Structured Review

Fisher Scientific sodium deoxycholate
Zeta potentials of docetaxel (DXT)-loaded (5 mg/mL) liposomes and chitosomes. The data are expressed as means ± SD, n = 3. CL—conventional liposome; FL—flexible liposome; T—Tween-80; DP—dicetyl phosphate; NDC—sodium <t>deoxycholate;</t> C—chitosan-coated (0.5 mg/mL).
Sodium Deoxycholate, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sodium deoxycholate/product/Fisher Scientific
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sodium deoxycholate - by Bioz Stars, 2021-03
86/100 stars

Images

1) Product Images from "Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition"

Article Title: Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition

Journal: Molecules

doi: 10.3390/molecules24020250

Zeta potentials of docetaxel (DXT)-loaded (5 mg/mL) liposomes and chitosomes. The data are expressed as means ± SD, n = 3. CL—conventional liposome; FL—flexible liposome; T—Tween-80; DP—dicetyl phosphate; NDC—sodium deoxycholate; C—chitosan-coated (0.5 mg/mL).
Figure Legend Snippet: Zeta potentials of docetaxel (DXT)-loaded (5 mg/mL) liposomes and chitosomes. The data are expressed as means ± SD, n = 3. CL—conventional liposome; FL—flexible liposome; T—Tween-80; DP—dicetyl phosphate; NDC—sodium deoxycholate; C—chitosan-coated (0.5 mg/mL).

Techniques Used:

2) Product Images from "Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition"

Article Title: Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition

Journal: Molecules

doi: 10.3390/molecules24020250

Zeta potentials of docetaxel (DXT)-loaded (5 mg/mL) liposomes and chitosomes. The data are expressed as means ± SD, n = 3. CL—conventional liposome; FL—flexible liposome; T—Tween-80; DP—dicetyl phosphate; NDC—sodium deoxycholate; C—chitosan-coated (0.5 mg/mL).
Figure Legend Snippet: Zeta potentials of docetaxel (DXT)-loaded (5 mg/mL) liposomes and chitosomes. The data are expressed as means ± SD, n = 3. CL—conventional liposome; FL—flexible liposome; T—Tween-80; DP—dicetyl phosphate; NDC—sodium deoxycholate; C—chitosan-coated (0.5 mg/mL).

Techniques Used:

3) Product Images from "Changes in Colonic Bile Acid Composition following Fecal Microbiota Transplantation Are Sufficient to Control Clostridium difficile Germination and Growth"

Article Title: Changes in Colonic Bile Acid Composition following Fecal Microbiota Transplantation Are Sufficient to Control Clostridium difficile Germination and Growth

Journal: PLoS ONE

doi: 10.1371/journal.pone.0147210

C . difficile spores do not germinate in response to secondary bile acids. A) (Left Panel) Relative OD 600 of NAP1 spores exposed to 0.5 mM (square), 1 mM (diamond), or 2 mM (triangle) DCA versus BHIS alone (circle). (Right Panel) Relative OD 600 of spores from 10 isolates after 20 min exposure to 0.5, 1, or 2 mM DCA vs. BHIS alone. B) (Left Panel) Relative OD 600 of NAP1 spores exposed to 0.5 mM (square), 1 mM (diamond), or 2 mM (triangle) LCA versus BHIS alone (circle). (Right Panel) Relative OD 600 of spores from 10 isolates after 20 min exposure to 0.5, 1, or 2 mM LCA vs. BHIS alone. OD 600 (t)/OD 600 (t 0 ) = OD 600 normalized to initial OD 600 (relative OD 600 ). Legend: n.s. = non-significant. BHIS = BHI with yeast extract and L-cysteine; DCA = deoxycholate; and LCA = lithocholic acid. Data represent mean ± SEM.
Figure Legend Snippet: C . difficile spores do not germinate in response to secondary bile acids. A) (Left Panel) Relative OD 600 of NAP1 spores exposed to 0.5 mM (square), 1 mM (diamond), or 2 mM (triangle) DCA versus BHIS alone (circle). (Right Panel) Relative OD 600 of spores from 10 isolates after 20 min exposure to 0.5, 1, or 2 mM DCA vs. BHIS alone. B) (Left Panel) Relative OD 600 of NAP1 spores exposed to 0.5 mM (square), 1 mM (diamond), or 2 mM (triangle) LCA versus BHIS alone (circle). (Right Panel) Relative OD 600 of spores from 10 isolates after 20 min exposure to 0.5, 1, or 2 mM LCA vs. BHIS alone. OD 600 (t)/OD 600 (t 0 ) = OD 600 normalized to initial OD 600 (relative OD 600 ). Legend: n.s. = non-significant. BHIS = BHI with yeast extract and L-cysteine; DCA = deoxycholate; and LCA = lithocholic acid. Data represent mean ± SEM.

Techniques Used:

Related Articles

Lysis:

Article Title: Regulation of CFTR Bicarbonate Channel Activity by WNK1: Implications for Pancreatitis and CFTR-Related Disorders
Article Snippet: .. The cells were lysed with lysis buffer (50-mM Tris-HCl, pH 7.4, 150-mM NaCl, 1% [v:v] Nonidet P-40, 0.25% [v:v] sodium deoxycholate, and a complete protease inhibitor mixture) followed by overnight incubation at 4°C with an avidin solution (UltraLink Immobilized NeutrAvidin Beads 10%; Fisher Scientific; 53150). .. Avidin-bound complexes were pelleted (15,700 g , 20 minutes) and washed 3 times with lysis buffer.

Article Title: Specific autophagy and ESCRT components participate in the unconventional secretion of CFTR
Article Snippet: Immune complexes were washed 4 times with lysis buffer prior to electrophoresis. .. For immunoblotting, cells were lysed with a lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% [v:v] Nonidet P-40, 0.25% [v:v] sodium deoxycholate [Fisher Scientific, 5285], and a complete protease inhibitor mixture). .. For biochemical analysis of LC3 and TSG101, cells were lysed with RIPA buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 1% [v:v] Nonidet P-40, 0.5% [v:v] sodium deoxycholate, 2 mM MgCl2 , 2 mM EDTA, 1% [v:v] sodium dodecyl sulfate [Sigma Aldrich, L3771], and a complete protease inhibitor mixture).

Protease Inhibitor:

Article Title: Regulation of CFTR Bicarbonate Channel Activity by WNK1: Implications for Pancreatitis and CFTR-Related Disorders
Article Snippet: .. The cells were lysed with lysis buffer (50-mM Tris-HCl, pH 7.4, 150-mM NaCl, 1% [v:v] Nonidet P-40, 0.25% [v:v] sodium deoxycholate, and a complete protease inhibitor mixture) followed by overnight incubation at 4°C with an avidin solution (UltraLink Immobilized NeutrAvidin Beads 10%; Fisher Scientific; 53150). .. Avidin-bound complexes were pelleted (15,700 g , 20 minutes) and washed 3 times with lysis buffer.

Article Title: Functional distribution of synapsin I in human sperm
Article Snippet: 2.3.1 Extraction buffer Protein extraction was conducted using chilled RIPA buffer. .. Briefly, RIPA buffer was prepared in sterile 1X PBS and contained 1% Nonidet-P40 (Fisher Scientific), 0.5% deoxycholic acid sodium salt (Fisher Scientific), 0.1% sodium dodecyl sulfate (SDS) (Thermo Scientific), and 1X protease inhibitor cocktail (Sigma–Aldrich). .. 2.3.2 Protein extracts from mouse samples All experimental procedures involving animal samples were approved by the Bloomsburg University Institutional Animal Care and Use Committee (protocol # 106).

Article Title: Specific autophagy and ESCRT components participate in the unconventional secretion of CFTR
Article Snippet: Immune complexes were washed 4 times with lysis buffer prior to electrophoresis. .. For immunoblotting, cells were lysed with a lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% [v:v] Nonidet P-40, 0.25% [v:v] sodium deoxycholate [Fisher Scientific, 5285], and a complete protease inhibitor mixture). .. For biochemical analysis of LC3 and TSG101, cells were lysed with RIPA buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 1% [v:v] Nonidet P-40, 0.5% [v:v] sodium deoxycholate, 2 mM MgCl2 , 2 mM EDTA, 1% [v:v] sodium dodecyl sulfate [Sigma Aldrich, L3771], and a complete protease inhibitor mixture).

Incubation:

Article Title: Regulation of CFTR Bicarbonate Channel Activity by WNK1: Implications for Pancreatitis and CFTR-Related Disorders
Article Snippet: .. The cells were lysed with lysis buffer (50-mM Tris-HCl, pH 7.4, 150-mM NaCl, 1% [v:v] Nonidet P-40, 0.25% [v:v] sodium deoxycholate, and a complete protease inhibitor mixture) followed by overnight incubation at 4°C with an avidin solution (UltraLink Immobilized NeutrAvidin Beads 10%; Fisher Scientific; 53150). .. Avidin-bound complexes were pelleted (15,700 g , 20 minutes) and washed 3 times with lysis buffer.

Article Title: Divergent Matrix-Remodeling Strategies Distinguish Developmental from Neoplastic Mammary Epithelial Cell Invasion Programs
Article Snippet: For LacZ staining, inguinal mammary glands and mammary tumors were fixed at room temperature for 30–45 minutes in 4% paraformaldehyde (Electron Microscopy Sciences). .. Tissues were either transferred to 30% sucrose/PBS (w/v) for frozen sections, or incubated for 18–24 hours in PBS containing 4 mM potassium hexacyanoferrate (III) (Sigma-Aldrich), 4 mM potassium hexacyanoferrate (II) trihydrate (Sigma-Aldrich), 2 mM MgCl2 (Fisher Scientific), 0.2% NP-40 (Fisher Scientific), 0.1% sodium deoxycholic acid (Fisher Scientific) and 1 mg/ml Xgal (Invitrogen). .. Stained tissues and whole mounts were post-fixed with 10% formalin-phosphate (Fisher Scientific).

Avidin-Biotin Assay:

Article Title: Regulation of CFTR Bicarbonate Channel Activity by WNK1: Implications for Pancreatitis and CFTR-Related Disorders
Article Snippet: .. The cells were lysed with lysis buffer (50-mM Tris-HCl, pH 7.4, 150-mM NaCl, 1% [v:v] Nonidet P-40, 0.25% [v:v] sodium deoxycholate, and a complete protease inhibitor mixture) followed by overnight incubation at 4°C with an avidin solution (UltraLink Immobilized NeutrAvidin Beads 10%; Fisher Scientific; 53150). .. Avidin-bound complexes were pelleted (15,700 g , 20 minutes) and washed 3 times with lysis buffer.

Transfection:

Article Title: Neuropilin-1 promotes Hedgehog signaling through a novel cytoplasmic motif
Article Snippet: COS-7 or NIH-3T3 cells were transfected using Lipofectamine 2000 (Invitrogen, 11668) and Opti-MEM reduced-serum medium (Invitrogen, 31985). .. Cells were lysed in radioimmune precipitation assay buffer (50 m m Tris-HCl (pH 7.2), 150 m m NaCl, 0.1% Triton X-100, 1% sodium deoxycholate, and 5 m m EDTA) 48 h after transfection, sonicated using a Fisher Scientific sonic dismembrator, model 500 (four pulses at 20%), and centrifuged at 14,000 × g for 25 min at 4 °C to remove the insoluble fraction. .. Protein concentrations were determined using a BCA protein assay kit (Fisher, PI23225).

Sonication:

Article Title: Neuropilin-1 promotes Hedgehog signaling through a novel cytoplasmic motif
Article Snippet: COS-7 or NIH-3T3 cells were transfected using Lipofectamine 2000 (Invitrogen, 11668) and Opti-MEM reduced-serum medium (Invitrogen, 31985). .. Cells were lysed in radioimmune precipitation assay buffer (50 m m Tris-HCl (pH 7.2), 150 m m NaCl, 0.1% Triton X-100, 1% sodium deoxycholate, and 5 m m EDTA) 48 h after transfection, sonicated using a Fisher Scientific sonic dismembrator, model 500 (four pulses at 20%), and centrifuged at 14,000 × g for 25 min at 4 °C to remove the insoluble fraction. .. Protein concentrations were determined using a BCA protein assay kit (Fisher, PI23225).

other:

Article Title: Transcriptome-wide interrogation of RNA secondary structure in living cells with icSHAPE
Article Snippet: Mix 5 g of deoxycholic acid sodium salt in 50 ml of nuclease-free water.

High Performance Liquid Chromatography:

Article Title: Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition
Article Snippet: Lipoid S100 (phosphatidylcholine (PC), soybean lecithin, > 94% PC) was purchased from Lipoid GmbH (Ludwigshafen, Germany). .. Sodium deoxycholate, methanol, and acetonitrile (HPLC grade) were purchased from Fisher Scientific Co., (Hampton, NH, USA). ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Fisher Scientific sodium deoxycholate
    Zeta potentials of docetaxel (DXT)-loaded (5 mg/mL) liposomes and chitosomes. The data are expressed as means ± SD, n = 3. CL—conventional liposome; FL—flexible liposome; T—Tween-80; DP—dicetyl phosphate; NDC—sodium <t>deoxycholate;</t> C—chitosan-coated (0.5 mg/mL).
    Sodium Deoxycholate, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium deoxycholate/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium deoxycholate - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Fisher Scientific deoxycholate sonicator
    Zeta potentials of docetaxel (DXT)-loaded (5 mg/mL) liposomes and chitosomes. The data are expressed as means ± SD, n = 3. CL—conventional liposome; FL—flexible liposome; T—Tween-80; DP—dicetyl phosphate; NDC—sodium <t>deoxycholate;</t> C—chitosan-coated (0.5 mg/mL).
    Deoxycholate Sonicator, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxycholate sonicator/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxycholate sonicator - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Zeta potentials of docetaxel (DXT)-loaded (5 mg/mL) liposomes and chitosomes. The data are expressed as means ± SD, n = 3. CL—conventional liposome; FL—flexible liposome; T—Tween-80; DP—dicetyl phosphate; NDC—sodium deoxycholate; C—chitosan-coated (0.5 mg/mL).

    Journal: Molecules

    Article Title: Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition

    doi: 10.3390/molecules24020250

    Figure Lengend Snippet: Zeta potentials of docetaxel (DXT)-loaded (5 mg/mL) liposomes and chitosomes. The data are expressed as means ± SD, n = 3. CL—conventional liposome; FL—flexible liposome; T—Tween-80; DP—dicetyl phosphate; NDC—sodium deoxycholate; C—chitosan-coated (0.5 mg/mL).

    Article Snippet: Sodium deoxycholate, methanol, and acetonitrile (HPLC grade) were purchased from Fisher Scientific Co., (Hampton, NH, USA).

    Techniques:

    Zeta potentials of docetaxel (DXT)-loaded (5 mg/mL) liposomes and chitosomes. The data are expressed as means ± SD, n = 3. CL—conventional liposome; FL—flexible liposome; T—Tween-80; DP—dicetyl phosphate; NDC—sodium deoxycholate; C—chitosan-coated (0.5 mg/mL).

    Journal: Molecules

    Article Title: Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition

    doi: 10.3390/molecules24020250

    Figure Lengend Snippet: Zeta potentials of docetaxel (DXT)-loaded (5 mg/mL) liposomes and chitosomes. The data are expressed as means ± SD, n = 3. CL—conventional liposome; FL—flexible liposome; T—Tween-80; DP—dicetyl phosphate; NDC—sodium deoxycholate; C—chitosan-coated (0.5 mg/mL).

    Article Snippet: Sodium deoxycholate, methanol, and acetonitrile (HPLC grade) were purchased from Fisher Scientific Co., (Hampton, NH, USA).

    Techniques:

    C . difficile spores do not germinate in response to secondary bile acids. A) (Left Panel) Relative OD 600 of NAP1 spores exposed to 0.5 mM (square), 1 mM (diamond), or 2 mM (triangle) DCA versus BHIS alone (circle). (Right Panel) Relative OD 600 of spores from 10 isolates after 20 min exposure to 0.5, 1, or 2 mM DCA vs. BHIS alone. B) (Left Panel) Relative OD 600 of NAP1 spores exposed to 0.5 mM (square), 1 mM (diamond), or 2 mM (triangle) LCA versus BHIS alone (circle). (Right Panel) Relative OD 600 of spores from 10 isolates after 20 min exposure to 0.5, 1, or 2 mM LCA vs. BHIS alone. OD 600 (t)/OD 600 (t 0 ) = OD 600 normalized to initial OD 600 (relative OD 600 ). Legend: n.s. = non-significant. BHIS = BHI with yeast extract and L-cysteine; DCA = deoxycholate; and LCA = lithocholic acid. Data represent mean ± SEM.

    Journal: PLoS ONE

    Article Title: Changes in Colonic Bile Acid Composition following Fecal Microbiota Transplantation Are Sufficient to Control Clostridium difficile Germination and Growth

    doi: 10.1371/journal.pone.0147210

    Figure Lengend Snippet: C . difficile spores do not germinate in response to secondary bile acids. A) (Left Panel) Relative OD 600 of NAP1 spores exposed to 0.5 mM (square), 1 mM (diamond), or 2 mM (triangle) DCA versus BHIS alone (circle). (Right Panel) Relative OD 600 of spores from 10 isolates after 20 min exposure to 0.5, 1, or 2 mM DCA vs. BHIS alone. B) (Left Panel) Relative OD 600 of NAP1 spores exposed to 0.5 mM (square), 1 mM (diamond), or 2 mM (triangle) LCA versus BHIS alone (circle). (Right Panel) Relative OD 600 of spores from 10 isolates after 20 min exposure to 0.5, 1, or 2 mM LCA vs. BHIS alone. OD 600 (t)/OD 600 (t 0 ) = OD 600 normalized to initial OD 600 (relative OD 600 ). Legend: n.s. = non-significant. BHIS = BHI with yeast extract and L-cysteine; DCA = deoxycholate; and LCA = lithocholic acid. Data represent mean ± SEM.

    Article Snippet: Reagents Sodium taurocholate, sodium cholate, sodium deoxycholate, chenodeoxycholic acid (CDCA), and lithocholic acid (LCA) were obtained from Fisher Scientific (Waltham, MA).

    Techniques: