snap cell 505 star  (New England Biolabs)


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    Name:
    SNAP Cell 505 Star
    Description:
    SNAP Cell 505 Star 50 nmol
    Catalog Number:
    S9103S
    Price:
    344
    Category:
    Fluorochromes
    Size:
    50 nmol
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    Structured Review

    New England Biolabs snap cell 505 star
    SNAP Cell 505 Star
    SNAP Cell 505 Star 50 nmol
    https://www.bioz.com/result/snap cell 505 star/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap cell 505 star - by Bioz Stars, 2021-05
    92/100 stars

    Images

    1) Product Images from "Engineering a living biomaterial via bacterial surface capture of environmental molecules"

    Article Title: Engineering a living biomaterial via bacterial surface capture of environmental molecules

    Journal: Synthetic Biology

    doi: 10.1093/synbio/ysy017

    Examination of the functionality of SNAP was determined by using a fluorescent label modified with a BG group (SNAP-Cell ® 505-Star). ( a ) Schematic of surface-display system and ( b ) fluorescent label addition. ( c ) Histogram and ( d ) bar graph from flow cytometry analysis showing the shift in fluorescence of cells labelled after growth with and without inducer. Tests were performed in triplicate with 10 000 events per sample (* P
    Figure Legend Snippet: Examination of the functionality of SNAP was determined by using a fluorescent label modified with a BG group (SNAP-Cell ® 505-Star). ( a ) Schematic of surface-display system and ( b ) fluorescent label addition. ( c ) Histogram and ( d ) bar graph from flow cytometry analysis showing the shift in fluorescence of cells labelled after growth with and without inducer. Tests were performed in triplicate with 10 000 events per sample (* P

    Techniques Used: Modification, Flow Cytometry, Fluorescence

    2) Product Images from "BRK phosphorylates SMAD4 for proteasomal degradation and inhibits tumor suppressor FRK to control SNAIL, SLUG, and metastatic potential"

    Article Title: BRK phosphorylates SMAD4 for proteasomal degradation and inhibits tumor suppressor FRK to control SNAIL, SLUG, and metastatic potential

    Journal: Science Advances

    doi: 10.1126/sciadv.aaw3113

    Ectopically expressed BRK and SMAD4 interact and colocalize in HEK293 cells. ( A and B ) SNAP-FLAG-BRK-YF (SF-BRK-YF) and HALO-SMAD2/3/4 were expressed into HEK293 cells, and cell lysates were subjected to affinity purification (AP) with Halo magnetic beads (A) or SNAP capture magnetic beads (B) antibodies, followed by immunoblotting using anti-Halo and anti-FLAG antibodies. Bottom: The ectopic expression of BRK and SMAD2/SMAD3/SMAD4 as detected by anti-Halo and anti-FLAG antibodies. During affinity purification, either the SNAP_Flag or Halo tags were clipped off using Precision or Tev proteases, respectively. Halo-SMAD4 is ~93 kDa; after Halo-Tag removal, SMAD4 is ~60 kDa. Similarly, SNAP-Flag-BRK is ~73 kDa, and after SNAP-Tag removal, BRK is ~50 kDa. In (B), the three different blots in the middle were probed with the SMAD2, SMAD3, and SMAD4 antibodies. ( C ) HEK293 cells were cotransfected with SF-BRK-YF, Halo-SMAD2, Halo-SMAD3, and Halo-SMAD4, and the cell lysates were subjected to affinity purification with Halo magnetic beads or SNAP capture magnetic beads, followed by immunoblotting using anti-SMAD2, anti-SMAD3, anti-SMAD4, and anti-Flag antibodies. Total cell lysates were also analyzed by immunoblotting using antibodies against Halo-SMAD2, Halo-SMAD3, Halo-SMAD4, and Flag. ( D ) GFP-SMAD4 was cotransfected with BRK-W44A, BRK-∆SH2, BRK-∆SH3, BRK-WT, BRK-Y342F, or BRK-YF. The corresponding protein extracts were immunoprecipitated with anti-GFP and mouse immunoglobulin G (IgG; lysates from SMAD4 and BRK-YF cotransfected cells as a representative) and immunoblotted with anti-BRK and anti-GFP antibodies and β-actin as a loading control. IP, immunoprecipitation. ( E ) SF-BRK-YF was expressed either alone or with Halo-SMAD4 [full length (FL)] or SMAD4 deletion mutants (A to E) in HEK293 cells. Total cell lysates were subjected to Halo affinity purification and analyzed by immunoblotting with FLAG and Halo antibodies. ( F ) Halo-SMAD4 or Halo plasmid alone with SF-BRK-WT or SF-BRK-YF was ectopically expressed in HEK293T cells. SNAP affinity purification followed by MudPIT mass spectrometry analysis showed that Halo-SMAD4 copurified with SNAP-Flag-BRK-WT or SNAP-Flag-BRK-YF but not with Halo alone. ( G ) Halo-SMAD4 or SF-BRK-YF alone or in combination was transfected into HEK293T cells. Halo-Tag TMRDirect fluorescent ligand (red) and SNAP-Cell 505-Star (green) were used to label Halo-Tag and SNAP-Tag proteins, respectively; DNA was stained with Hoechst dye (blue). DAPI, 4′,6-diamidino-2-phenylindole.
    Figure Legend Snippet: Ectopically expressed BRK and SMAD4 interact and colocalize in HEK293 cells. ( A and B ) SNAP-FLAG-BRK-YF (SF-BRK-YF) and HALO-SMAD2/3/4 were expressed into HEK293 cells, and cell lysates were subjected to affinity purification (AP) with Halo magnetic beads (A) or SNAP capture magnetic beads (B) antibodies, followed by immunoblotting using anti-Halo and anti-FLAG antibodies. Bottom: The ectopic expression of BRK and SMAD2/SMAD3/SMAD4 as detected by anti-Halo and anti-FLAG antibodies. During affinity purification, either the SNAP_Flag or Halo tags were clipped off using Precision or Tev proteases, respectively. Halo-SMAD4 is ~93 kDa; after Halo-Tag removal, SMAD4 is ~60 kDa. Similarly, SNAP-Flag-BRK is ~73 kDa, and after SNAP-Tag removal, BRK is ~50 kDa. In (B), the three different blots in the middle were probed with the SMAD2, SMAD3, and SMAD4 antibodies. ( C ) HEK293 cells were cotransfected with SF-BRK-YF, Halo-SMAD2, Halo-SMAD3, and Halo-SMAD4, and the cell lysates were subjected to affinity purification with Halo magnetic beads or SNAP capture magnetic beads, followed by immunoblotting using anti-SMAD2, anti-SMAD3, anti-SMAD4, and anti-Flag antibodies. Total cell lysates were also analyzed by immunoblotting using antibodies against Halo-SMAD2, Halo-SMAD3, Halo-SMAD4, and Flag. ( D ) GFP-SMAD4 was cotransfected with BRK-W44A, BRK-∆SH2, BRK-∆SH3, BRK-WT, BRK-Y342F, or BRK-YF. The corresponding protein extracts were immunoprecipitated with anti-GFP and mouse immunoglobulin G (IgG; lysates from SMAD4 and BRK-YF cotransfected cells as a representative) and immunoblotted with anti-BRK and anti-GFP antibodies and β-actin as a loading control. IP, immunoprecipitation. ( E ) SF-BRK-YF was expressed either alone or with Halo-SMAD4 [full length (FL)] or SMAD4 deletion mutants (A to E) in HEK293 cells. Total cell lysates were subjected to Halo affinity purification and analyzed by immunoblotting with FLAG and Halo antibodies. ( F ) Halo-SMAD4 or Halo plasmid alone with SF-BRK-WT or SF-BRK-YF was ectopically expressed in HEK293T cells. SNAP affinity purification followed by MudPIT mass spectrometry analysis showed that Halo-SMAD4 copurified with SNAP-Flag-BRK-WT or SNAP-Flag-BRK-YF but not with Halo alone. ( G ) Halo-SMAD4 or SF-BRK-YF alone or in combination was transfected into HEK293T cells. Halo-Tag TMRDirect fluorescent ligand (red) and SNAP-Cell 505-Star (green) were used to label Halo-Tag and SNAP-Tag proteins, respectively; DNA was stained with Hoechst dye (blue). DAPI, 4′,6-diamidino-2-phenylindole.

    Techniques Used: Affinity Purification, Magnetic Beads, Expressing, Immunoprecipitation, Plasmid Preparation, Mass Spectrometry, Transfection, Staining

    3) Product Images from "Engineering a living biomaterial via bacterial surface capture of environmental molecules"

    Article Title: Engineering a living biomaterial via bacterial surface capture of environmental molecules

    Journal: Synthetic Biology

    doi: 10.1093/synbio/ysy017

    Examination of the functionality of SNAP was determined by using a fluorescent label modified with a BG group (SNAP-Cell ® 505-Star). ( a ) Schematic of surface-display system and ( b ) fluorescent label addition. ( c ) Histogram and ( d ) bar graph from flow cytometry analysis showing the shift in fluorescence of cells labelled after growth with and without inducer. Tests were performed in triplicate with 10 000 events per sample (* P
    Figure Legend Snippet: Examination of the functionality of SNAP was determined by using a fluorescent label modified with a BG group (SNAP-Cell ® 505-Star). ( a ) Schematic of surface-display system and ( b ) fluorescent label addition. ( c ) Histogram and ( d ) bar graph from flow cytometry analysis showing the shift in fluorescence of cells labelled after growth with and without inducer. Tests were performed in triplicate with 10 000 events per sample (* P

    Techniques Used: Modification, Flow Cytometry, Fluorescence

    Related Articles

    Binding Assay:

    Article Title: Engineering a living biomaterial via bacterial surface capture of environmental molecules
    Article Snippet: 2.4 Fluorescence labelling SNAP-Cell® 505-Star is a fluorescent label purchased from New England Biolabs, Inc., with an excitation peak at 504 nm and an emission peak at 532 nm. .. SNAP-Cell® 505-Star was an ideal candidate for tests because the label excitation and emission spectra did not result in significant cross-talk with mCherry and the label binding was restricted to the surface of the cells, eliminating any chance of labeling cytosolic proteins. .. The following procedures were all based on New England Biolabs, Inc.’s Cellular Labelling (S9103) protocol.

    Labeling:

    Article Title: Engineering a living biomaterial via bacterial surface capture of environmental molecules
    Article Snippet: 2.4 Fluorescence labelling SNAP-Cell® 505-Star is a fluorescent label purchased from New England Biolabs, Inc., with an excitation peak at 504 nm and an emission peak at 532 nm. .. SNAP-Cell® 505-Star was an ideal candidate for tests because the label excitation and emission spectra did not result in significant cross-talk with mCherry and the label binding was restricted to the surface of the cells, eliminating any chance of labeling cytosolic proteins. .. The following procedures were all based on New England Biolabs, Inc.’s Cellular Labelling (S9103) protocol.

    Article Title: Histone Methylation by SETD1A Protects Nascent DNA through the Nucleosome Chaperone Activity of FANCD2
    Article Snippet: U-2-OS cells were grown on glass coverslips, transfected with siRNA, incubated for 48 hr, and transfected with pSNAPm-H3.1 using FuGene HD. .. 24 hr post DNA transfection, pre-existing SNAP-H3.1 was labeled with SNAP-cell 505 star (New England Biolabs) according to the manufacturer’s instructions (‘pulse’). .. Alternatively, pre-existing H3.1 was quenched by incubating cells with SNAP-cell Block (New England Biolabs), and then cells were pulsed as described above (‘quench-pulse’), or released into 2 mM HU for 2 hr before newly synthesized SNAP-H3.1 was labeled as above (‘quench-HU-pulse’).

    Article Title: BRK phosphorylates SMAD4 for proteasomal degradation and inhibits tumor suppressor FRK to control SNAIL, SLUG, and metastatic potential
    Article Snippet: Live-cell imaging HEK293T cells were seeded onto glass-bottom culture dishes (MatTek, Ashland, MA) and transiently transfected with the SNAP-Flag-BRK and Halo-SMAD4 constructs. .. Affinity-tagged proteins were fluorescently labeled during growth, either with Halo-Tag TMRDirect ligand (Promega) or SNAP-Cell 505-Star (NEB) or with both ligands according to the manufacturer’s instructions. .. Images were taken with a ZEISS LSM 780 confocal microscope with argon laser excitation at 573 to 687 nm for TMRDirect and 499 to 526 nm for SNAP-Cell 505-Star.

    Transfection:

    Article Title: Histone Methylation by SETD1A Protects Nascent DNA through the Nucleosome Chaperone Activity of FANCD2
    Article Snippet: U-2-OS cells were grown on glass coverslips, transfected with siRNA, incubated for 48 hr, and transfected with pSNAPm-H3.1 using FuGene HD. .. 24 hr post DNA transfection, pre-existing SNAP-H3.1 was labeled with SNAP-cell 505 star (New England Biolabs) according to the manufacturer’s instructions (‘pulse’). .. Alternatively, pre-existing H3.1 was quenched by incubating cells with SNAP-cell Block (New England Biolabs), and then cells were pulsed as described above (‘quench-pulse’), or released into 2 mM HU for 2 hr before newly synthesized SNAP-H3.1 was labeled as above (‘quench-HU-pulse’).

    Fluorescence:

    Article Title: Engineering a living biomaterial via bacterial surface capture of environmental molecules
    Article Snippet: A 5 min incubation at 95°C followed by 10 min at −80°C were added in front of the touchdown PCR program for whole-cell PCR. .. 2.4 Fluorescence labelling SNAP-Cell® 505-Star is a fluorescent label purchased from New England Biolabs, Inc., with an excitation peak at 504 nm and an emission peak at 532 nm. .. SNAP-Cell® 505-Star was an ideal candidate for tests because the label excitation and emission spectra did not result in significant cross-talk with mCherry and the label binding was restricted to the surface of the cells, eliminating any chance of labeling cytosolic proteins.

    Incubation:

    Article Title: Referenced Single-Molecule Measurements Differentiate between GPCR Oligomerization States
    Article Snippet: A stock solution of HaloTag TMR Ligand (Promega, Madison, WI) was diluted in supplemented DMEM to a final concentration of 5 nM. .. After incubation posttransfection, all medium was removed from the cells and replaced with supplemented DMEM containing 5 nM HaloTag TMR Ligand (Promega) and 5 μ M SNAP-Cell 505-Star (New England Biolabs, Ipswich, MA) before incubation for 30 min at 37°C. .. Microscope coverslips were plasma cleaned (PDC-002, Harrick Plasma, Ithaca, NY) in an argon atmosphere for 30 min before subsequent coating with poly-L-lysine-grafted polyethyleneglycol (PLL-g-PEG, SuSoS) for 45 min.

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    New England Biolabs snap cell 505 star
    Examination of the functionality of SNAP was determined by using a fluorescent label modified with a BG group <t>(SNAP-Cell</t> ® 505-Star). ( a ) Schematic of surface-display system and ( b ) fluorescent label addition. ( c ) Histogram and ( d ) bar graph from flow cytometry analysis showing the shift in fluorescence of cells labelled after growth with and without inducer. Tests were performed in triplicate with 10 000 events per sample (* P
    Snap Cell 505 Star, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap cell 505 star/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap cell 505 star - by Bioz Stars, 2021-05
    92/100 stars
      Buy from Supplier

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    Examination of the functionality of SNAP was determined by using a fluorescent label modified with a BG group (SNAP-Cell ® 505-Star). ( a ) Schematic of surface-display system and ( b ) fluorescent label addition. ( c ) Histogram and ( d ) bar graph from flow cytometry analysis showing the shift in fluorescence of cells labelled after growth with and without inducer. Tests were performed in triplicate with 10 000 events per sample (* P

    Journal: Synthetic Biology

    Article Title: Engineering a living biomaterial via bacterial surface capture of environmental molecules

    doi: 10.1093/synbio/ysy017

    Figure Lengend Snippet: Examination of the functionality of SNAP was determined by using a fluorescent label modified with a BG group (SNAP-Cell ® 505-Star). ( a ) Schematic of surface-display system and ( b ) fluorescent label addition. ( c ) Histogram and ( d ) bar graph from flow cytometry analysis showing the shift in fluorescence of cells labelled after growth with and without inducer. Tests were performed in triplicate with 10 000 events per sample (* P

    Article Snippet: SNAP-Cell® 505-Star was an ideal candidate for tests because the label excitation and emission spectra did not result in significant cross-talk with mCherry and the label binding was restricted to the surface of the cells, eliminating any chance of labeling cytosolic proteins.

    Techniques: Modification, Flow Cytometry, Fluorescence

    HDAC1 associates with components of the prefoldin-mediated CCT folding pathway. ( A used to model possible HDAC1 processing by the prefoldin/CCT pathway in the nucleus. ( B . ( C ) VBP1 colocalises with HDAC1 in the nucleus. Flp-In™-293 host cells stably expressing Halo-HDAC1 were transiently transfected with SNAP-FLAG-VBP1 and proteins imaged using HaloTag® TMRDirect™ ligand (Halo-HDAC1; red), SNAP-Cell® 505-Star ligand (SNAP-FLAG-VBP1; green), and Hoechst dye (nuclei; blue). ( D network showing established TCP1 (CCT complex) interacting proteins was generated using the following settings: minimum required interaction score - medium confidence (0.4); maximum number of interactors - 50; active interaction source – Experiments; clustering method – kmeans (default settings). Clusters are indicated by node color.

    Journal: Scientific Reports

    Article Title: Differential HDAC1/2 network analysis reveals a role for prefoldin/CCT in HDAC1/2 complex assembly

    doi: 10.1038/s41598-018-32009-w

    Figure Lengend Snippet: HDAC1 associates with components of the prefoldin-mediated CCT folding pathway. ( A used to model possible HDAC1 processing by the prefoldin/CCT pathway in the nucleus. ( B . ( C ) VBP1 colocalises with HDAC1 in the nucleus. Flp-In™-293 host cells stably expressing Halo-HDAC1 were transiently transfected with SNAP-FLAG-VBP1 and proteins imaged using HaloTag® TMRDirect™ ligand (Halo-HDAC1; red), SNAP-Cell® 505-Star ligand (SNAP-FLAG-VBP1; green), and Hoechst dye (nuclei; blue). ( D network showing established TCP1 (CCT complex) interacting proteins was generated using the following settings: minimum required interaction score - medium confidence (0.4); maximum number of interactors - 50; active interaction source – Experiments; clustering method – kmeans (default settings). Clusters are indicated by node color.

    Article Snippet: SNAP-tagged VBP1 was similarly imaged using SNAP-Cell® 505-Star ligand (NEB) according to the manufacturer’s instructions.

    Techniques: Stable Transfection, Expressing, Transfection, Generated