Journal: Biomedicines

Article Title: Echinacoside Alleviates Cisplatin-Induced Acute Kidney Injury by Regulating Bap1 to Inhibit Ferroptosis

doi: 10.3390/biomedicines14092127

Figure Lengend Snippet: ECH restores Slc7a11/GPX4 axis to suppress ferroptosis in vitro. ( A ) Representative C11 BODIPY staining images of HK-2 cells and quantitative analysis of oxidized lipid fluorescence intensity. ( B ) SOD levels in HK-2 cells. ( C ) GSH levels in HK-2 cells. ( D ) MDA levels in HK-2 cells. ( E ) Iron content in HK-2 cells. ( F ) Western blot analysis of Slc7a11 and GPX4 proteins in HK-2 cells. ** p < 0.01 vs. control group; # p < 0.05 vs. cisplatin group.

Article Snippet: After membrane blocking, primary antibodies targeting KIM-1 (A24493, ABclonal, Wuhan, China), NGAL (A2092, ABclonal, Wuhan, China), GPX4 (A27995, ABclonal, Wuhan, China), Slc7a11 (A25302, ABclonal, Wuhan, China), Bap1 (A6533SP, ABclonal, Wuhan, China) and the internal reference GAPDH (A19056, ABclonal, Wuhan, China) were incubated overnight at 4 °C, followed by incubation with corresponding secondary antibodies.

Techniques: In Vitro, Staining, Analysis, Fluorescence, Western Blot, Control

Journal: Biomedicines

Article Title: Echinacoside Alleviates Cisplatin-Induced Acute Kidney Injury by Regulating Bap1 to Inhibit Ferroptosis

doi: 10.3390/biomedicines14092127

Figure Lengend Snippet: ECH attenuates mitochondrial ferroptotic damage in vivo. ( A ) Representative GPX4 immunofluorescence staining images of kidney tissues and quantitative analysis of fluorescence intensity. ( B ) Representative TEM images of renal tubular mitochondria and quantitative analysis of mitochondrial aspect ratio. ( C ) GSH levels in kidney tissues. ( D ) GSH/GSSG ratio in kidney tissues. ( E ) MDA levels in kidney tissues. ( F ) Ferrous iron levels in kidney tissues. ( G ) Western blot analysis of Slc7a11 and GPX4 proteins in kidney tissues. ** p < 0.01 vs. control group; # p < 0.05 vs. cisplatin group. n = 3 mice per group.

Article Snippet: After membrane blocking, primary antibodies targeting KIM-1 (A24493, ABclonal, Wuhan, China), NGAL (A2092, ABclonal, Wuhan, China), GPX4 (A27995, ABclonal, Wuhan, China), Slc7a11 (A25302, ABclonal, Wuhan, China), Bap1 (A6533SP, ABclonal, Wuhan, China) and the internal reference GAPDH (A19056, ABclonal, Wuhan, China) were incubated overnight at 4 °C, followed by incubation with corresponding secondary antibodies.

Techniques: In Vivo, Immunofluorescence, Staining, Analysis, Fluorescence, Western Blot, Control

Journal: Biomedicines

Article Title: Echinacoside Alleviates Cisplatin-Induced Acute Kidney Injury by Regulating Bap1 to Inhibit Ferroptosis

doi: 10.3390/biomedicines14092127

Figure Lengend Snippet: Overexpression of Bap1 largely abrogates renoprotective effects of ECH. ( A ) Relative Bap1 mRNA levels in HK-2 cells. ( B ) Western blot and quantitative analysis of Bap1 protein in HK-2 cells. ( C ) Representative PI staining images of HK-2 cells and quantitative analysis of the PI-positive area. ( D ) Western blot analysis of Bap1, Slc7a11 and GPX4 proteins in HK-2 cells. ** p < 0.01 vs. OE-Ctrl group ( A , B ) or cisplatin group ( C , D ); # p < 0.05 vs. Cisplatin + ECH group.

Article Snippet: After membrane blocking, primary antibodies targeting KIM-1 (A24493, ABclonal, Wuhan, China), NGAL (A2092, ABclonal, Wuhan, China), GPX4 (A27995, ABclonal, Wuhan, China), Slc7a11 (A25302, ABclonal, Wuhan, China), Bap1 (A6533SP, ABclonal, Wuhan, China) and the internal reference GAPDH (A19056, ABclonal, Wuhan, China) were incubated overnight at 4 °C, followed by incubation with corresponding secondary antibodies.

Techniques: Over Expression, Western Blot, Analysis, Staining