skf96365  (Alomone Labs)


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    Structured Review

    Alomone Labs skf96365
    Cholinergic-associated shift in the timing of firing on ramp is mediated by TRP channels, but notably not TRPC channels. A. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (A1), CCh (2 μM, A2), and CCh + FFA (100 μM, A3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (A4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). B. Same as in A, but for dendritic injection and recordings. Summary data (B4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). Open circles connected by dashed lines represent indices for individual cells, over all three treatments. Black squares with error bars represent group averages ± SEM. C. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (C1), CCh (2 μM, C2), and CCh + <t>SKF96365</t> (50 μM, C3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (C4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). D. Same as in C, but for dendritic injection and recordings. Summary data (D4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). *** p < 0.0005, ** p = 0.001, * p = 0.02, n.s. not significant.
    Skf96365, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Cholinergic Modulation Shifts the Response of CA1 Pyramidal Cells to Depolarizing Ramps via TRPM4 Channels with Implications for Place Field Firing"

    Article Title: Cholinergic Modulation Shifts the Response of CA1 Pyramidal Cells to Depolarizing Ramps via TRPM4 Channels with Implications for Place Field Firing

    Journal: bioRxiv

    doi: 10.1101/2022.10.24.513511

    Cholinergic-associated shift in the timing of firing on ramp is mediated by TRP channels, but notably not TRPC channels. A. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (A1), CCh (2 μM, A2), and CCh + FFA (100 μM, A3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (A4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). B. Same as in A, but for dendritic injection and recordings. Summary data (B4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). Open circles connected by dashed lines represent indices for individual cells, over all three treatments. Black squares with error bars represent group averages ± SEM. C. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (C1), CCh (2 μM, C2), and CCh + SKF96365 (50 μM, C3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (C4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). D. Same as in C, but for dendritic injection and recordings. Summary data (D4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). *** p < 0.0005, ** p = 0.001, * p = 0.02, n.s. not significant.
    Figure Legend Snippet: Cholinergic-associated shift in the timing of firing on ramp is mediated by TRP channels, but notably not TRPC channels. A. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (A1), CCh (2 μM, A2), and CCh + FFA (100 μM, A3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (A4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). B. Same as in A, but for dendritic injection and recordings. Summary data (B4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). Open circles connected by dashed lines represent indices for individual cells, over all three treatments. Black squares with error bars represent group averages ± SEM. C. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (C1), CCh (2 μM, C2), and CCh + SKF96365 (50 μM, C3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (C4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). D. Same as in C, but for dendritic injection and recordings. Summary data (D4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). *** p < 0.0005, ** p = 0.001, * p = 0.02, n.s. not significant.

    Techniques Used: Injection

    skf96365  (Alomone Labs)


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    Structured Review

    Alomone Labs skf96365
    Cholinergic-associated shift in the timing of firing on ramp is mediated by TRP channels, but notably not TRPC channels. A. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (A1), CCh (2 μM, A2), and CCh + FFA (100 μM, A3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (A4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). B. Same as in A, but for dendritic injection and recordings. Summary data (B4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). Open circles connected by dashed lines represent indices for individual cells, over all three treatments. Black squares with error bars represent group averages ± SEM. C. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (C1), CCh (2 μM, C2), and CCh + <t>SKF96365</t> (50 μM, C3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (C4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). D. Same as in C, but for dendritic injection and recordings. Summary data (D4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). *** p < 0.0005, ** p = 0.001, * p = 0.02, n.s. not significant.
    Skf96365, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    Images

    1) Product Images from "Cholinergic Modulation Shifts the Response of CA1 Pyramidal Cells to Depolarizing Ramps via TRPM4 Channels with Implications for Place Field Firing"

    Article Title: Cholinergic Modulation Shifts the Response of CA1 Pyramidal Cells to Depolarizing Ramps via TRPM4 Channels with Implications for Place Field Firing

    Journal: bioRxiv

    doi: 10.1101/2022.10.24.513511

    Cholinergic-associated shift in the timing of firing on ramp is mediated by TRP channels, but notably not TRPC channels. A. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (A1), CCh (2 μM, A2), and CCh + FFA (100 μM, A3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (A4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). B. Same as in A, but for dendritic injection and recordings. Summary data (B4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). Open circles connected by dashed lines represent indices for individual cells, over all three treatments. Black squares with error bars represent group averages ± SEM. C. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (C1), CCh (2 μM, C2), and CCh + SKF96365 (50 μM, C3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (C4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). D. Same as in C, but for dendritic injection and recordings. Summary data (D4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). *** p < 0.0005, ** p = 0.001, * p = 0.02, n.s. not significant.
    Figure Legend Snippet: Cholinergic-associated shift in the timing of firing on ramp is mediated by TRP channels, but notably not TRPC channels. A. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (A1), CCh (2 μM, A2), and CCh + FFA (100 μM, A3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (A4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). B. Same as in A, but for dendritic injection and recordings. Summary data (B4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). Open circles connected by dashed lines represent indices for individual cells, over all three treatments. Black squares with error bars represent group averages ± SEM. C. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (C1), CCh (2 μM, C2), and CCh + SKF96365 (50 μM, C3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (C4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). D. Same as in C, but for dendritic injection and recordings. Summary data (D4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). *** p < 0.0005, ** p = 0.001, * p = 0.02, n.s. not significant.

    Techniques Used: Injection

    skf96365  (Alomone Labs)


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    Alomone Labs skf96365
    NT-3 generated long-range Ca 2+ signaling from the axon to the cell body. a Long-range Ca 2+ wave. The relative change in the Cal-520 emission ratio (defined as R) was used as a measure of changes in Ca 2+ concentration. The pseudocolored images represent R after local application of PBS ( top ) or NT-3 ( bottom ) to the axon ( arrow ). Scale bars, 50 μm. b The mean amplitude of R treat / R 0 for 90 s during local application of NT-3 in the presence of the indicated inhibitors (PBS = 10, NT-3 = 16, xestospongin C = 9, ryanodine = 15, dantrolene = 18, <t>SKF96365</t> = 12 neurons from three independent experiments). c , d The axon was exposed to NT-3 in the presence of the indicated inhibitors, and then minor neurite outgrowth (PBS = 21, NT-3 = 21, xestospongin C = 26, ryanodine = 25, dantrolene = 25, SKF96365 = 24 neurites from three independent experiments) c and axonal outgrowth (PBS = 7, NT-3 = 9, xestospongin C = 9, ryanodine = 9, dantrolene = 8, SKF96365 = 8 neurons from three independent experiments) d were measured. e Local application of NT-3 increased the quantity of phospho-CaMKI in the cell body. After local application of PBS ( top ) or NT-3 ( bottom ), hippocampal neurons were immunostained with antibodies against CaMKI ( green ) and phospho-Thr177 of CaMKI ( magenta ). The merged images ( right panels ) are shown. The graph plots the fluorescence intensities of total CaMKI ( green ) and CaMKI phosphorylated at Thr177 ( magenta ) and in the line. Scale bars, 20 μm. f NT-3-induced minor neurite retraction was abolished by Ca 2+ signaling inhibitors. The axon was exposed to NT-3 in the presence of the indicated inhibitors, and minor neurite outgrowth was measured (PBS = 27, NT-3 = 31, BAPTA = 47, STO-609 = 45, KN-93 = 40 neurites from three independent experiments). g , h Local application of indicated inhibitors to the axon. Minor neurite outgrowth (DMSO = 42, xestospongin C = 32, ryanodine = 37, dantrolene = 35, SKF96365 = 32 neurons from three independent experiments) g and axonal outgrowth (DMSO = 14, xestospongin C = 11, ryanodine = 13, dantrolene = 13, SKF96365 = 12 neurons from three independent experiments) h were measured. Error bars represent SEM. * P < 0.05 and ** P < 0.01
    Skf96365, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    skf96365 - by Bioz Stars, 2023-02
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    Images

    1) Product Images from "Discovery of long-range inhibitory signaling to ensure single axon formation"

    Article Title: Discovery of long-range inhibitory signaling to ensure single axon formation

    Journal: Nature Communications

    doi: 10.1038/s41467-017-00044-2

    NT-3 generated long-range Ca 2+ signaling from the axon to the cell body. a Long-range Ca 2+ wave. The relative change in the Cal-520 emission ratio (defined as R) was used as a measure of changes in Ca 2+ concentration. The pseudocolored images represent R after local application of PBS ( top ) or NT-3 ( bottom ) to the axon ( arrow ). Scale bars, 50 μm. b The mean amplitude of R treat / R 0 for 90 s during local application of NT-3 in the presence of the indicated inhibitors (PBS = 10, NT-3 = 16, xestospongin C = 9, ryanodine = 15, dantrolene = 18, SKF96365 = 12 neurons from three independent experiments). c , d The axon was exposed to NT-3 in the presence of the indicated inhibitors, and then minor neurite outgrowth (PBS = 21, NT-3 = 21, xestospongin C = 26, ryanodine = 25, dantrolene = 25, SKF96365 = 24 neurites from three independent experiments) c and axonal outgrowth (PBS = 7, NT-3 = 9, xestospongin C = 9, ryanodine = 9, dantrolene = 8, SKF96365 = 8 neurons from three independent experiments) d were measured. e Local application of NT-3 increased the quantity of phospho-CaMKI in the cell body. After local application of PBS ( top ) or NT-3 ( bottom ), hippocampal neurons were immunostained with antibodies against CaMKI ( green ) and phospho-Thr177 of CaMKI ( magenta ). The merged images ( right panels ) are shown. The graph plots the fluorescence intensities of total CaMKI ( green ) and CaMKI phosphorylated at Thr177 ( magenta ) and in the line. Scale bars, 20 μm. f NT-3-induced minor neurite retraction was abolished by Ca 2+ signaling inhibitors. The axon was exposed to NT-3 in the presence of the indicated inhibitors, and minor neurite outgrowth was measured (PBS = 27, NT-3 = 31, BAPTA = 47, STO-609 = 45, KN-93 = 40 neurites from three independent experiments). g , h Local application of indicated inhibitors to the axon. Minor neurite outgrowth (DMSO = 42, xestospongin C = 32, ryanodine = 37, dantrolene = 35, SKF96365 = 32 neurons from three independent experiments) g and axonal outgrowth (DMSO = 14, xestospongin C = 11, ryanodine = 13, dantrolene = 13, SKF96365 = 12 neurons from three independent experiments) h were measured. Error bars represent SEM. * P < 0.05 and ** P < 0.01
    Figure Legend Snippet: NT-3 generated long-range Ca 2+ signaling from the axon to the cell body. a Long-range Ca 2+ wave. The relative change in the Cal-520 emission ratio (defined as R) was used as a measure of changes in Ca 2+ concentration. The pseudocolored images represent R after local application of PBS ( top ) or NT-3 ( bottom ) to the axon ( arrow ). Scale bars, 50 μm. b The mean amplitude of R treat / R 0 for 90 s during local application of NT-3 in the presence of the indicated inhibitors (PBS = 10, NT-3 = 16, xestospongin C = 9, ryanodine = 15, dantrolene = 18, SKF96365 = 12 neurons from three independent experiments). c , d The axon was exposed to NT-3 in the presence of the indicated inhibitors, and then minor neurite outgrowth (PBS = 21, NT-3 = 21, xestospongin C = 26, ryanodine = 25, dantrolene = 25, SKF96365 = 24 neurites from three independent experiments) c and axonal outgrowth (PBS = 7, NT-3 = 9, xestospongin C = 9, ryanodine = 9, dantrolene = 8, SKF96365 = 8 neurons from three independent experiments) d were measured. e Local application of NT-3 increased the quantity of phospho-CaMKI in the cell body. After local application of PBS ( top ) or NT-3 ( bottom ), hippocampal neurons were immunostained with antibodies against CaMKI ( green ) and phospho-Thr177 of CaMKI ( magenta ). The merged images ( right panels ) are shown. The graph plots the fluorescence intensities of total CaMKI ( green ) and CaMKI phosphorylated at Thr177 ( magenta ) and in the line. Scale bars, 20 μm. f NT-3-induced minor neurite retraction was abolished by Ca 2+ signaling inhibitors. The axon was exposed to NT-3 in the presence of the indicated inhibitors, and minor neurite outgrowth was measured (PBS = 27, NT-3 = 31, BAPTA = 47, STO-609 = 45, KN-93 = 40 neurites from three independent experiments). g , h Local application of indicated inhibitors to the axon. Minor neurite outgrowth (DMSO = 42, xestospongin C = 32, ryanodine = 37, dantrolene = 35, SKF96365 = 32 neurons from three independent experiments) g and axonal outgrowth (DMSO = 14, xestospongin C = 11, ryanodine = 13, dantrolene = 13, SKF96365 = 12 neurons from three independent experiments) h were measured. Error bars represent SEM. * P < 0.05 and ** P < 0.01

    Techniques Used: Generated, Concentration Assay, Fluorescence

    skf96365 1 2 4 methoxyphenyl  (Alomone Labs)


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    Alomone Labs skf96365 1 2 4 methoxyphenyl
    Skf96365 1 2 4 Methoxyphenyl, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    skf96365 1 2 4 methoxyphenyl  (Alomone Labs)


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    Alomone Labs skf96365 1 2 4 methoxyphenyl
    Skf96365 1 2 4 Methoxyphenyl, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    skf96365 1 2 4 methoxyphenyl  (Alomone Labs)


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    Alomone Labs skf96365 1 2 4 methoxyphenyl
    Skf96365 1 2 4 Methoxyphenyl, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    skf96365 1 2 4 methoxyphenyl  (Alomone Labs)


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    Alomone Labs skf96365 1 2 4 methoxyphenyl
    Skf96365 1 2 4 Methoxyphenyl, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    skf96365 1 2 4 methoxyphenyl  (Alomone Labs)


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    Alomone Labs skf96365 1 2 4 methoxyphenyl
    Skf96365 1 2 4 Methoxyphenyl, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    skf96365  (Alomone Labs)


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    Alomone Labs skf96365
    Effects of TRPC channel blockers on the kisspeptin-induced inward currents at −60 mV. A, A representative recording showing that 2-APB (100 μm), which had very little effect on basal holding current, potently blocked the kisspeptin (100 nm)-evoked inward current. B, Mean I–V relationship of the kisspeptin-sensitive current in the presence of 2-APB reversed at −90 mV (n = 4), clearly indicating that a Kir channel was inhibited by kisspeptin. C, A representative recording showing that 2-APB (100 μm) applied after kisspeptin also strongly blocked the kisspeptin-evoked inward current. D, Summary of the effect of different TRPC channel blockers (100 μm La3+, 100 μm 2-APB, 30 μm <t>SKF96365,</t> 250 μm Cd2+, 100 μm flufenamic acid) on the kisspeptin-induced inward currents at −60 mV. Blockers were applied 5–7 min before or after the application of kisspeptin (100 nm). The percentage inhibition for the different blockers was as follows: 17.4% for 100 μm La3+, 83.6% for 100 μm 2-APB, 50% for 30 μm SKF, 68.7% for 250 μm Cd2+, and 89.6% for 100 μm FFA. **p < 0.01 and ***p < 0.001, significantly different from the control group. Cell numbers tested are indicated. Error bars indicate SEM.
    Skf96365, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Kisspeptin Depolarizes Gonadotropin-Releasing Hormone Neurons through Activation of TRPC-Like Cationic Channels"

    Article Title: Kisspeptin Depolarizes Gonadotropin-Releasing Hormone Neurons through Activation of TRPC-Like Cationic Channels

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.5352-07.2008

    Effects of TRPC channel blockers on the kisspeptin-induced inward currents at −60 mV. A, A representative recording showing that 2-APB (100 μm), which had very little effect on basal holding current, potently blocked the kisspeptin (100 nm)-evoked inward current. B, Mean I–V relationship of the kisspeptin-sensitive current in the presence of 2-APB reversed at −90 mV (n = 4), clearly indicating that a Kir channel was inhibited by kisspeptin. C, A representative recording showing that 2-APB (100 μm) applied after kisspeptin also strongly blocked the kisspeptin-evoked inward current. D, Summary of the effect of different TRPC channel blockers (100 μm La3+, 100 μm 2-APB, 30 μm SKF96365, 250 μm Cd2+, 100 μm flufenamic acid) on the kisspeptin-induced inward currents at −60 mV. Blockers were applied 5–7 min before or after the application of kisspeptin (100 nm). The percentage inhibition for the different blockers was as follows: 17.4% for 100 μm La3+, 83.6% for 100 μm 2-APB, 50% for 30 μm SKF, 68.7% for 250 μm Cd2+, and 89.6% for 100 μm FFA. **p < 0.01 and ***p < 0.001, significantly different from the control group. Cell numbers tested are indicated. Error bars indicate SEM.
    Figure Legend Snippet: Effects of TRPC channel blockers on the kisspeptin-induced inward currents at −60 mV. A, A representative recording showing that 2-APB (100 μm), which had very little effect on basal holding current, potently blocked the kisspeptin (100 nm)-evoked inward current. B, Mean I–V relationship of the kisspeptin-sensitive current in the presence of 2-APB reversed at −90 mV (n = 4), clearly indicating that a Kir channel was inhibited by kisspeptin. C, A representative recording showing that 2-APB (100 μm) applied after kisspeptin also strongly blocked the kisspeptin-evoked inward current. D, Summary of the effect of different TRPC channel blockers (100 μm La3+, 100 μm 2-APB, 30 μm SKF96365, 250 μm Cd2+, 100 μm flufenamic acid) on the kisspeptin-induced inward currents at −60 mV. Blockers were applied 5–7 min before or after the application of kisspeptin (100 nm). The percentage inhibition for the different blockers was as follows: 17.4% for 100 μm La3+, 83.6% for 100 μm 2-APB, 50% for 30 μm SKF, 68.7% for 250 μm Cd2+, and 89.6% for 100 μm FFA. **p < 0.01 and ***p < 0.001, significantly different from the control group. Cell numbers tested are indicated. Error bars indicate SEM.

    Techniques Used: Inhibition

    skf96365  (Alomone Labs)


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    Structured Review

    Alomone Labs skf96365
    Effects of TRPC channel blockers on the kisspeptin-induced inward currents at −60 mV. A, A representative recording showing that 2-APB (100 μm), which had very little effect on basal holding current, potently blocked the kisspeptin (100 nm)-evoked inward current. B, Mean I–V relationship of the kisspeptin-sensitive current in the presence of 2-APB reversed at −90 mV (n = 4), clearly indicating that a Kir channel was inhibited by kisspeptin. C, A representative recording showing that 2-APB (100 μm) applied after kisspeptin also strongly blocked the kisspeptin-evoked inward current. D, Summary of the effect of different TRPC channel blockers (100 μm La3+, 100 μm 2-APB, 30 μm <t>SKF96365,</t> 250 μm Cd2+, 100 μm flufenamic acid) on the kisspeptin-induced inward currents at −60 mV. Blockers were applied 5–7 min before or after the application of kisspeptin (100 nm). The percentage inhibition for the different blockers was as follows: 17.4% for 100 μm La3+, 83.6% for 100 μm 2-APB, 50% for 30 μm SKF, 68.7% for 250 μm Cd2+, and 89.6% for 100 μm FFA. **p < 0.01 and ***p < 0.001, significantly different from the control group. Cell numbers tested are indicated. Error bars indicate SEM.
    Skf96365, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Kisspeptin Depolarizes Gonadotropin-Releasing Hormone Neurons through Activation of TRPC-Like Cationic Channels"

    Article Title: Kisspeptin Depolarizes Gonadotropin-Releasing Hormone Neurons through Activation of TRPC-Like Cationic Channels

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.5352-07.2008

    Effects of TRPC channel blockers on the kisspeptin-induced inward currents at −60 mV. A, A representative recording showing that 2-APB (100 μm), which had very little effect on basal holding current, potently blocked the kisspeptin (100 nm)-evoked inward current. B, Mean I–V relationship of the kisspeptin-sensitive current in the presence of 2-APB reversed at −90 mV (n = 4), clearly indicating that a Kir channel was inhibited by kisspeptin. C, A representative recording showing that 2-APB (100 μm) applied after kisspeptin also strongly blocked the kisspeptin-evoked inward current. D, Summary of the effect of different TRPC channel blockers (100 μm La3+, 100 μm 2-APB, 30 μm SKF96365, 250 μm Cd2+, 100 μm flufenamic acid) on the kisspeptin-induced inward currents at −60 mV. Blockers were applied 5–7 min before or after the application of kisspeptin (100 nm). The percentage inhibition for the different blockers was as follows: 17.4% for 100 μm La3+, 83.6% for 100 μm 2-APB, 50% for 30 μm SKF, 68.7% for 250 μm Cd2+, and 89.6% for 100 μm FFA. **p < 0.01 and ***p < 0.001, significantly different from the control group. Cell numbers tested are indicated. Error bars indicate SEM.
    Figure Legend Snippet: Effects of TRPC channel blockers on the kisspeptin-induced inward currents at −60 mV. A, A representative recording showing that 2-APB (100 μm), which had very little effect on basal holding current, potently blocked the kisspeptin (100 nm)-evoked inward current. B, Mean I–V relationship of the kisspeptin-sensitive current in the presence of 2-APB reversed at −90 mV (n = 4), clearly indicating that a Kir channel was inhibited by kisspeptin. C, A representative recording showing that 2-APB (100 μm) applied after kisspeptin also strongly blocked the kisspeptin-evoked inward current. D, Summary of the effect of different TRPC channel blockers (100 μm La3+, 100 μm 2-APB, 30 μm SKF96365, 250 μm Cd2+, 100 μm flufenamic acid) on the kisspeptin-induced inward currents at −60 mV. Blockers were applied 5–7 min before or after the application of kisspeptin (100 nm). The percentage inhibition for the different blockers was as follows: 17.4% for 100 μm La3+, 83.6% for 100 μm 2-APB, 50% for 30 μm SKF, 68.7% for 250 μm Cd2+, and 89.6% for 100 μm FFA. **p < 0.01 and ***p < 0.001, significantly different from the control group. Cell numbers tested are indicated. Error bars indicate SEM.

    Techniques Used: Inhibition

    skf96365  (Alomone Labs)


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    Alomone Labs skf96365
    Effects of TRPC channel blockers on the kisspeptin-induced inward currents at −60 mV. A, A representative recording showing that 2-APB (100 μm), which had very little effect on basal holding current, potently blocked the kisspeptin (100 nm)-evoked inward current. B, Mean I–V relationship of the kisspeptin-sensitive current in the presence of 2-APB reversed at −90 mV (n = 4), clearly indicating that a Kir channel was inhibited by kisspeptin. C, A representative recording showing that 2-APB (100 μm) applied after kisspeptin also strongly blocked the kisspeptin-evoked inward current. D, Summary of the effect of different TRPC channel blockers (100 μm La3+, 100 μm 2-APB, 30 μm <t>SKF96365,</t> 250 μm Cd2+, 100 μm flufenamic acid) on the kisspeptin-induced inward currents at −60 mV. Blockers were applied 5–7 min before or after the application of kisspeptin (100 nm). The percentage inhibition for the different blockers was as follows: 17.4% for 100 μm La3+, 83.6% for 100 μm 2-APB, 50% for 30 μm SKF, 68.7% for 250 μm Cd2+, and 89.6% for 100 μm FFA. **p < 0.01 and ***p < 0.001, significantly different from the control group. Cell numbers tested are indicated. Error bars indicate SEM.
    Skf96365, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/skf96365/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    skf96365 - by Bioz Stars, 2023-02
    86/100 stars

    Images

    1) Product Images from "Kisspeptin Depolarizes Gonadotropin-Releasing Hormone Neurons through Activation of TRPC-Like Cationic Channels"

    Article Title: Kisspeptin Depolarizes Gonadotropin-Releasing Hormone Neurons through Activation of TRPC-Like Cationic Channels

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.5352-07.2008

    Effects of TRPC channel blockers on the kisspeptin-induced inward currents at −60 mV. A, A representative recording showing that 2-APB (100 μm), which had very little effect on basal holding current, potently blocked the kisspeptin (100 nm)-evoked inward current. B, Mean I–V relationship of the kisspeptin-sensitive current in the presence of 2-APB reversed at −90 mV (n = 4), clearly indicating that a Kir channel was inhibited by kisspeptin. C, A representative recording showing that 2-APB (100 μm) applied after kisspeptin also strongly blocked the kisspeptin-evoked inward current. D, Summary of the effect of different TRPC channel blockers (100 μm La3+, 100 μm 2-APB, 30 μm SKF96365, 250 μm Cd2+, 100 μm flufenamic acid) on the kisspeptin-induced inward currents at −60 mV. Blockers were applied 5–7 min before or after the application of kisspeptin (100 nm). The percentage inhibition for the different blockers was as follows: 17.4% for 100 μm La3+, 83.6% for 100 μm 2-APB, 50% for 30 μm SKF, 68.7% for 250 μm Cd2+, and 89.6% for 100 μm FFA. **p < 0.01 and ***p < 0.001, significantly different from the control group. Cell numbers tested are indicated. Error bars indicate SEM.
    Figure Legend Snippet: Effects of TRPC channel blockers on the kisspeptin-induced inward currents at −60 mV. A, A representative recording showing that 2-APB (100 μm), which had very little effect on basal holding current, potently blocked the kisspeptin (100 nm)-evoked inward current. B, Mean I–V relationship of the kisspeptin-sensitive current in the presence of 2-APB reversed at −90 mV (n = 4), clearly indicating that a Kir channel was inhibited by kisspeptin. C, A representative recording showing that 2-APB (100 μm) applied after kisspeptin also strongly blocked the kisspeptin-evoked inward current. D, Summary of the effect of different TRPC channel blockers (100 μm La3+, 100 μm 2-APB, 30 μm SKF96365, 250 μm Cd2+, 100 μm flufenamic acid) on the kisspeptin-induced inward currents at −60 mV. Blockers were applied 5–7 min before or after the application of kisspeptin (100 nm). The percentage inhibition for the different blockers was as follows: 17.4% for 100 μm La3+, 83.6% for 100 μm 2-APB, 50% for 30 μm SKF, 68.7% for 250 μm Cd2+, and 89.6% for 100 μm FFA. **p < 0.01 and ***p < 0.001, significantly different from the control group. Cell numbers tested are indicated. Error bars indicate SEM.

    Techniques Used: Inhibition

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    Alomone Labs skf96365
    Cholinergic-associated shift in the timing of firing on ramp is mediated by TRP channels, but notably not TRPC channels. A. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (A1), CCh (2 μM, A2), and CCh + FFA (100 μM, A3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (A4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). B. Same as in A, but for dendritic injection and recordings. Summary data (B4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). Open circles connected by dashed lines represent indices for individual cells, over all three treatments. Black squares with error bars represent group averages ± SEM. C. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (C1), CCh (2 μM, C2), and CCh + <t>SKF96365</t> (50 μM, C3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (C4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). D. Same as in C, but for dendritic injection and recordings. Summary data (D4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). *** p < 0.0005, ** p = 0.001, * p = 0.02, n.s. not significant.
    Skf96365, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs skf96365 1 2 4 methoxyphenyl
    Cholinergic-associated shift in the timing of firing on ramp is mediated by TRP channels, but notably not TRPC channels. A. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (A1), CCh (2 μM, A2), and CCh + FFA (100 μM, A3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (A4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). B. Same as in A, but for dendritic injection and recordings. Summary data (B4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). Open circles connected by dashed lines represent indices for individual cells, over all three treatments. Black squares with error bars represent group averages ± SEM. C. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (C1), CCh (2 μM, C2), and CCh + <t>SKF96365</t> (50 μM, C3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (C4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). D. Same as in C, but for dendritic injection and recordings. Summary data (D4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). *** p < 0.0005, ** p = 0.001, * p = 0.02, n.s. not significant.
    Skf96365 1 2 4 Methoxyphenyl, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cholinergic-associated shift in the timing of firing on ramp is mediated by TRP channels, but notably not TRPC channels. A. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (A1), CCh (2 μM, A2), and CCh + FFA (100 μM, A3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (A4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). B. Same as in A, but for dendritic injection and recordings. Summary data (B4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). Open circles connected by dashed lines represent indices for individual cells, over all three treatments. Black squares with error bars represent group averages ± SEM. C. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (C1), CCh (2 μM, C2), and CCh + SKF96365 (50 μM, C3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (C4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). D. Same as in C, but for dendritic injection and recordings. Summary data (D4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). *** p < 0.0005, ** p = 0.001, * p = 0.02, n.s. not significant.

    Journal: bioRxiv

    Article Title: Cholinergic Modulation Shifts the Response of CA1 Pyramidal Cells to Depolarizing Ramps via TRPM4 Channels with Implications for Place Field Firing

    doi: 10.1101/2022.10.24.513511

    Figure Lengend Snippet: Cholinergic-associated shift in the timing of firing on ramp is mediated by TRP channels, but notably not TRPC channels. A. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (A1), CCh (2 μM, A2), and CCh + FFA (100 μM, A3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (A4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). B. Same as in A, but for dendritic injection and recordings. Summary data (B4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). Open circles connected by dashed lines represent indices for individual cells, over all three treatments. Black squares with error bars represent group averages ± SEM. C. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (C1), CCh (2 μM, C2), and CCh + SKF96365 (50 μM, C3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (C4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). D. Same as in C, but for dendritic injection and recordings. Summary data (D4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). *** p < 0.0005, ** p = 0.001, * p = 0.02, n.s. not significant.

    Article Snippet: Carbachol, 9-phenanthrol, and CBA were purchased from Tocris, CGP55845 from Abcam (Cambridge, MA), DL-APV from HelloBio (Princeton, NJ), NBQX, Gabazine, and SKF96365 from Alomone Labs (Jerusalem, Israel), and acetylcholine, flufenamic acid, and K4-BAPTA from Sigma (St. Louis, MO).

    Techniques: Injection