small rna library prep kit  (Illumina Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    TruSeq RNA Library Prep
    Description:
    TruSeq RNA Exome previously known as the TruSeq RNA Access Library Prep Kit converts total RNA into template molecules of known strand origin followed by sequence specific capture of coding RNA This provides a low cost solution for analyzing human RNA isolated from formalin fixed paraffin embedded FFPE tissues and other low quality samples Affordability and focus Isolating human transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth High quality data from difficult samples Optimized for sequencing RNA from degraded samples including FFPE tissues Samples with limited starting material Greatly reduced sample input requirements as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples while maintaining high sensitivity TruSeq RNA Exome generates RNA sequencing RNA Seq libraries from degraded samples that focus on the RNA coding regions Isolating these high value content regions to help maximize discovery power while requiring only a fraction of the read depth of total RNA sequencing The results are low input requirements high sample throughput and cost effective transcriptome analysis Reagent volumes supplied are sufficient to support 1 to 4 plex enrichment reactions Find automation vendors with robotic systems compatible with this product
    Catalog Number:
    20020189
    Price:
    None
    Category:
    Library Preparation Kits
    Buy from Supplier


    Structured Review

    Illumina Inc small rna library prep kit
    TruSeq RNA Library Prep
    TruSeq RNA Exome previously known as the TruSeq RNA Access Library Prep Kit converts total RNA into template molecules of known strand origin followed by sequence specific capture of coding RNA This provides a low cost solution for analyzing human RNA isolated from formalin fixed paraffin embedded FFPE tissues and other low quality samples Affordability and focus Isolating human transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth High quality data from difficult samples Optimized for sequencing RNA from degraded samples including FFPE tissues Samples with limited starting material Greatly reduced sample input requirements as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples while maintaining high sensitivity TruSeq RNA Exome generates RNA sequencing RNA Seq libraries from degraded samples that focus on the RNA coding regions Isolating these high value content regions to help maximize discovery power while requiring only a fraction of the read depth of total RNA sequencing The results are low input requirements high sample throughput and cost effective transcriptome analysis Reagent volumes supplied are sufficient to support 1 to 4 plex enrichment reactions Find automation vendors with robotic systems compatible with this product
    https://www.bioz.com/result/small rna library prep kit/product/Illumina Inc
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    small rna library prep kit - by Bioz Stars, 2020-07
    99/100 stars

    Images

    Related Articles

    RNA Sequencing Assay:

    Article Title: TSS-EMOTE, a refined protocol for a more complete and less biased global mapping of transcription start sites in bacterial pathogens
    Article Snippet: .. Interestingly, we observe a slight shift of about 10 nt, between the rTSSs and the increase in coverage, which underlines that the TruSeq RNA sequencing protocol (used to prepare the RNAseq library for Illumina sequencing) does not preserve the native 5’-end of the RNA. ..

    Article Title: A comparative analysis of library prep approaches for sequencing low input translatome samples
    Article Snippet: .. We compared 5 methods of library preparation for Illumina Next Generation sequencing: NuGEN Ovation RNA-Seq system V2 Kit, TaKaRa SMARTer Stranded Total RNA-Seq Kit, TaKaRa SMART-Seq v4 Ultra Low Input RNA Kit, Illumina TruSeq RNA Library Prep Kit v2 and NEBNext® Ultra™ Directional RNA Library Prep Kit using slightly modified protocols each with 4 ng of total RNA. ..

    Article Title: A comparative analysis of library prep approaches for sequencing low input translatome samples
    Article Snippet: .. NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB) with 4 ng of RNA,NuGEN Ovation® RNA-Seq System V2 with 4 ng of RNA (NuGEN) with 4 ng of RNA, TaKaRa SMARTer® Stranded Total RNA-Seq Kit-Pico Input Mammalian with 4 ng of RNA (SMARTer), TaKaRa SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing with 4 ng and 0.25 ng of RNA (SMARTseq4 and SMARTseq0.25) and Illumina TruSeq RNA Library Prep Kit v2 with 4 ng and 70 ng of RNA (TruSeq4 and TruSeq70). .. Of these kits only the SMARTer kit produced strand specific libraries and we therefore did not analyze the data for strandness.

    Next-Generation Sequencing:

    Article Title: A comparative analysis of library prep approaches for sequencing low input translatome samples
    Article Snippet: .. We compared 5 methods of library preparation for Illumina Next Generation sequencing: NuGEN Ovation RNA-Seq system V2 Kit, TaKaRa SMARTer Stranded Total RNA-Seq Kit, TaKaRa SMART-Seq v4 Ultra Low Input RNA Kit, Illumina TruSeq RNA Library Prep Kit v2 and NEBNext® Ultra™ Directional RNA Library Prep Kit using slightly modified protocols each with 4 ng of total RNA. ..

    Sequencing:

    Article Title: A comprehensive assessment of RNA-seq protocols for degraded and low-quantity samples
    Article Snippet: .. Sequencing libraries Poly-A enriched strand-specific libraries were generated with the TruSeq mRNA V2 sample preparation kit (#RS-122-2001, Illumina), ribosomal RNA depleted strand-specific RNA libraries with the TruSeq Stranded Total RNA LT sample preparation kit with Ribo-Zero Gold (#RS-122-2301and (#RS-122-2302, Illumina), and transcriptome capture based libraries with the TruSeq RNA Access Library Prep Kit (#RS-301-2001, Illumina). ..

    Article Title: TSS-EMOTE, a refined protocol for a more complete and less biased global mapping of transcription start sites in bacterial pathogens
    Article Snippet: .. Interestingly, we observe a slight shift of about 10 nt, between the rTSSs and the increase in coverage, which underlines that the TruSeq RNA sequencing protocol (used to prepare the RNAseq library for Illumina sequencing) does not preserve the native 5’-end of the RNA. ..

    Article Title: Decreasing miRNA sequencing bias using a single adapter and circularization approach
    Article Snippet: .. Sequencing results of a library prepared using the TruSeq® Small RNA kit (Illumina) with 1 pmol of synthetic miRNAs (miRXplore™ Universal Pool) as input. .. Ligation and circularization efficiency of a single-adapter to the group of miRNAs selected in Figure S1.

    Article Title: A comparative analysis of library prep approaches for sequencing low input translatome samples
    Article Snippet: .. NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB) with 4 ng of RNA,NuGEN Ovation® RNA-Seq System V2 with 4 ng of RNA (NuGEN) with 4 ng of RNA, TaKaRa SMARTer® Stranded Total RNA-Seq Kit-Pico Input Mammalian with 4 ng of RNA (SMARTer), TaKaRa SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing with 4 ng and 0.25 ng of RNA (SMARTseq4 and SMARTseq0.25) and Illumina TruSeq RNA Library Prep Kit v2 with 4 ng and 70 ng of RNA (TruSeq4 and TruSeq70). .. Of these kits only the SMARTer kit produced strand specific libraries and we therefore did not analyze the data for strandness.

    Article Title: Targeted Sequencing of Respiratory Viruses in Clinical Specimens for Pathogen Identification and Genome-Wide Analysis
    Article Snippet: .. Illumina TruSeq RNA Access Library procedure is used in targeted sequencing of respiratory viruses. .. The samples are subjected to RNA fragmentation, random reverse transcription, random PCR amplification, and ligation with barcoded library adaptors.

    Generated:

    Article Title: A comprehensive assessment of RNA-seq protocols for degraded and low-quantity samples
    Article Snippet: .. Sequencing libraries Poly-A enriched strand-specific libraries were generated with the TruSeq mRNA V2 sample preparation kit (#RS-122-2001, Illumina), ribosomal RNA depleted strand-specific RNA libraries with the TruSeq Stranded Total RNA LT sample preparation kit with Ribo-Zero Gold (#RS-122-2301and (#RS-122-2302, Illumina), and transcriptome capture based libraries with the TruSeq RNA Access Library Prep Kit (#RS-301-2001, Illumina). ..

    Modification:

    Article Title: A comparative analysis of library prep approaches for sequencing low input translatome samples
    Article Snippet: .. We compared 5 methods of library preparation for Illumina Next Generation sequencing: NuGEN Ovation RNA-Seq system V2 Kit, TaKaRa SMARTer Stranded Total RNA-Seq Kit, TaKaRa SMART-Seq v4 Ultra Low Input RNA Kit, Illumina TruSeq RNA Library Prep Kit v2 and NEBNext® Ultra™ Directional RNA Library Prep Kit using slightly modified protocols each with 4 ng of total RNA. ..

    Sample Prep:

    Article Title: A comprehensive assessment of RNA-seq protocols for degraded and low-quantity samples
    Article Snippet: .. Sequencing libraries Poly-A enriched strand-specific libraries were generated with the TruSeq mRNA V2 sample preparation kit (#RS-122-2001, Illumina), ribosomal RNA depleted strand-specific RNA libraries with the TruSeq Stranded Total RNA LT sample preparation kit with Ribo-Zero Gold (#RS-122-2301and (#RS-122-2302, Illumina), and transcriptome capture based libraries with the TruSeq RNA Access Library Prep Kit (#RS-301-2001, Illumina). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Illumina Inc nebnextmultiplex small rna library prep
    Schematic pipeline illustrating the workflow of nephron progenitor isolation and bioinformatics analysis of the small <t>RNA-Seq</t> dataset. For nephron progenitor isolation, embryonic day 15.5 (E15.5) CD1 mouse kidneys were subjected to limited digestion, followed by negative cell selection through Magnetic Activated Cell Sorting (MACS). Total RNA from the isolate was extracted and subjected to qPCR analysis for enrichment of nephron progenitor markers. Following verification of nephron progenitor enrichment, total RNA was used as an input for the <t>NEBNextMultiplex</t> Small RNA Library Prep to generate libraries for the sRNA-Seq. The sRNA-Seq dataset was analysed in accordance with the pipeline, with the fastq files first analysed by FastQC to determine the quality of the sequencing reads, followed by adaptor removal using the BBDuk package, and finally aligned, quantified and annotated to the mus musculus mm10 genome using the miRDeep2 package.
    Nebnextmultiplex Small Rna Library Prep, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnextmultiplex small rna library prep/product/Illumina Inc
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nebnextmultiplex small rna library prep - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    93
    Illumina Inc srna libraries
    Small <t>RNA</t> expression profiles detected with RNA-seq. The panels include the known sRNAs GlmZ, CyaR, MicF, McaS and RyhB, a novel <t>sRNA</t> with clearly defined coordinates, ES043, a novel sRNA with less defined coordinates, ES067, and a novel sRNA with vaguely defined coordinates and low expression level, ES116. The y-axes of the plots denote read coverage at each nucleotide position for the pooled fermentation and stress samples. The x-axes denote the genomic positions according to the E. coli K-12 MG1655 genome coordinates (NC_000913.2). Legend: orange lines, expression signal; dashed green lines, sRNA coordinates; dashed blue lines, intergenic region coordinates; arrows denote directions of flanking genes (blue) and sRNAs (green)
    Srna Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/srna libraries/product/Illumina Inc
    Average 93 stars, based on 160 article reviews
    Price from $9.99 to $1999.99
    srna libraries - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    86
    Illumina Inc srna sample preparation kit
    Northern blot analysis of novel sRNAs. (A) A 20 μg sample of <t>RNA</t> was analyzed via Northern Blot analysis to confirm expression and size of putative sRNAs. All putative sRNAs that were examined were confirmed. <t>sRNA</t> expression was also tested under conditions related to those used for RNA-seq. Growth for 90 min under iron replete conditions (indicated by “+”), and growth for 90 min under iron deplete conditions (indicated by “−”). Experiments were performed three times and a representative film is shown. (B) Table showing previously known non-coding sRNAs that were also found in this study.
    Srna Sample Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/srna sample preparation kit/product/Illumina Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    srna sample preparation kit - by Bioz Stars, 2020-07
    86/100 stars
      Buy from Supplier

    86
    Illumina Inc small noncoding rna high throughput sequencing small rnas
    Small <t>RNA</t> annotations for mouse ESCs, SSCs, MSCs, STs, and GCs. The pie chart on the left for each cell type indicates the relative frequency of the annotated <t>noncoding</t> <t>RNAs,</t> and the right panel shows the absolute number of reads annotated.
    Small Noncoding Rna High Throughput Sequencing Small Rnas, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/small noncoding rna high throughput sequencing small rnas/product/Illumina Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    small noncoding rna high throughput sequencing small rnas - by Bioz Stars, 2020-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Schematic pipeline illustrating the workflow of nephron progenitor isolation and bioinformatics analysis of the small RNA-Seq dataset. For nephron progenitor isolation, embryonic day 15.5 (E15.5) CD1 mouse kidneys were subjected to limited digestion, followed by negative cell selection through Magnetic Activated Cell Sorting (MACS). Total RNA from the isolate was extracted and subjected to qPCR analysis for enrichment of nephron progenitor markers. Following verification of nephron progenitor enrichment, total RNA was used as an input for the NEBNextMultiplex Small RNA Library Prep to generate libraries for the sRNA-Seq. The sRNA-Seq dataset was analysed in accordance with the pipeline, with the fastq files first analysed by FastQC to determine the quality of the sequencing reads, followed by adaptor removal using the BBDuk package, and finally aligned, quantified and annotated to the mus musculus mm10 genome using the miRDeep2 package.

    Journal: Scientific Data

    Article Title: Small non-coding RNA expression in mouse nephrogenic mesenchymal progenitors

    doi: 10.1038/sdata.2018.218

    Figure Lengend Snippet: Schematic pipeline illustrating the workflow of nephron progenitor isolation and bioinformatics analysis of the small RNA-Seq dataset. For nephron progenitor isolation, embryonic day 15.5 (E15.5) CD1 mouse kidneys were subjected to limited digestion, followed by negative cell selection through Magnetic Activated Cell Sorting (MACS). Total RNA from the isolate was extracted and subjected to qPCR analysis for enrichment of nephron progenitor markers. Following verification of nephron progenitor enrichment, total RNA was used as an input for the NEBNextMultiplex Small RNA Library Prep to generate libraries for the sRNA-Seq. The sRNA-Seq dataset was analysed in accordance with the pipeline, with the fastq files first analysed by FastQC to determine the quality of the sequencing reads, followed by adaptor removal using the BBDuk package, and finally aligned, quantified and annotated to the mus musculus mm10 genome using the miRDeep2 package.

    Article Snippet: The NEBNextMultiplex Small RNA Library Prep Set for Illumina (NEB; #E7300S) was used to synthesise barcoded cDNA libraries from 100 ng total RNA in accordance with the manufacturer’s protocol and size selection performed using the Agencourt AMPure XP beads (Beckman Coulter; #A63881).

    Techniques: Isolation, RNA Sequencing Assay, Selection, FACS, Magnetic Cell Separation, Real-time Polymerase Chain Reaction, Sequencing

    Small RNA expression profiles detected with RNA-seq. The panels include the known sRNAs GlmZ, CyaR, MicF, McaS and RyhB, a novel sRNA with clearly defined coordinates, ES043, a novel sRNA with less defined coordinates, ES067, and a novel sRNA with vaguely defined coordinates and low expression level, ES116. The y-axes of the plots denote read coverage at each nucleotide position for the pooled fermentation and stress samples. The x-axes denote the genomic positions according to the E. coli K-12 MG1655 genome coordinates (NC_000913.2). Legend: orange lines, expression signal; dashed green lines, sRNA coordinates; dashed blue lines, intergenic region coordinates; arrows denote directions of flanking genes (blue) and sRNAs (green)

    Journal: BMC Genomics

    Article Title: Differential expression of small RNAs under chemical stress and fed-batch fermentation in E. coli

    doi: 10.1186/s12864-015-2231-8

    Figure Lengend Snippet: Small RNA expression profiles detected with RNA-seq. The panels include the known sRNAs GlmZ, CyaR, MicF, McaS and RyhB, a novel sRNA with clearly defined coordinates, ES043, a novel sRNA with less defined coordinates, ES067, and a novel sRNA with vaguely defined coordinates and low expression level, ES116. The y-axes of the plots denote read coverage at each nucleotide position for the pooled fermentation and stress samples. The x-axes denote the genomic positions according to the E. coli K-12 MG1655 genome coordinates (NC_000913.2). Legend: orange lines, expression signal; dashed green lines, sRNA coordinates; dashed blue lines, intergenic region coordinates; arrows denote directions of flanking genes (blue) and sRNAs (green)

    Article Snippet: Finally sRNA libraries were constructed using the TruSeq Small RNA Sample Preparation Kit (Illumina) with a few modifications.

    Techniques: RNA Expression, RNA Sequencing Assay, Expressing

    Features of the 253 novel small RNA transcripts detected in this study in relation to previously described sRNAs. Distribution of small RNAs on the E. coli MG1655 genome ( a ), where the novel sRNAs detected in this study are depicted on the inner ring, the known annotated sRNAs on the middle ring and the sRNAs detected in [ 17 ] on the outer ring. The transcript lengths and expression levels for the novel small RNAs compared to the known annotated and those predicted by Shinhara et al. [ 17 ], are shown in ( b ) and ( c ), respectively. Expression levels are presented as cumulative Mean Expression Values (MEV) defined as total stress and fermentation library reads for each sRNA divided by sRNA length

    Journal: BMC Genomics

    Article Title: Differential expression of small RNAs under chemical stress and fed-batch fermentation in E. coli

    doi: 10.1186/s12864-015-2231-8

    Figure Lengend Snippet: Features of the 253 novel small RNA transcripts detected in this study in relation to previously described sRNAs. Distribution of small RNAs on the E. coli MG1655 genome ( a ), where the novel sRNAs detected in this study are depicted on the inner ring, the known annotated sRNAs on the middle ring and the sRNAs detected in [ 17 ] on the outer ring. The transcript lengths and expression levels for the novel small RNAs compared to the known annotated and those predicted by Shinhara et al. [ 17 ], are shown in ( b ) and ( c ), respectively. Expression levels are presented as cumulative Mean Expression Values (MEV) defined as total stress and fermentation library reads for each sRNA divided by sRNA length

    Article Snippet: Finally sRNA libraries were constructed using the TruSeq Small RNA Sample Preparation Kit (Illumina) with a few modifications.

    Techniques: Expressing

    Northern blot analysis of novel sRNAs. (A) A 20 μg sample of RNA was analyzed via Northern Blot analysis to confirm expression and size of putative sRNAs. All putative sRNAs that were examined were confirmed. sRNA expression was also tested under conditions related to those used for RNA-seq. Growth for 90 min under iron replete conditions (indicated by “+”), and growth for 90 min under iron deplete conditions (indicated by “−”). Experiments were performed three times and a representative film is shown. (B) Table showing previously known non-coding sRNAs that were also found in this study.

    Journal: Frontiers in Microbiology

    Article Title: Identification of sRNAs expressed by the human pathogen Neisseria gonorrhoeae under disparate growth conditions

    doi: 10.3389/fmicb.2014.00456

    Figure Lengend Snippet: Northern blot analysis of novel sRNAs. (A) A 20 μg sample of RNA was analyzed via Northern Blot analysis to confirm expression and size of putative sRNAs. All putative sRNAs that were examined were confirmed. sRNA expression was also tested under conditions related to those used for RNA-seq. Growth for 90 min under iron replete conditions (indicated by “+”), and growth for 90 min under iron deplete conditions (indicated by “−”). Experiments were performed three times and a representative film is shown. (B) Table showing previously known non-coding sRNAs that were also found in this study.

    Article Snippet: A cDNA library was then generated from the enriched RNA using Illumina's sRNA Sample Preparation Kit and samples were sequenced on an Illumina GAIIx without barcoding.

    Techniques: Northern Blot, Expressing, RNA Sequencing Assay

    Small RNA annotations for mouse ESCs, SSCs, MSCs, STs, and GCs. The pie chart on the left for each cell type indicates the relative frequency of the annotated noncoding RNAs, and the right panel shows the absolute number of reads annotated.

    Journal: BioMed Research International

    Article Title: miRNA Signature in Mouse Spermatogonial Stem Cells Revealed by High-Throughput Sequencing

    doi: 10.1155/2014/154251

    Figure Lengend Snippet: Small RNA annotations for mouse ESCs, SSCs, MSCs, STs, and GCs. The pie chart on the left for each cell type indicates the relative frequency of the annotated noncoding RNAs, and the right panel shows the absolute number of reads annotated.

    Article Snippet: Overview of Small Noncoding RNA High-Throughput Sequencing Small RNAs were separated on 15% denaturing polyacrylamide gels and fragments of 18–40 nt were extracted and purified and used to construct a cDNA library, using the Illumina small RNA sample preparation kit (Illumina, San Diego, CA, USA).

    Techniques: