sodium pyrophosphate  (Roche)


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    Structured Review

    Roche sodium pyrophosphate
    Sodium Pyrophosphate, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 650 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium pyrophosphate/product/Roche
    Average 94 stars, based on 650 article reviews
    Price from $9.99 to $1999.99
    sodium pyrophosphate - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Protease Inhibitor:

    Article Title: FAM83F regulates canonical Wnt signalling through an interaction with CK1α
    Article Snippet: .. For whole cell protein extractions, cell pellets were resuspended in total lysis buffer (20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% (v/v) Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3VO4, 1x complete EDTA-free protease inhibitor cocktail (Roche)). .. Lysates were incubated on ice for 30 min and vortexed regularly then clarified at 13000 rpm for 20 min. For cellular fractionation, cell pellets were washed in PBS twice, scraped in PBS, pelleted and then separated into cytoplasmic, nuclear and membrane lysates using a subcellular protein fractionation kit (Thermo Fisher Scientific) following the manufacturer’s protocol.

    Article Title: EphA2 is a Mediator of Vemurafenib Resistance and a Novel Therapeutic Target in Melanoma
    Article Snippet: .. Cells were lysed in 20 mM HEPES, pH 7.5, 150 mM NaCl, 1% NP-40, 10 mM tetrasodium pyrophosphate, 100 mM NaF, 17.5 mM 1-glycerophosphate buffer supplemented with Complete Mini Protease Inhibitor Cocktail tablets (Roche Applied Sciences, Indianapolis, IN). .. Samples separated by SDS-PAGE were transferred to nitrocellulose membranes, blocked with 5% bovine serum albumin (w/v) at room temperature for 1 hr, and incubated with primary antibodies (1:1000 dilution) at 4°C overnight.

    Article Title: Impaired Priming and Activation of the Neutrophil NADPH Oxidase in Patients with IRAK4- or NEMO-deficiency *
    Article Snippet: .. The treated cells were centrifuged at 1000 × g for 10 min at 4°C and the pellets were lysed in Cell Lysis Buffer (Cell Signaling Technology, Inc., Danvers, MA) containing 20 mM Tris/HCl (pH 7.5), 150 mM NaCl, 1mM EGTA, 1 mM Na2 EDTA, 2.5 mM sodium pyrophosphate, 1 mM sodium orthovanadate, 1% Triton X-100, 1 μg/ml leupeptin, 1 mM β-glycerophosphate and Complete Protease Inhibitor Cocktail (Roche Diagnostics Corp., Indianapolis, IN). .. Immunoprecipitation was performed by incubating cleared supernatant with 5 μg mouse anti-p47phox antibody for 16 hrs at 4°C with constant rotation.

    Article Title: The HIV-1 Vpu transmembrane domain topology and formation of a hydrophobic interface with BST-2 are critical for Vpu-mediated BST-2 downregulation
    Article Snippet: .. Virus replication and releaseHEK293T cells were cotransfected with either pNL4-3 or pNL-ΔVpu-IRES-GFP, together with expression vectors for EGFP (control) or EGFP-tagged Vpu, F1 or F2, and BST-2-HA After 48 hr cells were harvested in lysis buffer containing 150 mM NaCl, 20 mM Tris pH 7.5, 1 mM EDTA, 1% Triton X-100, 1 mM EGTA, 2.5 mM Sodium pyrophosphate, 1 mM Na3VO4 and protease inhibitor cocktail (Roche, Germany). .. After centrifugation at 16,000xg for 10 min, the protein concentrations in clarified lysates were estimated using the Bradford assay (Bio-Rad).

    Article Title: Metformin, an AMPK Activator, Inhibits Activation of FLSs but Promotes HAPLN1 Secretion
    Article Snippet: .. Medium was replaced in the wells with fresh medium containing metformin (5 mM) or saline for 36 h. After aspiration of the medium, cell monolayers were rinsed with 1 mL of ice-cold PBS and lysed in 80 μL of lysis buffer (20 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% [v/v] Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate) supplemented with fresh 1 mM Na3 VO4 and 1 mM dithiothreitol containing 1× protease inhibitor cocktail (Roche Applied Science, Basel, Switzerland). .. Supernatants were collected and the protein concentrations were measured using a Bradford assay (Thermo Fisher Scientific, MA, USA).

    Mouse Assay:

    Article Title: Activation of Neuronal NMDA Receptors Triggers Transient ATP-Mediated Microglial Process Outgrowth
    Article Snippet: .. Additional brain slices from mice, from which slices were used for imaging and fixation, were homogenized using lysis buffer containing the following (in m m ): 100 Tris (pH 7.0), 2 EGTA, 5 EDTA, 30 NaF, 20 sodium pyrophosphate, and 0.5% NP40 with phosphatase and protease inhibitors (Roche) and centrifuged at 13,000 × g for 20 min at 4°C. .. Protein concentrations of the lysates were determined by Bradford assay using a DC protein assay dye (Bio-Rad) and 20 μg of protein (diluted in 2× Laemmli buffer with 5% β-mercaptoethanol and boiled for 5 min) was used for SDS/PAGE (10% precast gel; Lonza).

    Imaging:

    Article Title: Activation of Neuronal NMDA Receptors Triggers Transient ATP-Mediated Microglial Process Outgrowth
    Article Snippet: .. Additional brain slices from mice, from which slices were used for imaging and fixation, were homogenized using lysis buffer containing the following (in m m ): 100 Tris (pH 7.0), 2 EGTA, 5 EDTA, 30 NaF, 20 sodium pyrophosphate, and 0.5% NP40 with phosphatase and protease inhibitors (Roche) and centrifuged at 13,000 × g for 20 min at 4°C. .. Protein concentrations of the lysates were determined by Bradford assay using a DC protein assay dye (Bio-Rad) and 20 μg of protein (diluted in 2× Laemmli buffer with 5% β-mercaptoethanol and boiled for 5 min) was used for SDS/PAGE (10% precast gel; Lonza).

    Expressing:

    Article Title: The HIV-1 Vpu transmembrane domain topology and formation of a hydrophobic interface with BST-2 are critical for Vpu-mediated BST-2 downregulation
    Article Snippet: .. Virus replication and releaseHEK293T cells were cotransfected with either pNL4-3 or pNL-ΔVpu-IRES-GFP, together with expression vectors for EGFP (control) or EGFP-tagged Vpu, F1 or F2, and BST-2-HA After 48 hr cells were harvested in lysis buffer containing 150 mM NaCl, 20 mM Tris pH 7.5, 1 mM EDTA, 1% Triton X-100, 1 mM EGTA, 2.5 mM Sodium pyrophosphate, 1 mM Na3VO4 and protease inhibitor cocktail (Roche, Germany). .. After centrifugation at 16,000xg for 10 min, the protein concentrations in clarified lysates were estimated using the Bradford assay (Bio-Rad).

    Western Blot:

    Article Title: Disruption of folate metabolism causes germline epigenetic instability and distinguishes HIRA as a biomarker of maternal transgenerational epigenetic inheritance
    Article Snippet: .. Western blotting Embryos and placentas at E10.5 were homogenised in lysis buffer (20 mM Tris [pH 7.5], 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerolphosphate, 1 mM Na3 VO4 and complete mini EDTA-free proteases inhibitor cocktail [Roche Diagnostics]) with Lysing Matrix D ceramic beads (MP Biomedical) using a MagNA Lyser (Roche Diagnostics) at 5,500 rpm for 20 seconds. ..

    Lysis:

    Article Title: FAM83F regulates canonical Wnt signalling through an interaction with CK1α
    Article Snippet: .. For whole cell protein extractions, cell pellets were resuspended in total lysis buffer (20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% (v/v) Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3VO4, 1x complete EDTA-free protease inhibitor cocktail (Roche)). .. Lysates were incubated on ice for 30 min and vortexed regularly then clarified at 13000 rpm for 20 min. For cellular fractionation, cell pellets were washed in PBS twice, scraped in PBS, pelleted and then separated into cytoplasmic, nuclear and membrane lysates using a subcellular protein fractionation kit (Thermo Fisher Scientific) following the manufacturer’s protocol.

    Article Title: Disruption of folate metabolism causes germline epigenetic instability and distinguishes HIRA as a biomarker of maternal transgenerational epigenetic inheritance
    Article Snippet: .. Western blotting Embryos and placentas at E10.5 were homogenised in lysis buffer (20 mM Tris [pH 7.5], 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerolphosphate, 1 mM Na3 VO4 and complete mini EDTA-free proteases inhibitor cocktail [Roche Diagnostics]) with Lysing Matrix D ceramic beads (MP Biomedical) using a MagNA Lyser (Roche Diagnostics) at 5,500 rpm for 20 seconds. ..

    Article Title: Impaired Priming and Activation of the Neutrophil NADPH Oxidase in Patients with IRAK4- or NEMO-deficiency *
    Article Snippet: .. The treated cells were centrifuged at 1000 × g for 10 min at 4°C and the pellets were lysed in Cell Lysis Buffer (Cell Signaling Technology, Inc., Danvers, MA) containing 20 mM Tris/HCl (pH 7.5), 150 mM NaCl, 1mM EGTA, 1 mM Na2 EDTA, 2.5 mM sodium pyrophosphate, 1 mM sodium orthovanadate, 1% Triton X-100, 1 μg/ml leupeptin, 1 mM β-glycerophosphate and Complete Protease Inhibitor Cocktail (Roche Diagnostics Corp., Indianapolis, IN). .. Immunoprecipitation was performed by incubating cleared supernatant with 5 μg mouse anti-p47phox antibody for 16 hrs at 4°C with constant rotation.

    Article Title: The HIV-1 Vpu transmembrane domain topology and formation of a hydrophobic interface with BST-2 are critical for Vpu-mediated BST-2 downregulation
    Article Snippet: .. Virus replication and releaseHEK293T cells were cotransfected with either pNL4-3 or pNL-ΔVpu-IRES-GFP, together with expression vectors for EGFP (control) or EGFP-tagged Vpu, F1 or F2, and BST-2-HA After 48 hr cells were harvested in lysis buffer containing 150 mM NaCl, 20 mM Tris pH 7.5, 1 mM EDTA, 1% Triton X-100, 1 mM EGTA, 2.5 mM Sodium pyrophosphate, 1 mM Na3VO4 and protease inhibitor cocktail (Roche, Germany). .. After centrifugation at 16,000xg for 10 min, the protein concentrations in clarified lysates were estimated using the Bradford assay (Bio-Rad).

    Article Title: Activation of Neuronal NMDA Receptors Triggers Transient ATP-Mediated Microglial Process Outgrowth
    Article Snippet: .. Additional brain slices from mice, from which slices were used for imaging and fixation, were homogenized using lysis buffer containing the following (in m m ): 100 Tris (pH 7.0), 2 EGTA, 5 EDTA, 30 NaF, 20 sodium pyrophosphate, and 0.5% NP40 with phosphatase and protease inhibitors (Roche) and centrifuged at 13,000 × g for 20 min at 4°C. .. Protein concentrations of the lysates were determined by Bradford assay using a DC protein assay dye (Bio-Rad) and 20 μg of protein (diluted in 2× Laemmli buffer with 5% β-mercaptoethanol and boiled for 5 min) was used for SDS/PAGE (10% precast gel; Lonza).

    Article Title: Metformin, an AMPK Activator, Inhibits Activation of FLSs but Promotes HAPLN1 Secretion
    Article Snippet: .. Medium was replaced in the wells with fresh medium containing metformin (5 mM) or saline for 36 h. After aspiration of the medium, cell monolayers were rinsed with 1 mL of ice-cold PBS and lysed in 80 μL of lysis buffer (20 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% [v/v] Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate) supplemented with fresh 1 mM Na3 VO4 and 1 mM dithiothreitol containing 1× protease inhibitor cocktail (Roche Applied Science, Basel, Switzerland). .. Supernatants were collected and the protein concentrations were measured using a Bradford assay (Thermo Fisher Scientific, MA, USA).

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  • 85
    Roche hek 293 lysis buffer
    Interaction of Hsp20 with AKAP-Lbc ( A ) Co-immunoprecipitation of Hsp20 with AKAP-Lbc in <t>HEK-293</t> cells. Plasmids encoding Hsp20–V5 and FLAG–AKAP-Lbc were co-transfected into cells. Cell lysates were immunoprecipitated with anti-FLAG antibodies or normal IgG, and immunoblotted with anti-Hsp20 antibodies (upper panel) or anti-FLAG antibodies (lower panel). ( B ) Reciprocal co-immunoprecipitation of AKAP-Lbc with Hsp20 in HEK-293 cells. Cell lysates were immunoprecipitated with anti-V5 antibodies or normal IgG and indicated proteins were identified by immunoblotting. ( C ) Endogenous co-immunoprecipitation of Hsp20 with AKAP-Lbc in neonatal rat ventricular cardiac myocytes. Lysates were immunoprecipitated with anti-AKAP-Lbc antibodies or isotype-matched IgG, and immunoblotted as shown. Molecular masses in kDa are indicated to the left-hand side of Western blots. ( D–F ) Co-distribution of Hsp20 and AKAP-Lbc in heart cells. ( D ) Immunofluorescent image (red) of neonatal rat ventricular cardiac myocytes labelled with anti-Hsp20 antibodies followed by Alexa Fluor ® , secondary antibodies. ( E ) Immunostaining of AKAP-Lbc (green). ( F ) Composite image (yellow). Scale bars represent 20 µM.
    Hek 293 Lysis Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek 293 lysis buffer/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hek 293 lysis buffer - by Bioz Stars, 2020-09
    85/100 stars
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    94
    Roche sodium pyrophosphate
    Interaction of Hsp20 with AKAP-Lbc ( A ) Co-immunoprecipitation of Hsp20 with AKAP-Lbc in <t>HEK-293</t> cells. Plasmids encoding Hsp20–V5 and FLAG–AKAP-Lbc were co-transfected into cells. Cell lysates were immunoprecipitated with anti-FLAG antibodies or normal IgG, and immunoblotted with anti-Hsp20 antibodies (upper panel) or anti-FLAG antibodies (lower panel). ( B ) Reciprocal co-immunoprecipitation of AKAP-Lbc with Hsp20 in HEK-293 cells. Cell lysates were immunoprecipitated with anti-V5 antibodies or normal IgG and indicated proteins were identified by immunoblotting. ( C ) Endogenous co-immunoprecipitation of Hsp20 with AKAP-Lbc in neonatal rat ventricular cardiac myocytes. Lysates were immunoprecipitated with anti-AKAP-Lbc antibodies or isotype-matched IgG, and immunoblotted as shown. Molecular masses in kDa are indicated to the left-hand side of Western blots. ( D–F ) Co-distribution of Hsp20 and AKAP-Lbc in heart cells. ( D ) Immunofluorescent image (red) of neonatal rat ventricular cardiac myocytes labelled with anti-Hsp20 antibodies followed by Alexa Fluor ® , secondary antibodies. ( E ) Immunostaining of AKAP-Lbc (green). ( F ) Composite image (yellow). Scale bars represent 20 µM.
    Sodium Pyrophosphate, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 711 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium pyrophosphate/product/Roche
    Average 94 stars, based on 711 article reviews
    Price from $9.99 to $1999.99
    sodium pyrophosphate - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Interaction of Hsp20 with AKAP-Lbc ( A ) Co-immunoprecipitation of Hsp20 with AKAP-Lbc in HEK-293 cells. Plasmids encoding Hsp20–V5 and FLAG–AKAP-Lbc were co-transfected into cells. Cell lysates were immunoprecipitated with anti-FLAG antibodies or normal IgG, and immunoblotted with anti-Hsp20 antibodies (upper panel) or anti-FLAG antibodies (lower panel). ( B ) Reciprocal co-immunoprecipitation of AKAP-Lbc with Hsp20 in HEK-293 cells. Cell lysates were immunoprecipitated with anti-V5 antibodies or normal IgG and indicated proteins were identified by immunoblotting. ( C ) Endogenous co-immunoprecipitation of Hsp20 with AKAP-Lbc in neonatal rat ventricular cardiac myocytes. Lysates were immunoprecipitated with anti-AKAP-Lbc antibodies or isotype-matched IgG, and immunoblotted as shown. Molecular masses in kDa are indicated to the left-hand side of Western blots. ( D–F ) Co-distribution of Hsp20 and AKAP-Lbc in heart cells. ( D ) Immunofluorescent image (red) of neonatal rat ventricular cardiac myocytes labelled with anti-Hsp20 antibodies followed by Alexa Fluor ® , secondary antibodies. ( E ) Immunostaining of AKAP-Lbc (green). ( F ) Composite image (yellow). Scale bars represent 20 µM.

    Journal: The Biochemical journal

    Article Title: The A-kinase-anchoring protein AKAP-Lbc facilitates cardioprotective PKA phosphorylation of Hsp20 on Ser16

    doi: 10.1042/BJ20120570

    Figure Lengend Snippet: Interaction of Hsp20 with AKAP-Lbc ( A ) Co-immunoprecipitation of Hsp20 with AKAP-Lbc in HEK-293 cells. Plasmids encoding Hsp20–V5 and FLAG–AKAP-Lbc were co-transfected into cells. Cell lysates were immunoprecipitated with anti-FLAG antibodies or normal IgG, and immunoblotted with anti-Hsp20 antibodies (upper panel) or anti-FLAG antibodies (lower panel). ( B ) Reciprocal co-immunoprecipitation of AKAP-Lbc with Hsp20 in HEK-293 cells. Cell lysates were immunoprecipitated with anti-V5 antibodies or normal IgG and indicated proteins were identified by immunoblotting. ( C ) Endogenous co-immunoprecipitation of Hsp20 with AKAP-Lbc in neonatal rat ventricular cardiac myocytes. Lysates were immunoprecipitated with anti-AKAP-Lbc antibodies or isotype-matched IgG, and immunoblotted as shown. Molecular masses in kDa are indicated to the left-hand side of Western blots. ( D–F ) Co-distribution of Hsp20 and AKAP-Lbc in heart cells. ( D ) Immunofluorescent image (red) of neonatal rat ventricular cardiac myocytes labelled with anti-Hsp20 antibodies followed by Alexa Fluor ® , secondary antibodies. ( E ) Immunostaining of AKAP-Lbc (green). ( F ) Composite image (yellow). Scale bars represent 20 µM.

    Article Snippet: Cells were transiently transfected using Transit-LT1 (Mirus) and harvested after 24 h in HEK-293 lysis buffer [25 mM Hepes, 50 mM NaCl, 2.5 mM EDTA, 50 mM NaF, 30 mM sodium pyrophosphate, 10% (v/v) glycerol and 1% (v/v) Triton X-100, pH 7.5] containing 1× complete EDTA-free protease inhibitor cocktail (Roche).

    Techniques: Immunoprecipitation, Transfection, Western Blot, Immunostaining