smarttm pcr cdna synthesis kit  (TaKaRa)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    SMARTer PCR cDNA Synthesis Kit
    Description:

    Catalog Number:
    634925
    Price:
    None
    Size:
    10 Rxns
    Category:
    cDNA synthesis kits cDNA synthesis
    Buy from Supplier


    Structured Review

    TaKaRa smarttm pcr cdna synthesis kit
    Lanes 1, 2, 3, 4: sampleI. Lanes 5, 6, 7, 8: sample II. Lanes 9, 10, 11, 12: sample III. Lane 13, marker. Lanes 1, 5, 9: <t>PCR</t> products using <t>cDNA</t> ligated Adaptor 1 as model, and G3PDH 3’primer and PCR Primer 1. Lanes 2, 6, 10: PCR products using cDNA ligated Adaptor 1 as model, and G3PDH 3’pmer and G3PDH 5’per; Lanes 3, 7, 11: PCR products using cDNA ligated Adaptor 2 as model, and G3PDH 3’primer and primer 1. Lanes 4, 8, 12: PCR products using cDNA ligatefd Adaptor 2 as model, and G3PDH 3’primer and G3PDH 5’primer.

    https://www.bioz.com/result/smarttm pcr cdna synthesis kit/product/TaKaRa
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    smarttm pcr cdna synthesis kit - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Construction and analysis of SSH cDNA library of human vascular endothelial cells related to gastrocarcinoma"

    Article Title: Construction and analysis of SSH cDNA library of human vascular endothelial cells related to gastrocarcinoma

    Journal: World Journal of Gastroenterology

    doi: 10.3748/wjg.v9.i11.2419

    Lanes 1, 2, 3, 4: sampleI. Lanes 5, 6, 7, 8: sample II. Lanes 9, 10, 11, 12: sample III. Lane 13, marker. Lanes 1, 5, 9: PCR products using cDNA ligated Adaptor 1 as model, and G3PDH 3’primer and PCR Primer 1. Lanes 2, 6, 10: PCR products using cDNA ligated Adaptor 1 as model, and G3PDH 3’pmer and G3PDH 5’per; Lanes 3, 7, 11: PCR products using cDNA ligated Adaptor 2 as model, and G3PDH 3’primer and primer 1. Lanes 4, 8, 12: PCR products using cDNA ligatefd Adaptor 2 as model, and G3PDH 3’primer and G3PDH 5’primer.
    Figure Legend Snippet: Lanes 1, 2, 3, 4: sampleI. Lanes 5, 6, 7, 8: sample II. Lanes 9, 10, 11, 12: sample III. Lane 13, marker. Lanes 1, 5, 9: PCR products using cDNA ligated Adaptor 1 as model, and G3PDH 3’primer and PCR Primer 1. Lanes 2, 6, 10: PCR products using cDNA ligated Adaptor 1 as model, and G3PDH 3’pmer and G3PDH 5’per; Lanes 3, 7, 11: PCR products using cDNA ligated Adaptor 2 as model, and G3PDH 3’primer and primer 1. Lanes 4, 8, 12: PCR products using cDNA ligatefd Adaptor 2 as model, and G3PDH 3’primer and G3PDH 5’primer.

    Techniques Used: Marker, Polymerase Chain Reaction

    Lanes 1, 2, 3 demonstrate 21, 24, 27 PCR cycles of cDNA products of stomach cancer tissue. Lanes 4, 5, 6 demonstrate 21, 24, 27 PCR cycles of cDNA products of normal stomach tissue. Lanes 7, 8, 9 demonstrate 15, 18, 21 PCR cycles of cDNA products of placental tissue. Lane 10, marker.
    Figure Legend Snippet: Lanes 1, 2, 3 demonstrate 21, 24, 27 PCR cycles of cDNA products of stomach cancer tissue. Lanes 4, 5, 6 demonstrate 21, 24, 27 PCR cycles of cDNA products of normal stomach tissue. Lanes 7, 8, 9 demonstrate 15, 18, 21 PCR cycles of cDNA products of placental tissue. Lane 10, marker.

    Techniques Used: Polymerase Chain Reaction, Marker

    2) Product Images from "Screening of Differentially Expressed Microsporidia Genes from Nosema ceranae Infected Honey Bees by Suppression Subtractive Hybridization"

    Article Title: Screening of Differentially Expressed Microsporidia Genes from Nosema ceranae Infected Honey Bees by Suppression Subtractive Hybridization

    Journal: Insects

    doi: 10.3390/insects11030199

    Distribution of expression sequence tags (ESTs) belong to eighteen N. ceranae specific genes and dot-blot hybridization test the cDNA library of N. ceranae -infected cDNA library. ( A ) The probes that came from subtractive PCR products of the N. ceranae -infected cDNA library was used to test the cDNA library of N. ceranae -infected cDNA library; ( B ) the probes that came from unsubtractive PCR products of the control honey bee cDNA library was used to test the cDNA library of the N. ceranae -infected cDNA library.
    Figure Legend Snippet: Distribution of expression sequence tags (ESTs) belong to eighteen N. ceranae specific genes and dot-blot hybridization test the cDNA library of N. ceranae -infected cDNA library. ( A ) The probes that came from subtractive PCR products of the N. ceranae -infected cDNA library was used to test the cDNA library of N. ceranae -infected cDNA library; ( B ) the probes that came from unsubtractive PCR products of the control honey bee cDNA library was used to test the cDNA library of the N. ceranae -infected cDNA library.

    Techniques Used: Expressing, Sequencing, Dot Blot, Hybridization, cDNA Library Assay, Infection, Polymerase Chain Reaction

    Screening and identification of differential expressed sequence tags (ESTs) from the Nosema ceranae infected honey bee by suppression subtractive hybridization (SSH) and Dot-blotting. ESTs insert size of ( A ) forward and ( D ) reverse SSH complementary DNA (cDNA) libraries were confirmed by colony PCR. ( B ) Forward subtractive library (fsl, differential ESTs in infected group) hybridized with forward-subtracted probe and ( C ) with reverse-subtracted probe. ( E ) Reverse subtractive library (rsl, differential ESTs in control group) hybridized with reverse-subtracted probe and ( F ) with forward-subtracted probe. M = 100 bp DNA ladder. The arrows indicated the differential ESTs for sequencing.
    Figure Legend Snippet: Screening and identification of differential expressed sequence tags (ESTs) from the Nosema ceranae infected honey bee by suppression subtractive hybridization (SSH) and Dot-blotting. ESTs insert size of ( A ) forward and ( D ) reverse SSH complementary DNA (cDNA) libraries were confirmed by colony PCR. ( B ) Forward subtractive library (fsl, differential ESTs in infected group) hybridized with forward-subtracted probe and ( C ) with reverse-subtracted probe. ( E ) Reverse subtractive library (rsl, differential ESTs in control group) hybridized with reverse-subtracted probe and ( F ) with forward-subtracted probe. M = 100 bp DNA ladder. The arrows indicated the differential ESTs for sequencing.

    Techniques Used: Sequencing, Infection, Hybridization, Polymerase Chain Reaction

    Related Articles

    Selection:

    Article Title: Assessment of an Organ-Specific de Novo Transcriptome of the Nematode Trap-Crop, Solanum sisymbriifolium
    Article Snippet: .. The Iso-Seq libraries for four organs, root, stem, leaf and bud, were prepared for Isoform Sequencing (Iso-Seq) using the Clontech SMARTer PCR cDNA Synthesis Kit and the BluePippin Size Selection System protocol as described by Pacific Biosciences ( https://goo.gl/ij71Hh ) with the following modifications. .. For cDNA conversion, 3 µg of total RNA was put into each Clonetech SMARTer reaction.

    Article Title: Long-read assays shed new light on the transcriptome complexity of a viral pathogen
    Article Snippet: .. cDNA synthesisCopy DNAs were generated from polyA(+) RNA fractions using SMARTer PCR cDNA Synthesis Kits (Clontech) according to ‘PacBio Isoform Sequencing (Iso-Seq) using Clontech SMARTer PCR cDNA Synthesis Kit and No Size Selection’ protocols. .. Samples from different infection time points (1-, 4-, 8-, and 12-h p.i.) were mixed for RSII library preparation, whereas 1-, 2-, 3-, 4-, 6-, and 8-h p.i. samples were mixed for Sequel sequencing.

    Agarose Gel Electrophoresis:

    Article Title: Construction of a hepatic stellate cells subtracted cDNA library of differentially expressed genes in normal mice and mice with Schistosomiasis japonica *
    Article Snippet: .. High yields of full-length cDNAs were generated from 1 μg total RNA of normal and fibrosis HSCs using SMART PCR cDNA synthesis kit (Clontech, USA). cDNAs (0.2 μg) were electrophosed on a 1.0% agarose gel, transferred onto Hybond N+ Membrane (Roch, Germany) and hybridized with DIG-labelled clone probes. .. These probes were generated by PCR amplification of the relevant inserts, adaptor-cutting and column-purification.

    Sequencing:

    Article Title: Assessment of an Organ-Specific de Novo Transcriptome of the Nematode Trap-Crop, Solanum sisymbriifolium
    Article Snippet: .. The Iso-Seq libraries for four organs, root, stem, leaf and bud, were prepared for Isoform Sequencing (Iso-Seq) using the Clontech SMARTer PCR cDNA Synthesis Kit and the BluePippin Size Selection System protocol as described by Pacific Biosciences ( https://goo.gl/ij71Hh ) with the following modifications. .. For cDNA conversion, 3 µg of total RNA was put into each Clonetech SMARTer reaction.

    Article Title: Long-read assays shed new light on the transcriptome complexity of a viral pathogen
    Article Snippet: .. cDNA synthesisCopy DNAs were generated from polyA(+) RNA fractions using SMARTer PCR cDNA Synthesis Kits (Clontech) according to ‘PacBio Isoform Sequencing (Iso-Seq) using Clontech SMARTer PCR cDNA Synthesis Kit and No Size Selection’ protocols. .. Samples from different infection time points (1-, 4-, 8-, and 12-h p.i.) were mixed for RSII library preparation, whereas 1-, 2-, 3-, 4-, 6-, and 8-h p.i. samples were mixed for Sequel sequencing.

    cDNA Library Assay:

    Article Title: PR-10, defensin and cold dehydrin genes are among those over expressed in Oxytropis (Fabaceae) species adapted to the arctic
    Article Snippet: .. Suppressive subtraction cDNA library construction Extracted RNA from two plantlets of a species were pooled prior to complimentary DNA (cDNA) synthesis, and 1 mg of this pool was used as template for cDNA synthesis using the SuperSmart PCR cDNA Synthesis kit (Clontech, Mountain View, CA). .. Genes specifically expressed in either the arctic or in the temperate species were isolated by applying the suppression subtractive hybridization strategy (Diatchenko et al. ) using the PCR-select cDNA subtraction kit (Clontech, Mountain View, CA).

    Generated:

    Article Title: Construction of a hepatic stellate cells subtracted cDNA library of differentially expressed genes in normal mice and mice with Schistosomiasis japonica *
    Article Snippet: .. High yields of full-length cDNAs were generated from 1 μg total RNA of normal and fibrosis HSCs using SMART PCR cDNA synthesis kit (Clontech, USA). cDNAs (0.2 μg) were electrophosed on a 1.0% agarose gel, transferred onto Hybond N+ Membrane (Roch, Germany) and hybridized with DIG-labelled clone probes. .. These probes were generated by PCR amplification of the relevant inserts, adaptor-cutting and column-purification.

    Article Title: Identification of differentially expressed genes in normal mucosa, adenoma and adenocarcinoma of colon by SSH
    Article Snippet: .. High yields of full-length cDNAs were generated from 1 μg total RNA of normal mucosa, adenoma and adenocarcinoma using SMART PCR cDNA Synthesis kit (Clontech, USA). .. 0.2 μg cDNAs were electrop hosed on a 1.0% agarose gel, transferred onto Hybond N+ Membrane (Boeheringer Mannheim, Germany) and hybridized with DIG-labeled clone probes.

    Article Title: A novel method to isolate the common fraction of two DNA samples: hybrid specific amplification (HSA)
    Article Snippet: .. Double-stranded cDNA was generated with the SMART PCR cDNA Synthesis Kit (Clontech). .. Aliquots of 2 µg of Qiagen-prepared BAC DNA and 1 µg of SMART cDNA PCR product were digested by Rsa I (15 U) for 90 min at 37°C.

    Article Title: Long-read assays shed new light on the transcriptome complexity of a viral pathogen
    Article Snippet: .. cDNA synthesisCopy DNAs were generated from polyA(+) RNA fractions using SMARTer PCR cDNA Synthesis Kits (Clontech) according to ‘PacBio Isoform Sequencing (Iso-Seq) using Clontech SMARTer PCR cDNA Synthesis Kit and No Size Selection’ protocols. .. Samples from different infection time points (1-, 4-, 8-, and 12-h p.i.) were mixed for RSII library preparation, whereas 1-, 2-, 3-, 4-, 6-, and 8-h p.i. samples were mixed for Sequel sequencing.

    Polymerase Chain Reaction:

    Article Title: Assessment of an Organ-Specific de Novo Transcriptome of the Nematode Trap-Crop, Solanum sisymbriifolium
    Article Snippet: .. The Iso-Seq libraries for four organs, root, stem, leaf and bud, were prepared for Isoform Sequencing (Iso-Seq) using the Clontech SMARTer PCR cDNA Synthesis Kit and the BluePippin Size Selection System protocol as described by Pacific Biosciences ( https://goo.gl/ij71Hh ) with the following modifications. .. For cDNA conversion, 3 µg of total RNA was put into each Clonetech SMARTer reaction.

    Article Title: Construction of a hepatic stellate cells subtracted cDNA library of differentially expressed genes in normal mice and mice with Schistosomiasis japonica *
    Article Snippet: .. High yields of full-length cDNAs were generated from 1 μg total RNA of normal and fibrosis HSCs using SMART PCR cDNA synthesis kit (Clontech, USA). cDNAs (0.2 μg) were electrophosed on a 1.0% agarose gel, transferred onto Hybond N+ Membrane (Roch, Germany) and hybridized with DIG-labelled clone probes. .. These probes were generated by PCR amplification of the relevant inserts, adaptor-cutting and column-purification.

    Article Title: Decreased endothelin receptor B expression in large primary uveal melanomas is associated with early clinical metastasis and short survival
    Article Snippet: .. Suppression subtractive hybridisation (SSH) Double stranded cDNA was prepared from 2 μg of total RNA using the SMART™ PCR cDNA synthesis kit (Clontech, Cowley, Oxford, UK), following the manufacturer's protocol. .. SSH was performed using the PCR-Select™ cDNA subtraction kit (Clontech), again, following the manufacturer's recommended protocol.

    Article Title: Identification of differentially expressed genes in normal mucosa, adenoma and adenocarcinoma of colon by SSH
    Article Snippet: .. High yields of full-length cDNAs were generated from 1 μg total RNA of normal mucosa, adenoma and adenocarcinoma using SMART PCR cDNA Synthesis kit (Clontech, USA). .. 0.2 μg cDNAs were electrop hosed on a 1.0% agarose gel, transferred onto Hybond N+ Membrane (Boeheringer Mannheim, Germany) and hybridized with DIG-labeled clone probes.

    Article Title: A novel method to isolate the common fraction of two DNA samples: hybrid specific amplification (HSA)
    Article Snippet: .. Double-stranded cDNA was generated with the SMART PCR cDNA Synthesis Kit (Clontech). .. Aliquots of 2 µg of Qiagen-prepared BAC DNA and 1 µg of SMART cDNA PCR product were digested by Rsa I (15 U) for 90 min at 37°C.

    Article Title: Cloning and expression of an inhibitor of microbial metalloproteinases from insects contributing to innate immunity
    Article Snippet: .. Subtractive hybridization and suppressive PCR were carried out with the SMART PCR cDNA Synthesis Kit (Clontech) and the PCR Select cDNA Subtraction Kit (Clontech), followed by a screening with non-radioactive digoxygenin dot blots (as previously described in [ ]). .. Among the clones obtained, we identified a fragment of the IMPI cDNA.

    Article Title: PR-10, defensin and cold dehydrin genes are among those over expressed in Oxytropis (Fabaceae) species adapted to the arctic
    Article Snippet: .. Suppressive subtraction cDNA library construction Extracted RNA from two plantlets of a species were pooled prior to complimentary DNA (cDNA) synthesis, and 1 mg of this pool was used as template for cDNA synthesis using the SuperSmart PCR cDNA Synthesis kit (Clontech, Mountain View, CA). .. Genes specifically expressed in either the arctic or in the temperate species were isolated by applying the suppression subtractive hybridization strategy (Diatchenko et al. ) using the PCR-select cDNA subtraction kit (Clontech, Mountain View, CA).

    Article Title: Long-read assays shed new light on the transcriptome complexity of a viral pathogen
    Article Snippet: .. cDNA synthesisCopy DNAs were generated from polyA(+) RNA fractions using SMARTer PCR cDNA Synthesis Kits (Clontech) according to ‘PacBio Isoform Sequencing (Iso-Seq) using Clontech SMARTer PCR cDNA Synthesis Kit and No Size Selection’ protocols. .. Samples from different infection time points (1-, 4-, 8-, and 12-h p.i.) were mixed for RSII library preparation, whereas 1-, 2-, 3-, 4-, 6-, and 8-h p.i. samples were mixed for Sequel sequencing.

    Hybridization:

    Article Title: Decreased endothelin receptor B expression in large primary uveal melanomas is associated with early clinical metastasis and short survival
    Article Snippet: .. Suppression subtractive hybridisation (SSH) Double stranded cDNA was prepared from 2 μg of total RNA using the SMART™ PCR cDNA synthesis kit (Clontech, Cowley, Oxford, UK), following the manufacturer's protocol. .. SSH was performed using the PCR-Select™ cDNA subtraction kit (Clontech), again, following the manufacturer's recommended protocol.

    Article Title: Cloning and expression of an inhibitor of microbial metalloproteinases from insects contributing to innate immunity
    Article Snippet: .. Subtractive hybridization and suppressive PCR were carried out with the SMART PCR cDNA Synthesis Kit (Clontech) and the PCR Select cDNA Subtraction Kit (Clontech), followed by a screening with non-radioactive digoxygenin dot blots (as previously described in [ ]). .. Among the clones obtained, we identified a fragment of the IMPI cDNA.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    TaKaRa smarttm race cdna amplification kit
    5′ <t>RACE</t> analysis of the ACSL1 <t>cDNA</t> synthesized from skeletal muscle. ( a ) ACSL1 5′ RACE products from nested PCR were analyzed by agarose gel electrophoresis. Arrowheads indicate the resulting DNA bands. ( b ) Sequence of the ACSL1 promoter and the downstream region. The positions of identified TSS were marked with arrows. The primers used for 5′ RACE analysis are underlined. The translational start site (ATG) is shown by a double underline. The 5′ untranslated region and exons are shown in capitals, and introns are shown in small letters with the total intron sizes shown.
    Smarttm Race Cdna Amplification Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smarttm race cdna amplification kit/product/TaKaRa
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    smarttm race cdna amplification kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    TaKaRa smarttm pcr cdna synthesis kit
    Lanes 1, 2, 3, 4: sampleI. Lanes 5, 6, 7, 8: sample II. Lanes 9, 10, 11, 12: sample III. Lane 13, marker. Lanes 1, 5, 9: <t>PCR</t> products using <t>cDNA</t> ligated Adaptor 1 as model, and G3PDH 3’primer and PCR Primer 1. Lanes 2, 6, 10: PCR products using cDNA ligated Adaptor 1 as model, and G3PDH 3’pmer and G3PDH 5’per; Lanes 3, 7, 11: PCR products using cDNA ligated Adaptor 2 as model, and G3PDH 3’primer and primer 1. Lanes 4, 8, 12: PCR products using cDNA ligatefd Adaptor 2 as model, and G3PDH 3’primer and G3PDH 5’primer.
    Smarttm Pcr Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smarttm pcr cdna synthesis kit/product/TaKaRa
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    smarttm pcr cdna synthesis kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    5′ RACE analysis of the ACSL1 cDNA synthesized from skeletal muscle. ( a ) ACSL1 5′ RACE products from nested PCR were analyzed by agarose gel electrophoresis. Arrowheads indicate the resulting DNA bands. ( b ) Sequence of the ACSL1 promoter and the downstream region. The positions of identified TSS were marked with arrows. The primers used for 5′ RACE analysis are underlined. The translational start site (ATG) is shown by a double underline. The 5′ untranslated region and exons are shown in capitals, and introns are shown in small letters with the total intron sizes shown.

    Journal: Scientific Reports

    Article Title: Characterization of the promoter region of the bovine long-chain acyl-CoA synthetase 1 gene: Roles of E2F1, Sp1, KLF15, and E2F4

    doi: 10.1038/srep19661

    Figure Lengend Snippet: 5′ RACE analysis of the ACSL1 cDNA synthesized from skeletal muscle. ( a ) ACSL1 5′ RACE products from nested PCR were analyzed by agarose gel electrophoresis. Arrowheads indicate the resulting DNA bands. ( b ) Sequence of the ACSL1 promoter and the downstream region. The positions of identified TSS were marked with arrows. The primers used for 5′ RACE analysis are underlined. The translational start site (ATG) is shown by a double underline. The 5′ untranslated region and exons are shown in capitals, and introns are shown in small letters with the total intron sizes shown.

    Article Snippet: Rapid amplification of cDNA ends (5′ RACE) The transcription initiation site (TSS) of the bovine ACSL1 gene was determined using a BD SMARTTM RACE cDNA amplification kit (Clontech Inc, CA, USA) according to the manufacturer’s instructions.

    Techniques: Synthesized, Nested PCR, Agarose Gel Electrophoresis, Sequencing

    Lanes 1, 2, 3, 4: sampleI. Lanes 5, 6, 7, 8: sample II. Lanes 9, 10, 11, 12: sample III. Lane 13, marker. Lanes 1, 5, 9: PCR products using cDNA ligated Adaptor 1 as model, and G3PDH 3’primer and PCR Primer 1. Lanes 2, 6, 10: PCR products using cDNA ligated Adaptor 1 as model, and G3PDH 3’pmer and G3PDH 5’per; Lanes 3, 7, 11: PCR products using cDNA ligated Adaptor 2 as model, and G3PDH 3’primer and primer 1. Lanes 4, 8, 12: PCR products using cDNA ligatefd Adaptor 2 as model, and G3PDH 3’primer and G3PDH 5’primer.

    Journal: World Journal of Gastroenterology

    Article Title: Construction and analysis of SSH cDNA library of human vascular endothelial cells related to gastrocarcinoma

    doi: 10.3748/wjg.v9.i11.2419

    Figure Lengend Snippet: Lanes 1, 2, 3, 4: sampleI. Lanes 5, 6, 7, 8: sample II. Lanes 9, 10, 11, 12: sample III. Lane 13, marker. Lanes 1, 5, 9: PCR products using cDNA ligated Adaptor 1 as model, and G3PDH 3’primer and PCR Primer 1. Lanes 2, 6, 10: PCR products using cDNA ligated Adaptor 1 as model, and G3PDH 3’pmer and G3PDH 5’per; Lanes 3, 7, 11: PCR products using cDNA ligated Adaptor 2 as model, and G3PDH 3’primer and primer 1. Lanes 4, 8, 12: PCR products using cDNA ligatefd Adaptor 2 as model, and G3PDH 3’primer and G3PDH 5’primer.

    Article Snippet: About 100 ng total RNA was used to synthesize the first strand of cDNA with SMARTTM PCR cDNA Synthesis Kit (Clontech Company), then amplified by LD-PCR with 15, 18, 21, 24, 27 cycles separately and analyzed through 1.2% agarose gel electrophoresis in order to get the perfect cycle number with which we harvested a suitable amount rather than a superfluous one to build the library.

    Techniques: Marker, Polymerase Chain Reaction

    Lanes 1, 2, 3 demonstrate 21, 24, 27 PCR cycles of cDNA products of stomach cancer tissue. Lanes 4, 5, 6 demonstrate 21, 24, 27 PCR cycles of cDNA products of normal stomach tissue. Lanes 7, 8, 9 demonstrate 15, 18, 21 PCR cycles of cDNA products of placental tissue. Lane 10, marker.

    Journal: World Journal of Gastroenterology

    Article Title: Construction and analysis of SSH cDNA library of human vascular endothelial cells related to gastrocarcinoma

    doi: 10.3748/wjg.v9.i11.2419

    Figure Lengend Snippet: Lanes 1, 2, 3 demonstrate 21, 24, 27 PCR cycles of cDNA products of stomach cancer tissue. Lanes 4, 5, 6 demonstrate 21, 24, 27 PCR cycles of cDNA products of normal stomach tissue. Lanes 7, 8, 9 demonstrate 15, 18, 21 PCR cycles of cDNA products of placental tissue. Lane 10, marker.

    Article Snippet: About 100 ng total RNA was used to synthesize the first strand of cDNA with SMARTTM PCR cDNA Synthesis Kit (Clontech Company), then amplified by LD-PCR with 15, 18, 21, 24, 27 cycles separately and analyzed through 1.2% agarose gel electrophoresis in order to get the perfect cycle number with which we harvested a suitable amount rather than a superfluous one to build the library.

    Techniques: Polymerase Chain Reaction, Marker

    Distribution of expression sequence tags (ESTs) belong to eighteen N. ceranae specific genes and dot-blot hybridization test the cDNA library of N. ceranae -infected cDNA library. ( A ) The probes that came from subtractive PCR products of the N. ceranae -infected cDNA library was used to test the cDNA library of N. ceranae -infected cDNA library; ( B ) the probes that came from unsubtractive PCR products of the control honey bee cDNA library was used to test the cDNA library of the N. ceranae -infected cDNA library.

    Journal: Insects

    Article Title: Screening of Differentially Expressed Microsporidia Genes from Nosema ceranae Infected Honey Bees by Suppression Subtractive Hybridization

    doi: 10.3390/insects11030199

    Figure Lengend Snippet: Distribution of expression sequence tags (ESTs) belong to eighteen N. ceranae specific genes and dot-blot hybridization test the cDNA library of N. ceranae -infected cDNA library. ( A ) The probes that came from subtractive PCR products of the N. ceranae -infected cDNA library was used to test the cDNA library of N. ceranae -infected cDNA library; ( B ) the probes that came from unsubtractive PCR products of the control honey bee cDNA library was used to test the cDNA library of the N. ceranae -infected cDNA library.

    Article Snippet: Single-stranded and double-stranded cDNA were synthesized from the infected and control RNA pools with the SMARTTM PCR cDNA Synthesis Kit (Takara, Kyoto, Japan), according to the manufacturer’s protocol.

    Techniques: Expressing, Sequencing, Dot Blot, Hybridization, cDNA Library Assay, Infection, Polymerase Chain Reaction

    Screening and identification of differential expressed sequence tags (ESTs) from the Nosema ceranae infected honey bee by suppression subtractive hybridization (SSH) and Dot-blotting. ESTs insert size of ( A ) forward and ( D ) reverse SSH complementary DNA (cDNA) libraries were confirmed by colony PCR. ( B ) Forward subtractive library (fsl, differential ESTs in infected group) hybridized with forward-subtracted probe and ( C ) with reverse-subtracted probe. ( E ) Reverse subtractive library (rsl, differential ESTs in control group) hybridized with reverse-subtracted probe and ( F ) with forward-subtracted probe. M = 100 bp DNA ladder. The arrows indicated the differential ESTs for sequencing.

    Journal: Insects

    Article Title: Screening of Differentially Expressed Microsporidia Genes from Nosema ceranae Infected Honey Bees by Suppression Subtractive Hybridization

    doi: 10.3390/insects11030199

    Figure Lengend Snippet: Screening and identification of differential expressed sequence tags (ESTs) from the Nosema ceranae infected honey bee by suppression subtractive hybridization (SSH) and Dot-blotting. ESTs insert size of ( A ) forward and ( D ) reverse SSH complementary DNA (cDNA) libraries were confirmed by colony PCR. ( B ) Forward subtractive library (fsl, differential ESTs in infected group) hybridized with forward-subtracted probe and ( C ) with reverse-subtracted probe. ( E ) Reverse subtractive library (rsl, differential ESTs in control group) hybridized with reverse-subtracted probe and ( F ) with forward-subtracted probe. M = 100 bp DNA ladder. The arrows indicated the differential ESTs for sequencing.

    Article Snippet: Single-stranded and double-stranded cDNA were synthesized from the infected and control RNA pools with the SMARTTM PCR cDNA Synthesis Kit (Takara, Kyoto, Japan), according to the manufacturer’s protocol.

    Techniques: Sequencing, Infection, Hybridization, Polymerase Chain Reaction

    Expression levels of the porin gene between uninfected and infected nymphal and female ticks. (A) Porin gene expression level in B. microti -infected or -uninfected nymphal H. longicornis . (B) Gel electrophoresis analysis of conventional PCR products of Babesia β- tubulin (1,341 bp) in B. microti -infected and -uninfected female ticks at engorgement. Lane 1, infected tick sample; lane 2, positive control; lane 3, uninfected tick sample. (C) SSH analysis of porin expression levels in infected ticks. No or weak bands were visualized on a gel for PCR products of porin amplified from cDNA of infected ticks subtracted with that of uninfected ticks. Bright bands were visualized on a gel for PCR products of porin amplified from cDNA of uninfected ticks subtracted with that of infected ticks. Lanes 1, 5, 9, and 13: PCR products amplified by 18 cycles from porin gene; lanes 2, 6, 10, and 14: PCR products amplified by 23 cycles; lanes 3, 7, 11, and 15: PCR products amplified by 28 cycles; lanes 4, 8, 12, and 16: PCR products amplified by 33 cycles. (D) Real-time PCR analysis of porin mRNA expression in whole body of B. microti -infected and -uninfected engorged female ticks. The bar indicates the median with 95% CI of three biological repeats. The asterisks above the bar indicate a significant difference in porin / GAPDH between uninfected and B. microti -infected ticks (** p

    Journal: Frontiers in Physiology

    Article Title: Porin Expression Profiles in Haemaphysalis longicornis Infected With Babesia microti

    doi: 10.3389/fphys.2020.00502

    Figure Lengend Snippet: Expression levels of the porin gene between uninfected and infected nymphal and female ticks. (A) Porin gene expression level in B. microti -infected or -uninfected nymphal H. longicornis . (B) Gel electrophoresis analysis of conventional PCR products of Babesia β- tubulin (1,341 bp) in B. microti -infected and -uninfected female ticks at engorgement. Lane 1, infected tick sample; lane 2, positive control; lane 3, uninfected tick sample. (C) SSH analysis of porin expression levels in infected ticks. No or weak bands were visualized on a gel for PCR products of porin amplified from cDNA of infected ticks subtracted with that of uninfected ticks. Bright bands were visualized on a gel for PCR products of porin amplified from cDNA of uninfected ticks subtracted with that of infected ticks. Lanes 1, 5, 9, and 13: PCR products amplified by 18 cycles from porin gene; lanes 2, 6, 10, and 14: PCR products amplified by 23 cycles; lanes 3, 7, 11, and 15: PCR products amplified by 28 cycles; lanes 4, 8, 12, and 16: PCR products amplified by 33 cycles. (D) Real-time PCR analysis of porin mRNA expression in whole body of B. microti -infected and -uninfected engorged female ticks. The bar indicates the median with 95% CI of three biological repeats. The asterisks above the bar indicate a significant difference in porin / GAPDH between uninfected and B. microti -infected ticks (** p

    Article Snippet: Forward and reverse suppression subtraction cDNA libraries were constructed using the Super SMARTTM PCR cDNA synthesis kit according to the manufacturer’s instructions (Clontech, Mountain View, CA, United States).

    Techniques: Expressing, Infection, Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Positive Control, Amplification, Real-time Polymerase Chain Reaction