sodium deoxycholate (Thermo Fisher)
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Name:
Sodium Deoxycholate Detergent
Description:
Thermo Scientific Sodium Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions Features of Sodium Deoxycholate • Popular anionic bile acid detergent for many laboratory uses • Effective in disrupting and dissociating many types of protein interaction • Can be removed from solution by dialysis • Useful for elution or regeneration of certain kinds of affinity columns • High purity compound with low UV absorptivity Properties of Sodium Deoxycholate • Alternative Names Sodium deoxycholic acid deoxycholate sodium salt • Chemical Name 3 12 α Dihydroxy 5β cholan 24 oic acid monosodium salt • Molecular Weight 414 6g • Detergent Class Ionic anionic • Aggregation Number 5 average • Micelle Molecular Weight 2000g average • Critical Micelle Concentration CMC 2 to 6 mM 0 083 to 0 249 w v • Cloud Point Unknown • Dialyzable Yes Specifications for Sodium Deoxycholate Part No 89904 89905 • Formula C24H39O4Na • Molecular Weight 414 6g • Purity by HPLC 98 • Absorbance 1 Detergent Solution 340nm 0 02 280nm 0 04 260nm 0 06 • pH 1 Solution 5 to 9 • Solubility in water at 20°C 5 Sodium deoxycholate deoxycholic acid is a water soluble bile acid ionic detergent commonly used in protein methods It is most frequently used as a component of cell lysis buffers e g RIPA buffer but also has been used for liposome preparation isolation of membrane proteins and lipids preventing non specific binding in affinity chromatography and a cell culture media supplement The effectiveness of a detergent in any application depend upon the detergent s concentration Too much or too little detergent can often have a deleterious effect It is recommended that you examine a variety of detergent concentrations in your application At concentrations above 2 mM cholate will form micelles having MW 2000 The small micelle size allows easy removal by dialysis or gel filtration when needed Note removal of a detergent from a protein solution may result in protein precipitation and or aggregation Sodium deoxycholate is the detergent recommended for stripping endotoxin Lipopolysaccharide or LPS from immobilized Polymyxin B columns This is the recommended product for use with the Thermo Scientific Detoxi Gel Endotoxin Removing Gel Related Products Surfact Amps Detergent Sampler
Catalog Number:
89904
Price:
None
Category:
Lab Reagents and Chemicals
Applications:
Cell Lysis & Fractionation|Detergents for Protein Solubilization|Protein Biology|Protein Purification & Isolation
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Structured Review
Thermo Fisher
sodium deoxycholate
Thermo Scientific Sodium Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions Features of Sodium Deoxycholate • Popular anionic bile acid detergent for many laboratory uses • Effective in disrupting and dissociating many types of protein interaction • Can be removed from solution by dialysis • Useful for elution or regeneration of certain kinds of affinity columns • High purity compound with low UV absorptivity Properties of Sodium Deoxycholate • Alternative Names Sodium deoxycholic acid deoxycholate sodium salt • Chemical Name 3 12 α Dihydroxy 5β cholan 24 oic acid monosodium salt • Molecular Weight 414 6g • Detergent Class Ionic anionic • Aggregation Number 5 average • Micelle Molecular Weight 2000g average • Critical Micelle Concentration CMC 2 to 6 mM 0 083 to 0 249 w v • Cloud Point Unknown • Dialyzable Yes Specifications for Sodium Deoxycholate Part No 89904 89905 • Formula C24H39O4Na • Molecular Weight 414 6g • Purity by HPLC 98 • Absorbance 1 Detergent Solution 340nm 0 02 280nm 0 04 260nm 0 06 • pH 1 Solution 5 to 9 • Solubility in water at 20°C 5 Sodium deoxycholate deoxycholic acid is a water soluble bile acid ionic detergent commonly used in protein methods It is most frequently used as a component of cell lysis buffers e g RIPA buffer but also has been used for liposome preparation isolation of membrane proteins and lipids preventing non specific binding in affinity chromatography and a cell culture media supplement The effectiveness of a detergent in any application depend upon the detergent s concentration Too much or too little detergent can often have a deleterious effect It is recommended that you examine a variety of detergent concentrations in your application At concentrations above 2 mM cholate will form micelles having MW 2000 The small micelle size allows easy removal by dialysis or gel filtration when needed Note removal of a detergent from a protein solution may result in protein precipitation and or aggregation Sodium deoxycholate is the detergent recommended for stripping endotoxin Lipopolysaccharide or LPS from immobilized Polymyxin B columns This is the recommended product for use with the Thermo Scientific Detoxi Gel Endotoxin Removing Gel Related Products Surfact Amps Detergent Sampler
https://www.bioz.com/result/sodium deoxycholate/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Thermo Scientific Sodium Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions Features of Sodium Deoxycholate • Popular anionic bile acid detergent for many laboratory uses • Effective in disrupting and dissociating many types of protein interaction • Can be removed from solution by dialysis • Useful for elution or regeneration of certain kinds of affinity columns • High purity compound with low UV absorptivity Properties of Sodium Deoxycholate • Alternative Names Sodium deoxycholic acid deoxycholate sodium salt • Chemical Name 3 12 α Dihydroxy 5β cholan 24 oic acid monosodium salt • Molecular Weight 414 6g • Detergent Class Ionic anionic • Aggregation Number 5 average • Micelle Molecular Weight 2000g average • Critical Micelle Concentration CMC 2 to 6 mM 0 083 to 0 249 w v • Cloud Point Unknown • Dialyzable Yes Specifications for Sodium Deoxycholate Part No 89904 89905 • Formula C24H39O4Na • Molecular Weight 414 6g • Purity by HPLC 98 • Absorbance 1 Detergent Solution 340nm 0 02 280nm 0 04 260nm 0 06 • pH 1 Solution 5 to 9 • Solubility in water at 20°C 5 Sodium deoxycholate deoxycholic acid is a water soluble bile acid ionic detergent commonly used in protein methods It is most frequently used as a component of cell lysis buffers e g RIPA buffer but also has been used for liposome preparation isolation of membrane proteins and lipids preventing non specific binding in affinity chromatography and a cell culture media supplement The effectiveness of a detergent in any application depend upon the detergent s concentration Too much or too little detergent can often have a deleterious effect It is recommended that you examine a variety of detergent concentrations in your application At concentrations above 2 mM cholate will form micelles having MW 2000 The small micelle size allows easy removal by dialysis or gel filtration when needed Note removal of a detergent from a protein solution may result in protein precipitation and or aggregation Sodium deoxycholate is the detergent recommended for stripping endotoxin Lipopolysaccharide or LPS from immobilized Polymyxin B columns This is the recommended product for use with the Thermo Scientific Detoxi Gel Endotoxin Removing Gel Related Products Surfact Amps Detergent Sampler
https://www.bioz.com/result/sodium deoxycholate/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sodium deoxycholate - by Bioz Stars,
2021-03
86/100 stars
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Centrifugation:Article Title: p190RhoGAP is the convergence point of adhesion signals from ?5?1 integrin and syndecan-4 Article Snippet: For membrane fractionation by sonication, cells were scraped in Dulbecco's PBS containing calcium and magnesium (BioWhittaker) and lysed by three 5-s 20% pulses using a sonicator (Vibra-Cell; Sonics & Materials, Inc.). .. Nuclear debris was then removed with a 7 min, 1,000 g centrifugation step, and membrane-bound material was harvested at 22,000 g for a further 7 min. Harsh detergent lysates for immunoprecipitation were prepared in 50 mM Tris-Cl, pH 7.5, 150 mM NaCl, 1% (vol/vol) TrX, 0.5% (wt/vol) Immunoprecipitation:Article Title: p190RhoGAP is the convergence point of adhesion signals from ?5?1 integrin and syndecan-4 Article Snippet: For membrane fractionation by sonication, cells were scraped in Dulbecco's PBS containing calcium and magnesium (BioWhittaker) and lysed by three 5-s 20% pulses using a sonicator (Vibra-Cell; Sonics & Materials, Inc.). .. Nuclear debris was then removed with a 7 min, 1,000 g centrifugation step, and membrane-bound material was harvested at 22,000 g for a further 7 min. Harsh detergent lysates for immunoprecipitation were prepared in 50 mM Tris-Cl, pH 7.5, 150 mM NaCl, 1% (vol/vol) TrX, 0.5% (wt/vol) Affinity Purification:Article Title: p190RhoGAP is the convergence point of adhesion signals from ?5?1 integrin and syndecan-4 Article Snippet: For membrane fractionation by sonication, cells were scraped in Dulbecco's PBS containing calcium and magnesium (BioWhittaker) and lysed by three 5-s 20% pulses using a sonicator (Vibra-Cell; Sonics & Materials, Inc.). .. Nuclear debris was then removed with a 7 min, 1,000 g centrifugation step, and membrane-bound material was harvested at 22,000 g for a further 7 min. Harsh detergent lysates for immunoprecipitation were prepared in 50 mM Tris-Cl, pH 7.5, 150 mM NaCl, 1% (vol/vol) TrX, 0.5% (wt/vol) Incubation:Article Title: Modulation of Haemophilus influenzae interaction with hydrophobic molecules by the VacJ/MlaA lipoprotein impacts strongly on its interplay with the airways Article Snippet: .. Then, 100 µl of normalized suspensions were transferred to individual wells in 96-well microtiter plates (Sarstedt), to be incubated with 100 µl other:Article Title: A Novel Oral Preparation of Hydroxysafflor Yellow A Base on a Chitosan Complex: A Strategy to Enhance the Oral Bioavailability Article Snippet: |
