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Illumina Inc small rna library prep kit
Small Rna Library Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/small rna library prep kit/product/Illumina Inc
Average 88 stars, based on 2 article reviews
Price from $9.99 to $1999.99
small rna library prep kit - by Bioz Stars, 2019-12
88/100 stars

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Multiplex Assay:

Article Title: Circulating Extracellular RNA Markers of Liver Regeneration
Article Snippet: Purified exRNA was made into Illumina Tru-Seq RNA-Sequencing libraries, and samples were indexed using the NEBNext Small RNA Library prep kit (Illumina, San Diego, CA). .. The size and concentration of the purified libraries were analyzed using an Agilent 2100 Bioanalyzer high sensitivity DNA chip.

Sequencing:

Article Title: Analyses of RNA-Seq and sRNA-Seq data reveal a complex network of anti-viral defense in TCV-infected Arabidopsis thaliana
Article Snippet: Paragraph title: Small RNA libraries construction, sequencing and data analysis ... After quality control tests, the small RNA libraries were constructed by Small RNA Library Prep Kit for Illumina.

Article Title: Diversity and signature of small RNA in different bodily fluids using next generation sequencing
Article Snippet: Paragraph title: Small RNA library construction and high-throughput sequencing ... The small RNA libraries were prepared from the RNA isolated from each sample using the Small RNA Library Prep Kit for Illumina (Norgen Biotek Corp., Canada) according to the manufacturer’s instructions.

Article Title: Circulating Extracellular RNA Markers of Liver Regeneration
Article Snippet: Purified exRNA was made into Illumina Tru-Seq RNA-Sequencing libraries, and samples were indexed using the NEBNext Small RNA Library prep kit (Illumina, San Diego, CA). .. The size and concentration of the purified libraries were analyzed using an Agilent 2100 Bioanalyzer high sensitivity DNA chip.

Article Title: The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline
Article Snippet: Paragraph title: Small RNA library production and deep sequencing ... Small RNA libraries from the cn bw control line, and from cuff wm25 homozygous ovaries were produced with the Small RNA Library Prep Kit (Illumina).

RNA Sequencing Assay:

Article Title: Circulating Extracellular RNA Markers of Liver Regeneration
Article Snippet: We considered changes occurring within the first 6 hours as indicating early stages of hepatic regeneration after partial hepatectomy. .. Purified exRNA was made into Illumina Tru-Seq RNA-Sequencing libraries, and samples were indexed using the NEBNext Small RNA Library prep kit (Illumina, San Diego, CA). .. Amplified libraries were sized-fractionated using Sage Bioscience Pippin prep station on 2% agarose gels (Sage Bio cat # SDF2010) with a collection range of 135 bp—200 bp.

RNA HS Assay:

Article Title: Genome-scale analysis of syngas fermenting acetogenic bacteria reveals the translational regulation for its autotrophic growth
Article Snippet: After purification of the RNA samples using an RNeasy MinElute Column (Qiagen), the concentration of purified RNA was measured using the Qubit RNA HS assay kit (Invitrogen). .. For library construction, the small RNA library prep kit for Illumina (NEB) was used and the constructed library was sequenced using the 50 bp read recipe on an Illumina Hiseq2500.

Isolation:

Article Title: Diversity and signature of small RNA in different bodily fluids using next generation sequencing
Article Snippet: RNA concentration was then estimated by the Agilent 2100 Bioanalyzer System (Agilent Technologies, USA) using the RNA 6000 Nano Total RNA chip. .. The small RNA libraries were prepared from the RNA isolated from each sample using the Small RNA Library Prep Kit for Illumina (Norgen Biotek Corp., Canada) according to the manufacturer’s instructions. .. Briefly, 6 μL of purified RNA was mixed with the 3′ adapter and incubated at 70 °C for 2 min before being used in a ligation step by adding T4 RNA ligase 2 (truncated), buffer and RNase inhibitor.

Article Title: Genome-scale analysis of syngas fermenting acetogenic bacteria reveals the translational regulation for its autotrophic growth
Article Snippet: The recovered ribosome-bound RNA was isolated by using TRIzol and the remaining rRNAs were removed with the Ribo-Zero™ rRNA Removal Kit for Meta-bacteria. .. For library construction, the small RNA library prep kit for Illumina (NEB) was used and the constructed library was sequenced using the 50 bp read recipe on an Illumina Hiseq2500.

Construct:

Article Title: Analyses of RNA-Seq and sRNA-Seq data reveal a complex network of anti-viral defense in TCV-infected Arabidopsis thaliana
Article Snippet: Differentially expressed genes were acquired by DESeq2 ; the unmapped BAM files were converted to Fastq files via bedtools and aligned to virus reference genome by Bowtie 2 . .. After quality control tests, the small RNA libraries were constructed by Small RNA Library Prep Kit for Illumina. .. Single-end sequencing was performed on Illumina HiSeq 2000 with read length of 50 bp.

Article Title: Genome-scale analysis of syngas fermenting acetogenic bacteria reveals the translational regulation for its autotrophic growth
Article Snippet: After purification of the RNA samples using an RNeasy MinElute Column (Qiagen), the concentration of purified RNA was measured using the Qubit RNA HS assay kit (Invitrogen). .. For library construction, the small RNA library prep kit for Illumina (NEB) was used and the constructed library was sequenced using the 50 bp read recipe on an Illumina Hiseq2500. .. The sequence reads were trimmed to remove the adapter sequences.

Purification:

Article Title: Circulating Extracellular RNA Markers of Liver Regeneration
Article Snippet: We considered changes occurring within the first 6 hours as indicating early stages of hepatic regeneration after partial hepatectomy. .. Purified exRNA was made into Illumina Tru-Seq RNA-Sequencing libraries, and samples were indexed using the NEBNext Small RNA Library prep kit (Illumina, San Diego, CA). .. Amplified libraries were sized-fractionated using Sage Bioscience Pippin prep station on 2% agarose gels (Sage Bio cat # SDF2010) with a collection range of 135 bp—200 bp.

Article Title: Genome-scale analysis of syngas fermenting acetogenic bacteria reveals the translational regulation for its autotrophic growth
Article Snippet: After purification of the RNA samples using an RNeasy MinElute Column (Qiagen), the concentration of purified RNA was measured using the Qubit RNA HS assay kit (Invitrogen). .. For library construction, the small RNA library prep kit for Illumina (NEB) was used and the constructed library was sequenced using the 50 bp read recipe on an Illumina Hiseq2500.

Produced:

Article Title: The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline
Article Snippet: Primer pairs adopted in this assay are described in . .. Small RNA libraries from the cn bw control line, and from cuff wm25 homozygous ovaries were produced with the Small RNA Library Prep Kit (Illumina). .. Briefly, 20 μg total RNA extracted from the different genotypes was separated on a 15% denaturing polyacrylamide/urea gel electrophoresis (Invitrogen) for 1 h at 200 V. A gel slice corresponding to 18–30-nt-long RNAs was isolated and small RNAs were eluted and processed with the Small RNA Library Prep Kit as per the manufacturer's instructions.

Concentration Assay:

Article Title: Circulating Extracellular RNA Markers of Liver Regeneration
Article Snippet: Purified exRNA was made into Illumina Tru-Seq RNA-Sequencing libraries, and samples were indexed using the NEBNext Small RNA Library prep kit (Illumina, San Diego, CA). .. Amplified libraries were sized-fractionated using Sage Bioscience Pippin prep station on 2% agarose gels (Sage Bio cat # SDF2010) with a collection range of 135 bp—200 bp.

Article Title: Genome-scale analysis of syngas fermenting acetogenic bacteria reveals the translational regulation for its autotrophic growth
Article Snippet: After purification of the RNA samples using an RNeasy MinElute Column (Qiagen), the concentration of purified RNA was measured using the Qubit RNA HS assay kit (Invitrogen). .. For library construction, the small RNA library prep kit for Illumina (NEB) was used and the constructed library was sequenced using the 50 bp read recipe on an Illumina Hiseq2500.

Incubation:

Article Title: Diversity and signature of small RNA in different bodily fluids using next generation sequencing
Article Snippet: The small RNA libraries were prepared from the RNA isolated from each sample using the Small RNA Library Prep Kit for Illumina (Norgen Biotek Corp., Canada) according to the manufacturer’s instructions. .. The small RNA libraries were prepared from the RNA isolated from each sample using the Small RNA Library Prep Kit for Illumina (Norgen Biotek Corp., Canada) according to the manufacturer’s instructions.

Article Title: Genome-scale analysis of syngas fermenting acetogenic bacteria reveals the translational regulation for its autotrophic growth
Article Snippet: For the phosphorylation reaction, samples were denatured at 80 °C for 90 s, equilibrated to 37 °C, and incubated at 37 °C for 1 h with 5 μL of 10× T4 PNK buffer (NEB), 20 U SUPERase-In RNase Inhibitor, and 10 U T4 PNK (NEB). .. For library construction, the small RNA library prep kit for Illumina (NEB) was used and the constructed library was sequenced using the 50 bp read recipe on an Illumina Hiseq2500.

High Throughput Screening Assay:

Article Title: Diversity and signature of small RNA in different bodily fluids using next generation sequencing
Article Snippet: Paragraph title: Small RNA library construction and high-throughput sequencing ... The small RNA libraries were prepared from the RNA isolated from each sample using the Small RNA Library Prep Kit for Illumina (Norgen Biotek Corp., Canada) according to the manufacturer’s instructions.

Chromatin Immunoprecipitation:

Article Title: Circulating Extracellular RNA Markers of Liver Regeneration
Article Snippet: Purified exRNA was made into Illumina Tru-Seq RNA-Sequencing libraries, and samples were indexed using the NEBNext Small RNA Library prep kit (Illumina, San Diego, CA). .. Amplified libraries were sized-fractionated using Sage Bioscience Pippin prep station on 2% agarose gels (Sage Bio cat # SDF2010) with a collection range of 135 bp—200 bp.

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    Illumina Inc small rna library prep set kit
    Scatter plot of <t>RNA-seq</t> (A) and miRNA-seq (B) from control and MeHg (10µM) treated animals. <t>RNAs</t> were isolated from whole animals treated from embryo to L4 stage and libraries prepared and sequenced as described in Methods. Data were plotted as
    Small Rna Library Prep Set Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/small rna library prep set kit/product/Illumina Inc
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    small rna library prep set kit - by Bioz Stars, 2019-12
    77/100 stars
      Buy from Supplier

    80
    Illumina Inc directional rna seq truseq small rna sample prep kit
    Scatter plot of <t>RNA-seq</t> (A) and miRNA-seq (B) from control and MeHg (10µM) treated animals. <t>RNAs</t> were isolated from whole animals treated from embryo to L4 stage and libraries prepared and sequenced as described in Methods. Data were plotted as
    Directional Rna Seq Truseq Small Rna Sample Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 80/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/directional rna seq truseq small rna sample prep kit/product/Illumina Inc
    Average 80 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    directional rna seq truseq small rna sample prep kit - by Bioz Stars, 2019-12
    80/100 stars
      Buy from Supplier

    Image Search Results


    Scatter plot of RNA-seq (A) and miRNA-seq (B) from control and MeHg (10µM) treated animals. RNAs were isolated from whole animals treated from embryo to L4 stage and libraries prepared and sequenced as described in Methods. Data were plotted as

    Journal: Toxicology letters

    Article Title: Methylmercury exposure increases lipocalin related (lpr) and decreases activated in blocked unfolded protein response (abu) genes and specific miRNAs in Caenorhabditis elegans

    doi: 10.1016/j.toxlet.2013.07.014

    Figure Lengend Snippet: Scatter plot of RNA-seq (A) and miRNA-seq (B) from control and MeHg (10µM) treated animals. RNAs were isolated from whole animals treated from embryo to L4 stage and libraries prepared and sequenced as described in Methods. Data were plotted as

    Article Snippet: One µg of isolated small RNAs were used to construct a library using the Small RNA library prep set kit for Illumina (New England Biolabs, Ipswich, MA, USA) using the same conditions as reported previously ( ).

    Techniques: RNA Sequencing Assay, Isolation