Journal: Journal of Agricultural and Food Chemistry

Article Title: Production of Xylooligosaccharides (XOSs) with Anti-inflammatory and Prebiotic Activities Using Recombinant Endo-1,4-β-xylanases from Bacillus subtilis

doi: 10.1021/acs.jafc.5c13633

Figure Lengend Snippet: Bioconversion of xylan to XOS by MBP- Bs XynA11 and Bs XynA11-His. TLC analysis of XOS converted by MBP- Bs XynA11 (A) and Bs XynA11-His (B) after 1–48 h conversion. XOS standards (X1–X6) and control (B = blank) are shown. The corresponding XOS bioconversion yield (%) and concentration (g/L) for MBP- B sXynA11 (C) and B sXynA11-His (D), based on HPLC quantification after 48 h. Columns represent yield, while concentration is shown as the lines. Profile of XOS released from beechwood xylan by MBP- B sXynA11 (E) and B sXynA11-His (F) under varying reaction conditions. Enzymatic hydrolysis was performed using beechwood xylan concentrations of 1, 2, and 4% (w/v) and enzyme doses of 500–1500 U/mL at 40 °C and 900 rpm in citrate–phosphate buffer (pH 6.5) for 48 h. The resulting XOSs were quantified by HPLC using xylose (X1), xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5), and xylohexaose (X6) as standards (0–1.67 g/L).

Article Snippet: Small-scale bioconversion of XOS was conducted in a 1.5 mL Eppendorf tube.

Techniques: Control, Concentration Assay

Journal: Journal of Agricultural and Food Chemistry

Article Title: Production of Xylooligosaccharides (XOSs) with Anti-inflammatory and Prebiotic Activities Using Recombinant Endo-1,4-β-xylanases from Bacillus subtilis

doi: 10.1021/acs.jafc.5c13633

Figure Lengend Snippet: Lab-scale bioconversion of XOS from beechwood xylan by MBP- B sXynA11. Physical appearance and representative chemical structures of beechwood xylan (A) and lyophilized XOS products (B) following enzymatic hydrolysis. (C) Time-course TLC analysis of XOS production at 0.5–48 h using MBP- Bs XynA11 (500 U/mL) at 40 °C, pH 6.5. Standards (X1–X6) and blank (B) included; (D) HPLC chromatogram of XOS products showing retention times of individual components: xylose (X1), xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5), xylohexaose (X6), and unquantified oligomers with DP > 6. Reactions were conducted in 100 mL volumes with 2% xylan at 250 rpm for 48 h.

Article Snippet: Small-scale bioconversion of XOS was conducted in a 1.5 mL Eppendorf tube.

Techniques:

Journal: bioRxiv

Article Title: DyGraphTrans: A temporal graph representation learning framework for modeling disease progression from Electronic Health Records

doi: 10.64898/2026.01.28.702347

Figure Lengend Snippet:

Article Snippet: We also evaluated our method four small-scale (DBLP-3, Brain, Reddit, and DBLP-10) and two large-scale (arXiv and Tmall) benchmark datasets.

Techniques: Comparison