sodium caseinate pbs  (Millipore)

 
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    Name:
    Potassium sodium phosphate buffer solution
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    40-0201
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    Structured Review

    Millipore sodium caseinate pbs
    Detection of rHbl complex formation. ( A ) Dot blot. PVDF membranes were coated with rising concentrations (3.75–480 pmol) of different rHbl components. After blocking, the membrane was incubated in <t>PBS</t> with the second component (30 pmol). Proteins were detected using the <t>Hbl</t> B-specific mAb 1B8 [ 29 ] and the Hbl L 2 -specific mAb 1H9 (this study). Inversion of the protein order showed similar results and negative controls confirmed the specificity of the reaction (see Figure S1 ). ( B ) Indirect EIA. The first rHbl component was applied as serial dilution to a microtiter plate. After washing, the second rHbl component was applied in constant concentration (60 pmol/mL). After blocking, Hbl B-specific mAb 1B8 [ 29 ] and Hbl L 2 -specific mAb 1H9 (this study) were applied, respectively, followed by rabbit-anti-mouse-HRP conjugate for detection. Details on the non-linear regression are shown in Table S2 .

    https://www.bioz.com/result/sodium caseinate pbs/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium caseinate pbs - by Bioz Stars, 2021-04
    94/100 stars

    Images

    1) Product Images from "Evidence for Complex Formation of the Bacillus cereus Haemolysin BL Components in Solution"

    Article Title: Evidence for Complex Formation of the Bacillus cereus Haemolysin BL Components in Solution

    Journal: Toxins

    doi: 10.3390/toxins9090288

    Detection of rHbl complex formation. ( A ) Dot blot. PVDF membranes were coated with rising concentrations (3.75–480 pmol) of different rHbl components. After blocking, the membrane was incubated in PBS with the second component (30 pmol). Proteins were detected using the Hbl B-specific mAb 1B8 [ 29 ] and the Hbl L 2 -specific mAb 1H9 (this study). Inversion of the protein order showed similar results and negative controls confirmed the specificity of the reaction (see Figure S1 ). ( B ) Indirect EIA. The first rHbl component was applied as serial dilution to a microtiter plate. After washing, the second rHbl component was applied in constant concentration (60 pmol/mL). After blocking, Hbl B-specific mAb 1B8 [ 29 ] and Hbl L 2 -specific mAb 1H9 (this study) were applied, respectively, followed by rabbit-anti-mouse-HRP conjugate for detection. Details on the non-linear regression are shown in Table S2 .
    Figure Legend Snippet: Detection of rHbl complex formation. ( A ) Dot blot. PVDF membranes were coated with rising concentrations (3.75–480 pmol) of different rHbl components. After blocking, the membrane was incubated in PBS with the second component (30 pmol). Proteins were detected using the Hbl B-specific mAb 1B8 [ 29 ] and the Hbl L 2 -specific mAb 1H9 (this study). Inversion of the protein order showed similar results and negative controls confirmed the specificity of the reaction (see Figure S1 ). ( B ) Indirect EIA. The first rHbl component was applied as serial dilution to a microtiter plate. After washing, the second rHbl component was applied in constant concentration (60 pmol/mL). After blocking, Hbl B-specific mAb 1B8 [ 29 ] and Hbl L 2 -specific mAb 1H9 (this study) were applied, respectively, followed by rabbit-anti-mouse-HRP conjugate for detection. Details on the non-linear regression are shown in Table S2 .

    Techniques Used: Dot Blot, Blocking Assay, Incubation, Enzyme-linked Immunosorbent Assay, Serial Dilution, Concentration Assay

    2) Product Images from "Evidence for Complex Formation of the Bacillus cereus Haemolysin BL Components in Solution"

    Article Title: Evidence for Complex Formation of the Bacillus cereus Haemolysin BL Components in Solution

    Journal: Toxins

    doi: 10.3390/toxins9090288

    Detection of rHbl complex formation. ( A ) Dot blot. PVDF membranes were coated with rising concentrations (3.75–480 pmol) of different rHbl components. After blocking, the membrane was incubated in PBS with the second component (30 pmol). Proteins were detected using the Hbl B-specific mAb 1B8 [ 29 ] and the Hbl L 2 -specific mAb 1H9 (this study). Inversion of the protein order showed similar results and negative controls confirmed the specificity of the reaction (see Figure S1 ). ( B ) Indirect EIA. The first rHbl component was applied as serial dilution to a microtiter plate. After washing, the second rHbl component was applied in constant concentration (60 pmol/mL). After blocking, Hbl B-specific mAb 1B8 [ 29 ] and Hbl L 2 -specific mAb 1H9 (this study) were applied, respectively, followed by rabbit-anti-mouse-HRP conjugate for detection. Details on the non-linear regression are shown in Table S2 .
    Figure Legend Snippet: Detection of rHbl complex formation. ( A ) Dot blot. PVDF membranes were coated with rising concentrations (3.75–480 pmol) of different rHbl components. After blocking, the membrane was incubated in PBS with the second component (30 pmol). Proteins were detected using the Hbl B-specific mAb 1B8 [ 29 ] and the Hbl L 2 -specific mAb 1H9 (this study). Inversion of the protein order showed similar results and negative controls confirmed the specificity of the reaction (see Figure S1 ). ( B ) Indirect EIA. The first rHbl component was applied as serial dilution to a microtiter plate. After washing, the second rHbl component was applied in constant concentration (60 pmol/mL). After blocking, Hbl B-specific mAb 1B8 [ 29 ] and Hbl L 2 -specific mAb 1H9 (this study) were applied, respectively, followed by rabbit-anti-mouse-HRP conjugate for detection. Details on the non-linear regression are shown in Table S2 .

    Techniques Used: Dot Blot, Blocking Assay, Incubation, Enzyme-linked Immunosorbent Assay, Serial Dilution, Concentration Assay

    Related Articles

    Concentration Assay:

    Article Title: Cytosolic acidification as a signal mediating hyperosmotic stress responses in Dictyostelium discoideum
    Article Snippet: .. Axenically grown AX-2 cells were washed twice with ice-cold SPB buffer and were resuspended in SPB buffer containing FITC-Dextran (Mw 70 kD; Sigma) at a concentration of 2 mg/ml. ..

    other:

    Article Title: Cytosolic acidification as a signal mediating hyperosmotic stress responses in Dictyostelium discoideum
    Article Snippet: To specifically acidify the cytosol, axenically grown AX-2 cells were washed twice in SPB buffer and were resuspended in SPB buffer/30 μM diethylstilbestrol (DES; Sigma).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Nitric oxide-generating compound and bio-clickable peptide mimic for synergistically tailoring surface anti-thrombogenic and anti-microbial dual-functions
    Article Snippet: .. 2.1 MaterialsCopper chloride dehydrate (CuCl2·2H2O), NaOH (Purity ≥ 98.0%), Dimethyl sulfoxide (DMSO), Tri-tert-butyl 1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetraacetate (DOTA, purity ≥ 98.0%), N-(3-dimethylaminepropyl)-N′-ethylcarbodiimide (EDC, purity ≥ 98.0%), N-hydroxysuccinimide (NHS, purity ≥ 97.0%), 2-(N-morpholino) ethanesulfonic acid hydrate (MES, Purity ≥ 97.0%), Tris-HCl (Purity ≥ 97.0%), 1-[(1-Azido-15-oxo-3,6,9,12-tetraoxapentadecan-15-yl) oxy] pyrrolidine-2,5-dione (Azido-dPEG4-NHS), polyallylamine alkaline, Acid Orange II (AO II), phosphatic buffer solution (PBS), S-nitrosoglutathione (GSNO, purity ≥ 97.0%), reduced glutathione (GSH, purity ≥ 97.0%), Cyclic Guanosine Monophosphate (cGMP) and γ-chain fibrinogen ELISA Kits were purchased from Sigma-Aldrich. .. Peptide ((DBCO-Mal)-(Mpa)-(PEG5)-WFWKWWRRRRR, DBCO-AMP) was synthesized with the assistance of China Peptides Co. Ltd. (Shanghai, China, purity > 95%).

    Sonication:

    Article Title: Local Tissue Expression of the Cell Death Ligand, FasL, Plays a Central Role in the Development of Extra-Pulmonary Acute Lung Injury
    Article Snippet: Lung MPO activity was quantified as described previously. .. [ , ] In brief, lung tissue was homogenized in 50 mM potassium buffer pH 6.0 with 0.5% hexadecyltrimethylammonium bromide (Sigma), sonicated on ice, and then centrifuged at 12,000 x g at 4°C for 10 min. Supernatants were then assayed at a 1:20 dilution in reaction buffer (530 nmol/l o -dianisidine, 150 nmol/l H2 O2 in 50 mM potassium phosphate buffer; (Sigma), and read at 450 nm. .. FasL and β -Actin were semi-quantified in lung tissue via western blotting, as previously described.

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    • sodium caseinate pbs  (Millipore)
    94
    Millipore sodium caseinate pbs
    Detection of rHbl complex formation. ( A ) Dot blot. PVDF membranes were coated with rising concentrations (3.75–480 pmol) of different rHbl components. After blocking, the membrane was incubated in <t>PBS</t> with the second component (30 pmol). Proteins were detected using the <t>Hbl</t> B-specific mAb 1B8 [ 29 ] and the Hbl L 2 -specific mAb 1H9 (this study). Inversion of the protein order showed similar results and negative controls confirmed the specificity of the reaction (see Figure S1 ). ( B ) Indirect EIA. The first rHbl component was applied as serial dilution to a microtiter plate. After washing, the second rHbl component was applied in constant concentration (60 pmol/mL). After blocking, Hbl B-specific mAb 1B8 [ 29 ] and Hbl L 2 -specific mAb 1H9 (this study) were applied, respectively, followed by rabbit-anti-mouse-HRP conjugate for detection. Details on the non-linear regression are shown in Table S2 .
    Sodium Caseinate Pbs, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium caseinate pbs/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium caseinate pbs - by Bioz Stars, 2021-04
    94/100 stars
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    mouse anti β2 m monoclonal antibody  (Millipore)
    99
    Millipore mouse anti β2 m monoclonal antibody
    Detection of rHbl complex formation. ( A ) Dot blot. PVDF membranes were coated with rising concentrations (3.75–480 pmol) of different rHbl components. After blocking, the membrane was incubated in <t>PBS</t> with the second component (30 pmol). Proteins were detected using the <t>Hbl</t> B-specific mAb 1B8 [ 29 ] and the Hbl L 2 -specific mAb 1H9 (this study). Inversion of the protein order showed similar results and negative controls confirmed the specificity of the reaction (see Figure S1 ). ( B ) Indirect EIA. The first rHbl component was applied as serial dilution to a microtiter plate. After washing, the second rHbl component was applied in constant concentration (60 pmol/mL). After blocking, Hbl B-specific mAb 1B8 [ 29 ] and Hbl L 2 -specific mAb 1H9 (this study) were applied, respectively, followed by rabbit-anti-mouse-HRP conjugate for detection. Details on the non-linear regression are shown in Table S2 .
    Mouse Anti β2 M Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti β2 m monoclonal antibody/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti β2 m monoclonal antibody - by Bioz Stars, 2021-04
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    phosphate buffered saline pbs  (Millipore)
    97
    Millipore phosphate buffered saline pbs
    Detection of rHbl complex formation. ( A ) Dot blot. PVDF membranes were coated with rising concentrations (3.75–480 pmol) of different rHbl components. After blocking, the membrane was incubated in <t>PBS</t> with the second component (30 pmol). Proteins were detected using the <t>Hbl</t> B-specific mAb 1B8 [ 29 ] and the Hbl L 2 -specific mAb 1H9 (this study). Inversion of the protein order showed similar results and negative controls confirmed the specificity of the reaction (see Figure S1 ). ( B ) Indirect EIA. The first rHbl component was applied as serial dilution to a microtiter plate. After washing, the second rHbl component was applied in constant concentration (60 pmol/mL). After blocking, Hbl B-specific mAb 1B8 [ 29 ] and Hbl L 2 -specific mAb 1H9 (this study) were applied, respectively, followed by rabbit-anti-mouse-HRP conjugate for detection. Details on the non-linear regression are shown in Table S2 .
    Phosphate Buffered Saline Pbs, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered saline pbs/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered saline pbs - by Bioz Stars, 2021-04
    97/100 stars
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    Image Search Results


    Detection of rHbl complex formation. ( A ) Dot blot. PVDF membranes were coated with rising concentrations (3.75–480 pmol) of different rHbl components. After blocking, the membrane was incubated in PBS with the second component (30 pmol). Proteins were detected using the Hbl B-specific mAb 1B8 [ 29 ] and the Hbl L 2 -specific mAb 1H9 (this study). Inversion of the protein order showed similar results and negative controls confirmed the specificity of the reaction (see Figure S1 ). ( B ) Indirect EIA. The first rHbl component was applied as serial dilution to a microtiter plate. After washing, the second rHbl component was applied in constant concentration (60 pmol/mL). After blocking, Hbl B-specific mAb 1B8 [ 29 ] and Hbl L 2 -specific mAb 1H9 (this study) were applied, respectively, followed by rabbit-anti-mouse-HRP conjugate for detection. Details on the non-linear regression are shown in Table S2 .

    Journal: Toxins

    Article Title: Evidence for Complex Formation of the Bacillus cereus Haemolysin BL Components in Solution

    doi: 10.3390/toxins9090288

    Figure Lengend Snippet: Detection of rHbl complex formation. ( A ) Dot blot. PVDF membranes were coated with rising concentrations (3.75–480 pmol) of different rHbl components. After blocking, the membrane was incubated in PBS with the second component (30 pmol). Proteins were detected using the Hbl B-specific mAb 1B8 [ 29 ] and the Hbl L 2 -specific mAb 1H9 (this study). Inversion of the protein order showed similar results and negative controls confirmed the specificity of the reaction (see Figure S1 ). ( B ) Indirect EIA. The first rHbl component was applied as serial dilution to a microtiter plate. After washing, the second rHbl component was applied in constant concentration (60 pmol/mL). After blocking, Hbl B-specific mAb 1B8 [ 29 ] and Hbl L 2 -specific mAb 1H9 (this study) were applied, respectively, followed by rabbit-anti-mouse-HRP conjugate for detection. Details on the non-linear regression are shown in Table S2 .

    Article Snippet: After that, Hbl-specific mAbs were applied (1B8 against Hbl B [ ], 1H9 against Hbl L2 (this study); 1E9 [ ] and 1G8 (this study) against Hbl L1 ; 3 μg/mL in 3% sodium-caseinate-PBS with 0.025% Tween 20) and overlaid on the dots for 1 h. The membrane was again washed for 3 × 10 min in PBS containing 0.1% Tween 20 before anti-mouse-IgG-alkaline-phosphatase-conjugate (Sigma) was applied (1:10.000 in 3% sodium-caseinate-PBS with 0.025% Tween 20) for 1 h. Again, the membrane was washed for 3 × 10 min in PBS containing 0.1% Tween 20 and for 2 × 10 min in PBS before signals were detected using NBT/BCIP solution (Roche).

    Techniques: Dot Blot, Blocking Assay, Incubation, Enzyme-linked Immunosorbent Assay, Serial Dilution, Concentration Assay