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Illumina Inc smart oligos
Smart Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/smart oligos/product/Illumina Inc
Average 95 stars, based on 2 article reviews
Price from $9.99 to $1999.99
smart oligos - by Bioz Stars, 2020-04
95/100 stars

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Related Articles

RNA Sequencing Assay:

Article Title: Complement receptor CD46 co-stimulates optimal human CD8+ T cell effector function via fatty acid metabolism
Article Snippet: Paragraph title: RNA-Seq data generation and analysis ... Briefly, full-length cDNA synthesis was performed using oligo-dT and SMART oligos and the Illumina sequencing adaptors and barcodes incorporated into the final library by PCR.

Construct:

Article Title: Complement receptor CD46 co-stimulates optimal human CD8+ T cell effector function via fatty acid metabolism
Article Snippet: The sequencing libraries were constructed from 10 pg–10 ng of total RNA using the Takara Bio USA’s SMART-Seq V4 Ultra Low Input RNA Kit for Sequencing (Cat # 634888) following the manufacturer instruction. .. Briefly, full-length cDNA synthesis was performed using oligo-dT and SMART oligos and the Illumina sequencing adaptors and barcodes incorporated into the final library by PCR.

Sequencing:

Article Title: Complement receptor CD46 co-stimulates optimal human CD8+ T cell effector function via fatty acid metabolism
Article Snippet: .. Briefly, full-length cDNA synthesis was performed using oligo-dT and SMART oligos and the Illumina sequencing adaptors and barcodes incorporated into the final library by PCR. .. The fragment size of the RNAseq libraries was verified using the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA) and the concentrations determined using the Qubit instrument (Life Technologies, Carlsbad, CA, USA).

Generated:

Article Title: Complement receptor CD46 co-stimulates optimal human CD8+ T cell effector function via fatty acid metabolism
Article Snippet: Briefly, full-length cDNA synthesis was performed using oligo-dT and SMART oligos and the Illumina sequencing adaptors and barcodes incorporated into the final library by PCR. .. The libraries were loaded onto the Illumina HiSeq 3000 machine (Illumina, San Diego, CA, USA) for 1 × 50 bp single end read sequencing and generated about 55 M reads per sample.

Polymerase Chain Reaction:

Article Title: Complement receptor CD46 co-stimulates optimal human CD8+ T cell effector function via fatty acid metabolism
Article Snippet: .. Briefly, full-length cDNA synthesis was performed using oligo-dT and SMART oligos and the Illumina sequencing adaptors and barcodes incorporated into the final library by PCR. .. The fragment size of the RNAseq libraries was verified using the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA) and the concentrations determined using the Qubit instrument (Life Technologies, Carlsbad, CA, USA).

Software:

Article Title: Complement receptor CD46 co-stimulates optimal human CD8+ T cell effector function via fatty acid metabolism
Article Snippet: Briefly, full-length cDNA synthesis was performed using oligo-dT and SMART oligos and the Illumina sequencing adaptors and barcodes incorporated into the final library by PCR. .. The.fastq files were generated using the bcl2fastq software (Illumina) for further analysis.

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    Illumina Inc dna smart poly da primer
    Strand-specific amplification of non-targeted sequences at poly(T/A) sites in the <t>SMART</t> ChIP-seq analysis. a ). b , c Modified flowcharts to show annealing of the SMART poly(dA) primers to non-tailed Ts within targeted ( b ) or non-targeted ( c ) <t>DNA</t> templates, leading to strand-specific amplification at poly(T) sites. For poly(A) sites, false amplification occurs to the opposite strand. d - f ] is used to show the ChIP-seq reads from paired-end (PE) or single-end (SE) sequencing. For PE, read1 and read2 are shown as pairs, with reads mapped to “+” and “-“strands in red and blue, respectively. For SE, only Read1 (extracted from PE data) is shown. g - i : Fig. S6) for the calculation of read distribution
    Dna Smart Poly Da Primer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna smart poly da primer/product/Illumina Inc
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dna smart poly da primer - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    85
    Illumina Inc smart cdna primer end
    Strand-specific amplification of non-targeted sequences at poly(T/A) sites in the <t>SMART</t> ChIP-seq analysis. a ). b , c Modified flowcharts to show annealing of the SMART poly(dA) primers to non-tailed Ts within targeted ( b ) or non-targeted ( c ) <t>DNA</t> templates, leading to strand-specific amplification at poly(T) sites. For poly(A) sites, false amplification occurs to the opposite strand. d - f ] is used to show the ChIP-seq reads from paired-end (PE) or single-end (SE) sequencing. For PE, read1 and read2 are shown as pairs, with reads mapped to “+” and “-“strands in red and blue, respectively. For SE, only Read1 (extracted from PE data) is shown. g - i : Fig. S6) for the calculation of read distribution
    Smart Cdna Primer End, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smart cdna primer end/product/Illumina Inc
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    smart cdna primer end - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    93
    Illumina Inc smart pico oligos
    Strand-specific amplification of non-targeted sequences at poly(T/A) sites in the <t>SMART</t> ChIP-seq analysis. a ). b , c Modified flowcharts to show annealing of the SMART poly(dA) primers to non-tailed Ts within targeted ( b ) or non-targeted ( c ) <t>DNA</t> templates, leading to strand-specific amplification at poly(T) sites. For poly(A) sites, false amplification occurs to the opposite strand. d - f ] is used to show the ChIP-seq reads from paired-end (PE) or single-end (SE) sequencing. For PE, read1 and read2 are shown as pairs, with reads mapped to “+” and “-“strands in red and blue, respectively. For SE, only Read1 (extracted from PE data) is shown. g - i : Fig. S6) for the calculation of read distribution
    Smart Pico Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smart pico oligos/product/Illumina Inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    smart pico oligos - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Strand-specific amplification of non-targeted sequences at poly(T/A) sites in the SMART ChIP-seq analysis. a ). b , c Modified flowcharts to show annealing of the SMART poly(dA) primers to non-tailed Ts within targeted ( b ) or non-targeted ( c ) DNA templates, leading to strand-specific amplification at poly(T) sites. For poly(A) sites, false amplification occurs to the opposite strand. d - f ] is used to show the ChIP-seq reads from paired-end (PE) or single-end (SE) sequencing. For PE, read1 and read2 are shown as pairs, with reads mapped to “+” and “-“strands in red and blue, respectively. For SE, only Read1 (extracted from PE data) is shown. g - i : Fig. S6) for the calculation of read distribution

    Journal: BMC Bioinformatics

    Article Title: SMARTcleaner: identify and clean off-target signals in SMART ChIP-seq analysis

    doi: 10.1186/s12859-018-2577-4

    Figure Lengend Snippet: Strand-specific amplification of non-targeted sequences at poly(T/A) sites in the SMART ChIP-seq analysis. a ). b , c Modified flowcharts to show annealing of the SMART poly(dA) primers to non-tailed Ts within targeted ( b ) or non-targeted ( c ) DNA templates, leading to strand-specific amplification at poly(T) sites. For poly(A) sites, false amplification occurs to the opposite strand. d - f ] is used to show the ChIP-seq reads from paired-end (PE) or single-end (SE) sequencing. For PE, read1 and read2 are shown as pairs, with reads mapped to “+” and “-“strands in red and blue, respectively. For SE, only Read1 (extracted from PE data) is shown. g - i : Fig. S6) for the calculation of read distribution

    Article Snippet: When the SMART protocol (or kit) is applied to prepare NGS libraries from DNA fragments, such as those from chromatin immunoprecipitation (IP), there are five steps, 1) 3′ T-tailing, 2) annealing of DNA SMART poly(dA) primer to the T-tails, 3) primer extension by the SMARTScribe™ reverse transcriptase (RT), 4) template switching and extension by RT using SMART oligo, and 5) PCR-mediated addition of Illumina adapters and subsequent amplification (Fig. a).

    Techniques: Amplification, Chromatin Immunoprecipitation, Modification, Sequencing

    Strand-specific amplification of non-targeted sequences at poly(T/A) sites in the SMART ChIP-seq analysis. a Flowchart of the SMART ChIP-seq procedure at non-poly(T/A) sites, adapted from the user manual of the kit ( https://www.takarabio.com/documents/User%20Manual/DNA%20SMART%20ChIP-Seq%20Kit%20User%20Manual_101617.pdf ). b , c Modified flowcharts to show annealing of the SMART poly(dA) primers to non-tailed Ts within targeted ( b ) or non-targeted ( c ) DNA templates, leading to strand-specific amplification at poly(T) sites. For poly(A) sites, false amplification occurs to the opposite strand. d - f ChIP-seq read densities at three randomly picked non-poly(T/A) and poly(T/A) sites. The data is from SRR3229031 (Additional file 1 : Table S1, Dataset 1), and Integrative Genomics Viewer (IGV) [ 32 ] is used to show the ChIP-seq reads from paired-end (PE) or single-end (SE) sequencing. For PE, read1 and read2 are shown as pairs, with reads mapped to “+” and “-“strands in red and blue, respectively. For SE, only Read1 (extracted from PE data) is shown. g - i . Aggregated read distribution at non-poly(T/A) and poly(T/A) sites. In h and i, poly(T/A) sites were defined as those with ≥12 consecutive T or A in the human reference genome. To define non-poly(T/A) sites, we first selected genomic regions that are > 4 kb in length and > 1 kb away from poly(T/A) sites, and then take the 2 kb regions around the middle points. In total, we got 301,474 non-poly(T/A) sites, 338,568 poly(T) sites, and 336,703 poly(A) sites. Refer to the Method section (SE mode, Additional file 2 : Fig. S6) for the calculation of read distribution

    Journal: BMC Bioinformatics

    Article Title: SMARTcleaner: identify and clean off-target signals in SMART ChIP-seq analysis

    doi: 10.1186/s12859-018-2577-4

    Figure Lengend Snippet: Strand-specific amplification of non-targeted sequences at poly(T/A) sites in the SMART ChIP-seq analysis. a Flowchart of the SMART ChIP-seq procedure at non-poly(T/A) sites, adapted from the user manual of the kit ( https://www.takarabio.com/documents/User%20Manual/DNA%20SMART%20ChIP-Seq%20Kit%20User%20Manual_101617.pdf ). b , c Modified flowcharts to show annealing of the SMART poly(dA) primers to non-tailed Ts within targeted ( b ) or non-targeted ( c ) DNA templates, leading to strand-specific amplification at poly(T) sites. For poly(A) sites, false amplification occurs to the opposite strand. d - f ChIP-seq read densities at three randomly picked non-poly(T/A) and poly(T/A) sites. The data is from SRR3229031 (Additional file 1 : Table S1, Dataset 1), and Integrative Genomics Viewer (IGV) [ 32 ] is used to show the ChIP-seq reads from paired-end (PE) or single-end (SE) sequencing. For PE, read1 and read2 are shown as pairs, with reads mapped to “+” and “-“strands in red and blue, respectively. For SE, only Read1 (extracted from PE data) is shown. g - i . Aggregated read distribution at non-poly(T/A) and poly(T/A) sites. In h and i, poly(T/A) sites were defined as those with ≥12 consecutive T or A in the human reference genome. To define non-poly(T/A) sites, we first selected genomic regions that are > 4 kb in length and > 1 kb away from poly(T/A) sites, and then take the 2 kb regions around the middle points. In total, we got 301,474 non-poly(T/A) sites, 338,568 poly(T) sites, and 336,703 poly(A) sites. Refer to the Method section (SE mode, Additional file 2 : Fig. S6) for the calculation of read distribution

    Article Snippet: Strand-specific false priming and amplification at the poly(T/a) sites When the SMART protocol (or kit) is applied to prepare NGS libraries from DNA fragments, such as those from chromatin immunoprecipitation (IP), there are five steps, 1) 3′ T-tailing, 2) annealing of DNA SMART poly(dA) primer to the T-tails, 3) primer extension by the SMARTScribe™ reverse transcriptase (RT), 4) template switching and extension by RT using SMART oligo, and 5) PCR-mediated addition of Illumina adapters and subsequent amplification (Fig. a).

    Techniques: Amplification, Chromatin Immunoprecipitation, Modification, Sequencing