation c18 solid phase extraction fs dacs
    Molecular mass distribution of fractions obtained from <t>F-DACS</t> after <t>C18</t> SPE and precipitation in 67% (v/v) ethanol, analysed using HPSEC-RI. F0s and F30s were 67% (v/v) ethanol-soluble fractions, F0i and F30i were 67% (v/v) ethanol-insoluble fractions
    C18 Solid Phase Extraction Fs Dacs, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 solid phase extraction fs dacs/product/Waters Corporation
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c18 solid phase extraction fs dacs - by Bioz Stars, 2020-04
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    Image Search Results


    LC-MS chromatograms of SK14-061a in urine. Urine samples were collected in a metabolic cage and were pretreated on an SPE column (Waters Oasis MCX 96 well plate) for extracting SK14-061a as described in the Materials and Methods section.

    Journal: PLoS ONE

    Article Title: Pharmacokinetic properties of a novel inosine analog, 4′-cyano-2′-deoxyinosine, after oral administration in rats

    doi: 10.1371/journal.pone.0198636

    Figure Lengend Snippet: LC-MS chromatograms of SK14-061a in urine. Urine samples were collected in a metabolic cage and were pretreated on an SPE column (Waters Oasis MCX 96 well plate) for extracting SK14-061a as described in the Materials and Methods section.

    Article Snippet: For the quantitation of SK14-061a using LC-MS, plasma samples were pretreated using a solid phase extraction (SPE) column, a Waters Oasis MCX 96 well plate (Waters, Milford, USA).

    Techniques: Liquid Chromatography with Mass Spectroscopy

    LC-MS chromatograms of (A) SK14-061a spiked with water, (B) SK14-061a spiked with plasma after a methanol treatment, (C) SK14-061a spiked with plasma after an acetonitrile treatment, (D) blank plasma after SPE treatment, and (E) SK14-061a spiked with plasma after SPE treatment. All SK14-061a samples contained 500 ng/mL in concentration before either reverse liquid-liquid extraction or SPE. The SK14-061a were detected by the LC-MS as described in the Materials and Methods section.

    Journal: PLoS ONE

    Article Title: Pharmacokinetic properties of a novel inosine analog, 4′-cyano-2′-deoxyinosine, after oral administration in rats

    doi: 10.1371/journal.pone.0198636

    Figure Lengend Snippet: LC-MS chromatograms of (A) SK14-061a spiked with water, (B) SK14-061a spiked with plasma after a methanol treatment, (C) SK14-061a spiked with plasma after an acetonitrile treatment, (D) blank plasma after SPE treatment, and (E) SK14-061a spiked with plasma after SPE treatment. All SK14-061a samples contained 500 ng/mL in concentration before either reverse liquid-liquid extraction or SPE. The SK14-061a were detected by the LC-MS as described in the Materials and Methods section.

    Article Snippet: For the quantitation of SK14-061a using LC-MS, plasma samples were pretreated using a solid phase extraction (SPE) column, a Waters Oasis MCX 96 well plate (Waters, Milford, USA).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Concentration Assay

    (A) Time course for the plasma concentration of ETV after an oral administration of ETV alone (closed circle) or combination with SK14-061a (open circle) at doses of 1 mg/kg in rats. (B) Time course for the concentration of SK14-061a in plasma after the oral administration of SK14-061a alone (closed circle) or combination with ETV (open circle) at doses of 1 mg/kg in rats. Venous blood samples were collected at 15 min, 45 min, 90 min, 3, 6 and 9 hr after administration. The SK14-061a and ETV concentrations in plasma were measured using LC-MS with the SPE method. The data for SK14-061a alone are quoted from Fig 3 . The values are the mean ± SD. (n = 4).

    Journal: PLoS ONE

    Article Title: Pharmacokinetic properties of a novel inosine analog, 4′-cyano-2′-deoxyinosine, after oral administration in rats

    doi: 10.1371/journal.pone.0198636

    Figure Lengend Snippet: (A) Time course for the plasma concentration of ETV after an oral administration of ETV alone (closed circle) or combination with SK14-061a (open circle) at doses of 1 mg/kg in rats. (B) Time course for the concentration of SK14-061a in plasma after the oral administration of SK14-061a alone (closed circle) or combination with ETV (open circle) at doses of 1 mg/kg in rats. Venous blood samples were collected at 15 min, 45 min, 90 min, 3, 6 and 9 hr after administration. The SK14-061a and ETV concentrations in plasma were measured using LC-MS with the SPE method. The data for SK14-061a alone are quoted from Fig 3 . The values are the mean ± SD. (n = 4).

    Article Snippet: For the quantitation of SK14-061a using LC-MS, plasma samples were pretreated using a solid phase extraction (SPE) column, a Waters Oasis MCX 96 well plate (Waters, Milford, USA).

    Techniques: Concentration Assay, Liquid Chromatography with Mass Spectroscopy

    Time course for the plasma concentration of SK14-061a after oral administration at a dose of 1 mg/kg in rats. Venous blood samples were collected at 15 min, 45 min, 90 min, 3, 4.5, 6 and 9 hr after oral administration. The SK14-061a concentrations in plasma were measured by LC-MS combined with the SPE method. The values are the mean ± SD. (n = 4).

    Journal: PLoS ONE

    Article Title: Pharmacokinetic properties of a novel inosine analog, 4′-cyano-2′-deoxyinosine, after oral administration in rats

    doi: 10.1371/journal.pone.0198636

    Figure Lengend Snippet: Time course for the plasma concentration of SK14-061a after oral administration at a dose of 1 mg/kg in rats. Venous blood samples were collected at 15 min, 45 min, 90 min, 3, 4.5, 6 and 9 hr after oral administration. The SK14-061a concentrations in plasma were measured by LC-MS combined with the SPE method. The values are the mean ± SD. (n = 4).

    Article Snippet: For the quantitation of SK14-061a using LC-MS, plasma samples were pretreated using a solid phase extraction (SPE) column, a Waters Oasis MCX 96 well plate (Waters, Milford, USA).

    Techniques: Concentration Assay, Liquid Chromatography with Mass Spectroscopy

    LC-MS chromatograms of SK14-061a in urine. Urine samples were collected in a metabolic cage and were pretreated on an SPE column (Waters Oasis MCX 96 well plate) for extracting SK14-061a as described in the Materials and Methods section.

    Journal: PLoS ONE

    Article Title: Pharmacokinetic properties of a novel inosine analog, 4′-cyano-2′-deoxyinosine, after oral administration in rats

    doi: 10.1371/journal.pone.0198636

    Figure Lengend Snippet: LC-MS chromatograms of SK14-061a in urine. Urine samples were collected in a metabolic cage and were pretreated on an SPE column (Waters Oasis MCX 96 well plate) for extracting SK14-061a as described in the Materials and Methods section.

    Article Snippet: For the quantitation of SK14-061a using LC-MS, plasma samples were pretreated using a solid phase extraction (SPE) column, a Waters Oasis MCX 96 well plate (Waters, Milford, USA).

    Techniques: Liquid Chromatography with Mass Spectroscopy

    Purification and identification of S-geranylgeranyl-L-glutathione as an endogenous compound active on P2RY8. (a) Diagram of P2RY8 ligand bioassay, depicting migration inhibition of P2RY8 + WEHI-231 cells by extracts containing P2RY8 ligand. (b) Flow cytometry plots of cells from the bottom well of the bioassay described in (a), using mouse liver extract or diluted bile. (c) P2RY8 ligand bioassay of culture media from the indicated cell lines (n=5). (d) P2RY8 ligand bioassay of media from Hepa1-6 cells incubated with the indicated agents (10 μM statin, 100 μM mevalonate (MVA), 100 μM GG-PP or DMSO vehicle) (n=8, one-way ANOVA with Bonferroni’s multiple comparisons test). (e) Diagram of 7-step purification strategy to identify the bioactive compound in bile; asterisks indicate steps used for culture supernatants. Right panel shows scheme for MS detection of candidate ions. (f) Full MS scan (Q1) of purified fractions from the indicated conditions, in positive ion mode. (g) Chemical structure of S-geranylgeranyl-L-glutathione (Ggg). (h) Positive ion mode MS/MS spectra of the 580.3 ion from purified bile (left) and from synthesized Ggg (right). (i) LC-MS/MS quantification of Ggg in C18 solid phase extracts (SPE) of mouse spleen (n=8) and lymph node (n=5), human tonsil (n=6), or mouse bile (n=6). (j) P2RY8 ligand bioassay of C18 SPE concentrates from 500 mg of spleen or tonsil (n=5). Data are representative of or pooled from 3 (b,c,d,h,j,), 2 (i) or 1 (f) experiments. Graphs depict mean with s.d. and each point represents a biological replicate.

    Journal: Nature

    Article Title: S-geranylgeranyl-L-glutathione is a ligand for human B-cell confinement receptor P2RY8

    doi: 10.1038/s41586-019-1003-z

    Figure Lengend Snippet: Purification and identification of S-geranylgeranyl-L-glutathione as an endogenous compound active on P2RY8. (a) Diagram of P2RY8 ligand bioassay, depicting migration inhibition of P2RY8 + WEHI-231 cells by extracts containing P2RY8 ligand. (b) Flow cytometry plots of cells from the bottom well of the bioassay described in (a), using mouse liver extract or diluted bile. (c) P2RY8 ligand bioassay of culture media from the indicated cell lines (n=5). (d) P2RY8 ligand bioassay of media from Hepa1-6 cells incubated with the indicated agents (10 μM statin, 100 μM mevalonate (MVA), 100 μM GG-PP or DMSO vehicle) (n=8, one-way ANOVA with Bonferroni’s multiple comparisons test). (e) Diagram of 7-step purification strategy to identify the bioactive compound in bile; asterisks indicate steps used for culture supernatants. Right panel shows scheme for MS detection of candidate ions. (f) Full MS scan (Q1) of purified fractions from the indicated conditions, in positive ion mode. (g) Chemical structure of S-geranylgeranyl-L-glutathione (Ggg). (h) Positive ion mode MS/MS spectra of the 580.3 ion from purified bile (left) and from synthesized Ggg (right). (i) LC-MS/MS quantification of Ggg in C18 solid phase extracts (SPE) of mouse spleen (n=8) and lymph node (n=5), human tonsil (n=6), or mouse bile (n=6). (j) P2RY8 ligand bioassay of C18 SPE concentrates from 500 mg of spleen or tonsil (n=5). Data are representative of or pooled from 3 (b,c,d,h,j,), 2 (i) or 1 (f) experiments. Graphs depict mean with s.d. and each point represents a biological replicate.

    Article Snippet: Solid phase extraction C18 solid phase extraction (SPE) columns were purchased from Waters (Sep-Pak, 10g-35cc and 500mg-6cc versions).

    Techniques: Purification, Migration, Inhibition, Flow Cytometry, Cytometry, Incubation, Mass Spectrometry, Synthesized, Liquid Chromatography with Mass Spectroscopy

    Ggt5 metabolizes Ggg and regulates P2RY8 function in vivo. (a, b) Flow cytometry plots (a) and summary data (b) of P2RY8 ligand bioassay of supernatants from the indicated cells overexpressing Ggt5 or empty vector (EV), cultured for 18 hr with Ggg or vehicle (HEK293T n=6, Hepa1-6 n=4 biological replicates, one-way ANOVA with Bonferroni’s multiple comparisons test). Graphs depict mean with s.d. (c) Diagram of Ggg conversion into S-geranylgeranyl-L-Cys-Gly (GG-Cys-Gly). (d) Positive precursor ion scan for m/z 179 to identify ions producing [Cys-Gly] + fragments, from purified supernatants of HEK293T cells overexpressing EV or Ggt5 and incubated with Ggg. (e) LC-MS/MS MRM for Ggg and GG-Cys-Gly in a mixture of 100 nM Ggg and GG-Cys-Gly, or in C18 SPE concentrates of mouse spleen. (f) Immunohistochemistry for GGT5 or CR2 (brown) in serial sections of tonsil counterstained with hematoxylin (blue). (g) RNAscope for Ggt5 mRNA (red) in mouse lymph node GCs and primary follicles, counterstained with IgD (brown). Serial sections were stained for Cr1 (blue) and IgD (brown). (h) Immunofluorescence for P2RY8-overexpressing B cells (GFP, green) co-transferred with EV or Ggt5-overexpressing B cells, in unimmunized mice without GCs (top), and in GCs (Cr1, red) of immunized mice (bottom), relative to endogenous B cells (IgD, blue). Data are representative of or pooled from 3 (a,b,e) or 2 (d,h) experiments, 3 (h) or 5 (f,g) biological replicates. Scale bars, 100 μm.

    Journal: Nature

    Article Title: S-geranylgeranyl-L-glutathione is a ligand for human B-cell confinement receptor P2RY8

    doi: 10.1038/s41586-019-1003-z

    Figure Lengend Snippet: Ggt5 metabolizes Ggg and regulates P2RY8 function in vivo. (a, b) Flow cytometry plots (a) and summary data (b) of P2RY8 ligand bioassay of supernatants from the indicated cells overexpressing Ggt5 or empty vector (EV), cultured for 18 hr with Ggg or vehicle (HEK293T n=6, Hepa1-6 n=4 biological replicates, one-way ANOVA with Bonferroni’s multiple comparisons test). Graphs depict mean with s.d. (c) Diagram of Ggg conversion into S-geranylgeranyl-L-Cys-Gly (GG-Cys-Gly). (d) Positive precursor ion scan for m/z 179 to identify ions producing [Cys-Gly] + fragments, from purified supernatants of HEK293T cells overexpressing EV or Ggt5 and incubated with Ggg. (e) LC-MS/MS MRM for Ggg and GG-Cys-Gly in a mixture of 100 nM Ggg and GG-Cys-Gly, or in C18 SPE concentrates of mouse spleen. (f) Immunohistochemistry for GGT5 or CR2 (brown) in serial sections of tonsil counterstained with hematoxylin (blue). (g) RNAscope for Ggt5 mRNA (red) in mouse lymph node GCs and primary follicles, counterstained with IgD (brown). Serial sections were stained for Cr1 (blue) and IgD (brown). (h) Immunofluorescence for P2RY8-overexpressing B cells (GFP, green) co-transferred with EV or Ggt5-overexpressing B cells, in unimmunized mice without GCs (top), and in GCs (Cr1, red) of immunized mice (bottom), relative to endogenous B cells (IgD, blue). Data are representative of or pooled from 3 (a,b,e) or 2 (d,h) experiments, 3 (h) or 5 (f,g) biological replicates. Scale bars, 100 μm.

    Article Snippet: Solid phase extraction C18 solid phase extraction (SPE) columns were purchased from Waters (Sep-Pak, 10g-35cc and 500mg-6cc versions).

    Techniques: In Vivo, Flow Cytometry, Cytometry, Plasmid Preparation, Cell Culture, Purification, Incubation, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Immunohistochemistry, Staining, Immunofluorescence, Mouse Assay

    FACS gating strategy and purity. (a) Flow cytometry plots showing gating scheme used to sort the indicated subsets from human tonsil, along with post-sort purity. (b) For each bioassay performed, representative experiments are graphed as % of input migration for both the transduced and un-transduced WEHI-231 subsets indicated. For Fig 1j , the C18 SPE concentrates exhibited inhibition of overall migration likely due to slight toxicity; however, P2RY8 + cells were more selectively inhibited compared to P2RY8 - cells. The baseline migration across experiments differs based on the growth state of the WEHI-231 cells. Graphs depict mean with s.d.

    Journal: Nature

    Article Title: S-geranylgeranyl-L-glutathione is a ligand for human B-cell confinement receptor P2RY8

    doi: 10.1038/s41586-019-1003-z

    Figure Lengend Snippet: FACS gating strategy and purity. (a) Flow cytometry plots showing gating scheme used to sort the indicated subsets from human tonsil, along with post-sort purity. (b) For each bioassay performed, representative experiments are graphed as % of input migration for both the transduced and un-transduced WEHI-231 subsets indicated. For Fig 1j , the C18 SPE concentrates exhibited inhibition of overall migration likely due to slight toxicity; however, P2RY8 + cells were more selectively inhibited compared to P2RY8 - cells. The baseline migration across experiments differs based on the growth state of the WEHI-231 cells. Graphs depict mean with s.d.

    Article Snippet: Solid phase extraction C18 solid phase extraction (SPE) columns were purchased from Waters (Sep-Pak, 10g-35cc and 500mg-6cc versions).

    Techniques: FACS, Flow Cytometry, Cytometry, Migration, Inhibition

    Molecular mass distribution of fractions obtained from F-DACS after C18 SPE and precipitation in 67% (v/v) ethanol, analysed using HPSEC-RI. F0s and F30s were 67% (v/v) ethanol-soluble fractions, F0i and F30i were 67% (v/v) ethanol-insoluble fractions

    Journal: Biotechnology for Biofuels

    Article Title: Characterisation of non-degraded oligosaccharides in enzymatically hydrolysed and fermented, dilute ammonia-pretreated corn stover for ethanol production

    doi: 10.1186/s13068-017-0803-3

    Figure Lengend Snippet: Molecular mass distribution of fractions obtained from F-DACS after C18 SPE and precipitation in 67% (v/v) ethanol, analysed using HPSEC-RI. F0s and F30s were 67% (v/v) ethanol-soluble fractions, F0i and F30i were 67% (v/v) ethanol-insoluble fractions

    Article Snippet: C18 solid-phase extraction Fs-DACS (1.2 g) was dissolved in 10 mL water and loaded on a C18 SPE column (35 cc, 10 g, Waters Corp., Milford, MA, USA).

    Techniques: