sambucus nigra agglutinin sna  (Vector Laboratories)


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    Vector Laboratories sambucus nigra agglutinin sna
    Sambucus Nigra Agglutinin Sna, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sambucus nigra agglutinin sna  (Vector Laboratories)


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    Vector Laboratories sambucus nigra agglutinin sna
    Sambucus Nigra Agglutinin Sna, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sambucus nigra agglutinin sna/product/Vector Laboratories
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    plant lectins sna  (Vector Laboratories)


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    Vector Laboratories plant lectins sna
    The binding to mammary glands of three different cows was investigated for different IAV H5 proteins and a human H1 protein, as well as an IDV HEF and plant <t>lectins</t> MAL-II and <t>SNA.</t> AEC staining was used to visualize tissue binding.
    Plant Lectins Sna, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The mammary glands of cows abundantly display receptors for circulating avian H5 viruses"

    Article Title: The mammary glands of cows abundantly display receptors for circulating avian H5 viruses

    Journal: bioRxiv

    doi: 10.1101/2024.05.24.595667

    The binding to mammary glands of three different cows was investigated for different IAV H5 proteins and a human H1 protein, as well as an IDV HEF and plant lectins MAL-II and SNA. AEC staining was used to visualize tissue binding.
    Figure Legend Snippet: The binding to mammary glands of three different cows was investigated for different IAV H5 proteins and a human H1 protein, as well as an IDV HEF and plant lectins MAL-II and SNA. AEC staining was used to visualize tissue binding.

    Techniques Used: Binding Assay, Staining

    Hemagglutination assay with chicken erythrocytes with the lectins used in this study.
    Figure Legend Snippet: Hemagglutination assay with chicken erythrocytes with the lectins used in this study.

    Techniques Used: Hemagglutination Assay

    sna  (Vector Laboratories)


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    Vector Laboratories sna
    (A) HEK293S GnTI-derived HKU1 NTD Fc and HKU1 NTD Fc + pA-LS displayed increased binding to BSM in comparison to their 293T-derived equivalents. (B) Presence of α2-3-SIA on BSM was confirmed by <t>utilizing</t> <t>MAL-II,</t> while <t>SNA</t> binding confirmed the presence of α2-6-SIA. Trimeric HKU1 NTD (GnTI- and 293-derived), pA-LS NP, and antibody control did not bind BSM.
    Sna, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The HCoV-HKU1 N-terminal domain binds a wide range of 9- O -acetylated sialic acids presented on different glycan cores"

    Article Title: The HCoV-HKU1 N-terminal domain binds a wide range of 9- O -acetylated sialic acids presented on different glycan cores

    Journal: bioRxiv

    doi: 10.1101/2024.05.24.595699

    (A) HEK293S GnTI-derived HKU1 NTD Fc and HKU1 NTD Fc + pA-LS displayed increased binding to BSM in comparison to their 293T-derived equivalents. (B) Presence of α2-3-SIA on BSM was confirmed by utilizing MAL-II, while SNA binding confirmed the presence of α2-6-SIA. Trimeric HKU1 NTD (GnTI- and 293-derived), pA-LS NP, and antibody control did not bind BSM.
    Figure Legend Snippet: (A) HEK293S GnTI-derived HKU1 NTD Fc and HKU1 NTD Fc + pA-LS displayed increased binding to BSM in comparison to their 293T-derived equivalents. (B) Presence of α2-3-SIA on BSM was confirmed by utilizing MAL-II, while SNA binding confirmed the presence of α2-6-SIA. Trimeric HKU1 NTD (GnTI- and 293-derived), pA-LS NP, and antibody control did not bind BSM.

    Techniques Used: Derivative Assay, Binding Assay, Comparison

    fitc conjugated sna lectin  (Vector Laboratories)


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    Vector Laboratories fitc conjugated sna lectin
    Sialic acid blockade can prevent/inhibit prostate cancer bone metastasis. ( a ) Inhibition of sialylation in TRAMPC2 cells using P-SiaFNEtoc detected using pan-specific Lectenz <t>lectin</t> flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( b ) Detection of α2-6 linked sialylated N -glycans in TRAMPC2 cells using <t>SNA</t> lectin flow cytometry. TRAMPC2 cells treated with 64 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p = 0.0001). ( c ) Luciferase tagged TRAMPC2 cells (control or pre-treated with 64 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via sub-cutaneous injection and tumours were monitored using in vivo bioluminescence imaging. Pre-treatment of TRAMPC2 cells with P-SiaFNEtoc (which removed sialylated glycans) significantly reduced tumour burden over 6 weeks (n = 10, Mann–Whitney test, p = 0.0233) thus suggesting that sialic acid blockade has the potential to inhibit the growth of prostate tumours. ( d ) Inhibition of sialylation in RM1 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( e ) Detection of α2-6 linked sialylated N -glycans in RM1 cells using SNA lectin flow cytometry. RM1 cells treated with 256 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p < 0.0001). ( f ) Luciferase tagged RM1 cells (control or pre-treated with 256 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via intra cardiac injection. Tumours were monitored over 15 days using in vivo bioluminescence imaging. ( g , h ) Pre-treatment of RM1 cells with P-SiaFNEtoc (to remove sialylated glycans) significantly reduced the number of skeletal tumours formed (Mann–Whitney test, p = 0.0454), the incidence of tumour in left tibias (Chi-square test, p = 0.0455), and significantly increased survival time in mice (Log-rank test, p = 0.012). ( i ) Micro-CT analysis demonstrated that P-SiaFNEtoc significantly alleviated bone destruction in the trabecular bone of tibias and increased trabecular bone volume (BV/TV, p = 0.0211) and trabecular number (Tb. N, p = 0.035) (n = 9, unpaired t test, ∗p < 0.05). Representative images are shown. Scale bar is 200 μm.
    Fitc Conjugated Sna Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Sialic acid blockade inhibits the metastatic spread of prostate cancer to bone"

    Article Title: Sialic acid blockade inhibits the metastatic spread of prostate cancer to bone

    Journal: eBioMedicine

    doi: 10.1016/j.ebiom.2024.105163

    Sialic acid blockade can prevent/inhibit prostate cancer bone metastasis. ( a ) Inhibition of sialylation in TRAMPC2 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( b ) Detection of α2-6 linked sialylated N -glycans in TRAMPC2 cells using SNA lectin flow cytometry. TRAMPC2 cells treated with 64 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p = 0.0001). ( c ) Luciferase tagged TRAMPC2 cells (control or pre-treated with 64 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via sub-cutaneous injection and tumours were monitored using in vivo bioluminescence imaging. Pre-treatment of TRAMPC2 cells with P-SiaFNEtoc (which removed sialylated glycans) significantly reduced tumour burden over 6 weeks (n = 10, Mann–Whitney test, p = 0.0233) thus suggesting that sialic acid blockade has the potential to inhibit the growth of prostate tumours. ( d ) Inhibition of sialylation in RM1 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( e ) Detection of α2-6 linked sialylated N -glycans in RM1 cells using SNA lectin flow cytometry. RM1 cells treated with 256 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p < 0.0001). ( f ) Luciferase tagged RM1 cells (control or pre-treated with 256 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via intra cardiac injection. Tumours were monitored over 15 days using in vivo bioluminescence imaging. ( g , h ) Pre-treatment of RM1 cells with P-SiaFNEtoc (to remove sialylated glycans) significantly reduced the number of skeletal tumours formed (Mann–Whitney test, p = 0.0454), the incidence of tumour in left tibias (Chi-square test, p = 0.0455), and significantly increased survival time in mice (Log-rank test, p = 0.012). ( i ) Micro-CT analysis demonstrated that P-SiaFNEtoc significantly alleviated bone destruction in the trabecular bone of tibias and increased trabecular bone volume (BV/TV, p = 0.0211) and trabecular number (Tb. N, p = 0.035) (n = 9, unpaired t test, ∗p < 0.05). Representative images are shown. Scale bar is 200 μm.
    Figure Legend Snippet: Sialic acid blockade can prevent/inhibit prostate cancer bone metastasis. ( a ) Inhibition of sialylation in TRAMPC2 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( b ) Detection of α2-6 linked sialylated N -glycans in TRAMPC2 cells using SNA lectin flow cytometry. TRAMPC2 cells treated with 64 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p = 0.0001). ( c ) Luciferase tagged TRAMPC2 cells (control or pre-treated with 64 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via sub-cutaneous injection and tumours were monitored using in vivo bioluminescence imaging. Pre-treatment of TRAMPC2 cells with P-SiaFNEtoc (which removed sialylated glycans) significantly reduced tumour burden over 6 weeks (n = 10, Mann–Whitney test, p = 0.0233) thus suggesting that sialic acid blockade has the potential to inhibit the growth of prostate tumours. ( d ) Inhibition of sialylation in RM1 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( e ) Detection of α2-6 linked sialylated N -glycans in RM1 cells using SNA lectin flow cytometry. RM1 cells treated with 256 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p < 0.0001). ( f ) Luciferase tagged RM1 cells (control or pre-treated with 256 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via intra cardiac injection. Tumours were monitored over 15 days using in vivo bioluminescence imaging. ( g , h ) Pre-treatment of RM1 cells with P-SiaFNEtoc (to remove sialylated glycans) significantly reduced the number of skeletal tumours formed (Mann–Whitney test, p = 0.0454), the incidence of tumour in left tibias (Chi-square test, p = 0.0455), and significantly increased survival time in mice (Log-rank test, p = 0.012). ( i ) Micro-CT analysis demonstrated that P-SiaFNEtoc significantly alleviated bone destruction in the trabecular bone of tibias and increased trabecular bone volume (BV/TV, p = 0.0211) and trabecular number (Tb. N, p = 0.035) (n = 9, unpaired t test, ∗p < 0.05). Representative images are shown. Scale bar is 200 μm.

    Techniques Used: Inhibition, Flow Cytometry, Binding Assay, Luciferase, Injection, In Vivo, Imaging, MANN-WHITNEY, Micro-CT

    sna lectin  (Vector Laboratories)


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    Vector Laboratories sna lectin
    Sna Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sna  (Vector Laboratories)


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    Vector Laboratories sna
    Characterization of adult bovine ileal organoid-derived monolayers. ( a ) A representative phase-contrast image of the monolayer at Day 6 of culture, exhibiting characteristic cobblestone morphology. Bar: 20 μm. ( b , c ) Representative scanning electron microscopy images of the monolayers at Day 6 of culture in low ( b ) and high ( c ) magnifications. The entire surface of the monolayer was uniformly covered by densely packed microvilli. Bars: 2 μm. ( d ) A representative transmission electron microscopy image of the monolayer at Day 5 of culture, demonstrating the formation of apical microvilli (MV) covered with glycocalyx (GLX), inter-cellular tight junctions (TJ) and desmosome (D). Bar: 500 nm. ( e ) The transepithelial electrical resistance (TEER) measured daily from Day 1 to 7 and apparent permeability ( P app ) of fluorescein isothiocyanate-dextran across the monolayer measured at Day 1, 3, and 5 of culture. Results are expressed as mean ± s.e.m. obtained from three independent experiments with at least two technical replicates per experiment using three biological replicates. ** p < 0.01, *** p < 0.001 compared with the previous day. ( f – i ) Immunofluorescence staining of the monolayers against F-actin (f, <t>red),</t> <t>E-cadherin</t> (g, green), Sambucus nigra agglutinin <t>(SNA)</t> (h, green), and EdU (i, cyan) at Day 6 of culture. Nuclei were stained with DAPI (blue). Top-down view (top) and cross-sectional images (z-stack, bottom) are shown. Bars: 20 μm. ( j ) RT-qPCR of the cells within the monolayers at Day 4 of culture. Relative expression levels of stem and lineage cell marker genes were evaluated using GAPDH , RPL0 and ACTB as internal controls. Results are expressed as mean ± s.e.m. obtained from two technical replicates from three biological replicates. LGR5 leucine rich repeat containing G protein-coupled receptor 5, CHGA chromogranin A, LYZC lysozyme C, MUC2 mucin 2, FABP2 fatty acid-binding protein 2, GAPDH : glyceraldehyde-3-phosphate dehydrogenase, RPL0 ribosomal protein L0 and ACTB β-actin.
    Sna, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Pathogen-epithelium interactions and inflammatory responses in Salmonella Dublin infections using ileal monolayer models derived from adult bovine organoids"

    Article Title: Pathogen-epithelium interactions and inflammatory responses in Salmonella Dublin infections using ileal monolayer models derived from adult bovine organoids

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-62407-2

    Characterization of adult bovine ileal organoid-derived monolayers. ( a ) A representative phase-contrast image of the monolayer at Day 6 of culture, exhibiting characteristic cobblestone morphology. Bar: 20 μm. ( b , c ) Representative scanning electron microscopy images of the monolayers at Day 6 of culture in low ( b ) and high ( c ) magnifications. The entire surface of the monolayer was uniformly covered by densely packed microvilli. Bars: 2 μm. ( d ) A representative transmission electron microscopy image of the monolayer at Day 5 of culture, demonstrating the formation of apical microvilli (MV) covered with glycocalyx (GLX), inter-cellular tight junctions (TJ) and desmosome (D). Bar: 500 nm. ( e ) The transepithelial electrical resistance (TEER) measured daily from Day 1 to 7 and apparent permeability ( P app ) of fluorescein isothiocyanate-dextran across the monolayer measured at Day 1, 3, and 5 of culture. Results are expressed as mean ± s.e.m. obtained from three independent experiments with at least two technical replicates per experiment using three biological replicates. ** p < 0.01, *** p < 0.001 compared with the previous day. ( f – i ) Immunofluorescence staining of the monolayers against F-actin (f, red), E-cadherin (g, green), Sambucus nigra agglutinin (SNA) (h, green), and EdU (i, cyan) at Day 6 of culture. Nuclei were stained with DAPI (blue). Top-down view (top) and cross-sectional images (z-stack, bottom) are shown. Bars: 20 μm. ( j ) RT-qPCR of the cells within the monolayers at Day 4 of culture. Relative expression levels of stem and lineage cell marker genes were evaluated using GAPDH , RPL0 and ACTB as internal controls. Results are expressed as mean ± s.e.m. obtained from two technical replicates from three biological replicates. LGR5 leucine rich repeat containing G protein-coupled receptor 5, CHGA chromogranin A, LYZC lysozyme C, MUC2 mucin 2, FABP2 fatty acid-binding protein 2, GAPDH : glyceraldehyde-3-phosphate dehydrogenase, RPL0 ribosomal protein L0 and ACTB β-actin.
    Figure Legend Snippet: Characterization of adult bovine ileal organoid-derived monolayers. ( a ) A representative phase-contrast image of the monolayer at Day 6 of culture, exhibiting characteristic cobblestone morphology. Bar: 20 μm. ( b , c ) Representative scanning electron microscopy images of the monolayers at Day 6 of culture in low ( b ) and high ( c ) magnifications. The entire surface of the monolayer was uniformly covered by densely packed microvilli. Bars: 2 μm. ( d ) A representative transmission electron microscopy image of the monolayer at Day 5 of culture, demonstrating the formation of apical microvilli (MV) covered with glycocalyx (GLX), inter-cellular tight junctions (TJ) and desmosome (D). Bar: 500 nm. ( e ) The transepithelial electrical resistance (TEER) measured daily from Day 1 to 7 and apparent permeability ( P app ) of fluorescein isothiocyanate-dextran across the monolayer measured at Day 1, 3, and 5 of culture. Results are expressed as mean ± s.e.m. obtained from three independent experiments with at least two technical replicates per experiment using three biological replicates. ** p < 0.01, *** p < 0.001 compared with the previous day. ( f – i ) Immunofluorescence staining of the monolayers against F-actin (f, red), E-cadherin (g, green), Sambucus nigra agglutinin (SNA) (h, green), and EdU (i, cyan) at Day 6 of culture. Nuclei were stained with DAPI (blue). Top-down view (top) and cross-sectional images (z-stack, bottom) are shown. Bars: 20 μm. ( j ) RT-qPCR of the cells within the monolayers at Day 4 of culture. Relative expression levels of stem and lineage cell marker genes were evaluated using GAPDH , RPL0 and ACTB as internal controls. Results are expressed as mean ± s.e.m. obtained from two technical replicates from three biological replicates. LGR5 leucine rich repeat containing G protein-coupled receptor 5, CHGA chromogranin A, LYZC lysozyme C, MUC2 mucin 2, FABP2 fatty acid-binding protein 2, GAPDH : glyceraldehyde-3-phosphate dehydrogenase, RPL0 ribosomal protein L0 and ACTB β-actin.

    Techniques Used: Derivative Assay, Electron Microscopy, Transmission Assay, Permeability, Immunofluorescence, Staining, Quantitative RT-PCR, Expressing, Marker, Binding Assay

    fitc conjugated sna lectin  (Vector Laboratories)


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    Vector Laboratories fitc conjugated sna lectin
    Sialic acid blockade can prevent/inhibit prostate cancer bone metastasis. ( a ) Inhibition of sialylation in TRAMPC2 cells using P-SiaFNEtoc detected using pan-specific Lectenz <t>lectin</t> flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( b ) Detection of α2-6 linked sialylated N -glycans in TRAMPC2 cells using <t>SNA</t> lectin flow cytometry. TRAMPC2 cells treated with 64 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p = 0.0001). ( c ) Luciferase tagged TRAMPC2 cells (control or pre-treated with 64 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via sub-cutaneous injection and tumours were monitored using in vivo bioluminescence imaging. Pre-treatment of TRAMPC2 cells with P-SiaFNEtoc (which removed sialylated glycans) significantly reduced tumour burden over 6 weeks (n = 10, Mann–Whitney test, p = 0.0233) thus suggesting that sialic acid blockade has the potential to inhibit the growth of prostate tumours. ( d ) Inhibition of sialylation in RM1 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( e ) Detection of α2-6 linked sialylated N -glycans in RM1 cells using SNA lectin flow cytometry. RM1 cells treated with 256 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p < 0.0001). ( f ) Luciferase tagged RM1 cells (control or pre-treated with 256 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via intra cardiac injection. Tumours were monitored over 15 days using in vivo bioluminescence imaging. ( g , h ) Pre-treatment of RM1 cells with P-SiaFNEtoc (to remove sialylated glycans) significantly reduced the number of skeletal tumours formed (Mann–Whitney test, p = 0.0454), the incidence of tumour in left tibias (Chi-square test, p = 0.0455), and significantly increased survival time in mice (Log-rank test, p = 0.012). ( i ) Micro-CT analysis demonstrated that P-SiaFNEtoc significantly alleviated bone destruction in the trabecular bone of tibias and increased trabecular bone volume (BV/TV, p = 0.0211) and trabecular number (Tb. N, p = 0.035) (n = 9, unpaired t test, ∗p < 0.05). Representative images are shown. Scale bar is 200 μm.
    Fitc Conjugated Sna Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated sna lectin/product/Vector Laboratories
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    Images

    1) Product Images from "Sialic acid blockade inhibits the metastatic spread of prostate cancer to bone"

    Article Title: Sialic acid blockade inhibits the metastatic spread of prostate cancer to bone

    Journal: eBioMedicine

    doi: 10.1016/j.ebiom.2024.105163

    Sialic acid blockade can prevent/inhibit prostate cancer bone metastasis. ( a ) Inhibition of sialylation in TRAMPC2 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( b ) Detection of α2-6 linked sialylated N -glycans in TRAMPC2 cells using SNA lectin flow cytometry. TRAMPC2 cells treated with 64 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p = 0.0001). ( c ) Luciferase tagged TRAMPC2 cells (control or pre-treated with 64 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via sub-cutaneous injection and tumours were monitored using in vivo bioluminescence imaging. Pre-treatment of TRAMPC2 cells with P-SiaFNEtoc (which removed sialylated glycans) significantly reduced tumour burden over 6 weeks (n = 10, Mann–Whitney test, p = 0.0233) thus suggesting that sialic acid blockade has the potential to inhibit the growth of prostate tumours. ( d ) Inhibition of sialylation in RM1 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( e ) Detection of α2-6 linked sialylated N -glycans in RM1 cells using SNA lectin flow cytometry. RM1 cells treated with 256 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p < 0.0001). ( f ) Luciferase tagged RM1 cells (control or pre-treated with 256 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via intra cardiac injection. Tumours were monitored over 15 days using in vivo bioluminescence imaging. ( g , h ) Pre-treatment of RM1 cells with P-SiaFNEtoc (to remove sialylated glycans) significantly reduced the number of skeletal tumours formed (Mann–Whitney test, p = 0.0454), the incidence of tumour in left tibias (Chi-square test, p = 0.0455), and significantly increased survival time in mice (Log-rank test, p = 0.012). ( i ) Micro-CT analysis demonstrated that P-SiaFNEtoc significantly alleviated bone destruction in the trabecular bone of tibias and increased trabecular bone volume (BV/TV, p = 0.0211) and trabecular number (Tb. N, p = 0.035) (n = 9, unpaired t test, ∗p < 0.05). Representative images are shown. Scale bar is 200 μm.
    Figure Legend Snippet: Sialic acid blockade can prevent/inhibit prostate cancer bone metastasis. ( a ) Inhibition of sialylation in TRAMPC2 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( b ) Detection of α2-6 linked sialylated N -glycans in TRAMPC2 cells using SNA lectin flow cytometry. TRAMPC2 cells treated with 64 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p = 0.0001). ( c ) Luciferase tagged TRAMPC2 cells (control or pre-treated with 64 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via sub-cutaneous injection and tumours were monitored using in vivo bioluminescence imaging. Pre-treatment of TRAMPC2 cells with P-SiaFNEtoc (which removed sialylated glycans) significantly reduced tumour burden over 6 weeks (n = 10, Mann–Whitney test, p = 0.0233) thus suggesting that sialic acid blockade has the potential to inhibit the growth of prostate tumours. ( d ) Inhibition of sialylation in RM1 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( e ) Detection of α2-6 linked sialylated N -glycans in RM1 cells using SNA lectin flow cytometry. RM1 cells treated with 256 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p < 0.0001). ( f ) Luciferase tagged RM1 cells (control or pre-treated with 256 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via intra cardiac injection. Tumours were monitored over 15 days using in vivo bioluminescence imaging. ( g , h ) Pre-treatment of RM1 cells with P-SiaFNEtoc (to remove sialylated glycans) significantly reduced the number of skeletal tumours formed (Mann–Whitney test, p = 0.0454), the incidence of tumour in left tibias (Chi-square test, p = 0.0455), and significantly increased survival time in mice (Log-rank test, p = 0.012). ( i ) Micro-CT analysis demonstrated that P-SiaFNEtoc significantly alleviated bone destruction in the trabecular bone of tibias and increased trabecular bone volume (BV/TV, p = 0.0211) and trabecular number (Tb. N, p = 0.035) (n = 9, unpaired t test, ∗p < 0.05). Representative images are shown. Scale bar is 200 μm.

    Techniques Used: Inhibition, Flow Cytometry, Binding Assay, Luciferase, Injection, In Vivo, Imaging, MANN-WHITNEY, Micro-CT

    fitc conjugated sna lectin  (Vector Laboratories)


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    Vector Laboratories fitc conjugated sna lectin
    Fitc Conjugated Sna Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sna fitc  (Vector Laboratories)


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    Vector Laboratories sna fitc
    OCSC1-F2 human OC cells were treated with ACM from three different patients (ACM 22, ACM24, ACM 26) for 7 days prior to staining with A) <t>FITC-tagged</t> <t>SNA;</t> B) FITC-tagged MAL-I; C) FITC-tagged MAL-II; D) FITC-tagged PNA. Histograms show results from ACM24. Graphs show mean ± SEM.
    Sna Fitc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Adipose microenvironment promotes hypersialylation of ovarian cancer cells"

    Article Title: Adipose microenvironment promotes hypersialylation of ovarian cancer cells

    Journal: bioRxiv

    doi: 10.1101/2024.05.13.593990

    OCSC1-F2 human OC cells were treated with ACM from three different patients (ACM 22, ACM24, ACM 26) for 7 days prior to staining with A) FITC-tagged SNA; B) FITC-tagged MAL-I; C) FITC-tagged MAL-II; D) FITC-tagged PNA. Histograms show results from ACM24. Graphs show mean ± SEM.
    Figure Legend Snippet: OCSC1-F2 human OC cells were treated with ACM from three different patients (ACM 22, ACM24, ACM 26) for 7 days prior to staining with A) FITC-tagged SNA; B) FITC-tagged MAL-I; C) FITC-tagged MAL-II; D) FITC-tagged PNA. Histograms show results from ACM24. Graphs show mean ± SEM.

    Techniques Used: Staining

    top panel , Cell surface sialic acid expression in mCherry+ TKO mouse OC cells in culture as detected by SNA, MAL-I, MAL-II and PNA; bottom panel , mCherry+ TKO mouse OC cells were injected i.p. in C57BL/6 mice and omental tumors were dissociated, stained with anti-CD45 and FITC-tagged lectins (n=5). Histograms show FITC staining from CD45-negative population. Note loss of SNA-low population and increase in Mal-I and Mal-II upon in vivo tumor formation. Histograms show data from one mouse. Similar results were observed in other mice. Double sided arrow shows SNA levels in tumors is comparable to SNA levels in TKO SNAhigh cells.
    Figure Legend Snippet: top panel , Cell surface sialic acid expression in mCherry+ TKO mouse OC cells in culture as detected by SNA, MAL-I, MAL-II and PNA; bottom panel , mCherry+ TKO mouse OC cells were injected i.p. in C57BL/6 mice and omental tumors were dissociated, stained with anti-CD45 and FITC-tagged lectins (n=5). Histograms show FITC staining from CD45-negative population. Note loss of SNA-low population and increase in Mal-I and Mal-II upon in vivo tumor formation. Histograms show data from one mouse. Similar results were observed in other mice. Double sided arrow shows SNA levels in tumors is comparable to SNA levels in TKO SNAhigh cells.

    Techniques Used: Expressing, Injection, Staining, In Vivo

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    Vector Laboratories sambucus nigra agglutinin sna
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    Vector Laboratories plant lectins sna
    The binding to mammary glands of three different cows was investigated for different IAV H5 proteins and a human H1 protein, as well as an IDV HEF and plant <t>lectins</t> MAL-II and <t>SNA.</t> AEC staining was used to visualize tissue binding.
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    Vector Laboratories sna
    (A) HEK293S GnTI-derived HKU1 NTD Fc and HKU1 NTD Fc + pA-LS displayed increased binding to BSM in comparison to their 293T-derived equivalents. (B) Presence of α2-3-SIA on BSM was confirmed by <t>utilizing</t> <t>MAL-II,</t> while <t>SNA</t> binding confirmed the presence of α2-6-SIA. Trimeric HKU1 NTD (GnTI- and 293-derived), pA-LS NP, and antibody control did not bind BSM.
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    Vector Laboratories fitc conjugated sna lectin
    Sialic acid blockade can prevent/inhibit prostate cancer bone metastasis. ( a ) Inhibition of sialylation in TRAMPC2 cells using P-SiaFNEtoc detected using pan-specific Lectenz <t>lectin</t> flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( b ) Detection of α2-6 linked sialylated N -glycans in TRAMPC2 cells using <t>SNA</t> lectin flow cytometry. TRAMPC2 cells treated with 64 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p = 0.0001). ( c ) Luciferase tagged TRAMPC2 cells (control or pre-treated with 64 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via sub-cutaneous injection and tumours were monitored using in vivo bioluminescence imaging. Pre-treatment of TRAMPC2 cells with P-SiaFNEtoc (which removed sialylated glycans) significantly reduced tumour burden over 6 weeks (n = 10, Mann–Whitney test, p = 0.0233) thus suggesting that sialic acid blockade has the potential to inhibit the growth of prostate tumours. ( d ) Inhibition of sialylation in RM1 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( e ) Detection of α2-6 linked sialylated N -glycans in RM1 cells using SNA lectin flow cytometry. RM1 cells treated with 256 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p < 0.0001). ( f ) Luciferase tagged RM1 cells (control or pre-treated with 256 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via intra cardiac injection. Tumours were monitored over 15 days using in vivo bioluminescence imaging. ( g , h ) Pre-treatment of RM1 cells with P-SiaFNEtoc (to remove sialylated glycans) significantly reduced the number of skeletal tumours formed (Mann–Whitney test, p = 0.0454), the incidence of tumour in left tibias (Chi-square test, p = 0.0455), and significantly increased survival time in mice (Log-rank test, p = 0.012). ( i ) Micro-CT analysis demonstrated that P-SiaFNEtoc significantly alleviated bone destruction in the trabecular bone of tibias and increased trabecular bone volume (BV/TV, p = 0.0211) and trabecular number (Tb. N, p = 0.035) (n = 9, unpaired t test, ∗p < 0.05). Representative images are shown. Scale bar is 200 μm.
    Fitc Conjugated Sna Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories sna lectin
    Sialic acid blockade can prevent/inhibit prostate cancer bone metastasis. ( a ) Inhibition of sialylation in TRAMPC2 cells using P-SiaFNEtoc detected using pan-specific Lectenz <t>lectin</t> flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( b ) Detection of α2-6 linked sialylated N -glycans in TRAMPC2 cells using <t>SNA</t> lectin flow cytometry. TRAMPC2 cells treated with 64 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p = 0.0001). ( c ) Luciferase tagged TRAMPC2 cells (control or pre-treated with 64 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via sub-cutaneous injection and tumours were monitored using in vivo bioluminescence imaging. Pre-treatment of TRAMPC2 cells with P-SiaFNEtoc (which removed sialylated glycans) significantly reduced tumour burden over 6 weeks (n = 10, Mann–Whitney test, p = 0.0233) thus suggesting that sialic acid blockade has the potential to inhibit the growth of prostate tumours. ( d ) Inhibition of sialylation in RM1 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( e ) Detection of α2-6 linked sialylated N -glycans in RM1 cells using SNA lectin flow cytometry. RM1 cells treated with 256 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p < 0.0001). ( f ) Luciferase tagged RM1 cells (control or pre-treated with 256 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via intra cardiac injection. Tumours were monitored over 15 days using in vivo bioluminescence imaging. ( g , h ) Pre-treatment of RM1 cells with P-SiaFNEtoc (to remove sialylated glycans) significantly reduced the number of skeletal tumours formed (Mann–Whitney test, p = 0.0454), the incidence of tumour in left tibias (Chi-square test, p = 0.0455), and significantly increased survival time in mice (Log-rank test, p = 0.012). ( i ) Micro-CT analysis demonstrated that P-SiaFNEtoc significantly alleviated bone destruction in the trabecular bone of tibias and increased trabecular bone volume (BV/TV, p = 0.0211) and trabecular number (Tb. N, p = 0.035) (n = 9, unpaired t test, ∗p < 0.05). Representative images are shown. Scale bar is 200 μm.
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    Vector Laboratories sna fitc
    OCSC1-F2 human OC cells were treated with ACM from three different patients (ACM 22, ACM24, ACM 26) for 7 days prior to staining with A) <t>FITC-tagged</t> <t>SNA;</t> B) FITC-tagged MAL-I; C) FITC-tagged MAL-II; D) FITC-tagged PNA. Histograms show results from ACM24. Graphs show mean ± SEM.
    Sna Fitc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The binding to mammary glands of three different cows was investigated for different IAV H5 proteins and a human H1 protein, as well as an IDV HEF and plant lectins MAL-II and SNA. AEC staining was used to visualize tissue binding.

    Journal: bioRxiv

    Article Title: The mammary glands of cows abundantly display receptors for circulating avian H5 viruses

    doi: 10.1101/2024.05.24.595667

    Figure Lengend Snippet: The binding to mammary glands of three different cows was investigated for different IAV H5 proteins and a human H1 protein, as well as an IDV HEF and plant lectins MAL-II and SNA. AEC staining was used to visualize tissue binding.

    Article Snippet: We also used commonly employed plant lectins SNA and MAL-II (biotinylated, Vector laboratories) to determine the distribution of α2,3 and α2,6 linked SIAs.

    Techniques: Binding Assay, Staining

    Hemagglutination assay with chicken erythrocytes with the lectins used in this study.

    Journal: bioRxiv

    Article Title: The mammary glands of cows abundantly display receptors for circulating avian H5 viruses

    doi: 10.1101/2024.05.24.595667

    Figure Lengend Snippet: Hemagglutination assay with chicken erythrocytes with the lectins used in this study.

    Article Snippet: We also used commonly employed plant lectins SNA and MAL-II (biotinylated, Vector laboratories) to determine the distribution of α2,3 and α2,6 linked SIAs.

    Techniques: Hemagglutination Assay

    (A) HEK293S GnTI-derived HKU1 NTD Fc and HKU1 NTD Fc + pA-LS displayed increased binding to BSM in comparison to their 293T-derived equivalents. (B) Presence of α2-3-SIA on BSM was confirmed by utilizing MAL-II, while SNA binding confirmed the presence of α2-6-SIA. Trimeric HKU1 NTD (GnTI- and 293-derived), pA-LS NP, and antibody control did not bind BSM.

    Journal: bioRxiv

    Article Title: The HCoV-HKU1 N-terminal domain binds a wide range of 9- O -acetylated sialic acids presented on different glycan cores

    doi: 10.1101/2024.05.24.595699

    Figure Lengend Snippet: (A) HEK293S GnTI-derived HKU1 NTD Fc and HKU1 NTD Fc + pA-LS displayed increased binding to BSM in comparison to their 293T-derived equivalents. (B) Presence of α2-3-SIA on BSM was confirmed by utilizing MAL-II, while SNA binding confirmed the presence of α2-6-SIA. Trimeric HKU1 NTD (GnTI- and 293-derived), pA-LS NP, and antibody control did not bind BSM.

    Article Snippet: As positive controls, SNA (B-1305, VectorLabs) and MAL-II (L-1260, VectorLabs) at 50 μg/mL were precomplexed with rabbit anti-biotin HRP (Bethyl) and donkey anti-rabbit Alexa-fluor 555 (Invitrogen) at a 4:2:1 molar ratio.

    Techniques: Derivative Assay, Binding Assay, Comparison

    Sialic acid blockade can prevent/inhibit prostate cancer bone metastasis. ( a ) Inhibition of sialylation in TRAMPC2 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( b ) Detection of α2-6 linked sialylated N -glycans in TRAMPC2 cells using SNA lectin flow cytometry. TRAMPC2 cells treated with 64 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p = 0.0001). ( c ) Luciferase tagged TRAMPC2 cells (control or pre-treated with 64 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via sub-cutaneous injection and tumours were monitored using in vivo bioluminescence imaging. Pre-treatment of TRAMPC2 cells with P-SiaFNEtoc (which removed sialylated glycans) significantly reduced tumour burden over 6 weeks (n = 10, Mann–Whitney test, p = 0.0233) thus suggesting that sialic acid blockade has the potential to inhibit the growth of prostate tumours. ( d ) Inhibition of sialylation in RM1 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( e ) Detection of α2-6 linked sialylated N -glycans in RM1 cells using SNA lectin flow cytometry. RM1 cells treated with 256 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p < 0.0001). ( f ) Luciferase tagged RM1 cells (control or pre-treated with 256 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via intra cardiac injection. Tumours were monitored over 15 days using in vivo bioluminescence imaging. ( g , h ) Pre-treatment of RM1 cells with P-SiaFNEtoc (to remove sialylated glycans) significantly reduced the number of skeletal tumours formed (Mann–Whitney test, p = 0.0454), the incidence of tumour in left tibias (Chi-square test, p = 0.0455), and significantly increased survival time in mice (Log-rank test, p = 0.012). ( i ) Micro-CT analysis demonstrated that P-SiaFNEtoc significantly alleviated bone destruction in the trabecular bone of tibias and increased trabecular bone volume (BV/TV, p = 0.0211) and trabecular number (Tb. N, p = 0.035) (n = 9, unpaired t test, ∗p < 0.05). Representative images are shown. Scale bar is 200 μm.

    Journal: eBioMedicine

    Article Title: Sialic acid blockade inhibits the metastatic spread of prostate cancer to bone

    doi: 10.1016/j.ebiom.2024.105163

    Figure Lengend Snippet: Sialic acid blockade can prevent/inhibit prostate cancer bone metastasis. ( a ) Inhibition of sialylation in TRAMPC2 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( b ) Detection of α2-6 linked sialylated N -glycans in TRAMPC2 cells using SNA lectin flow cytometry. TRAMPC2 cells treated with 64 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p = 0.0001). ( c ) Luciferase tagged TRAMPC2 cells (control or pre-treated with 64 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via sub-cutaneous injection and tumours were monitored using in vivo bioluminescence imaging. Pre-treatment of TRAMPC2 cells with P-SiaFNEtoc (which removed sialylated glycans) significantly reduced tumour burden over 6 weeks (n = 10, Mann–Whitney test, p = 0.0233) thus suggesting that sialic acid blockade has the potential to inhibit the growth of prostate tumours. ( d ) Inhibition of sialylation in RM1 cells using P-SiaFNEtoc detected using pan-specific Lectenz lectin flow cytometry. Cells were treated with a range of concentrations of P-SiaFNEtoc inhibitor from 2 μM to 512 μM for 72 h. The intensities were normalised to a DMSO control. ( e ) Detection of α2-6 linked sialylated N -glycans in RM1 cells using SNA lectin flow cytometry. RM1 cells treated with 256 μM P-SiaFNEtoc for 72 h had reduced levels of SNA binding indicating a reduction in α2-6 linked sialylation in these cells (unpaired t test, p < 0.0001). ( f ) Luciferase tagged RM1 cells (control or pre-treated with 256 μM P-SiaFNEtoc for 72 h) were injected into immunocompetent C57BL/6 mice via intra cardiac injection. Tumours were monitored over 15 days using in vivo bioluminescence imaging. ( g , h ) Pre-treatment of RM1 cells with P-SiaFNEtoc (to remove sialylated glycans) significantly reduced the number of skeletal tumours formed (Mann–Whitney test, p = 0.0454), the incidence of tumour in left tibias (Chi-square test, p = 0.0455), and significantly increased survival time in mice (Log-rank test, p = 0.012). ( i ) Micro-CT analysis demonstrated that P-SiaFNEtoc significantly alleviated bone destruction in the trabecular bone of tibias and increased trabecular bone volume (BV/TV, p = 0.0211) and trabecular number (Tb. N, p = 0.035) (n = 9, unpaired t test, ∗p < 0.05). Representative images are shown. Scale bar is 200 μm.

    Article Snippet: Slides were incubated for 3 h at room temperature with FITC-conjugated SNA lectin (Vector labs, FL-1301-2) at 1:500 or FITC-conjugated MAL I Lectin (Vector labs, FL-1311-2) at 1:500.

    Techniques: Inhibition, Flow Cytometry, Binding Assay, Luciferase, Injection, In Vivo, Imaging, MANN-WHITNEY, Micro-CT

    OCSC1-F2 human OC cells were treated with ACM from three different patients (ACM 22, ACM24, ACM 26) for 7 days prior to staining with A) FITC-tagged SNA; B) FITC-tagged MAL-I; C) FITC-tagged MAL-II; D) FITC-tagged PNA. Histograms show results from ACM24. Graphs show mean ± SEM.

    Journal: bioRxiv

    Article Title: Adipose microenvironment promotes hypersialylation of ovarian cancer cells

    doi: 10.1101/2024.05.13.593990

    Figure Lengend Snippet: OCSC1-F2 human OC cells were treated with ACM from three different patients (ACM 22, ACM24, ACM 26) for 7 days prior to staining with A) FITC-tagged SNA; B) FITC-tagged MAL-I; C) FITC-tagged MAL-II; D) FITC-tagged PNA. Histograms show results from ACM24. Graphs show mean ± SEM.

    Article Snippet: 1×10 6 cells were resuspended in 100 µL FACS buffer (1X PBS + 1% bovine serum albumin + 0.05% sodium azide) and stained for 30 mins on ice with the following lectins at 1:400 dilution: SNA-FITC (Vector Laboratories; RRID:AB_2336719), Mal-I-FITC (Bioworld 21761036), Mal-II-FITC (Bioworld 21511103) and PNA-FITC (RRID:AB_2315097).

    Techniques: Staining

    top panel , Cell surface sialic acid expression in mCherry+ TKO mouse OC cells in culture as detected by SNA, MAL-I, MAL-II and PNA; bottom panel , mCherry+ TKO mouse OC cells were injected i.p. in C57BL/6 mice and omental tumors were dissociated, stained with anti-CD45 and FITC-tagged lectins (n=5). Histograms show FITC staining from CD45-negative population. Note loss of SNA-low population and increase in Mal-I and Mal-II upon in vivo tumor formation. Histograms show data from one mouse. Similar results were observed in other mice. Double sided arrow shows SNA levels in tumors is comparable to SNA levels in TKO SNAhigh cells.

    Journal: bioRxiv

    Article Title: Adipose microenvironment promotes hypersialylation of ovarian cancer cells

    doi: 10.1101/2024.05.13.593990

    Figure Lengend Snippet: top panel , Cell surface sialic acid expression in mCherry+ TKO mouse OC cells in culture as detected by SNA, MAL-I, MAL-II and PNA; bottom panel , mCherry+ TKO mouse OC cells were injected i.p. in C57BL/6 mice and omental tumors were dissociated, stained with anti-CD45 and FITC-tagged lectins (n=5). Histograms show FITC staining from CD45-negative population. Note loss of SNA-low population and increase in Mal-I and Mal-II upon in vivo tumor formation. Histograms show data from one mouse. Similar results were observed in other mice. Double sided arrow shows SNA levels in tumors is comparable to SNA levels in TKO SNAhigh cells.

    Article Snippet: 1×10 6 cells were resuspended in 100 µL FACS buffer (1X PBS + 1% bovine serum albumin + 0.05% sodium azide) and stained for 30 mins on ice with the following lectins at 1:400 dilution: SNA-FITC (Vector Laboratories; RRID:AB_2336719), Mal-I-FITC (Bioworld 21761036), Mal-II-FITC (Bioworld 21511103) and PNA-FITC (RRID:AB_2315097).

    Techniques: Expressing, Injection, Staining, In Vivo