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Journal: International Journal of Molecular Sciences
Article Title: Runx3 Induces a Cell Shape Change and Suppresses Migration and Metastasis of Melanoma Cells by Altering a Transcriptional Profile
doi: 10.3390/ijms22042219
Figure Lengend Snippet: Microarray analysis uncovered an altered and specific transcriptional profile underlying the cell shape change and the suppression of the metastatic potential by Runx3. ( A ) Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations assigned different repertoires of differentially expressed genes (DEGs) that were regulated by Runx3 in B16-F10 cells and, in the case of B16-F0 cells vs. B16-F10 cells, to the terms relevant to the actin cytoskeleton and adhesion. Each GO/KEGG annotation term is inscribed as a caption. The number of DEGs within a repertoire is described at x axis. The columns for the downregulated and the upregulated DEGs are shown in different colors as explained at the top of the figure., Down/Up by Runx3 indicates the appearance of DEGs in the case of B16-F10/Runx3 cells vs. mock control B16-F10 cells. Down/Up in B16-F0 indicates the appearance of DEGs in the case of B16-F0 cells vs. B16-F10 cells. Down/Up in common indicates the appearance of same DEGs in both cases. ( B ) Runx3 upregulated the expression of extracellular matrix (ECM) genes. The summary table indicates the gene names and their corresponding fold changes. ( C ) Runx3 regulated the expression of a list of DEGs that were inversely associated with an increase in the metastatic potential of B16-F10 cells compared to B16-F0 cells. The heatmap shows the gene names and their fold changes in the cases of B16-F10/Runx3 cells vs. mock control B16-F10 cells and B16-F0 cells vs. B16-F10 cells, respectively.
Article Snippet:
Techniques: Microarray, Control, Expressing
Journal: PLoS ONE
Article Title: Low Dose Decitabine Treatment Induces CD80 Expression in Cancer Cells and Stimulates Tumor Specific Cytotoxic T Lymphocyte Responses
doi: 10.1371/journal.pone.0062924
Figure Lengend Snippet: The mouse SmartArray chips were hybridized with RNA derived from DAC or PBS treated EL4 cells. (A) Heatmap of some upregulated genes by DAC treatment. Columns represent microarray data obtained from 3 independent biological replicates. (B) RT-PCR was used to validate the up-regulated genes. (C) qRT-PCR was used to quantify up-regulated genes in DAC and PBS treated EL4 cells.
Article Snippet:
Techniques: Derivative Assay, Microarray, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR