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Merck KGaA sodium deoxycholate
Sodium Deoxycholate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sodium deoxycholate/product/Merck KGaA
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sodium deoxycholate - by Bioz Stars, 2021-03
86/100 stars

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Protease Inhibitor:

Article Title: Single-pot, solid-phase-enhanced sample preparation for proteomics experiments.
Article Snippet: A critical step in proteomics analysis is the optimal extraction and processing of protein material to ensure the highest sensitivity in downstream detection. .. A critical step in proteomics analysis is the optimal extraction and processing of protein material to ensure the highest sensitivity in downstream detection. .. A critical step in proteomics analysis is the optimal extraction and processing of protein material to ensure the highest sensitivity in downstream detection.

Article Title: Modulation of FLT3 signal transduction through cytoplasmic cysteine residues indicates the potential for redox regulation
Article Snippet: .. To terminate treatments, tubes were transferred to ice, cells were sedimented at 500×g at 4 °C for 5 min, washed once with ice-cold PBS and subsequently lysed with 300 μl RIPA lysis buffer containing 50 mM Tris-HCl (pH7.5), 150 mM NaCl, 1% NP40 alternative (492016, Merck-Millipore, Darmstadt, Germany), 0.25% sodium deoxycholate, 1 mM EDTA, and freshly added protease inhibitor cocktail, PhosSTOP, and 1 mM sodium orthovanadate. .. The cleared lysates were subjected to immunoprecipitation with 2 μg anti-FLT3 antibody (mmc, see for reagent details) overnight, complexes were collected with protein A/G-agarose beads and analyzed by SDS-PAGE and immunoblotting, which were performed as described previously [ ].

Article Title: A hypothesis-driven approach identifies CDK4 and CDK6 inhibitors as candidate drugs for treatments of adrenocortical carcinomas
Article Snippet: Seven days (SW-13 cells) or 10 days (NCI-H295R) later, the cells were fixed with 4% para-formaldehyde for 5 min at room temperature and colored with 0.05% crystal violet (Sigma, C3886) diluted in water for 30 min. .. Western blot analysis Cells were grown on 35 mm plates (ATCC) and protein extracted with lysis buffer containing 25 mM Tris-HCl pH 7.5, 100 mM NaCl, 1 mM EDTA, 1 mM EGTA, 0.5% NP40 (#492016) (Merck Millipore), 1% Triton™ X-100 (9002-93-1, Sigma Aldrich), cOmplete™ EDTA-free Protease Inhibitor Cocktail (Sigma Aldrich), and Phosphatase Inhibitor Cocktail Set II (524625, Merck Millipore). .. Proteins were separated on NuPAGEtm 4-12% Bis-Tris gels (Thermo Fischer Scientific) and transferred onto nitrocellulose membranes (GE Healthcare).

Lysis:

Article Title: Modulation of FLT3 signal transduction through cytoplasmic cysteine residues indicates the potential for redox regulation
Article Snippet: .. To terminate treatments, tubes were transferred to ice, cells were sedimented at 500×g at 4 °C for 5 min, washed once with ice-cold PBS and subsequently lysed with 300 μl RIPA lysis buffer containing 50 mM Tris-HCl (pH7.5), 150 mM NaCl, 1% NP40 alternative (492016, Merck-Millipore, Darmstadt, Germany), 0.25% sodium deoxycholate, 1 mM EDTA, and freshly added protease inhibitor cocktail, PhosSTOP, and 1 mM sodium orthovanadate. .. The cleared lysates were subjected to immunoprecipitation with 2 μg anti-FLT3 antibody (mmc, see for reagent details) overnight, complexes were collected with protein A/G-agarose beads and analyzed by SDS-PAGE and immunoblotting, which were performed as described previously [ ].

Article Title: A hypothesis-driven approach identifies CDK4 and CDK6 inhibitors as candidate drugs for treatments of adrenocortical carcinomas
Article Snippet: Seven days (SW-13 cells) or 10 days (NCI-H295R) later, the cells were fixed with 4% para-formaldehyde for 5 min at room temperature and colored with 0.05% crystal violet (Sigma, C3886) diluted in water for 30 min. .. Western blot analysis Cells were grown on 35 mm plates (ATCC) and protein extracted with lysis buffer containing 25 mM Tris-HCl pH 7.5, 100 mM NaCl, 1 mM EDTA, 1 mM EGTA, 0.5% NP40 (#492016) (Merck Millipore), 1% Triton™ X-100 (9002-93-1, Sigma Aldrich), cOmplete™ EDTA-free Protease Inhibitor Cocktail (Sigma Aldrich), and Phosphatase Inhibitor Cocktail Set II (524625, Merck Millipore). .. Proteins were separated on NuPAGEtm 4-12% Bis-Tris gels (Thermo Fischer Scientific) and transferred onto nitrocellulose membranes (GE Healthcare).

Western Blot:

Article Title: A hypothesis-driven approach identifies CDK4 and CDK6 inhibitors as candidate drugs for treatments of adrenocortical carcinomas
Article Snippet: Seven days (SW-13 cells) or 10 days (NCI-H295R) later, the cells were fixed with 4% para-formaldehyde for 5 min at room temperature and colored with 0.05% crystal violet (Sigma, C3886) diluted in water for 30 min. .. Western blot analysis Cells were grown on 35 mm plates (ATCC) and protein extracted with lysis buffer containing 25 mM Tris-HCl pH 7.5, 100 mM NaCl, 1 mM EDTA, 1 mM EGTA, 0.5% NP40 (#492016) (Merck Millipore), 1% Triton™ X-100 (9002-93-1, Sigma Aldrich), cOmplete™ EDTA-free Protease Inhibitor Cocktail (Sigma Aldrich), and Phosphatase Inhibitor Cocktail Set II (524625, Merck Millipore). .. Proteins were separated on NuPAGEtm 4-12% Bis-Tris gels (Thermo Fischer Scientific) and transferred onto nitrocellulose membranes (GE Healthcare).

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    Merck KGaA taurodeoxycholic acid
    Bile salt hydrolase (BSH) enzyme activity assay. <t>Taurodeoxycholic</t> acid was used as the substrate for the enzyme assay, and results are therefore expressed as rate of deoxycholic acid formation (* p
    Taurodeoxycholic Acid, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taurodeoxycholic acid/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taurodeoxycholic acid - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Merck KGaA ripa buffer
    Bile salt hydrolase (BSH) enzyme activity assay. <t>Taurodeoxycholic</t> acid was used as the substrate for the enzyme assay, and results are therefore expressed as rate of deoxycholic acid formation (* p
    Ripa Buffer, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ripa buffer/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ripa buffer - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Merck KGaA deoxycholate
    Bile salt hydrolase (BSH) enzyme activity assay. <t>Taurodeoxycholic</t> acid was used as the substrate for the enzyme assay, and results are therefore expressed as rate of deoxycholic acid formation (* p
    Deoxycholate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxycholate/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxycholate - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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    Bile salt hydrolase (BSH) enzyme activity assay. Taurodeoxycholic acid was used as the substrate for the enzyme assay, and results are therefore expressed as rate of deoxycholic acid formation (* p

    Journal: Methods (San Diego, Calif.)

    Article Title: Functional Microbiomics: Evaluation of Gut Microbiota-Bile Acid Metabolism Interactions in Health and Disease

    doi: 10.1016/j.ymeth.2018.04.028

    Figure Lengend Snippet: Bile salt hydrolase (BSH) enzyme activity assay. Taurodeoxycholic acid was used as the substrate for the enzyme assay, and results are therefore expressed as rate of deoxycholic acid formation (* p

    Article Snippet: In a total volume of 200μl, 500μg of faecal protein was incubated with sodium phosphate buffer (pH 5.8, final concentration of 0.02mM), and taurodeoxycholic acid (Merck, Damstadt, Germany) (at final concentration 1mM).

    Techniques: Enzyme Activity Assay, Enzymatic Assay