Structured Review

TaKaRa human vps29
Effects of depleting retromer subunits on the levels and gel filtration behaviors of the other subunits. (A) Triton X-100 extracts from mock-treated cells or cells treated with siRNA to Vps26, <t>Vps29,</t> Vps35, SNX1, SNX2, or SNX1/SNX2 together were subjected
Human Vps29, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human vps29/product/TaKaRa
Average 85 stars, based on 129 article reviews
Price from $9.99 to $1999.99
human vps29 - by Bioz Stars, 2020-09
85/100 stars

Images

1) Product Images from "Interchangeable but Essential Functions of SNX1 and SNX2 in the Association of Retromer with Endosomes and the Trafficking of Mannose 6-Phosphate Receptors ▿Interchangeable but Essential Functions of SNX1 and SNX2 in the Association of Retromer with Endosomes and the Trafficking of Mannose 6-Phosphate Receptors ▿ †"

Article Title: Interchangeable but Essential Functions of SNX1 and SNX2 in the Association of Retromer with Endosomes and the Trafficking of Mannose 6-Phosphate Receptors ▿Interchangeable but Essential Functions of SNX1 and SNX2 in the Association of Retromer with Endosomes and the Trafficking of Mannose 6-Phosphate Receptors ▿ †

Journal:

doi: 10.1128/MCB.00156-06

Effects of depleting retromer subunits on the levels and gel filtration behaviors of the other subunits. (A) Triton X-100 extracts from mock-treated cells or cells treated with siRNA to Vps26, Vps29, Vps35, SNX1, SNX2, or SNX1/SNX2 together were subjected
Figure Legend Snippet: Effects of depleting retromer subunits on the levels and gel filtration behaviors of the other subunits. (A) Triton X-100 extracts from mock-treated cells or cells treated with siRNA to Vps26, Vps29, Vps35, SNX1, SNX2, or SNX1/SNX2 together were subjected

Techniques Used: Filtration

Requirement of SNX1 or SNX2 for association of the Vps26-Vps29-Vps35 subcomplex with endosomal membranes. HeLa cells were treated twice, at 24-h intervals, with an inactive siRNA to Vps35 (mock; A to C) or active siRNA to Vps26 (D to F), SNX1 (G to I),
Figure Legend Snippet: Requirement of SNX1 or SNX2 for association of the Vps26-Vps29-Vps35 subcomplex with endosomal membranes. HeLa cells were treated twice, at 24-h intervals, with an inactive siRNA to Vps35 (mock; A to C) or active siRNA to Vps26 (D to F), SNX1 (G to I),

Techniques Used:

2) Product Images from "Identification of a Family of Sorting Nexin Molecules and Characterization of Their Association with Receptors"

Article Title: Identification of a Family of Sorting Nexin Molecules and Characterization of Their Association with Receptors

Journal: Molecular and Cellular Biology

doi:

Construction and characterization of SNX1, SNX1A, SNX2, SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length cDNA, the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.
Figure Legend Snippet: Construction and characterization of SNX1, SNX1A, SNX2, SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length cDNA, the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.

Techniques Used: Polymerase Chain Reaction, Construct

3) Product Images from "Interchangeable but Essential Functions of SNX1 and SNX2 in the Association of Retromer with Endosomes and the Trafficking of Mannose 6-Phosphate Receptors ▿Interchangeable but Essential Functions of SNX1 and SNX2 in the Association of Retromer with Endosomes and the Trafficking of Mannose 6-Phosphate Receptors ▿ †"

Article Title: Interchangeable but Essential Functions of SNX1 and SNX2 in the Association of Retromer with Endosomes and the Trafficking of Mannose 6-Phosphate Receptors ▿Interchangeable but Essential Functions of SNX1 and SNX2 in the Association of Retromer with Endosomes and the Trafficking of Mannose 6-Phosphate Receptors ▿ †

Journal:

doi: 10.1128/MCB.00156-06

Effects of depleting retromer subunits on the levels and gel filtration behaviors of the other subunits. (A) Triton X-100 extracts from mock-treated cells or cells treated with siRNA to Vps26, Vps29, Vps35, SNX1, SNX2, or SNX1/SNX2 together were subjected
Figure Legend Snippet: Effects of depleting retromer subunits on the levels and gel filtration behaviors of the other subunits. (A) Triton X-100 extracts from mock-treated cells or cells treated with siRNA to Vps26, Vps29, Vps35, SNX1, SNX2, or SNX1/SNX2 together were subjected

Techniques Used: Filtration

Requirement of SNX1 or SNX2 for association of the Vps26-Vps29-Vps35 subcomplex with endosomal membranes. HeLa cells were treated twice, at 24-h intervals, with an inactive siRNA to Vps35 (mock; A to C) or active siRNA to Vps26 (D to F), SNX1 (G to I),
Figure Legend Snippet: Requirement of SNX1 or SNX2 for association of the Vps26-Vps29-Vps35 subcomplex with endosomal membranes. HeLa cells were treated twice, at 24-h intervals, with an inactive siRNA to Vps35 (mock; A to C) or active siRNA to Vps26 (D to F), SNX1 (G to I),

Techniques Used:

Functional redundancy of SNX1 and SNX2 in sorting of the CI-MPR. (A to F) HeLa cells treated with siRNAs to SNX1 (A and D), SNX2 (B and E), and SNX1 and SNX2 together (C and F) were analyzed by immunofluorescence staining and confocal microscopy. Cells
Figure Legend Snippet: Functional redundancy of SNX1 and SNX2 in sorting of the CI-MPR. (A to F) HeLa cells treated with siRNAs to SNX1 (A and D), SNX2 (B and E), and SNX1 and SNX2 together (C and F) were analyzed by immunofluorescence staining and confocal microscopy. Cells

Techniques Used: Functional Assay, Immunofluorescence, Staining, Confocal Microscopy

Colocalization of endogenous SNX1, SNX2, and Vps26 in HeLa cells. The intracellular localization of SNX2 (A and D), SNX1 (B and G), and Vps26 (E and H) was assessed in fixed and permeabilized cells by indirect immunofluorescence and confocal microscopy.
Figure Legend Snippet: Colocalization of endogenous SNX1, SNX2, and Vps26 in HeLa cells. The intracellular localization of SNX2 (A and D), SNX1 (B and G), and Vps26 (E and H) was assessed in fixed and permeabilized cells by indirect immunofluorescence and confocal microscopy.

Techniques Used: Immunofluorescence, Confocal Microscopy

4) Product Images from "Identification of a Family of Sorting Nexin Molecules and Characterization of Their Association with Receptors"

Article Title: Identification of a Family of Sorting Nexin Molecules and Characterization of Their Association with Receptors

Journal: Molecular and Cellular Biology

doi:

Construction and characterization of SNX1, SNX1A, SNX2, SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length cDNA, the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.
Figure Legend Snippet: Construction and characterization of SNX1, SNX1A, SNX2, SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length cDNA, the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.

Techniques Used: Polymerase Chain Reaction, Construct

Related Articles

Clone Assay:

Article Title: The MtDMI2-MtPUB2 Negative Feedback Loop Plays a Role in Nodulation Homeostasis 1The MtDMI2-MtPUB2 Negative Feedback Loop Plays a Role in Nodulation Homeostasis 1 [OPEN]
Article Snippet: .. The sequence encoding the intracellular region of MtDMI2 was amplified by PCR and cloned into the pGBKT7 (binding domain) vector (Clontech), which was transformed into the yeast AH109 strain using the LiAc-mediated yeast transformation method ( ). .. The cDNA inserts of positive clones were amplified by PCR from yeast cells and were then sequenced.

Article Title: A Novel Role for Arabidopsis CBL1 in Affecting Plant Responses to Glucose and Gibberellin during Germination and Seedling Development
Article Snippet: .. For yeast two-hybrid assays, the PCR product was cloned into the pGBKT7 vector (Clontech) to generate the pGBKT-CBL1 bait vector. .. The full coding sequence of AKINβ1 (GenBank accession number: AT5G21170) was amplified with two primers (5′ -CAT GGATCC ATGGGAAATGCG- 3′; Bam HI site underlined, and 5′ -TGA CTCGAG CCGTGTGAGCGGTT- 3′; Xho I site underlined) and cloned into the prey vector pGADT7-Rec2 (Clontech) to generate a pGADT7-AKINβ1 vector.

Article Title: Identification of JAZ-interacting MYC transcription factors involved in latex drainage in Hevea brasiliensis
Article Snippet: .. The coding sequences of the full-length cDNAs were cloned into a pGBKT7 vector (Clontech Inc., USA), which was further transformed into a Y2HGold yeast strain. .. Transcriptional activity was examined by streaking the yeast Y2HGold transformants onto SD/-Trp/-His/-Ade/X/A media (Clontech Inc., USA).

Article Title: Microtubule-dependent endosomal sorting of clathrin-independent cargo by Hook1
Article Snippet: .. The Gal4 DNA-BD/bait construct was prepared by ligating the CD147 C-terminal PCR fragment (aa 229–269, accession no. NM_198589 ) into the BamHI–EcoRI sites of the pGBKT7 vector (TRP1; Takara Bio Inc.) using the In-Fusion Advantage PCR Cloning kit (Takara Bio Inc.). .. The yeast strain Y2HGold (Takara Bio Inc.) was maintained on YPD agar plates (rich media) or on SD –TRP (tryptophan) plates when transfected with the Gal4 DNA-BD/CD147 bait construct.

Amplification:

Article Title: The MtDMI2-MtPUB2 Negative Feedback Loop Plays a Role in Nodulation Homeostasis 1The MtDMI2-MtPUB2 Negative Feedback Loop Plays a Role in Nodulation Homeostasis 1 [OPEN]
Article Snippet: .. The sequence encoding the intracellular region of MtDMI2 was amplified by PCR and cloned into the pGBKT7 (binding domain) vector (Clontech), which was transformed into the yeast AH109 strain using the LiAc-mediated yeast transformation method ( ). .. The cDNA inserts of positive clones were amplified by PCR from yeast cells and were then sequenced.

Article Title: The selective antifungal activity of Drosophila melanogaster metchnikowin reflects the species-dependent inhibition of succinate–coenzyme Q reductase
Article Snippet: .. The PCR amplicon was digested with EcoRI and BamHI and ligated into the pGBKT7 bait vector (Takara Bio Europe/Clontech, Saint-Germain-en-Laye, France). .. The yeast bait strain Y2HGold was transformed with the bait construct and the expression of Mtk was confirmed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting.

Construct:

Article Title: Microtubule-dependent endosomal sorting of clathrin-independent cargo by Hook1
Article Snippet: .. The Gal4 DNA-BD/bait construct was prepared by ligating the CD147 C-terminal PCR fragment (aa 229–269, accession no. NM_198589 ) into the BamHI–EcoRI sites of the pGBKT7 vector (TRP1; Takara Bio Inc.) using the In-Fusion Advantage PCR Cloning kit (Takara Bio Inc.). .. The yeast strain Y2HGold (Takara Bio Inc.) was maintained on YPD agar plates (rich media) or on SD –TRP (tryptophan) plates when transfected with the Gal4 DNA-BD/CD147 bait construct.

Article Title: Regulation of Gene Transcription by Voltage-gated L-type Calcium Channel, Cav1.3 *
Article Snippet: .. A bait construct was generated in pGBKT7 vector containing a human cardiac SK2 C-terminal fragment encoding 380–487 amino acid residues (GenBankTM accession number ) ( ) and used to screen a human heart cDNA library (MATCHMAKER, Clontech) as previously described ( ). .. The construct was generated using PCR in-frame with the GAL4 BD and subcloned into the pGBKT7 vector.

Sequencing:

Article Title: The MtDMI2-MtPUB2 Negative Feedback Loop Plays a Role in Nodulation Homeostasis 1The MtDMI2-MtPUB2 Negative Feedback Loop Plays a Role in Nodulation Homeostasis 1 [OPEN]
Article Snippet: .. The sequence encoding the intracellular region of MtDMI2 was amplified by PCR and cloned into the pGBKT7 (binding domain) vector (Clontech), which was transformed into the yeast AH109 strain using the LiAc-mediated yeast transformation method ( ). .. The cDNA inserts of positive clones were amplified by PCR from yeast cells and were then sequenced.

Polymerase Chain Reaction:

Article Title: The MtDMI2-MtPUB2 Negative Feedback Loop Plays a Role in Nodulation Homeostasis 1The MtDMI2-MtPUB2 Negative Feedback Loop Plays a Role in Nodulation Homeostasis 1 [OPEN]
Article Snippet: .. The sequence encoding the intracellular region of MtDMI2 was amplified by PCR and cloned into the pGBKT7 (binding domain) vector (Clontech), which was transformed into the yeast AH109 strain using the LiAc-mediated yeast transformation method ( ). .. The cDNA inserts of positive clones were amplified by PCR from yeast cells and were then sequenced.

Article Title: A Novel Role for Arabidopsis CBL1 in Affecting Plant Responses to Glucose and Gibberellin during Germination and Seedling Development
Article Snippet: .. For yeast two-hybrid assays, the PCR product was cloned into the pGBKT7 vector (Clontech) to generate the pGBKT-CBL1 bait vector. .. The full coding sequence of AKINβ1 (GenBank accession number: AT5G21170) was amplified with two primers (5′ -CAT GGATCC ATGGGAAATGCG- 3′; Bam HI site underlined, and 5′ -TGA CTCGAG CCGTGTGAGCGGTT- 3′; Xho I site underlined) and cloned into the prey vector pGADT7-Rec2 (Clontech) to generate a pGADT7-AKINβ1 vector.

Article Title: The selective antifungal activity of Drosophila melanogaster metchnikowin reflects the species-dependent inhibition of succinate–coenzyme Q reductase
Article Snippet: .. The PCR amplicon was digested with EcoRI and BamHI and ligated into the pGBKT7 bait vector (Takara Bio Europe/Clontech, Saint-Germain-en-Laye, France). .. The yeast bait strain Y2HGold was transformed with the bait construct and the expression of Mtk was confirmed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting.

Article Title: Microtubule-dependent endosomal sorting of clathrin-independent cargo by Hook1
Article Snippet: .. The Gal4 DNA-BD/bait construct was prepared by ligating the CD147 C-terminal PCR fragment (aa 229–269, accession no. NM_198589 ) into the BamHI–EcoRI sites of the pGBKT7 vector (TRP1; Takara Bio Inc.) using the In-Fusion Advantage PCR Cloning kit (Takara Bio Inc.). .. The yeast strain Y2HGold (Takara Bio Inc.) was maintained on YPD agar plates (rich media) or on SD –TRP (tryptophan) plates when transfected with the Gal4 DNA-BD/CD147 bait construct.

Generated:

Article Title: Regulation of Gene Transcription by Voltage-gated L-type Calcium Channel, Cav1.3 *
Article Snippet: .. A bait construct was generated in pGBKT7 vector containing a human cardiac SK2 C-terminal fragment encoding 380–487 amino acid residues (GenBankTM accession number ) ( ) and used to screen a human heart cDNA library (MATCHMAKER, Clontech) as previously described ( ). .. The construct was generated using PCR in-frame with the GAL4 BD and subcloned into the pGBKT7 vector.

cDNA Library Assay:

Article Title: Regulation of Gene Transcription by Voltage-gated L-type Calcium Channel, Cav1.3 *
Article Snippet: .. A bait construct was generated in pGBKT7 vector containing a human cardiac SK2 C-terminal fragment encoding 380–487 amino acid residues (GenBankTM accession number ) ( ) and used to screen a human heart cDNA library (MATCHMAKER, Clontech) as previously described ( ). .. The construct was generated using PCR in-frame with the GAL4 BD and subcloned into the pGBKT7 vector.

Marker:

Article Title: Identification of Neuroglobin-interacting Proteins Using Yeast Two-hybrid Screening
Article Snippet: .. The vector was then transformed into yeast strain Y187 and the transformants were plated on dropout medium lacking tryptophan (SD/Trp) because the pGBKT7 vector had a selectable TRP1 marker. .. The pre-transformed Mouse Brain Matchmaker cDNA Library in pGADT7 vector was purchased from Clontech.

Transformation Assay:

Article Title: The MtDMI2-MtPUB2 Negative Feedback Loop Plays a Role in Nodulation Homeostasis 1The MtDMI2-MtPUB2 Negative Feedback Loop Plays a Role in Nodulation Homeostasis 1 [OPEN]
Article Snippet: .. The sequence encoding the intracellular region of MtDMI2 was amplified by PCR and cloned into the pGBKT7 (binding domain) vector (Clontech), which was transformed into the yeast AH109 strain using the LiAc-mediated yeast transformation method ( ). .. The cDNA inserts of positive clones were amplified by PCR from yeast cells and were then sequenced.

Article Title: Identification of Neuroglobin-interacting Proteins Using Yeast Two-hybrid Screening
Article Snippet: .. The vector was then transformed into yeast strain Y187 and the transformants were plated on dropout medium lacking tryptophan (SD/Trp) because the pGBKT7 vector had a selectable TRP1 marker. .. The pre-transformed Mouse Brain Matchmaker cDNA Library in pGADT7 vector was purchased from Clontech.

Article Title: Identification of JAZ-interacting MYC transcription factors involved in latex drainage in Hevea brasiliensis
Article Snippet: .. The coding sequences of the full-length cDNAs were cloned into a pGBKT7 vector (Clontech Inc., USA), which was further transformed into a Y2HGold yeast strain. .. Transcriptional activity was examined by streaking the yeast Y2HGold transformants onto SD/-Trp/-His/-Ade/X/A media (Clontech Inc., USA).

Binding Assay:

Article Title: The MtDMI2-MtPUB2 Negative Feedback Loop Plays a Role in Nodulation Homeostasis 1The MtDMI2-MtPUB2 Negative Feedback Loop Plays a Role in Nodulation Homeostasis 1 [OPEN]
Article Snippet: .. The sequence encoding the intracellular region of MtDMI2 was amplified by PCR and cloned into the pGBKT7 (binding domain) vector (Clontech), which was transformed into the yeast AH109 strain using the LiAc-mediated yeast transformation method ( ). .. The cDNA inserts of positive clones were amplified by PCR from yeast cells and were then sequenced.

Plasmid Preparation:

Article Title: The MtDMI2-MtPUB2 Negative Feedback Loop Plays a Role in Nodulation Homeostasis 1The MtDMI2-MtPUB2 Negative Feedback Loop Plays a Role in Nodulation Homeostasis 1 [OPEN]
Article Snippet: .. The sequence encoding the intracellular region of MtDMI2 was amplified by PCR and cloned into the pGBKT7 (binding domain) vector (Clontech), which was transformed into the yeast AH109 strain using the LiAc-mediated yeast transformation method ( ). .. The cDNA inserts of positive clones were amplified by PCR from yeast cells and were then sequenced.

Article Title: Identification of Neuroglobin-interacting Proteins Using Yeast Two-hybrid Screening
Article Snippet: .. The vector was then transformed into yeast strain Y187 and the transformants were plated on dropout medium lacking tryptophan (SD/Trp) because the pGBKT7 vector had a selectable TRP1 marker. .. The pre-transformed Mouse Brain Matchmaker cDNA Library in pGADT7 vector was purchased from Clontech.

Article Title: A Novel Role for Arabidopsis CBL1 in Affecting Plant Responses to Glucose and Gibberellin during Germination and Seedling Development
Article Snippet: .. For yeast two-hybrid assays, the PCR product was cloned into the pGBKT7 vector (Clontech) to generate the pGBKT-CBL1 bait vector. .. The full coding sequence of AKINβ1 (GenBank accession number: AT5G21170) was amplified with two primers (5′ -CAT GGATCC ATGGGAAATGCG- 3′; Bam HI site underlined, and 5′ -TGA CTCGAG CCGTGTGAGCGGTT- 3′; Xho I site underlined) and cloned into the prey vector pGADT7-Rec2 (Clontech) to generate a pGADT7-AKINβ1 vector.

Article Title: Identification of JAZ-interacting MYC transcription factors involved in latex drainage in Hevea brasiliensis
Article Snippet: .. The coding sequences of the full-length cDNAs were cloned into a pGBKT7 vector (Clontech Inc., USA), which was further transformed into a Y2HGold yeast strain. .. Transcriptional activity was examined by streaking the yeast Y2HGold transformants onto SD/-Trp/-His/-Ade/X/A media (Clontech Inc., USA).

Article Title: The selective antifungal activity of Drosophila melanogaster metchnikowin reflects the species-dependent inhibition of succinate–coenzyme Q reductase
Article Snippet: .. The PCR amplicon was digested with EcoRI and BamHI and ligated into the pGBKT7 bait vector (Takara Bio Europe/Clontech, Saint-Germain-en-Laye, France). .. The yeast bait strain Y2HGold was transformed with the bait construct and the expression of Mtk was confirmed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting.

Article Title: Identification of a Novel SBP1-Containing SCFSFB Complex in Wild Dwarf Almond (Prunus tenella)
Article Snippet: .. The pGADT7 vector (Clontech, CA, USA) was digested by Bam H I and Xho I (Takara, Dalian, China), while the pGBKT7 vector (Clontech) was digested by Bam H I and Pst I (Takara). .. Then enzyme-digested vectors were run on 1.0% agarose gel and purified by two 15-min phenol–chloroform (Solarbio, Peking, China) treatments at 4°C.

Article Title: Microtubule-dependent endosomal sorting of clathrin-independent cargo by Hook1
Article Snippet: .. The Gal4 DNA-BD/bait construct was prepared by ligating the CD147 C-terminal PCR fragment (aa 229–269, accession no. NM_198589 ) into the BamHI–EcoRI sites of the pGBKT7 vector (TRP1; Takara Bio Inc.) using the In-Fusion Advantage PCR Cloning kit (Takara Bio Inc.). .. The yeast strain Y2HGold (Takara Bio Inc.) was maintained on YPD agar plates (rich media) or on SD –TRP (tryptophan) plates when transfected with the Gal4 DNA-BD/CD147 bait construct.

Article Title: Regulation of Gene Transcription by Voltage-gated L-type Calcium Channel, Cav1.3 *
Article Snippet: .. A bait construct was generated in pGBKT7 vector containing a human cardiac SK2 C-terminal fragment encoding 380–487 amino acid residues (GenBankTM accession number ) ( ) and used to screen a human heart cDNA library (MATCHMAKER, Clontech) as previously described ( ). .. The construct was generated using PCR in-frame with the GAL4 BD and subcloned into the pGBKT7 vector.

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    TaKaRa snx2 cdna
    Construction and characterization of SNX1, SNX1A, <t>SNX2,</t> SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length <t>cDNA,</t> the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.
    Snx2 Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snx2 cdna/product/TaKaRa
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snx2 cdna - by Bioz Stars, 2020-09
    80/100 stars
      Buy from Supplier

    Image Search Results


    Construction and characterization of SNX1, SNX1A, SNX2, SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length cDNA, the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.

    Journal: Molecular and Cellular Biology

    Article Title: Identification of a Family of Sorting Nexin Molecules and Characterization of Their Association with Receptors

    doi:

    Figure Lengend Snippet: Construction and characterization of SNX1, SNX1A, SNX2, SNX3, and SNX4 cDNAs. Five human sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are depicted. For each full-length cDNA, the nucleotide numbering is shown. Listed below each molecule are the fragments (ESTs, PCR, or 5′-RACE products) used to determine the nucleotide sequences of the cDNAs and to construct full-length cDNAs as detailed in Materials and Methods. One or both strands of each cDNA fragment were sequenced as indicated by the directions of the arrowheads. In every case, both strands of DNA were sequenced from at least one clone.

    Article Snippet: A 5′-rapid amplification of cDNA ends (RACE) strategy (Life Technologies, Inc., Gaithersburg, Md.) with total human skeletal muscle as a template was used to complete the 5′ end of the SNX2 cDNA (Clontech).

    Techniques: Polymerase Chain Reaction, Construct