Small Molecule Ml327 Modulates E Cadherin Mrna Levels, supplied by Novoprotein, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/small molecule ml327 modulates e cadherin mrna levels/product/Novoprotein
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
1) Product Images from "Small molecule/ML327 mediated transcriptional de-repression of E-cadherin and inhibition of epithelial-to-mesenchymal transition"
Article Title: Small molecule/ML327 mediated transcriptional de-repression of E-cadherin and inhibition of epithelial-to-mesenchymal transition
Figure Legend Snippet: Treatment with ML327 partially reverses TGF-β-induced EMT A. Bright field microcopy images (200x magnification) showing the cell morphology after 72 hours TGF- β1 treatment. B. Immunofluorescence images showing E-cadherin (red) expression and localization in NMuMg cells following treatment without or with 5ng/mL TGF-β1 for 72 hours, then adding either DMSO, or 10 μM ML327 for an additional 48 hours. Nuclei (blue) are labeled with DAPI. Bar = 100 μM. C. Relative levels of E-cadherin, Occludin and Vimentin specific mRNA species in NMuMg cells following treatments shown in B. , the graph shows mean values with standard error bars from 4 replicate wells in a representative experiment. Statistical significance was calculated using unpaired t test **** indicates p
Techniques Used: Immunofluorescence, Expressing, Labeling
Figure Legend Snippet: Treatment with ML327 inhibits tumor cell migration in vivo A. Relative E-cadherin mRNA expression in HEp3 cells following 6 hours treatment with either 266Y or ML327, the graph shows mean values with standard error bars from 3 replicate wells in a representative experiment, statistical significance was calculated using unpaired t test, *** indicates p
Techniques Used: Migration, In Vivo, Expressing
Figure Legend Snippet: ML327 increases E-cadherin expression in SW620inv colon cancer cells A. Quantitative PCR results for E-cadherin specific mRNA in SW620inv cells following treatment with ML327 at the indicated time periods (data points represent results from3 biological replicates). Fold change relative to DMSO treatment is determined by the formula log2 −ΔΔCp . B. Western blot shows time dependent changes in SW620inv E-cadherin (E-cad) protein expression relative to β-actin following treatment with DMSO, 10 μM ML327, or 266Y. C. Quantitative PCR analysis of E-cadherin mRNA in SW620inv cells following 1 hour with or without CHX pretreatment, then adding DMSO, ML327, or 266Y for another 6 hours. Fold change in E-cadherin mRNA, relative to DMSO without CHX is calculated by the formula log2 −ΔΔCp . Graphed data represent four independent experiments. D. ICW plate matched to C. showing quantification of cyclin D1 protein (green) and β-actin (red). E. The graph shows quantification of relative cyclin D1 signal matched to D. .
Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Western Blot