small hairpin rna shrna lentiviral particles  (Santa Cruz Biotechnology)

 
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    Name:
    Hip shRNA m Lentiviral Particles
    Description:
    Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of Hhip gene silencing results individual duplex components or plasmids are also available upon request Suitable control antibody Hhip Antibody 5D11 sc 293265 is recommended as control antibody for monitoring of Hhip expression knockdown by Western blotting or immunofluorescence
    Catalog Number:
    SC-40164-V
    Price:
    None
    Category:
    Gene Editing siRNA shRNA Gene Silencers Membrane Receptor Hhip siRNA shRNA Plasmid and Lentiviral Particle Gene Silencers Hhip siRNA and shRNA Plasmids m
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    Structured Review

    Santa Cruz Biotechnology small hairpin rna shrna lentiviral particles
    Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of Hhip gene silencing results individual duplex components or plasmids are also available upon request Suitable control antibody Hhip Antibody 5D11 sc 293265 is recommended as control antibody for monitoring of Hhip expression knockdown by Western blotting or immunofluorescence
    https://www.bioz.com/result/small hairpin rna shrna lentiviral particles/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    small hairpin rna shrna lentiviral particles - by Bioz Stars, 2021-07
    85/100 stars

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    shRNA:

    Article Title: ST13, a proliferation regulator, inhibits growth and migration of colorectal cancer cell lines *
    Article Snippet: The relative gene expression levels were calculated using the comparative threshold cycle C t (ΔΔ C t ) method (according to Applied Biosystems), where the relative expression is calculated as 2−ΔΔC t . .. Small hairpin RNA (shRNA) lentiviral particles used for ST13 knockdown (sc-40684-v) and Mock knockdown (sc-108080) were purchased from Santa Cruz, CA, USA. .. SW620 cells were infected with shRNA over 48 h in the presence of polybrene (4 μg/ml) in a 6-well plate as described previously (Shi et al., ).

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    Santa Cruz Biotechnology ahr shrna lentiviral particles
    CYP1B1 expression is regulated by constitutively active/nuclear <t>AhR.(A)</t> Confirmation of AhR protein expression in C4-2 cells in comparison to scrambled vector control (SCR) and <t>shRNA</t> lentivirus against AhR (-AhR) by western blotting. 20 μg of
    Ahr Shrna Lentiviral Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ahr shrna lentiviral particles/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    93
    Santa Cruz Biotechnology gli1
    The results of Human Stem Cell Signaling RT 2 Profiler PCR Array analysis indicated that As 2 O 3 down-regulated the expression of <t>GLI1</t> and PTCH1 in CD133 + Huh7-wt cells.
    Gli1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gli1/product/Santa Cruz Biotechnology
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    85
    Santa Cruz Biotechnology trek 1 shrna lentiviral particles
    <t>Trek-1</t> deficiency increases MLE-12 cell proliferation. A : increase in absolute cell numbers in Trek-1 <t>shRNA-transfected</t> cells after 48 h of culture at room air ( P
    Trek 1 Shrna Lentiviral Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trek 1 shrna lentiviral particles/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    91
    Santa Cruz Biotechnology lentiviral vectors targeting tmem16f
    Ebola virus (EBOV)–induced extracellular expression of phosphatidylserine (PtdSer) is dependent on transmembrane protein 16F <t>(TMEM16F)</t> function. Huh7 cells were transduced with <t>lentiviral</t> vectors encoding scrambled short hairpin RNA (shRNA) (Scr) or shRNAs specific for TMEM16F and infected with EBOV at a multiplicity of infection of 1 plaque-forming unit/cell. A, B, Translocation of PtdSer to the outer leaflet of the plasma membrane in EBOV-infected Huh7/shScramble ( A ) or Huh7/shTMEM16F ( B ) cells. Targeted knockdown of TMEM16F results in decreased levels of plasma membrane-associated PtdSer (scale bar represents 30 μm). C–E, Imaging flow cytometry analysis of mock-infected and EBOV-infected shRNA cell lines. C, Representative images of mock-infected and EBOV-infected Huh7/shScramble and Huh7/shTMEM16F cell lines. The merged image is based on glycoprotein (GP), annexin V, and 4′,6-diamidino-2-phenylindole (DAPI). D, Relative levels of EBOV GP ( left ) and annexin V staining ( right for gating strategy. Panels A and B are representative of 1 of 7 and panels C and D are representative of 1 of 3 independent experiments. Abbreviations: BF, bright field; SSC, side scatter.
    Lentiviral Vectors Targeting Tmem16f, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 1 article reviews
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    CYP1B1 expression is regulated by constitutively active/nuclear AhR.(A) Confirmation of AhR protein expression in C4-2 cells in comparison to scrambled vector control (SCR) and shRNA lentivirus against AhR (-AhR) by western blotting. 20 μg of

    Journal: Biochemical pharmacology

    Article Title: Inhibition of constitutive aryl hydrocarbon receptor (AhR) signaling attenuates androgen independent signaling and growth in (C4-2) prostate cancer cells

    doi: 10.1016/j.bcp.2012.12.010

    Figure Lengend Snippet: CYP1B1 expression is regulated by constitutively active/nuclear AhR.(A) Confirmation of AhR protein expression in C4-2 cells in comparison to scrambled vector control (SCR) and shRNA lentivirus against AhR (-AhR) by western blotting. 20 μg of

    Article Snippet: AhR shRNA lentiviral particles (Santa Cruz) were mixed with culture media and added to the C4-2 cells in the presence of polybrene (4 μg/ml) with gentle and thorough mixing.

    Techniques: Expressing, Plasmid Preparation, shRNA, Western Blot

    The results of Human Stem Cell Signaling RT 2 Profiler PCR Array analysis indicated that As 2 O 3 down-regulated the expression of GLI1 and PTCH1 in CD133 + Huh7-wt cells.

    Journal: Journal of Hematology & Oncology

    Article Title: Arsenic trioxide induces differentiation of CD133+ hepatocellular carcinoma cells and prolongs posthepatectomy survival by targeting GLI1 expression in a mouse model

    doi: 10.1186/1756-8722-7-28

    Figure Lengend Snippet: The results of Human Stem Cell Signaling RT 2 Profiler PCR Array analysis indicated that As 2 O 3 down-regulated the expression of GLI1 and PTCH1 in CD133 + Huh7-wt cells.

    Article Snippet: Huh7-LV-shGLI1 and Hep3B-LV-shGLI1 cells were obtained by infecting CD133+ Huh7-wt and CD133+ Hep3B-wt with a lentiviral vector encoding short hairpin RNA for GLI1 (sc-37911-V, Santa Cruz Biotechnology, Santa Cruz, CA, USA) to silence its expression.

    Techniques: Polymerase Chain Reaction, Expressing

    As 2 O 3 down-regulated GLI1 expression via the Hedgehog signaling pathway. (A) , As 2 O 3 decreased GLI1 protein expression in CD133 + Huh7-wt and CD133 + Hep3B-wt cells, as indicated by Western blotting analysis. (B) , Short hairpin RNA knockdown of GLI1 expression decreased CD133 expression in CD133 + Huh7-wt and CD133 + Hep3B-wt cells; similarly, up-regulation of GLI1 expression increased CD133 expression (wt = CD133 + Huh7-wt and CD133 + Hep3B-wt; control = Huh7-LV-shNon, Hep3B-LV-shNon, and Huh7-LV-oeNon, Hep3B-LV-oeNon; sh = Huh7-LV-shGLI1 and Hep3B-LV-shGLI1; oe = Huh7-LV-oeGLI1 and Hep3B-LV-oeGLI1). (C) , Huh7-LV-shGLI1 and Hep3B-LV-shGLI1 cells were treated with 4 μM As 2 O 3 for 48 hours, the expression of CD133 did not decrease significantly.

    Journal: Journal of Hematology & Oncology

    Article Title: Arsenic trioxide induces differentiation of CD133+ hepatocellular carcinoma cells and prolongs posthepatectomy survival by targeting GLI1 expression in a mouse model

    doi: 10.1186/1756-8722-7-28

    Figure Lengend Snippet: As 2 O 3 down-regulated GLI1 expression via the Hedgehog signaling pathway. (A) , As 2 O 3 decreased GLI1 protein expression in CD133 + Huh7-wt and CD133 + Hep3B-wt cells, as indicated by Western blotting analysis. (B) , Short hairpin RNA knockdown of GLI1 expression decreased CD133 expression in CD133 + Huh7-wt and CD133 + Hep3B-wt cells; similarly, up-regulation of GLI1 expression increased CD133 expression (wt = CD133 + Huh7-wt and CD133 + Hep3B-wt; control = Huh7-LV-shNon, Hep3B-LV-shNon, and Huh7-LV-oeNon, Hep3B-LV-oeNon; sh = Huh7-LV-shGLI1 and Hep3B-LV-shGLI1; oe = Huh7-LV-oeGLI1 and Hep3B-LV-oeGLI1). (C) , Huh7-LV-shGLI1 and Hep3B-LV-shGLI1 cells were treated with 4 μM As 2 O 3 for 48 hours, the expression of CD133 did not decrease significantly.

    Article Snippet: Huh7-LV-shGLI1 and Hep3B-LV-shGLI1 cells were obtained by infecting CD133+ Huh7-wt and CD133+ Hep3B-wt with a lentiviral vector encoding short hairpin RNA for GLI1 (sc-37911-V, Santa Cruz Biotechnology, Santa Cruz, CA, USA) to silence its expression.

    Techniques: Expressing, Western Blot, shRNA

    Trek-1 deficiency increases MLE-12 cell proliferation. A : increase in absolute cell numbers in Trek-1 shRNA-transfected cells after 48 h of culture at room air ( P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Regulation and function of the two-pore-domain (K2P) potassium channel Trek-1 in alveolar epithelial cells

    doi: 10.1152/ajplung.00078.2011

    Figure Lengend Snippet: Trek-1 deficiency increases MLE-12 cell proliferation. A : increase in absolute cell numbers in Trek-1 shRNA-transfected cells after 48 h of culture at room air ( P

    Article Snippet: A stable Trek-1 knockdown cell line was developed in our laboratory using commercially available Trek-1 shRNA Lentiviral particles (cat. no. SC-37181-V; control scrambled shRNA cat. no. 108080; Santa Cruz Biotechnology, Santa Cruz, CA) following the manufacturer's instructions.

    Techniques: shRNA, Transfection

    Targeted knockdown of Trek-1 expression in shRNA-transfected MLE-12 cells. A : decreased Trek-1 expression in shRNA-treated cells in a representative Western blot and a 50% decrease in Trek-1 expression using densitometry analysis ( n = 10, * P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Regulation and function of the two-pore-domain (K2P) potassium channel Trek-1 in alveolar epithelial cells

    doi: 10.1152/ajplung.00078.2011

    Figure Lengend Snippet: Targeted knockdown of Trek-1 expression in shRNA-transfected MLE-12 cells. A : decreased Trek-1 expression in shRNA-treated cells in a representative Western blot and a 50% decrease in Trek-1 expression using densitometry analysis ( n = 10, * P

    Article Snippet: A stable Trek-1 knockdown cell line was developed in our laboratory using commercially available Trek-1 shRNA Lentiviral particles (cat. no. SC-37181-V; control scrambled shRNA cat. no. 108080; Santa Cruz Biotechnology, Santa Cruz, CA) following the manufacturer's instructions.

    Techniques: Expressing, shRNA, Transfection, Western Blot

    Ebola virus (EBOV)–induced extracellular expression of phosphatidylserine (PtdSer) is dependent on transmembrane protein 16F (TMEM16F) function. Huh7 cells were transduced with lentiviral vectors encoding scrambled short hairpin RNA (shRNA) (Scr) or shRNAs specific for TMEM16F and infected with EBOV at a multiplicity of infection of 1 plaque-forming unit/cell. A, B, Translocation of PtdSer to the outer leaflet of the plasma membrane in EBOV-infected Huh7/shScramble ( A ) or Huh7/shTMEM16F ( B ) cells. Targeted knockdown of TMEM16F results in decreased levels of plasma membrane-associated PtdSer (scale bar represents 30 μm). C–E, Imaging flow cytometry analysis of mock-infected and EBOV-infected shRNA cell lines. C, Representative images of mock-infected and EBOV-infected Huh7/shScramble and Huh7/shTMEM16F cell lines. The merged image is based on glycoprotein (GP), annexin V, and 4′,6-diamidino-2-phenylindole (DAPI). D, Relative levels of EBOV GP ( left ) and annexin V staining ( right for gating strategy. Panels A and B are representative of 1 of 7 and panels C and D are representative of 1 of 3 independent experiments. Abbreviations: BF, bright field; SSC, side scatter.

    Journal: The Journal of Infectious Diseases

    Article Title: Role of Transmembrane Protein 16F in the Incorporation of Phosphatidylserine Into Budding Ebola Virus Virions

    doi: 10.1093/infdis/jiy485

    Figure Lengend Snippet: Ebola virus (EBOV)–induced extracellular expression of phosphatidylserine (PtdSer) is dependent on transmembrane protein 16F (TMEM16F) function. Huh7 cells were transduced with lentiviral vectors encoding scrambled short hairpin RNA (shRNA) (Scr) or shRNAs specific for TMEM16F and infected with EBOV at a multiplicity of infection of 1 plaque-forming unit/cell. A, B, Translocation of PtdSer to the outer leaflet of the plasma membrane in EBOV-infected Huh7/shScramble ( A ) or Huh7/shTMEM16F ( B ) cells. Targeted knockdown of TMEM16F results in decreased levels of plasma membrane-associated PtdSer (scale bar represents 30 μm). C–E, Imaging flow cytometry analysis of mock-infected and EBOV-infected shRNA cell lines. C, Representative images of mock-infected and EBOV-infected Huh7/shScramble and Huh7/shTMEM16F cell lines. The merged image is based on glycoprotein (GP), annexin V, and 4′,6-diamidino-2-phenylindole (DAPI). D, Relative levels of EBOV GP ( left ) and annexin V staining ( right for gating strategy. Panels A and B are representative of 1 of 7 and panels C and D are representative of 1 of 3 independent experiments. Abbreviations: BF, bright field; SSC, side scatter.

    Article Snippet: Lentiviral vectors targeting TMEM16F and XKr8 (both from Santa Cruz Biotechnology) and scrambled short hairpin RNAs (shRNAs) (Thermo Fisher Scientific) were used to transduce Huh7 cells in 6-well plates.

    Techniques: Expressing, Transduction, shRNA, Infection, Translocation Assay, Imaging, Flow Cytometry, Cytometry, Staining